Protein metabolism, physicochemical properties and chemical composition of muscle tissue in Large White weaners

We presented the blood serum biochemical parameters, physicochemical properties and chemical composition of muscle tissue in Large White weaners of British origin. We found that serum biochemical parameters in five months weaners were within the physiological norm (the content of total protein was 71.28 g/l (?V = 8.16%), creatinine concentration was 155.62 µmol L-1 (?V = 14.13%). Studies of physicochemical properties and chemical composition of samples of Longissinus dorsi muscle of Large White young pigs show that the number of high quality samples in terms of moisture holding capacity, % is 12.0%, colour intensity, units of ext.×1000 – 16.0%, tenderness, s – 12.0% and fat content, % – 16.0%. We revealed significant correlations between moisture holding capacity and protein content (r = -0.484, tr = 2.65), moisture holding capacity and hygroscopic moisture content (r = 0.402, tr = 2.11). The correlation between total protein content in blood serum and fat content in muscle tissue was -0.257 (tr = 1.28), whereas the correlation between serum total protein content and calcium content in muscle tissue was 0.375 (tr = 1.94).

Download Full-text

Age – Related changes in serum biochemical parameters in the domestic rabbit (Oryctolagus cuniculus)

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i5.211 ◽

2020 ◽

Vol 45 (5) ◽

pp. 67-71

Author(s):

K. A. Adebisi

Keyword(s):

Total Protein ◽

Biochemical Parameters ◽

Serum Biochemistry ◽

Serum Biochemical Parameters ◽

Serum Total Protein ◽

Domestic Rabbit ◽

Age Related ◽

Protein 4.2 ◽

Pubertal Stages ◽

Serum Biochemical

The influence of age on serum biochemical components in the domestic rabbit was investigated. The serum total protein (g/dL), albumin (g/dL), globulin (g/dL), creatinine (mg/dL), urea mg/dL, cholesterol (mg/dL), Alkaline phosphatase (ALP; iu/l), Aspartate aminotranferase (AST; iu/l) and Alanine amimotransferase (ALT; iu/l) were assessed in 24 male rabbits at 7, 14 and 21 weeks of age to represent weaners, growers and pubertal stages. Data were analysed using descriptive statistics and analysis of variance at á . Results of 0.05 serum biochemistry revealed a steady significant increase in total protein (4.2±0.3; 5.3±0.5; 6.9±0.2), albumin (2.2±0.7; 2.8±0.4; 4.0±0.2), globulin (1.9±0.3; 2.5±0.4, 2.9±0.2), cholesterol (49.5±1.1; 56.4±4.2; 62.4±5.4) and creatinine (0.8±0.1; 1.2±0.1; 1.9±0.3) at 7, 14 and 21 weeks respectively. Serum urea levels were similar across the ages. The values recorded for ALP and AST at 7 weeks (39.6±7.5; 101.1±6.7) and 14 weeks (38.7±6.4; 104.5±8.7) were significantly higher than the 21 weeks (33.5±2.7; 95.8±5.9) while ALT at 7 weeks (27.33±5.6) was significantly lower than 14 (38.2±3.9) and 21 weeks (37.4±3.0). The age of the rabbits had a significant effect on their serum biochemical parameters. They should therefore be grouped according to age when setting serum reference values for them.

Download Full-text

Influence of salicylate administration on weight gain and serum biochemical parameters in broiler chickens

Medycyna Weterynaryjna ◽

10.21521/mw.432 ◽

2010 ◽

Vol 66 (10) ◽

pp. 675-679 ◽

Cited By ~ 2

Author(s):

Poźniak B. ◽

Yuan H. ◽

J. Yi ◽

Guo C. ◽

Deng S. ◽

...

Keyword(s):

Body Weight ◽

Total Protein ◽

Biochemical Parameters ◽

Broiler Chickens ◽

Clinical Manifestations ◽

Weight Ratio ◽

Serum Biochemical Parameters ◽

Serum Total Protein ◽

Weight Gains ◽

Serum Biochemical

The aim of the study was to investigate the effects of different doses of acetylsalicylic acid (ASA) and sodium salicylate (SS) administration on weight gains and serum biochemical parameters in broiler chickens. This study was performed to investigate the safety of ASA and SS in chickens in conditions of moderate overdose. Seventy five broiler chickens (Sanhuangji breed) were divided into control and experimental groups. The chickens were administered orally with ASA or SS in the following daily doses: 50, 100 and 200 mg/kg for 20 days. In the course of the experiment weight gains were recorded. On day 14 and day 20 the chickens were sampled for blood biochemical analysis. Investigated parameters included serum total protein, albumin, uric acid, creatinine, potassium, sodium and calcium contents as well as alanine transaminase activity. After the last samplings the chickens were dissected and liver and kidney to body weight ratios were recorded. It was demonstrated that SS at a dose of 200 mg/kg increased serum total protein level. ASA increased serum proteins at a dose of 100 mg/kg but not at a higher dose. At the dose of 200 mg/kg ASA increased the kidney to body weight ratio. In all salicylate treated groups the liver weights were lower as compared to the control. No clinical manifestations of intolerance were observed. It is assumed that chickens tolerate ASA and SS well, even in doses producing distinct side effects in several mammalian species.

Download Full-text

The effect of camel milk curd masses on rats blood serum biochemical parameters: Preliminary study

PLoS ONE ◽

10.1371/journal.pone.0256661 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0256661

Author(s):

Fatima Dikhanbayeva ◽

Elmira Zhaxybayeva ◽

Zhuldyz Smailova ◽

Arman Issimov ◽

Zhechko Dimitrov ◽

...

Keyword(s):

Blood Serum ◽

Total Protein ◽

Biochemical Parameters ◽

Camel Milk ◽

Control Group ◽

Serum Biochemical Parameters ◽

Group V ◽

Group I ◽

Serum Biochemical ◽

Group Ii

This study aimed to assess potential feeding effect of camel milk curd mass and its mixes to experimental rat’s blood serum biochemical parameters, enzymatic activity and the peptide toxicity. Fifty healthy male Sprague-Dawley rats were divided into five groups (n = 10 each). Each group was fed with camel milk pure curd mass and its mixes for 16 days. At the end of the experiment, rats were sacrificed to collect the samples from the blood serum. Blood serum biochemical parameters total protein, cholesterol, glucose, albumin, triglycerides; the enzymatic activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were determined on the A25 automatic analyser, and peptide toxicity analysed by the reference method. The statistical data have shown no significant differences in body weight gain in all groups. Total protein decreased in group II, IV, and V; however, it increased in group III compared to the control group. Cholesterol grew up in group II and it slightly increased in group V, dropped in groups III and IV compared to group I result. Glucose increased in groups II, III, IV compared to group I; still, group V results show a slight decrease. Albumin decreased in group IV, yet in group V it increased than the group I result. Simultaneously, groups II and III results were changed with less percentage. Triglyceride grew up in groups II, V, and it dropped significantly in groups III, IV compared to the control group. De Ritis ratio of enzymes in groups II, III, and IV fluctuated between 1.31 and 0.98 IU/L; however, group V demonstrated significant data versus group I. Diets peptide toxicity in all groups was lower than control group data. The experimental results indicated that curd mass from camel milk could be used as a pure or with additives and it did not discover the observed side effects.

Download Full-text

Chemical characterization of Brazilian hulless barley varieties, flour fractionation, and protein concentration

Scientia Agricola ◽

10.1590/s0103-90162004000600005 ◽

2004 ◽

Vol 61 (6) ◽

pp. 593-597 ◽

Cited By ~ 10

Author(s):

Cristiane Vieira Helm ◽

Alicia de Francisco

Keyword(s):

Chemical Composition ◽

Protein Content ◽

Total Protein ◽

Protein Concentration ◽

Chemical Characterization ◽

Total Protein Content ◽

Human Consumption ◽

Published Data ◽

Protein Nitrogen ◽

Hulless Barley

Even though hulless barley is widely known due to its nutritional potential, in Brazil it is only grown at a few agricultural experimental stations. There is no published data about the chemical composition of Brazilian hulless barley varieties; however, research laboratories have studied their agronomical characteristics. The objectives of this study were to present the chemical characterization and effect of flour fractionation on protein concentration of six Brazilian hulless barley varieties, namely IAC IBON 214/82, IAC 8612/421, IAC 8501/31, IAC 8501/12, IAPAR 39-Acumaí, and IAC 8501/22. The analyses included: ash, ether extract, total protein, starch, total insoluble and soluble dietary fiber, and beta-glucans. Flour fractionation was carried out by sieving. The flour fractions were evaluated for crude protein, protein, and protein and non-protein nitrogen. Chemical composition varied (P < 0.05) among all the varieties. IAC 8501/22, IAC 8501/31, and IAC 8501/12 showed the highest protein content (15.69, 15.25, and 14.94% respectively). Differences (P < 0.05) among the protein of the fractionated flours were detected, and might be attributed primarily to genetic background since all varieties were grown under the same environmental conditions. Fractionating the flour increased the total protein content, in some fractions, by up to 2%. These results may be useful in the food industry for the selection of hulless barley varieties for human consumption and to produce substantially protein-enriched flour fractions.

Download Full-text

Beebread from Apis mellifera and Apis dorsata. Comparative Chemical Composition and Bioactivity

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:12620 ◽

2017 ◽

Vol 74 (1) ◽

pp. 43

Author(s):

Otilia BOBIS ◽

Daniel Severus DEZMIREAN ◽

Liviu Alexandru MARGHITAS ◽

Victorita BONTA ◽

Rodica MARGAOAN ◽

...

Keyword(s):

Apis Mellifera ◽

Chemical Composition ◽

Protein Content ◽

Total Protein ◽

Soxhlet Extraction ◽

Climatic Conditions ◽

Total Protein Content ◽

Composition Analysis ◽

Apis Dorsata ◽

Bioactive Properties

Beebread is a valuable bee product, both for bee nutrition and for humans. The high nutritional and bioactive properties of beebread were evaluated by chemical composition analysis of beebread from Apis mellifera and Apis dorsata. Bee bread harvested from Romania and India, coming from Apis mellifera and Apis dorsata bees, were evaluated for their chemical composition. Analyses were made in APHIS Laboratory from USAMV Cluj, using validated methods for bee products. Lipids were determined by the Soxhlet extraction method, total protein content was determined by Kjehldahl method, sugar spectrum was determined by high performance liquid chromatography with refractive index detection (HPLC-IR). Water content of beebread samples were situated between 11.45 and 16.46%, total protein content between 16.84 and 19.19% and total lipids between 6.36 and 13.47%.Â Beebread has high bioactive properties which can be expressed as antioxidant and/or antibacterial activity. Chemical composition and bioactive properties of beebread is influenced by floral origin of the pollen which the bees collect and place in combs for fermentation. Also the climatic conditions have an important role in developing different fermentation compounds, that may act as antioxidants or antibacterial agents.

Download Full-text

The effect of increased selenium and vitamin E in the feeding of fattening pigs on their growth, chemical composition of meat and serum biochemical parameters

Acta Scientiarum Polonorum Zootechnica ◽

10.21005/asp.2020.19.4.05 ◽

2021 ◽

Vol 19 (4) ◽

pp. 39-46

Author(s):

Anita Kołodziej-Skalska ◽

◽

Arkadiusz Pietruszka ◽

Beata Matysiak ◽

◽

...

Keyword(s):

Vitamin E ◽

Chemical Composition ◽

Biochemical Parameters ◽

Serum Biochemical Parameters ◽

Serum Biochemical ◽

Fattening Pigs

Celem pracy była ocena wpływu uzupełniania standardowych mieszanek paszowych dla tuczników dodatkowymi ilościami selenu i witaminy E na wyniki tuczu, skład chemiczny mięsa i parametry biochemiczne surowicy tuczników. Badania przeprowadzono na 60 tucznikach linii 990. Zwierzęta przydzielono do 4 grup: grupa kontrolna (CG) otrzymująca mieszankę pełnoporcjową zawierającą 0,3 mg Se (Na2SeO3) i 60 mg witaminy E · kg–1; grupa SE - otrzymująca dodatek 0,2 mg organicznego Se · kg–1; grupa VE - otrzymująca 60 mg witaminy E · kg–1; oraz grupa VE + SE - otrzymująca dodatkowo 60 mg witaminy E · kg–1 i 0,2 mg organicznej Se · kg–1. Badania wykazały, iż żywienie tuczników zwiększoną ilością selenu i witaminy E, może nieznacznie pogorszyć wykorzystanie paszy i mięsność, ale obniża zawartość cholesterolu w surowicy oraz mięsie tuczników.

Download Full-text

Effects of xylene and formaldehyde inhalations on renal oxidative stress and some serum biochemical parameters in rats

Toxicology and Industrial Health ◽

10.1177/0748233707078218 ◽

2007 ◽

Vol 23 (2) ◽

pp. 115-120 ◽

Cited By ~ 17

Author(s):

Cavit Kum ◽

Selim Sekkin ◽

Funda Kiral ◽

Ferda Akar

Keyword(s):

Oxidative Stress ◽

Total Protein ◽

Biochemical Parameters ◽

Control Group ◽

Serum Biochemical Parameters ◽

Sprague Dawley ◽

Group 4 ◽

Sprague Dawley Rats ◽

Serum Biochemical ◽

Group 2

In this study, it was aimed to demonstrate the possible renal oxidative stress and some serum biochemical parameters and their alterations caused by the exposure to xylene and formaldehyde (HCHO) in rats. Weighing 150—200 g, 12-week-old, 24 female Sprague-Dawley rats were used. The rats were randomly divided into four groups: Group 1 (control), Group 2 (300-ppm technical xylene), Group 3 (6-ppm HCHO) and Group 4 (150-ppm technical xylene + 3-ppm HCHO). The animals were exposed to gases eight hours per day for six weeks. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, glutathione (GSH) and malondialdehyde (MDA) levels were measured. In addition, serum total protein, albumin, urea and creatinine levels were evaluated. Compared with the control animals, urea levels increased significantly in all groups ( P < 0.001). GSH activities and MDA levels increased in xylene and xylene + HCHO groups ( P < 0.05). No statistically considerable differences were found in SOD, CAT and GSH-Px activities, total protein, albumin and creatinine levels among all groups ( P > 0.05). The present study indicates but not statistically confirms the renal toxicity of the exposures to xylene, HCHO and a mixture of them.

Download Full-text