scholarly journals Antimicrobial Activity Exerted by Total Extracts of Germander

2019 ◽  
Vol 70 (9) ◽  
pp. 3242-3244
Author(s):  
Raul Chioibas ◽  
Razvan Susan ◽  
Monica Susan ◽  
Ovidiu Mederle ◽  
Delia Berceanu Vaduva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Fungal Species ◽  
Polyphenolic Compounds ◽  
Biologically Active ◽  
E Coli ◽  
Methanolic Extracts ◽  
Teucrium Polium ◽  
Active Substances

In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.

scholarly journals Optimization of Extraction of Polyphenolic Compounds from Medicinal Lungwort (Pulmonaria officinalis L.)

2020 ◽  
pp. 36-45
Author(s):  
Lyubov S. Dyshlyuk ◽  
Anastasia M. Fedorova ◽  
Vyacheslav F. Dolganyuk ◽  
Alexander Yu. Prosekov
Keyword(s):  
Antioxidant Activity ◽  
Alcohol Concentration ◽  
Polyphenolic Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
High Yield ◽  
Operating Parameters ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Active Substances

Medicinal plants are potential natural sources of biologically active substances that have geroprotective properties, slowing down the aging process. Vegetable raw materials are used as antioxidants in the food industry. Pulmonaria officinalis L. has a high content of biologically active substances. The purpose of this work is to determine the operating parameters for extracting the maximum amount of biologically active substances from Pulmonaria officinalis L. by extracting ethanol and finding sources of natural antioxidants.The antioxidant activity of several ethanol extracts of Pulmonaria officinalis L. – 30-70% with a step of 10 ethanol obtained from the dried root culture of the medicinal plant of biologically active substances was determined. The total content of polyphenols, flavonoids and proanthocyanidins was estimated. According to the data obtained, the relationship between the values determined for the biologically active substances presented and the values of antioxidant activity was revealed. Extracts of Pulmonaria officinalis L. showed a high yield of polyphenolic compounds-889.39±4.29 mcg of Gallic acid/ml, flavonoids-728.90±6.98 mcg of rutin/ml, proanthocyanidins-211.65±5.31 mcg of catechin/ml at an ethyl alcohol concentration of 60% and an extraction temperature of 50°C in 4 hours. The antioxidant activity using 2 methods: A spectrophotometric method DPPH (2,2-diphenyl-1-picrylhydrazyl) and the FRAP method (iron reducing/antioxidant power) was investigated. During DPPH spectrophotometric analysis, the antioxidant activity is equal to 86.96% for polyphenols, 75.47% for flavonoids, and 51.25% for proanthocyanidins. FRAP analysis showed that extracts with operating parameters had a pronounced antioxidant activity (tэ= 4 ч; Tэ= 50°C; Сэ= 60%)и (tэ= 5 ч; Tэ= 60°C; Сэ, = 70%).

scholarly journals ANTIMICROBIAL ACTIVITY OF OIL-BEARING PLANTS LAMIACEAE LINDL. TOWARDS ESCHERICHIA COLI

2016 ◽  
Vol 6 (01) ◽  
pp. 216-236
Author(s):  
L.A. Kotyuk
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Biologically Active ◽  
E Coli ◽  
Twofold Increase ◽  
Ethanol Extracts ◽  
Main Components ◽  
Active Substances

<p> </p><p>The paper relates to study of biological activity of 40% ethanol extracts of <em>Dracocephalum moldavica,</em> <em>Hyssopus officinalis</em>, <em>Satureja hortensis</em>, <em>Lophanthus anisatus</em> and <em>Monarda diduma</em>, grown in Ukrainian Polissya, against a pathogenic agent <em>Escherichia coli</em> UCM – B (ATCC 25922).</p><p>The research proves that ethanol extracts of <em>H. officinalis</em>, <em>D. moldavica, S. hortensis, L. anisatus</em> exert antimicrobial activity as the extracted substances provided a twofold increase in minimum bactericidal concentration (MBC) values against <em>E. coli. </em>Likewise, a twofold increase was observed in minimum inhibitory concentration (MIC) of <em>L. anisatus</em> ethanol extracts. As to <em>M. diduma </em>ethanol extracts, their inhibitory and bactericidal influence on <em>E. coli</em> was not registered.</p><p>Oil-bearing plants (family <em>Lamiaceae), </em>grown in Zhytomyr Polissya, are characterized by antimicrobial properties, attributed to biologically active substances that are formed and accumulated in the plant material. The main components of hyssop essential oil are isopinocamphone (44.43%), pinocamphone (35.49%), myrtenol (5.26 %), germacrene D (3.15 %), pulegone (2.93 %), bicyclogermacrene (1.35 %). In mint anise essential oil prevailed pulegone (59.19%), izomenton (14.34%), bicyclogermacrene (3,21 %), <em>β</em>-kariofilen (2,99 %), menton (2.21 %), 1,6-germacradien-5-ol (1.5 %), isopulegone (1.4 %), in summer savory – carvacrol (89.07%), g-terpinene (3.53%), <em>α</em>-thujone (1.7 %), camphor (1.48 %). The dominant components of moldavian dragonhead essential oil were geranial (26.19%) and neral (22.36%), 2-(1-hydroxy-1-isopropyl)-cyklopentanon (8.29 % ), 2,3-dehydro-1,8-cineole (6.87 %), 3-(1-hydroxy-1-isopropyl) cyklopentanon (6,51 %), nerol (4.74 %), 3-methyl-2-cyclohexane 1-on (2.13 %).<em></em></p><p>The paper draws attention to further more detailed study of ethanol extracts of hyssop, moldavian dragonhead, summer savory, mint anise with the aim of producing antibacterial herbal preparations.</p>

Substituted 6,7-dimethoxy-5-oxo-2,3,5,9b-tetrahydrothiazolo[2,3-a]isoindole- 3-1,1-dioxide Derivatives with Antimicrobial Activity and Docking Assisted Prediction of the Mechanism of their Antibacterial and Antifungal Properties

2020 ◽  
Vol 20 (29) ◽  
pp. 2681-2691
Author(s):  
Athina Geronikaki ◽  
Victor Kartsev ◽  
Phaedra Eleftheriou ◽  
Anthi Petrou ◽  
Jasmina Glamočlija ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Drug Targets ◽  
Bacterial Species ◽  
Pharmaceutical Research ◽  
Docking Studies ◽  
Fungal Species ◽  
Docking Analysis ◽  
E Coli ◽  
Antibacterial And Antifungal

Background: Although a great number of the targets of antimicrobial therapy have been achieved, it remains among the first fields of pharmaceutical research, mainly because of the development of resistant strains. Docking analysis may be an important tool in the research for the development of more effective agents against specific drug targets or multi-target agents 1-3. Methods: In the present study, based on docking analysis, ten tetrahydrothiazolo[2,3-a]isoindole derivatives were chosen for the evaluation of the antimicrobial activity. Results: All compounds showed antibacterial activity against eight Gram-positive and Gram-negative bacterial species being, in some cases, more potent than ampicillin and streptomycin against all species. The most sensitive bacteria appeared to be S. aureus and En. Cloacae, while M. flavus, E. coli and P. aeruginosa were the most resistant ones. The compounds were also tested for their antifungal activity against eight fungal species. All compounds exhibited good antifungal activity better than reference drugs bifonazole (1.4 – 41 folds) and ketoconazole (1.1 – 406 folds) against all fungal species. In order to elucidate the mechanism of action, docking studies on different antimicrobial targets were performed. Conclusion: According to docking analysis, the antifungal activity can be explained by the inhibition of the CYP51 enzyme for most compounds with a better correlation of the results obtained for the P.v.c. strain (linear regression between estimated binding Energy and log(1/MIC) with R 2 =0.867 and p=0.000091 or R 2 = 0.924, p= 0.000036, when compound 3 is excluded.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

2020 ◽  
pp. 78-84
Author(s):  
FAVIAN BAYAS-MOREJON ◽  
ANGELICA TIGRE ◽  
RIVELINO RAMON ◽  
DANILO YANEZ
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Potassium Phosphate ◽  
Natural Antioxidants ◽  
Butylated Hydroxytoluene ◽  
Penicillin G ◽  
Diffusion Method ◽  
E Coli ◽  
Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

scholarly journals Bioactive Compounds, Antioxidant Activity, and Biological Effects of European Cranberry (Vaccinium oxycoccos)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 24 ◽  
Author(s):  
Tunde Jurikova ◽  
Sona Skrovankova ◽  
Jiri Mlcek ◽  
Stefan Balla ◽  
Lukas Snopek
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Folk Medicine ◽  
Meat Products ◽  
Biologically Active ◽  
Anticancer Activities ◽  
Fruit Species ◽  
American Cranberry ◽  
Antibacterial And Antifungal ◽  
Active Substances

Lesser known fruits or underutilized fruit species are recently of great research interest due to the presence of phytochemicals that manifest many biological effects. European cranberry, Vaccinium oxycoccos fruit, as an important representative of this group, is a valuable source of antioxidants and other biologically active substances, similar to American cranberry (V. macrocarpon) which is well known and studied. European cranberry fruit is rich especially in polyphenolic compounds anthocyanins (12.4–207.3 mg/100 g fw), proanthocyanins (1.5–5.3 mg/100 g fw), and flavonols, especially quercetin (0.52–15.4 mg/100 g fw), which mostly contribute to the antioxidant activity of the fruit. Small cranberry is also important due to its various biological effects such as urinary tract protection (proanthocyanidins), antibacterial and antifungal properties (quercetin, proanthocyanidins, anthocyanins), cardioprotective (proanthocyanidins) and anticancer activities (proanthocyanidins), and utilization in food (juice drinks, jams, jellies, sauces, additive to meat products) and pharmacological industries, and in folk medicine.

scholarly journals COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES OF THE HORSE CHESTNUT (AECULUS HIPPOCASTANUM L.)

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 33-39
Author(s):  
V.P. Kurchenko ◽  
◽  
N.V. Sushinskaya ◽  
K.I. Maiorava ◽  
E.I. Tarun ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Horse Chestnut ◽  
Hazard Class ◽  
Active Substances

The study of the composition of biologically active substances, alcoholic extracts from flowers of Aeculus hippocastanum L. According to the results of HPLC-MS and GC-MS analyzes, the extract contains the main amounts of phenolic compounds: quercetin, epicatechin, kaempferol. In addition, the extract contains fatty acids and their esters, alcohols, 3-deoxy-d-manno lactone, 1,2,3,5-cyclohexantethrol, α-methyl-mannofuranoside, γ sitosterol. Antioxidant activity of an extract from flowers of A. hippocastanum is associated with the peculiarities of the compositionof biologically active substances. The toxological-hygienic assessment of flowers of this species in acute and subacute experiments showed that it belongs to the 4th hazard class (low hazard).

scholarly journals ЭКОЛОГО-ФИТОХИМИЧЕСКИЕ ОСОБЕННОСТИ ЦВЕТКОВ БУЗИНЫ ЧЕРНОЙ SAMBUCUS NIGRA L. В УСЛОВИЯХ УРБАНИЗИРОВАННОЙ СРЕДЫ

2021 ◽  
Vol 13 (4) ◽  
pp. 1
Author(s):  
Н.А. Виноградова
Keyword(s):  
Antioxidant Activity ◽  
Biologically Active ◽  
Sambucus Nigra ◽  
Lead And Cadmium ◽  
Total Antioxidant ◽  
Phenolic Substances ◽  
The Russian Federation ◽  
Herbal Medicinal ◽  
Urbanized Areas ◽  
Active Substances

The impacts of the urbanized environment of Donetsk region on the contents of biologically active substances and heavy metals in black elderberry (Sambucus nigra L.) flowers and on the total antioxidant activity of their preparations were evaluated. The contents of lead, cadmium and mercury in all samples studied were within the tolerable limits specified for herbal medicinal raw products. The accumulation coefficients of lead and cadmium were found to decrease with increasing soil levels of these toxicants, whereas the level of mercury did not depend on the degree of soil pollution. The contents of active substances in all samples collected in Donbas conform to the requirements of the State Pharmacopoeia of the Russian Federation. In fact, the contents of carotenoids, flavonoids and hydroxycinnamic acids are increased in S. nigra flowers collected in urbanized areas; however, the contents of other phenolic substances (anthocyanins and tannins) are decreased. The contents of ascorbic and free organic acids are highest under moderately urbanized conditions, so as the antioxidant activity of S. nigra flower preparations is. The results suggest that prospects for the pharmaceutical use of S. nigra flowers collected in urbanized areas of Donbas are promising.

scholarly journals Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2669
Author(s):  
Michaela Barkociová ◽  
Jaroslav Tóth ◽  
Katarzyna Sutor ◽  
Natalia Drobnicka ◽  
Slawomir Wybraniec ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Food Industry ◽  
Biological Activities ◽  
Ornamental Plants ◽  
Reducing Power ◽  
Activity Assay ◽  
Natural Pigment ◽  
E Coli ◽  
Ft Ir

Epiphyllum, Hylocereus, and Opuntia plants belong to the Cactaceae family. They are mostly known as ornamental plants but also for their edible fruits, which can potentially be sources of betalains, such as betanin, a natural pigment used in the food industry, e.g., under the European label code E 162. The aim of this work was the identification of betalains (using LC-MS/MS), evaluation of total betalain content (spectrophotometrically), analysis of functional groups (using FT-IR), evaluation of antioxidant activity (using DPPH, ABTS, FRAP, DCFH-DA, and reducing power methods) and evaluation of antimicrobial activity (S. aureus, E. coli, and C. albicans) in fruits of Epiphyllum, Hylocereus, and Opuntia taxa. A total of 20 betalains were identified in the studied Cactaceae fruits. The Epiphyllum pink hybrid had the highest values of total betalains amongst all samples. The highest antioxidant activity was observed in the Epiphyllum pink hybrid, in Opuntia zacuapanensis and O. humifusa fruits. The antimicrobial activity assay showed that cacti fruits were not able to effectively inhibit the growth of E. coli, S. aureus, or C. albicans. Our results prove that these fruits are good sources of natural pigments—betalains. They do not contain toxic compounds in significant amounts and they exhibit antioxidant activity.

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals Development of beverage recipes with specified bio-corrective properties based on plant raw materials with the ultrasonic extraction method

2020 ◽  
Vol 82 (3) ◽  
pp. 189-199
Author(s):  
M. V. Manukovskaya ◽  
I. P. Shchetilina ◽  
A. G. Kudryakova ◽  
S. R. Kozlova ◽  
A. O. Torosyan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Human Body ◽  
Extraction Method ◽  
Raw Materials ◽  
Ultrasonic Extraction ◽  
Biologically Active ◽  
Plant Raw Materials ◽  
Organoleptic Characteristics ◽  
Active Substances

The development of targeted beverages is a priority for healthy eating. Plant raw materials containing a wide variety of biologically active substances are used for their production. The presence of these ingredients helps to improve many physiological processes in the body, as well as to increase its immune status. The high efficiency of ultrasonic effects on various technological processes was confirmed by numerous studies. Ultrasonic extraction was carried out at t = 20 ? and an ultrasonic wave frequency of 22 kHz. As a result of the research, it was found that the equilibrium state is achieved within 15 minutes with ultrasound exposure. Natural raw materials, their chemical composition and the influence on the human body were studied in the work to develop new beverages recipes. The selected ingredients in a certain quantitative ratio made it possible to obtain beverages with improved bio-corrective properties, which allows satisfying the daily requirement of the human body for biologically active substances. Ultrasonic exposure has a positive effect on the organoleptic characteristics of the beverage and the aftertaste, as proved by the comparative organoleptic evaluation of the samples. The functional properties of the beverages developed were confirmed experimentally. The following was determined in the beverages: macronutrients content, antioxidant activity, vitamin C content, shelf life duration. The ultrasonic extraction method application in the technology of beverages manufacturing allows: to reduce the preparation time by half, to reduce the amount of raw materials by 15%, to increase the content of macronutrients by 45%, to increase the content of vitamin C by 35%, to increase the antioxidant activity by 45-50%, to increase storage stability by 50-60%, to improve organoleptic characteristics. The results obtained prove the intensifying effect of ultrasonic exposure in the production of functional beverages.

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