scholarly journals ПОРІВНЯЛЬНА ОЦІНКА ДІЇ ДЕЗІНФІКУЮЧИХ ЗАСОБІВ З МИЮЧИМ ЕФЕКТОМ ПРИ ЗНЕЗАРАЖЕННІ E. СOLI ТА S. АUREUS

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 88-92
Author(s):  
V.L. Kovalenko ◽  
A.V. Zagrebelnyi ◽  
O.I. Vishchur ◽  
A.I. Chekhun
Keyword(s):  
Benzalkonium Chloride ◽  
Cotton Swab ◽  
Minimum Effective Concentration ◽  
E Coli ◽  
Initial Suspension ◽  
Test Organisms ◽  
And Control ◽  
Test Objects ◽  
Sterile Cotton Swab

A comparative analysis of the use detergents disinfectants with different active ingredients: heotsyd (polihexametylenhuanidin hydrochloride, benzalkonium chloride) virosan (alkildymetylbenzylamoniya chloride, glutaraldehyde) neohlor (sodium hypochlorite) orhasept (benzalkonium chloride, lactic acid) and determination of their minimum effective concentration and exposure required for removal of E. coli and S. aureus on test sites: stainless steel, tile, concrete, brick was were conducted. At sterilized in an autoclave test objects applied to 1 cm3 culture E. coli and S. aureus at a concentration of 2 billion. mikr.til per cm3. Contaminated test objects dried and placed into cuvettes horizontally and vertically and spray disinfectants applied explored solutions, while noting their exposure concentration and amount spent. Objects whish were exposure  by the same scheme, but with using sterile water were used as control. After a certain period of time by sterile cotton swab from experimental and control test objects were taken probes. Then 1 cm3 initial suspension of each of these tubes were placed in the appropriate environment for 24 hours. at thermostat at 37 °C. By the changes in defined media presence or absence of these microorganisms were determined.Stated that disinfectants orhasept and virosan in 0.1% concentration and heotsyd neohlor in 0.5% concentration completely disinfected from E. coli and S. aureus on surfaces of the test objects for 10 min, confirms their detergent disinfectant effect. Effective concentrations of disinfectants regarding test organisms consistent with regulations for their use.

scholarly journals Features of the determination of drinking water toxicity

2016 ◽  
pp. 50-55
Author(s):  
Е. Аrystarkhova
Keyword(s):  
Drinking Water ◽  
Chronic Effect ◽  
Water Toxicity ◽  
Quality Testing ◽  
Influence Of Water ◽  
Test Organisms ◽  
Tradescantia Fluminensis ◽  
Living Organisms ◽  
Test Objects

The article is devoted to the problem of the determination of drinking water toxicity in the system of water supply of Zhytomyr сity. An increase of reliability of control of drinking water quality is especially important in the conditions of tense ecological situation. That’s why it is expediently to apply so-called sets of test-organisms in which the representatives of vegetable and animal forms have to become as obligatory components for determination of water ecotoxicological potential. The results of such a biological testing enable more fully to estimate influence of contaminents on living creatures. It was suggested during the leadthrough of quality testing of drinking water to use Daphnia magna Straus and Tradescantia fluminensis Vellozo in the presented researches. On the basis of test-objects reactions the index of drinking water toxicity was calculated. The harmful influence of water secondary pollution on the living organisms (index of toxicity 50%, group D1) is noted. It was found out the specificity of  sensitiveness of tradescantia (on 8th day) and daphnia (on 15th day) to chronic effect of drinking water components.

scholarly journals Antibacterial effect of Aqueous Neem (Azadirachta indica) leaf extract, crude neem leaf paste, and Ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

2016 ◽  
Vol 3 (1) ◽  
pp. 25-36
Author(s):  
Shamima Sultana ◽  
A. S. M. Shahidullah ◽  
Md. Mahbubul Islam ◽  
A. F. S. A. Wasey ◽  
Shamsun Nahar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Leaf Extract ◽  
Inhibitory Effects ◽  
Dilution Technique ◽  
E Coli ◽  
Test Organisms ◽  
Broth Dilution

The study was conducted during the period of July 2004 to June 2005 in the   Department of Pharmacology & Therapeutics in collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh to determine the profile of antibacterial effect of crude neem leaf paste (CNLP), aqueous neem leaf extract (ANLE), and standard antibiotic Ceftriaxone against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. Five separate experiments were done e.g. I) Determination of inhibitory effect of crude neem leaf paste (CNLP) by incorporation into nutrient agar media (NA), against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. II) Determination of minimum inhibitory concentration (MIC) of aqueous neem leaf extract (ANLE) against that three test organisms by broth dilution technique, III) Determination of minimum inhibitory concentration (MIC) of standard antibiotic ceftriaxcone against test organisms by broth dilution technique as well as making a comparison with MIC of ANLE and IV) Subculture study of materials from effective CNLP, ANLE, NLEE and Ceftriaxone in nutrient agar medium for confirmation of respective results of different experiments conducted. Results revealed that inhibitory effects were observed against the growth of Staph. aureus, Esch. coli and Ps. aeruginosa at 15%, 20% and 25% respectively of CNLP incorporated  into NA media.  The broth dilution technique was followed to determine the MICs of ANLE and Ceftriaxone. The MIC of ANLE was 714 μg/ml against S. aureus and that against E. coli and P. aeruginosa was 1428 μg/ml. The MIC of Ceftriaxone was 10μg/ml against S. aureus and that against E. coli and P. aeruginosa was 25 μg/ml. The MIC of Ceftriaxone was the lowest in comparison to MICs of ANLE. The subculture study showed similar results with that of previous experiments in terms of inhibitory effects of CNLP and MICs of ANLE, Ceftriaxone against all of the organisms studied.  

STUDY OF DISINFECTING EFFICIENCY OF «PALOCID» FOR DISINFECTION OF OBJECTS OF VETERINARY SUPERVISION

2018 ◽  
Vol 1 (1) ◽  
pp. 44-49
Author(s):  
N. I. Popov ◽  
◽  
S. A. Michko ◽  
S. M. Lobanov ◽  
Z. E. Alieva ◽  
...  
Keyword(s):  
Coli Strain ◽  
Aureus Strain ◽  
Railway Transport ◽  
Viral Etiology ◽  
E Coli ◽  
Test Culture ◽  
Natural Contamination ◽  
And Control ◽  
Test Objects

The results of laboratory and production tests of the effectiveness of the disinfectant Palocid for the disinfection of objects of veterinary supervision are presented. As test microorganisms were used the museum cultures of E. coli (strain 1257), Staphylococcus aureus (strain 209-P), bacilli (strain B5), B. cereus (strain 96). Inactivated blood serum of the horse was used to simulate natural contamination of surfaces. The quality of the disinfection was controlled by examining the washings from the test and control test objects for the presence of a given test culture. The results of laboratory and industrial tests show that Palocid is an effective disinfectant and can be recommended for preventive and forced disinfection in livestock, poultry, fur farming, automobile and railway transport, while monitoring its quality in the isolation of bacteria of the E. coli and staphylococcus group, as well as forced disinfection at the objects of veterinary supervision for infectious diseases of bacterial (including tuberculosis) and viral etiology, especially dangerous infections (anthrax and other spore infections).

JUSTIFICATION OF DESTRUCTION MODES OF ORGANIC LIVESTOCK AT THERMAL DRYING IN VACUUM

2019 ◽  
Vol 1 (1) ◽  
pp. 68-73
Author(s):  
V. G. Tyrin ◽  
◽  
G. A. Mysova ◽  
K. N. Biryukov ◽  
N. N. Potemkina ◽  
...  
Keyword(s):  
Thermal Processing ◽  
Organic Waste ◽  
Organic Substrate ◽  
Animal Waste ◽  
Thermal Method ◽  
E Coli ◽  
Drying Chamber ◽  
Test Objects ◽  
Test Cultures

The article scientifically substantiates the modes of disinfection of organic waste on the basis of litter in the thermal method of their processing in vacuum. Experiments on the determination of the modes of disinfection of organic animal waste during their thermal processing method were carried out in a drying chamber Vacuum EcoDry loaded with prepared initial organic substrate into which test objects contaminated with suspensions of various groups of microorganisms: E. coli, S. aureus – 209 P, an atypical strain of mycobacteria B-5 and B. cereus. The results were evaluated by the survival of the test cultures after vacuum heat treatment (drying) of organic waste. Disinfection of organic animal waste during the technological process of vacuum drying is achieved at an installation temperature of 75 °C and more, at humidity of 75-80 mm Hg, an exposure of at least 50 minutes and allows you to get safe in the sanitary relation of the product of their processing.

Ultrastructural changes in sciatic nerve tissue: Effects of passive maternal smoking

1983 ◽  
Vol 41 ◽  
pp. 650-651
Author(s):  
Amankwah K.S. ◽  
A.D. Weberg ◽  
R.C. Kaufmann
Keyword(s):  
Sciatic Nerve ◽  
Maternal Smoking ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Nerve Tissue ◽  
Ultrastructural Changes ◽  
Cacodylate Buffer ◽  
And Control ◽  
Spontaneous Delivery

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.

Concentration determination of chromatin in unstained resin-embedded sections by means of Z-contrast in STEM

1990 ◽  
Vol 48 (2) ◽  
pp. 186-187
Author(s):  
M. Haider ◽  
B. Bohrmann
Keyword(s):  
Energy Loss ◽  
Freeze Substitution ◽  
Biological Macromolecules ◽  
Local Concentration ◽  
E Coli ◽  
Concentration Determination ◽  
Phosphorous Content ◽  
Z Contrast ◽  
Electron Energy Loss

The technique of Z-contrast in STEM offers the possibility to determine the local concentration of macromolecules like lipids, proteins or DNA. Contrast formation depends on the atomic composition of the particular structure. In the case of DNA, its phosphorous content discriminates it from other biological macromolecules. In our studies, sections of E. coli, the dinoflagellate Amphidinium carterae and Euglena spec. cells were used which were obtained by cryofixation followed by freeze-substitution into acetone with 3% glutaraldehyde. The samples were then embedded either in Lowicryl HM20 at low temperature or in Epon at high temperature. Sections were coated on both sides with 30Å carbon.The DF- and the inelastic image have been recorded simultaneously with a Cryo-STEM. This Cryo-STEM is equipped with a highly dispersive Electron Energy Loss Spectrometer. With this instrument pure Z-contrast can be achieved either with a Filtered DF-image divided by the inelastic image or, as is used in this paper, by dividing the conventional DF-image by an inelastic image which has been recorded with an inelastic detector whose response is dependent on the total energy loss of the inelastically scattered electrons.

A Study of the Efficiency of Modern Domestic Disinfectants in the System of TB Control Activities

2015 ◽  
Vol 2 (2) ◽  
pp. 26-31 ◽  
Author(s):  
A. Paliy ◽  
A. Zavgorodniy ◽  
B. Stegniy ◽  
A. Gerilovych
Keyword(s):  
Molecular Genetic ◽  
Critical Role ◽  
Bactericidal Effect ◽  
Chain Reaction ◽  
Tb Control ◽  
Source Of Infection ◽  
Polymerase Chain ◽  
And Control ◽  
Chemical Groups ◽  
Test Objects

Due to the absence of elaborated effi cient means for specifi c prevention of bovine tuberculosis, it is ex- tremely important to detect and eliminate the source of infection and to take veterinary and sanitary preven- tive measures. Here the critical role is attributed to disinfection, which breaks the epizootic chain due to the elimination of pathogenic microorganisms in the environment and involves the application of disinfectants of different chemical groups. Aim. To study the tuberculocidal properties of new disinfectants DZPT-2 and FAG against atypical mycobacteria Mycobacterium fortitum and a TB agent Mycobacterium bovis. Methods. The bacteriological and molecular-genetic methods were used. Results. It was determined that DZPT-2 prepara- tion has bactericidal effect on M. fortuitum when used in the concentration of 2.0 % of the active ingredient (AI) when exposed for 5–24 h, while disinfectant FAG has a bactericidal effect in the concentration of 2.0 % when exposed for 24 h. Disinfectant DZPT-2 in the concentration of 2.0 % of the AI, when exposed for 5–24 h, and FAG preparation in the concentration of 2.0 %, when exposed for 24 h, and with the norm of consump- tion rate of 1 cubic decimeter per 1 square meter disinfect the test-objects (batiste, wood, glazed tile, metal, glass), contaminated with the TB agent M. bovis. Conclusions. Disinfecting preparations of DZPT-2 in the concentration of 2.0 % of AI when exposed for 5 h and FAG in the concentration of 2.0 % when exposed for 24 h may be used in the complex of veterinary and sanitary measures to prevent and control TB of farm ani- mals. The possibility of using the polymerase chain reaction as an additional method of estimating tuberculo- cide activity of disinfectants was proven.

Co-expression of recombinant single chain variable fragment recognizing blood antigen fused with sumo and chaperones in Escherichia coli

2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Dang Thi Ngoc Ha ◽  
Le Thi Thu Hong ◽  
Truong Nam Hai
Keyword(s):  
Recombinant Antibody ◽  
Blood Type ◽  
Soluble Form ◽  
Supernatant Fraction ◽  
Blood Group Antigens ◽  
Single Chain ◽  
E Coli ◽  
Recombinant Scfv ◽  
Single Chain Variable Fragments

Single chain variable fragments (scFv) have widely been used in research, diagnosis and treatment, but the scFv is considered as difficult protein for expression in E. coli. In previous studies, we expressed a construction of recombinant single chain variable fragments again antigen specific for blood type A (antiA-scFv) individually or fused with Trx or SUMO. However, soluble fraction was low abandant and only approximately 40% when fused with Trx, the other cases were expressed in form of inclusion body. Therefore, it was difficult for purification, refolding and activity assesment. In thispaper, we demonstrated a suitable construction for soluble production of antiA-scFv fused with SUMO (SM/antiA-scFv) in presence of chaparones. Under fermentation with 0.1 mM IPTG at 20oC, the SM/antiA-scFv was entirely expressed in soluble form. Importantly, after cleavage from SUMO with SUMOprotease, antiA-scFv was still maintained in the supernatant fraction. Therefore, it can help ensure bioactivity and is useful for purification process. To the best of our knowledge, this is the first report showing soluble recombinant scFv fused with SUMO in presence of chaperone for determination of blood group antigens. Thus, this result facilitates the optimal study of soluble expression, purification and bioactivity determination of the antiA-scFv recombinant antibody. 

Application of EDS Technique for OSP Film Thickness Measurement

2006 ◽  
Author(s):  
Prong Kongsubto ◽  
Sirarat Kongwudthiti
Keyword(s):  
Thin Film ◽  
Quantitative Analysis ◽  
Joint Strength ◽  
Critical Factor ◽  
Thickness Measurement ◽  
Ic Manufacturing ◽  
And Control ◽  
Film Thickness Measurement ◽  
Non Destructive

Abstract Organic solderability preservatives (OSPs) pad is one of the pad finishing technologies where Cu pad is coated with a thin film of an organic material to protect Cu from oxidation during storage and many processes in IC manufacturing. Thickness of OSP film is a critical factor that we have to consider and control in order to achieve desirable joint strength. Until now, no non-destructive technique has been proposed to measure OSP thickness on substrate. This paper reports about the development of EDS technique for estimating OSP thickness, starting with determination of the EDS parameter followed by establishing the correlation between C/Cu ratio and OSP thickness and, finally, evaluating the accuracy of the EDS technique for OSP thickness measurement. EDS quantitative analysis was proved that it can be utilized for OSP thickness estimation.

Sign in / Sign up

Export Citation Format

Share Document