scholarly journals КОРОЗІЙНА ДІЯ МИЙНО–ДЕЗІНФІКУЮЧОГО ЗАСОБУ «САН–АКТИВ» НА МЕТАЛЕВІ ПОВЕРХНІ ТЕХНОЛОГІЧНОГО ОБЛАДНАННЯ М’ЯСОПЕРЕРОБНОЇ ГАЛУЗІ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 157-162
Author(s):  
V.Z. Salata
Keyword(s):  
Stainless Steel ◽  
Corrosion Rate ◽  
Bactericidal Effect ◽  
Process Equipment ◽  
Meat Production ◽  
Meat Processing ◽  
E Coli ◽  
Corrosive Action ◽  
Processing Plants ◽  
Definition Of

It was developed detergent–sanitizer «San active» for the sanitization of technology equipment for meat processing plants. Active substance of detergent–disinfectant «San active» is the TIME, STEAM, alkali, complexones and corrosion inhibitors. The importance of product development «San active» is that it combines two important features – a washing and disinfection.Previously it was established that means «San active» has good washing properties and shows high bactericidal effect on gram–positive and gram–negative microflora. Bactericidal action of «San active» at 14,8 times is more active compared with phenol, and in the presence in the environment the action of protein substances its activity decreases in 2,3 – 2,4 times. Remedy of 0,5% concentration has bactericidal effect on cells of S. aureus and E. faecalis, which are in the biofilm after 10 minutes of exposure. For the inactivation of cells E. coli and P. aeruginosa, which are in the biofilm, it is necessary that «San active» acted in a concentration not lower than 0,5% and less than 30 minutes. It asw established that the test culture of E. coli and S. aureus are not adapted to solutions of means «San active». A definition of corrosive action of detergent–disinfectant «San active» was designed for sanitary processing of technological equipment at meat processing industry. The degree of corrosion activity was determined by examining the corrosive action of solutions on medium plate, stainless steel, aluminum and galvanized iron, ie metal of construction for process equipment in meat production. It was established that working solutions of 1,0 – 2,0% detergent–disinfectant «San active» are practically not corrosive on stainless steel, because the corrosion was at times less than 200 –66 and 120 – 37 corrosion rate in times less than the acceptable norm. The rate of corrosion of galvanized iron under the influence of 1,0 – 2,0% solution of means «San active» ranged from 0,27 to 0,49 mg/m2 h, that in 2,0 – 5,4 times is higher compared to the effect on stainless steel and 44 – 24 times lower than normal. The rate of corrosion of similar detergent–disinfectants Hlorantoin, Virosan and Bioshaum to galvanized iron ranged from 0,44 to 0,49 mg/m2 h. This indicates the fact that the research variant of means «San active» for the magnitude and speed of corrosion on galvanized iron is not inferior to existing in Ukraine means. The corrosion value of aluminum for the actions of 1,0 – 2,0% solution of means «San active» was 2,2–3,7 times smaller compared to the norm, which refers to the aluminum and corrosion rate was to 1,0% solution of 2,86 mg/m2 h and 2,0% – 4,75 mg/m2 h that in 4,1 – 2,5 times less the standard value.

scholarly journals Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Sapna Chitlapilly Dass ◽  
Joseph M. Bosilevac ◽  
Maggie Weinroth ◽  
Christian G. Elowsky ◽  
You Zhou ◽  
...  
Keyword(s):  
Microbial Community ◽  
Foodborne Pathogens ◽  
Biofilm Formation ◽  
Bacterial Species ◽  
Individual Species ◽  
Meat Processing ◽  
Interspecies Interactions ◽  
E Coli ◽  
Pathogen Survival ◽  
Processing Plants

Abstract Biofilm formation by foodborne pathogens is a serious threat to food safety and public health. Meat processing plants may harbor various microorganisms and occasional foodborne pathogens; thus, the environmental microbial community might impact pathogen survival via mixed biofilm formation. We collected floor drain samples from two beef plants with different E. coli O157:H7 prevalence history and investigated the effects of the environmental microorganisms on pathogen sanitizer tolerance. The results showed that biofilm forming ability and bacterial species composition varied considerably based on the plants and drain locations. E. coli O157:H7 cells obtained significantly higher sanitizer tolerance in mixed biofilms by samples from the plant with recurrent E. coli O157:H7 prevalence than those mixed with samples from the other plant. The mixed biofilm that best protected E. coli O157:H7 also had the highest species diversity. The percentages of the species were altered significantly after sanitization, suggesting that the community composition affects the role and tolerance level of each individual species. Therefore, the unique environmental microbial community, their ability to form biofilms on contact surfaces and the interspecies interactions all play roles in E. coli O157:H7 persistence by either enhancing or reducing pathogen survival within the biofilm community.

scholarly journals Results of production tests of the “Hyponat-BPO”

2021 ◽  
Vol 37 ◽  
pp. 00150
Author(s):  
Peter A. Popov ◽  
Irina V. Timofeeva ◽  
Svetlana A. Lavina
Keyword(s):  
Meat Processing ◽  
Auxiliary Equipment ◽  
Surface Disinfection ◽  
E Coli ◽  
Chemical Agents ◽  
Test Culture ◽  
Processing Plants ◽  
The Creation ◽  
Positive Effect ◽  
Effect Of Surface

The development of new detergent agents is mainly due to the creation of new compositions of chemical agents, which increases their cost. The article presents the results of production tests of the previously developed disinfection modes for refrigerators “Giponat BPO”. As a result of the production tests, it was found that the disinfectant “Hyponat BPO” provides 100% disinfection of refrigerated chambers, as well as auxiliary equipment at meat processing plants. Thus, it was found that the positive effect of surface disinfection when controlled by a test culture of E. coli was achieved by the use of a 2% solution with an exposure of 30 minutes, staphylococcus with a -3% solution and an exposure of 50 minutes and when controlled by a TMC -4% solution and an exposure of 60 minute.

Sensory, Deterioration and Bacteriological Assessment of Some Ready to Eat Poultry Products

2020 ◽  
Keyword(s):  
Meat Products ◽  
Thiobarbituric Acid ◽  
Poultry Meat ◽  
Meat Processing ◽  
Sensory Panel ◽  
E Coli ◽  
Poultry Products ◽  
Processing Plants ◽  
Hygienic Measures

This study was carried out to assess the quality of some RTE (ready to eat) chicken meat products collected from different poultry meat processing plants in Menofia and Gharbia governorate. a A total of 60 commercially produced RTE poultry products such as chicken ham, chicken shawarma, chicken with herps, smoked turkey and turkey smoked lobes (12 each) were collected from supermarkets and examined for sensory, deterioration criteria and bacteriological quality. The results revealed that the sensory panel scores of all examined RTE poultry meat products samples were generally low especially in chicken with herps, chicken ham and smoked turkey RTE poultry meat products. Moreover, pH values of most of the examined samples were relatively alkaline whereas the lowest value was recorded in smoked turkey samples and highest value was observed in chicken with herps samples. In addition, higher thiobarbituric acid (smoked turkey) and slightly increases of total volatile basic nitrogen values (chicken with herps) were recorded in most of the examined samples. All investigated bacterial counts were significantly high in all examined RTE poultry meat products samples. Staph. aureus was isolated from chicken ham and E. coli strains were isolated from chicken shawarma, smoked turkey and turkey smoked lobes. However, E. coli O157:H7 and salmonella strains failed to be isolated from all the examined samples. It could be concluded that all the examined RTE poultry meat products were deteriorated in terms of sensory, chemical and bacteriological terms and exceeded the limit described by E.S. (3493/2005) for cooked poultry meat products. In addition, these samples were probably produced and prepared under bad hygienic measures which could be responsible for lowering their quality and reduce shelf life.

ANALYSIS OF THE EFFECT OF PHAGES ON BACTERIA AND THEIR VIABLE BUT NONCULTURABLE CELLS

2021 ◽  
Vol 1 (3) ◽  
pp. 317-322
Author(s):  
T.A. Karachina ◽  
◽  
A.M. Abdullaeva ◽  
L.P. Blinkova ◽  
Yu.D. Pakhomov ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Raw Materials ◽  
Animal Origin ◽  
Meat Processing ◽  
Viable But Nonculturable ◽  
Chemical Substances ◽  
E Coli ◽  
Processing Plants ◽  
Nonculturable Cells ◽  
Alternative Means

The growth in the use of antibiotic and chemical substances in meat processing plants shows the need to search for alternative means of decontamination, the most promising of which are bacteriophages. However, there are a lot of little-studied questions on their use, one of which is the effect of bacteriophages on viable but nonculturable (VBNC) cells of bacteria. In this regard, the article provides data on the analysis of the activity of commercial bacteriophages in relation to bacteriacontaminants of raw materials of animal origin, in particular E. coli and Salmonella enterica typhimurium, as well as their VBNC-cells.

APPLICATION OF THE DISINFECTANT «ANOLYTE ANK-SUPER» FOR DISINFECTION OF SLAUGHTERHOUSES AND PRIMARY PROCESSING OF CATTLE

2018 ◽  
Vol 1 (1) ◽  
pp. 38-43
Author(s):  
M. P. Butko ◽  
◽  
P. A. Popov ◽  
S. V. Lemyaseva ◽  
D. A. Onischenko ◽  
...  
Keyword(s):  
Consumption Rate ◽  
Drug Consumption ◽  
Meat Processing ◽  
Laboratory Studies ◽  
Primary Processing ◽  
E Coli ◽  
Test Culture ◽  
Processing Plants ◽  
Positive Effect ◽  
Test Cultures

The article presents the results of laboratory studies on the development of disinfection regimes of the slaughterhouses and primary processing of cattle and the testing results of their efficiency in production conditions. The results of the research and production tests established that Anolyte ANK-super provides 100% disinfection of premises of slaughter and primary processing of cattle at meat processing plants and slaughterhouses. So determined, that the positive effect of disinfection of surfaces under control of the test cultures S. aureus and E. coli was achieved at the drug consumption rate 0,3…0,5 l/m2 and exposition of 90 minutes and under control of the test culture of B. cereus at the drug consumption rate of 0,5 l/m2, but exposition was 120 minutes. Materials of the conducted research allowed us to develop the «Technology of the disinfectant Anolyte ANK-super for veterinary-sanitary treatment of premises of slaughter and primary processing of cattle at meat processing plants and slaughterhouses».

Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities

1997 ◽  
Vol 60 (9) ◽  
pp. 1135-1138 ◽  
Author(s):  
SCOTT K. HOOD ◽  
EDMUND A. ZOTTOLA
Keyword(s):  
Stainless Steel ◽  
Cast Iron ◽  
Biofilm Formation ◽  
Epifluorescence Microscopy ◽  
Meat Processing ◽  
Isolation And Identification ◽  
Gram Negative ◽  
Plant Environment ◽  
Food Contact Surfaces ◽  
Processing Plants

Biofilms are described as a matrix of microorganisms which have adhered to and colonized a surface. Once formed, biofilms are difficult to remove and may be a source of contamination in food-processing environments. In this study, stainless-steel chips were fixed to surfaces adjacent to food-contact surfaces and cast-iron chips were suspended in the floor drains of four meat-processing plants. Biofilm formation was quantified by staining the attached cells and viewing them under epifluorescence microscopy. The stainless-steel and cast-iron chips removed from the plant environment showed some attached microorganisms. Floor drains appeared to provide an excellent environment for the formation of biofilms. Pseudomonas, Klebsiella, Aeromonas, and Hafnia species were identified as gram-negative microorganisms associated with the test surfaces.

scholarly journals Evaluating transmission dynamics of Shiga toxin-producing E. coli (STEC) in New Zealand cattle from farm to slaughter using PCR/MALDI-TOF and genome sequencing

2021 ◽  
Author(s):  
A. Springer Browne ◽  
Anne C. Midwinter ◽  
Helen Withers ◽  
Adrian L. Cookson ◽  
Patrick J. Biggs ◽  
...  
Keyword(s):  
New Zealand ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Shiga Toxin ◽  
Whole Genome ◽  
Meat Processing ◽  
Serious Illness ◽  
E Coli ◽  
Maldi Tof ◽  
Processing Plants

Cattle are asymptomatic carriers of Shiga toxin-producing E. coli (STEC) that can cause serious illness or death in humans. In New Zealand, contact with cattle feces and living near cattle populations are known risk factors for human STEC infection. Contamination of fresh meat with STECs also has the potential for rejection of consignments by importing countries. We used a combination of PCR/MALDI-TOF and whole genome sequencing to evaluate the presence and transmission of STEC on farms and in processing plants to better understand the potential pathways for human exposure and thus mitigate risk. Animal and environmental samples (n=2,580) were collected from six farms and three meat processing plants in New Zealand during multiple sampling sessions in spring of 2015 and 2016. PCR/MALDI-TOF analysis revealed 6.2% were positive for ‘Top 7’ STEC. ‘Top 7’ STEC were identified in all sample sources (n=17) tested. A marked increase in ‘Top 7’ STEC prevalence was observed between calf hides on-farm (6.3% prevalence), and calf hides at processing plants (25.1% prevalence). Whole genome sequencing was performed on ‘Top 7’ STEC bacterial isolates (n=40). Analysis of STEC O26 (n=25 isolates) revealed relatively low genetic diversity on individual farms, consistent with the presence of a resident strain disseminated within the farm environment. Public health efforts should focus on minimizing human contact with fecal material on farms and during handling, transport and slaughter of calves. Meat processing plants should focus on minimizing cross-contamination between the hides of calves in a cohort during transport, lairage and slaughter. Importance Cattle are asymptomatic carriers of Shiga toxin-producing E. coli (STEC), which can cause serious illness or death in humans. Contact with cattle feces and living near cattle are known risk factors for human STEC infection. This study evaluated STEC carriage in young calves and the farm environment with an in-depth evaluation of six farms and three meat processing plants over two years. An advanced molecular detection method and whole genome sequencing were used to provide a detailed evaluation of the transmission of STEC both within and between farms. The study revealed widespread STEC contamination within the farm environment, but no evidence of recent spread between farms. Contamination of young dairy calf hides increased following transport and holding at meat processing plants. The elimination of STEC in farm environments may be very difficult given the multiple transmission routes; interventions should be targeted at decreasing fecal contamination of calf hides during transport, lairage, and processing.

scholarly journals Use of ozone for dіcrease of microbal load

2020 ◽  
pp. 50-55
Author(s):  
M. Savcheniuk ◽  
B. Yarchuk ◽  
L. Korniienko ◽  
T. Tsarenko ◽  
D. Okhrimenko ◽  
...  
Keyword(s):  
Corona Discharge ◽  
Petri Dish ◽  
Bactericidal Effect ◽  
Operating Costs ◽  
Process Equipment ◽  
Main Method ◽  
E Coli ◽  
Pulsed Mode ◽  
Impact On Survival ◽  
Test Cultures

Ozone enters into chemical reaction with many organic compounds. Ozone is a very strong oxidant. It oxidizes most of the elements to higher oxides. In the oxidation reaction by ozone activity second only to fluorine, its oxide and free radicals. It is formed from oxygen by absorbing heat in this case, and, conversely, when the expansion goes into oxygen, giving off heat. The main method of obtaining ozone for practical purposes is electrosynthesis. In industrial conditions for using ozone and corona discharge barrier. Ozonizers corona discharge can significantly increase the efficiency of ozone by reducing capital and operating costs for the process equipment and ozonation. Research shows that ozone air ozonator pulsed mode increases the resistance of animals to the action of microorganisms factor. In animals and poultry in industrial production often develop diseases caused by pathogens factor. These diseases mainly occurring symptom of lesions of the respiratory and digestive systems. In many countries today conducted research using ozone, and the results of these studies are published in the course of scientific-metric database. For normal growing animals, especially young animals, premises should be thoroughly disinfected. Disinfection is required for all technological facilities as livestock and poultry. Experimentally found that ozone has a bactericidal effect (for example, test cultures E. coli) on different surfaces (wood, Petri dish) considering its concentration and exposure. The results of influence of different concentrations of ozone in ozonair mixture is fed into the container of the Petri dish, and time impact on survival of microorganisms provides a fairly accurate prediction of the results of impact parameters ozonation. Key words: ozon, ozone therapy, Escherichia coli, disinfection.

Prevalence of antibiotic resistant pathogenic E. coli from animals, retail and humans diagnosed with Gastroenteritisv

2021 ◽  
Author(s):  
Adriana M. Morales Gomez ◽  
Elizabeth Aguilera Nunez ◽  
Patrick McDonough ◽  
Yung-Fu Chang ◽  
Hussni O. Mohammed
Keyword(s):  
Supply Chain ◽  
Foodborne Pathogens ◽  
Food Supply ◽  
Phenotypic Expression ◽  
Meat Processing ◽  
Food Supply Chain ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Processing Plants ◽  
Genetic Detection

Foodborne pathogens represent one of the major challenges to health systems around the world. This risk is exacerbated by the presence of antimicrobial resistant (AMR) pathogens. Knowledge of the presence of these pathogens in the food supply chain would help in establishing intervention strategies to mitigate their risk. The objective of this study was to detect AMR among serotypes of Escherichia coli (E. coli) food adulterants serotypes of E. coli in the food supply chain and among isolates from gastroenteritis cases. E. coli isolates recovered from animals, meat processing plants, retail, and humans were examined for the presence of AMR using phenotypic and genotypic approaches. AMR to aminoglycosides, β-lactams, and tetracycline were detected in all isolates recovered from these sources at different levels. Similarly, presence of the bla-Tem, bla-SHV, aadA, and strAB genes were detected in isolates from these sources but there was no significant correlation between the genetic detection and phenotypic expression AMR.

scholarly journals Prevalence of verocytotoxigenicEscherichia coliin ground beef, pork, and chicken in southwestern Ontario

1990 ◽  
Vol 105 (1) ◽  
pp. 11-20 ◽  
Author(s):  
S. C. Read ◽  
C. L. Gyles ◽  
R. C. Clarke ◽  
H. Lior ◽  
S. McEwen
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Ground Beef ◽  
Meat Processing ◽  
E Coli ◽  
Serotype O ◽  
Retail Meats ◽  
Processing Plants ◽  
O 157 ◽  
Pork Samples

SUMMARYSamples of ground beef (225), pork (235) and chicken (200) were randomly selected from meat processing plants in the southwestern Ontario area. Supernatants of broth cultures of the samples were tested for verocytotoxins using a Vero cell assay. Neutralization of cytotoxic activity using antisera specific for three types of verocytotoxin (Verotoxin 1, Verotoxin 2 and Shiga-like toxin II) was performed on positive samples. Isolation of verocytotoxigenicEscherichia coli(VTEC) was attempted from positive samples. VTEC were confirmed asE. colibiochemically, tested for drug resistance, and serotyped. Based on neutralization studies, the prevalance of VTEC in beef and pork was at least 36·4% and 10·6%. respectively. This is much higher than has been reported from a survey of retail meats in which a method designed to detect onlyE. coliO 157.H7 was used. Isolations of VTEC were made from 10·4 % of the beef samples and 3·8 % of the pork samples. No VTEC were recovered from the chicken samples. The majority of VTEC isolates were susceptible to commonly used antimicrobial agents. A number of the serotypes of the VTEC isolates recovered have been associated with human disease; however, no VTEC of serotype O 157.H7 were isolated.

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