scholarly journals State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates

2019 ◽  
pp. 85-94
Author(s):  
V. Sakara ◽  
A. Melnyk ◽  
F. Markhenkov
Keyword(s):  
Total Protein ◽  
Broiler Chickens ◽  
Zinc Content ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Experimental Group

The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.

scholarly journals BIOLOGICAL EFFECTS OF SILICON OXIDE NANOPARTICLES ON BROILER CHICKEN

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Silicon Oxide ◽  
The Body ◽  
Control Group ◽  
The Third ◽  
Experimental Group

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.

scholarly journals Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

2020 ◽  
Vol 3 (2) ◽  
pp. 42-46
Author(s):  
V. G. Stoyanovskyy ◽  
M. O. Shevchuk ◽  
I. A. Kolomiiets ◽  
V. A. Kolotnytskyy
Keyword(s):  
Total Protein ◽  
Protein Metabolism ◽  
Broiler Chickens ◽  
Stress Factor ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Combined Stress ◽  
Stages Of Development ◽  
Γ Globulins

The body of  broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.

scholarly journals The influence of Zinc and Manganese chelates (pantothenates) on some chicken broiler metabolism indicators

2019 ◽  
Vol 21 (96) ◽  
pp. 134-140
Author(s):  
V. S. Sakara ◽  
A. Yu. Melnyk
Keyword(s):  
Weight Gain ◽  
Amino Acid ◽  
Total Protein ◽  
Broiler Chickens ◽  
Biochemical Study ◽  
Zinc Content ◽  
Manganese Concentration ◽  
Control Group ◽  
University Training ◽  
Experimental Group

The article presents the results of the use of the vitamin-amino acid chelates (pantothenates) of Zinc and Manganese in order to study their effect on some indicators of avian metabolism. The studies were started on 14-day Cobb 500 broiler chickens in the poultry farm of the Bila Tserkva National Agrarian University Training and Production Center. For this purpose, 3 groups poultry were formed: two experimental ones (Zinc and Manganese chelates were fed to the main diet with water) and a control group (50 heads each). During the experiment (14, 21, 28 days), weighed chickens followed by blood sampling for biochemical study. In the group where the chelates were drunk at a dose of 0.2 ml/l of water over the next 14 days, there was a tendency to increase the absolute weight gain in broiler chickens to 943.0 ± 25.94 g (770–1073), compared with the control – 883.2 ± 24.64 g (740–1140). The bird, which was given chelates at a dose of 0,1 ml/l of water for 14 days, increased the content of total protein in the serum by 10.3% compared to the beginning of the study, and was 30.9 ± 0.71 g/l (P < 0.001). In the second group (chelate dose of 0.2 ml/l water), this indicator increased by 11.2% and amounted to 30.2 ± 0.82 g/l (P < 0.01). At the end of the experiment (28 days of cultivation), the albumin content in the blood of the birds of the first and second experimental groups was 18.6 ± 0.36 and 18.7 ± 0.37 g/l (P < 0.001 before the first selection). Manganese concentration in broiler chickens of the second experimental group on the 28th day was 3.6 ± 0.28 μmol/l and was higher than the control (2.7 ± 0.25 μmol/l; P < 0.01). At doses of 0,1 ml/l of water (1st experimental group), there was a tendency to increase the amount of this trace element in serum to 3.2 ± 0.16 μmol/l (1.4–4,0). After 14 days of chelating, Zinc content in the serum of chickens of the first experimental group increased by 20% and amounted to 27.9 ± 0.60 μmol/l, in its second – concentration increased by 25.8% (28.3 ± 0.76 μmol/l; P < 0.001) compared to the start of the experiment. Therefore, the use of the vitamin-amino acid chelates of Zinc and Manganese for 14 days at a dose of 0,1 and 0.2 ml/l of water contributed to the increase in weight gain, increase of total protein, Manganese and Zinc in the serum of broiler chickens.

scholarly journals Біохімічні показники крові свиноматок за профілактики порушень обміну мінеральних речовин

2017 ◽  
Vol 19 (82) ◽  
pp. 71-76 ◽  
Author(s):  
N.G. Grushanska ◽  
V.М. Kostenko
Keyword(s):  
Blood Serum ◽  
Protein Content ◽  
Total Protein ◽  
Calcium Concentration ◽  
Emission Spectrometry ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Pig Production ◽  
Experimental Group

Of all domestic animals, pigs suffer the most from deficiency of minerals. This is due to the specificity of pig production, feeding technology, retention and anatomical – physiological characteristics of pigs. In order to obtain the best qualities of pig production, it is necessary to make correction the ration for swines of various technological groups using mineral feed additives that take into consideration not only the physiological needs for minerals of the corresponding age groups and classes of swines but also the biogeochemical zone or the province where the farm is located. Also, conditions that accompany the emergence of a mineral deficit are taken into account. The development of new, eco-friendly, non-toxic means of preventing disorders of metabolism of minerals in pigs is a promising direction of veterinary medicine.The research was carried out ont the farm of the Kyiv region (northeastern biogeochemical zone). Biochemical parameters of blood were studied using biochemical analyzer «Labline-010» with standard sets of reagents. The content of chemical elements in water was investigated by the method of atomic emission spectrometry on the Optima 210 DV device.The paper presents the authors’ own research on determining the biochemical status of an organism of lactating sows in the northeastern biogeochemical zone of Ukraine at the prevention of microelementosis using new experimental eco-friendly means.The content of total protein, albumin, glucose, total calcium, inorganic phosphorus, cholesterol, bilirubin, activity of ALT, AST, AF in the blood serum of sows of the northern-eastern biogeochemical zone of Ukraine before and after the application of experimental drugs was investigated. The positive influence of prophylactic agents on the parameters of metabolism of proteins and calcium was defined.In the blood serum of the sows of the first experimental group, 28 days later, the total protein content on 5.3% lower and the total calcium concentration on 16.5% higher than the control group  of animals were found.In the blood serum of sows in the second experimental group,  28 days later, the total protein content on 8.3%  lower and the total calcium concentration on 19.9% higher than the control group of animals were found.

The effect of ethanol on some hematological parameters of the australian red claw crayfish (Cherax quadricarinatus)

2022 ◽  
pp. 85-91
Author(s):  
D. Skafar ◽  
D. Shumeyko
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Hematological Parameters ◽  
Group Versus ◽  
Control Group ◽  
Total Protein Content ◽  
Cherax Quadricarinatus ◽  
Total Hemocyte Count ◽  
Red Claw Crayfish ◽  
Experimental Group

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.

scholarly journals The effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats under prolonged cadmium and lead loading

2020 ◽  
Vol 22 (100) ◽  
pp. 54-59
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
P. S. Hnativ ◽  
S. D. Murska
Keyword(s):  
Liver Function ◽  
Functional State ◽  
Total Protein ◽  
Sodium Selenite ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Physiological Values ◽  
Cadmium And Lead ◽  
Experimental Group

In the conditions of modern technogenic pollution of the environment, environmental problems, improving the quality of livestock products and their food safety is an important and urgent issue today. Pollution of the environment with heavy metals due to man-made activities of the population has led to a number of problems of agricultural production. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats in lead-cadmium intoxication. It was found that the level of total protein in the blood of rats exposed to cadmium and lead was reduced. These changes were due to a decrease in albumin levels and an increase in globulin levels. Thus, hypoproteinemia resulting from cadmium and lead poisoning may indicate impaired hepatic protein synthesis. Transamination enzymes play a major role in the relationship between protein and carbohydrate metabolism, which is important for switching the oxidation of energy substrates from glucose to amino acid energy nutrition, which is extremely important under the influence of various environmental factors. The most active transaminases are aspartate aminotransferase and alanine aminotransferase. In the study of the activity of aminotransferases in the serum of rats treated with lead acetate and cadmium chloride, an increase in the activity of enzymes in all periods of the study. The highest activity of aminotransferases was on days 21 and 28 of the experiment in the control group of rats, which carried out the combined load of cadmium and lead. Thus, the load of the body of animals with cadmium and lead contributes to the violation of the functional state and protein-synthesizing function of the liver of animals of the experimental groups. With the introduction of sodium selenite into the body of rats, the activity of ALT and AST in the serum of the first experimental group was slightly reduced compared with the control group. However, the activity of these enzymes did not reach physiological values. When setting the feed additive “Metisevit plus” throughout the experiment, it was found that the activity of aminotransferases in the serum of rats of the second experimental group ranged within physiological values. This is due to the hepatoprotective effect of the components of the feed additive “Metisevit Plus”. In the study of protein-synthesizing function of rat liver in experimental lead-cadmium toxicosis, it was found that after application to rats of the first experimental group of sodium selenite on days 14, 21 and 28, the level of total protein in serum gradually increased, but did not reach physiological values. Using the feed supplement “Metisevit plus” rats of the second experimental group, found a slightly higher level of total protein in their blood, which on the 28th day of the experiment reached physiological values and, accordingly, amounted to 66.8 ± 1.99 g/l. It should be noted that when administering the feed additive “Metisevit plus” protein-synthesizing liver function and liver function of rats of the second experimental group were restored faster than when administering sodium selenite to the first experimental group.

scholarly journals Features of protein metabolism in the body of broiler chickens when using the feed additive Peak-Antistress in the diet

2020 ◽  
pp. 103-110
Author(s):  
A. V. Mitfakhutdinov ◽  
E. R. Saifulmuliukov
Keyword(s):  
Broiler Chickens ◽  
Protein Composition ◽  
Feed Additives ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Protein Accumulation ◽  
White Meat ◽  
Scientific Publications ◽  
Experimental Group

Scientific publications describe the positive effect of feed additives and pharmacological complexes on metabolic processes in the body of broiler chickens, in particular, on the protein composition of the blood, meat productivity and protein accumulation in meat. The Peak-Antistress feed additive developed at the Department of Morphology, Physiology and Pharmacology of the South Ural State Agrarian University allows to reduce the technological load on the poultry body by stimulating the general metabolism and complex antioxidant action. Experiments on the use of the feed additive Peak-Antistress were carried out on broiler chickens of the final hybrid of the Arbor Acres cross in an industrial-type poultry farm with floor technology. The poultry was divided into three groups of 6,000 chickens each and kept in one workshop in separate sections. The control group received the main diet, the 1st experimental group received the main diet and feed additive at a dose of 1269 g / t of feed 5 days before slaughter, the 2nd experimental group received the main diet and feed additive at a dose of 1693 g / t of feed 5 days before slaughter. Slaughter of broiler chickens was carried out according to the technological instructions of the enterprise on the 38th day. Against the background of the use of the feed additive in the experimental groups of broiler chickens, stimulation of protein metabolism was observed, as evidenced by an increase in total protein in the blood by 7.9–20.1%, an increase in the level of protein in white meat by 0.1–0.3%, and a tendency to an increase in amino acids in white meat protein by 0.2–12.3%.

scholarly journals Protein-synthesizing function of the liver of cows at experimental fasciolosis

2021 ◽  
Vol 4 (1) ◽  
pp. 12-15
Author(s):  
D. V. Frejuk ◽  
V. V. Stybel
Keyword(s):  
Functional State ◽  
Causative Agent ◽  
Total Protein ◽  
The Body ◽  
Control Group ◽  
Blood Proteins ◽  
Functional Connection ◽  
Animal Blood ◽  
The World ◽  
Experimental Group

Cattle fasciolosis is quite widespread in the world, including in Ukraine. Animal blood proteins, being in close functional connection with proteins of various tissues, reflect the changes that occur in the tissues and organs of the body during disorders of their metabolic processes caused by pathological factors. The aim of the study was to investigate the level of total protein and its fractions in the blood of cows in experimental fasciolosis. 12 cows of 4–5 years of age, black-spotted breed, were selected for experiment, out of which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed - selection and keeping of analogue animals in groups. The cows' diet was balanced in terms of nutrients and minerals. In the study of protein-sensitizing function of the liver of cows, it was found that during fasciolosis invasion, the content of total protein decreased by 13.8 %. These changes were due to a decrease in albumin and an increase in globulins. Thus, in fasciological invasion of cows, the level of albumin in the serum decreased by 24.4 %, and the level of globulins increased by 7.9 %. The value of the albumin / globulin ratio in the blood of infested cows with the causative agent of fasciolosis is quite indicative. It was found that the albumin-globulin ratio in the blood of cows of the experimental group was probably lower compared to the control group of animals. This value of the coefficient, of course, indicates the suppression of protein-synthesizing function of the liver in these periods of research. In the future it is planned to investigate the functional state of the liver of cows with experimental fasciolosis.

scholarly journals The biochemical indicators of a cow organizm at the prevention of microelementosis

2018 ◽  
Vol 20 (83) ◽  
pp. 183-188
Author(s):  
N.G. Grushanska
Keyword(s):  
Total Protein ◽  
Chemical Elements ◽  
Organic Phosphorus ◽  
Zinc Content ◽  
Emission Spectrometry ◽  
Control Group ◽  
Total Calcium ◽  
Creatinine Concentration ◽  
Animal Group ◽  
Mineral Substances

Correction of cattle feed, taking into account the physiological needs of their organism in the mineral substances of the corresponding biogeochemical zone or the province, where the farm is located, as well as the conditions that accompany the emergence of the deficit, promotes the obtaining of quality livestock products. An urgent task of the present is the search for eco-friendly, non-toxic and highly effective, preventive drugs of complex action, which positively affect the metabolism of mineral substances in the animal organism. The research was carried out on the farm of Kyiv region (central biogeochemical zone). We studied the biochemical parameters of blood and saliva using biochemical analyzer «Labline-010» with standard sets of reagents. The content of chemical elements in blood and saliva was investigated by the method of atomic emission spectrometry with inductively coupled plasma using Optima 210 DV device. The paper presents the research on determining the biochemical status of an organism of lactating cows at the prevention of microelementosis using new experimental eco-friendly drug. The content of total protein, albumin, glucose, total calcium, non-organic phosphorus, cholesterol, total bilirubin, aluminium, chrome, copper, zinc, nickel and activity of ALT, AST, ALP, GGT in cow blood and saliva in the first and 28th day for the use of the drug oxyminkor. The diagnostic informativity of saliva content according to the content of mineral substances is determined. In cow blood at the use of the drug oxyminkor for 28th day of the experiment, the content of total protein was determined to be 17% higher, albumin was 19% higher, total calcium was 36 % higher and creatinine concentration was 25% lover, compared with the indicators of the control group. In the cow saliva at the use of the drug oxyminkor on the 28th day of the experiment, the content of zinc was 2.1 times higher, compared with the control animal group. The correlation coefficient for zinc content in cow blood and saliva was 0.80, which points the informativity of this indicator. We defined the positive influence of the prophylactic agent on the metabolism of proteins, minerals and functional state of the cow liver. The introduction of new methods of non-invasive diagnosis and the development of ecological, non-toxic agents for the prevention of mineral disbolism among cows is a promising area of research.

scholarly journals Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood

2021 ◽  
Vol 23 (101) ◽  
pp. 21-25
Author(s):  
L. V. Koreyba
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Mineral Metabolism ◽  
Obstetric Care ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The State ◽  
Total Protein Content ◽  
Carotene Content

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.

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