scholarly journals Білковий склад сироватки крові однорічок білого амура, уражених моногенеями, до та після застосування «Бровармектин-гранулятуTM» і «АвестимуTM»

2017 ◽  
Vol 19 (73) ◽  
pp. 131-135
Author(s):  
O.V. Fedorovych
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
High Efficiency ◽  
Thermal Power ◽  
Protein Composition ◽  
Fish Farm ◽  
The Body ◽  
Total Protein Content ◽  
Fish Farms ◽  
Antiparasitic Drugs

The success of treatment fish with invasive diseases depends on availability of highly efficient antiparasitic drugs in the pharmaceutical market. Therefore, scientists are constantly working to develop anti-parasitic drugs for the treatment of fish that would have not only high efficiency, less toxic to the body and the cost of medical treatment, but also contribute to the normalization of metabolic processes. That is why the purpose of our researches was to study the effect of «Granulated brovermectin» and «Avesstim» on the protein composition of blood serum of carp fish infested by various ectoparasites.Researches were conducted in garden-fish farms, SOE «Fish farm Halytskyy» (now LLC «Fish farm «Burshtynskyy») Rohatyn raion, Ivano-Frankivsk oblast and FE «Dobrotvir fish factory» Kamianka-Buzka Raion, Lviv oblast, located on the warm waters of cooling ponds of Burshtynsk and Dobrotvir Thermal Power Stations. Fish weighing 45–47 g were selected for researches.It was established that the use of «Granulated brovermectin» and immunomodulator «Avesstim» caused activation of protein metabolism in fish body affected by pathogens Dactylogyrus lamellatus, Gyrodactylus ctenopharyngodonis, and mixed infestations and as a result there was indicated the increase of total protein, albumins, globulins and α-globulins in their blood serum. Thus, the drug «Granulated brovermectin» in the blood serum of affected by ectoparasites fish total protein content compared with the control, increased to 4.23 (P < 0.001), albumins – to 2.82 (P < 0.001), globulins – to 1.41 (P < 0.05) and α-globulins – on 1.23 g/l (P < 0.01). Combined use of these drugs showed the best normalizing effect on the infested fish: the content of total protein in blood serum, depending on the type of infestation, increased to 5.22–9.97, albumins – to 4.34–8.17, globulins – 0.88–1.45, α-globulins tо – 1.36–2.20 g/l, and these changes in all cases (the exception – the quantity of globulins in the blood of fish affected by gyrodactylus) were reliable (P < 0.05–0.001).

scholarly journals Changes in the composition of proteins in the blood during the period of ischemia-reperfusion syndrome, modeled by a violation of blood flow in the liver

2018 ◽  
Vol 20 (2) ◽  
pp. 110-114
Author(s):  
G Sh Garayev ◽  
J R Gafarova ◽  
R E Jafarova
Keyword(s):  
Total Protein ◽  
Plasma Proteins ◽  
Ischemia Reperfusion ◽  
Protein Composition ◽  
The Body ◽  
Total Protein Content ◽  
Quantitative Composition ◽  
Liver Ischemia ◽  
Pathological Changes ◽  
Quantitative Changes

On the experimental model of liver ischemia-reperfusion, quantitative changes in protein composition in the blood of white outbred rats were investigated. It was found that against the background of a 10-minute ischemia, the total protein content decreased by 0,9%, albumins by 1,5% (p0,05). The content of globulins practically did not change, and the content of fibrinogen increased by 33,3% (p0,05). In the corresponding periods of reperfusion compared with intact values, the content of total protein and albumins decreased, and globulins and fibrinogen increased. The intensity of changes in the quantitative and qualitative content of proteins in the blood of animals depended on both the duration of the ischemia period and the duration of reperfusion. At the same time, the increase in the level of globulins in the blood occurred more intensively during reperfusion after 20 minutes of ischemia. Thus, against the background of ischemia, there are changes in the quantitative composition of plasma proteins (total protein, albumins, globulins, fibrinogen). As the duration of ischemia increases, pathological changes increase. With reperfusion, the process continues unidirectionally, but against the background of a deeper ischemia lasting 20 minutes, some parameters of the pathological changes in the blood composition are less pronounced than against a background of reperfusion after a 10-minute ischemia. This phenomenon is explained by the activation of the protective and adaptive functions of the body.

scholarly journals Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

2020 ◽  
Vol 3 (2) ◽  
pp. 42-46
Author(s):  
V. G. Stoyanovskyy ◽  
M. O. Shevchuk ◽  
I. A. Kolomiiets ◽  
V. A. Kolotnytskyy
Keyword(s):  
Total Protein ◽  
Protein Metabolism ◽  
Broiler Chickens ◽  
Stress Factor ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Combined Stress ◽  
Stages Of Development ◽  
Γ Globulins

The body of  broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.

scholarly journals FURTHER STUDIES ON THE CHEMISTRY OF BLOOD SERUM

1913 ◽  
Vol 17 (4) ◽  
pp. 444-452 ◽  
Author(s):  
Albert A. Epstein
Keyword(s):  
Blood Serum ◽  
Protein Content ◽  
Protein Composition ◽  
The Other ◽  
Total Protein Content ◽  
Protein Nitrogen ◽  
Standard Values ◽  
The Individual ◽  
Chronic Interstitial Nephritis ◽  
Functional Deficiency

The following conclusions may be drawn from the results obtained in this study. 1. In the minor surgical cases (considered normal) the chemical composition of the blood serum agrees, as far as its proteins are concerned, with the usual standard values. When examined at different times, the serum of such cases shows no variation in the total protein content or in its individual fractions. The incoagulable nitrogen, however, varies considerably in the total amount in the different cases, as well as in its percentage relations to the other constituents of the serum. 2. The cases of prostatic hypertrophy, with or without chronic interstitial nephritis, show no change from the normal in the character of the protein composition of the serum, nor in the ratio which the individual fractions bear to each other. On the other hand, the incoagulable and non-protein nitrogen of the serum show marked fluctuations, some of which correspond to the degree of functional deficiency of the kidneys. 3. In cases of localized infections of the kidneys, the changes in the blood serum are twofold. One concerns the proteins, and is traceable to the infection; and the other concerns the non-protein nitrogen, results from functional impairment, and varies with the amount of destruction of the kidney substance. Thus an increase in the globulins is observed in these sera similar to that occurring in infections in other localities; the non-protein content increases apparently in direct proportion to the degree of deficiency of the kidney, and becomes diminished when the function of the kidneys improves.

scholarly journals Investigation of Protein and Epitope Characteristics of Oats and Its Implications for Celiac Disease

2021 ◽  
Vol 8 ◽  
Author(s):  
Gyöngyvér Gell ◽  
Zsuzsanna Bugyi ◽  
Christakis George Florides ◽  
Zsófia Birinyi ◽  
Dalma Réder ◽  
...  
Keyword(s):  
Total Protein ◽  
Estimation Method ◽  
Protein Composition ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Total Protein Content ◽  
Sample Population ◽  
Gluten Free ◽  
Spectrometric Data ◽  
Rp Hplc

The use of pure oats (oats cultivated with special care to avoid gluten contamination from wheat, rye, and barley) in the gluten-free diet (GFD) represents important nutritional benefits for the celiac consumer. However, emerging evidence suggests that some oat cultivars may contain wheat gliadin analog polypeptides. Consequently, it is necessary to screen oats in terms of protein and epitope composition to be able to select safe varieties for gluten-free applications. The overall aim of our study is to investigate the variability of oat protein composition directly related to health-related and techno-functional properties. Elements of an oat sample population representing 162 cultivated varieties from 20 countries and the protein composition of resulting samples have been characterized. Size distribution of the total protein extracts has been analyzed by size exclusion-high performance liquid chromatography (SE-HPLC) while the 70% ethanol-extracted proteins were analyzed by RP-HPLC. Protein extracts separated into three main groups of fractions on the SE-HPLC column: polymeric proteins, avenins (both containing three subgroups based on their size), and soluble proteins, representing respectively 68.79–86.60, 8.86–27.72, and 2.89–11.85% of the total protein content. The ratio of polymeric to monomeric proteins varied between 1.37 and 3.73. Seventy-six reversed phase-HPLC-separated peaks have been differentiated from the ethanol extractable proteins of the entire population. Their distribution among the cultivars varied significantly, 6–23 peaks per cultivar. The number of appearances of peaks also showed large variation: one peak has been found in 107 samples, while 15 peaks have been identified, which appeared in less than five cultivars. An estimation method for ranking the avenin-epitope content of the samples has been developed by using MS spectrometric data of collected RP-HPLC peaks and bioinformatics methods. Using ELISA methodology with the R5 antibody, a high number of the investigated samples were found to be contaminated with wheat, barley, or rye.

scholarly journals Marker changes of blood plasma proteinogram in rats with toxic hepatitis

2020 ◽  
Vol 11 (3) ◽  
pp. 360-366
Author(s):  
V. A. Gryshchenko ◽  
V. S. Minina
Keyword(s):  
Blood Plasma ◽  
Plasma Protein ◽  
Total Protein ◽  
Toxic Hepatitis ◽  
Diclofenac Sodium ◽  
The Body ◽  
Laboratory Animals ◽  
Total Protein Content ◽  
Protein Spectrum ◽  
G Ratio

In recent years, there has been a pronounced tendency to increase in the incidence of drug-induced liver damage due to the growing expansion of the pharmaceutical market, which is also observed in the case of incorrect administration of nonsteroidal anti-inflammatory drugs (NSAIDs). In this case, the violation of the functional state of the body has a negative effect on synthetic processes, which in combination with the protein system of tissues significantly affects the metabolic homeostasis of the body. Therefore, the aim of the study was to determine marker changes in the plasma protein spectrum in laboratory rats with diclofenac-induced hepatitis and the effectiveness of reparative therapy based on milk phospholipids. The drug form of toxic hepatitis in laboratory animals was induced according to the author’s model by oral administration of diclofenac sodium (NSAID group) at a dose of 12.5 mg/kg, once a day for 14 days. Thus, in rats with toxic hepatitis there was a probable decrease in plasma total protein content by 15.6% compared with control, indicating a violation of protein-synthesizing function of the liver. With the introduction into the body of clinically healthy and sick animals of the liposomal form of the bioadditive "FLP-MD" based on milk phospholipids, the level of total protein in blood plasma corresponded to control values. As a result of the study of the plasma protein spectrum of Wistar rats, the four most sensitive indicators, which undergo significant probable changes in absolute and relative units of measurement with the development of toxic diclofenac-induced hepatitis, are protein fractions with molecular weights of 180–190, 150–170, 60 and 54–58 kDa and four markers of the effectiveness of restoring the protein-synthesizing function of the liver with the use of corrective therapy, in particular, bioadditives "FLP-MD" – 900, 180–190, 68–70 kDa and the value of A/G ratio, which is important for implementation in applied veterinary medicine, especially in the diagnosis of NSAID hepatopathy, supplementing the picture of its pathogenesis at the molecular level and testing the effectiveness of newly created drugs of hepatoprotective profile.

scholarly journals Dynamics of total protein content in blood of sows depending on peculiarities of nervous system activity

2019 ◽  
Vol 21 (94) ◽  
pp. 51-56
Author(s):  
R. Postoi ◽  
V. Karpovskyi ◽  
O. Danchuk ◽  
D. Kryvoruchko
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Blood Serum ◽  
Protein Content ◽  
Total Protein ◽  
Weak Type ◽  
Nervous Activity ◽  
Total Protein Content ◽  
Experimental Animals ◽  
Higher Nervous Activity

The article presents the results of studies on the dynamics of total protein content in blood serum of sows depending on the cortical and vegetative mechanisms of regulation under exposure to technological stimulus. Experiments were carried out on 3 years old pigs of large white breed. Types of higher nervous activity in pigs were determined using method of conditioned food reflexes. According to the studying of conditioned reflex activity 4 experimental groups were formed, 5 animals in each. The 1-st group consisted of sows with strong balanced mobile, the 2-nd – strong balanced inert, the 3-rd – strong unbalanced, and the 4-th – weak types of higher nervous activity. Then in experimental animals we studied the tone of the autonomic nervous system using trygeminovagal test, by the results of which we formed 3 experimental groups (normotonics, sympathicotonics, vagotonics), 5 animals in each. Before exposure to technological stimulus and in 1, 3, 7, 14 and 28 days after its impact, blood samples were taken for biochemical studies in all experimental animals. Before exposure to technological stimulus content of total protein in swine blood serum between animals with strong types of higher nervous activity did not differ significantly. Animals with weak type of higher nervous activity had lower level of this metabolite than animals with strong balanced mobile type. After exposure to technological stimulus the total protein level in swine blood serum significantly decreased. In sows with strong balanced mobile type, the content of total protein starting from the 7-th day after exposure to technological stimulus returned to values that were before stress factor impact. At the same time, in sows with weak type of higher nervous activity, the level of total protein in blood returned to the reference values only in 28 days after exposure to technological stimulus. The basal level of total protein in blood serum in pigs with different tone of autonomic nervous system didn’t differ significantly. In sows normotonics was found a significantly higher content of total protein in blood serum than in sows vagotonics and sympathicotonics during first week after exposure to stress factor.

scholarly journals Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood

2021 ◽  
Vol 23 (101) ◽  
pp. 21-25
Author(s):  
L. V. Koreyba
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Mineral Metabolism ◽  
Obstetric Care ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The State ◽  
Total Protein Content ◽  
Carotene Content

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.

scholarly journals State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates

2019 ◽  
pp. 85-94
Author(s):  
V. Sakara ◽  
A. Melnyk ◽  
F. Markhenkov
Keyword(s):  
Total Protein ◽  
Broiler Chickens ◽  
Zinc Content ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Experimental Group

The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.

scholarly journals The effect of convulsive syndrome on the metabolic status and survival of patients with alcoholic delirium

2020 ◽  
pp. 47-50
Author(s):  
Volodymyr Zadorozhnyi
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Delirium Tremens ◽  
Young Patients ◽  
The Body ◽  
Metabolic Status ◽  
Total Protein Content ◽  
Daily Excretion ◽  
Somatic Disorders ◽  
Severity Of The Disease

The aim of the study was to study the effect of convulsive syndrome in patients with delirium tremens (DT) on the charac teristics of the metabolic status of the body, the severity of clinical manifes tations, and survival of people of different ages. The study involved 753 patients with DT at the age of 20 to 76 years. The metabolic status was studied. One indicator was the urea-creatinine serum index; its low level was considered a sign of anabolic trends in metabolism. Another indicator was the determination of the total protein content in the body by daily excretion of creatinine. It was found that patients with DT with convulsive syndrome had a higher score according to the severity of the disease according to the DRS scale, a longer period of mental disorders, the average number of somatic complications was higher. However, the survival rate of patients with DT with convulsive syndrome and patients with DT without convulsive manifestations was the same. To determine the causes of this, the metabolic status and age of surviving and deceased patients with DT were compared. The highest total protein content in the body is observed in surviving patients with DT with manifestations of convulsive syndrome. Surviving patients with DT with manifestations of convulsive syndrome had a significantly lower level of urea-creatinine index compared with survivors with DT without convulsive syndrome. Among all patients with DT, the average age of survivors with convulsive syndrome was the lowest, and the average age of deceased patients with convulsive syndrome was the highest. In patients with DT without convulsive manifestations, the age of surviving and dead patients did not differ. The age of surviving patients with DT with convulsive syndrome is significantly lower than that of surviving patients without convulsive manifestations. The presence of convulsive syndrome and associated metabolic changes in the body in patients with DT contributes to the survival of a younger part of patients. Key words: alcohol delirium, seizures, aggravation of mental and somatic disorders, anabolic state of metabolism, primary survival of young patients

scholarly journals The effect of cysticercosis invasion on the protein metabolism and cellular immunity of rabbits

2021 ◽  
Vol 23 (1) ◽  
pp. 7-11
Author(s):  
Y. V. Duda ◽  
Keyword(s):  
Cellular Immunity ◽  
Total Protein ◽  
Protein Metabolism ◽  
The Body ◽  
Total Protein Content ◽  
Blood Proteins ◽  
Visual Identification ◽  
Γ Globulins ◽  
Inflammatory Processes ◽  
Healthy Control

The composition of blood proteins depends on the functional state of animals, as well as on various pathologies. The goal of the work was to establish the effect of cysticercosis invasion on the protein metabolism and cellular immunity of rabbits. Rabbits were divided into two groups after visual identification of the larval cysts presence: healthy (control) and diseased (experimental). Biochemical blood tests were performed using reagent sets produced by “Filisit-Diagnostika” (Ukraine). Spontaneous cysticercosis invasion of male rabbits caused changes in protein metabolism. The content of total protein in infected animals was higher by 8.79% (P<0.05) than in control. The increase in the total protein content was due to the globulin fraction, which was 1.50 times (P<0.05) higher in affected animals than in healthy rabbits. This rise occurred as a result of a high content of γ-globulins, both in absolute value by 1.69 times (P<0.05), and in percentage by 1.51 times (P<0.05). The albumins, which are completely synthesized by liver, were lower by 10.08% (P<0.05) in rabbits with cysticercosis. The protein coefficient of experimental animals was lower by 43.89% (P<0.05) compared with the control. The concentration of uric acid in diseased rabbits was lower by 34.09% (P<0.05) than in healthy ones. We have observed a high number of T-, B-lymphocytes and T-helper cells, especially the last two indicators, which increased respectively 1.54 times (P<0.01) and 1.36 times (P<0.05) against the background of a low number of T-suppressors and O-lymphocytes — almost 5 times (P<0.001) in affected animals than in healthy rabbits. In our opinion, such changes in the proteinogram and cellular immunity indicate the intensification of immune reactions in the body of rabbits infected with the Cysticercus pisiformis as a result of inflammatory processes that occur in the liver under the action of a mechanical, toxic effect of the helminths.

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