scholarly journals Symbiotic effect on some microbiological species and physicochemical properties in milk in subclinical mastitis of dairy cows

2020 ◽  
Vol 12 (4) ◽  
pp. 364-370
Author(s):  
I. Abdennebi ◽  
C.R. Messai ◽  
N. Ouchene ◽  
N.A. Ouchene-khelifi ◽  
L. Ghallache ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Physicochemical Characteristics ◽  
Subclinical Mastitis ◽  
Coli Strain ◽  
Coagulase Negative Staphylococcus ◽  
Microbiological Analysis ◽  
Cattle Farm ◽  
California Mastitis Test ◽  
Development Of Resistance ◽  
Before And After

Abstract. Subclinical mastitis (SCM) is the most common form of mastitis and the greatest cause for concern in dairy cows. The overuse of antibiotics for the treatment of mastitis leads to the development of resistance, resulting in the use of symbiotics. The study was carried out between February and May 2017 at a pilot dairy cattle farm in the Tipaza region (north-central Algeria) aiming to investigate the effect of a symbiotic on SCM. California Mastitis test (CMT) was used to diagnose SCM in a total of 240 dairy cows. A number of 58/240 (24.16%) cows were found to have SCM. These mastitis cows were then divided into two lots; an experimental lot of 37 cows and a control lot of 21 cows. A symbiotic was administered to the experimental lot once a month for three months. Cell count, microbiological analysis and analysis of certain physicochemical parameters of the milk were applied before and after each administration of the symbiotic. The results revealed that the average somatic cells count (SCC) in cows from the control lot was higher than that of cows from the experimental lot throughout the study period (p<0.0001). Staphylococci were isolated from 51/58 (87.93%) of the mastitis cows, of which 21 (36.20%) were infected with Staphylococcus aureus and 30 (51.72%) with coagulase-negative Staphylococcus. Enterobacteriaceae were isolated from 36/58 (62.07%) of the mastitis cows, of which 21 (36.20%) were due to Escherichia coli strain and 15 (25.86%) to other strains of Enterobacteriaceae. After administration of the symbiotic, the prevalence of S. aureus and E. coli decreased significantly in the experimental lot compared to the control lot (p<0.001). The physicochemical characteristics of the milk were not altered by the administration of the symbiotic. All these results show that the symbiotic constitutes an adequate solution to replace antibiotics in the treatment of SCM.

scholarly journals Prevalence and Predisposing factors of Subclinical Mastitis in Dairy Cows in Bakir, Sukomulyo Village, Batu Regency

2019 ◽  
Vol 1 (2) ◽  
pp. 35-41
Author(s):  
Mira Fatmawati ◽  
◽  
Ani Setianingrum ◽  
Ajeng Erika Prihastuti Haskito ◽  
Fidi Nur Aini Eka Puji Dameanti ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Economic Loss ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cross Sectional Study ◽  
Predisposing Factors ◽  
Cross Sectional ◽  
California Mastitis Test ◽  
Source Of Infection ◽  
Before And After

Udder inflammation in dairy cows known as mastitis is still a major problem in dairy industry. Economic loss caused by mastitis burden on farmers due to decrease on milk yield and decrease on the milk quality. Most of traditional farmers recognize clinical mastitis as the symptoms was obvious, while subclinical mastitis were not easy to detect and can be potential source of infection for the herd. Detection on subclinical mastitis were using indirect test california mastitis test (CMT) and direct test with Somatic Cell Count (SCC). This research was a cross sectional study using udder quarters milk sample from traditional dairy farms in Bakir, Sukomulyo village, Batu regency. Field test conduct uding CMT and SCC counting in laboratorium. Data collection using Questionnaire were conduct to asses predisposing variables on farmers. Result shows prevalence of subclinical mastitis based on SCC was 60,22% and based on CMT 18,20% of quarter sampels. Predisposing factors that have effect on mastitis were the sanitation of the cow and the farm before and after the milking process.

scholarly journals Prevalence and Bacterial Isolates of Mastitis in Dairy Farms in Selected Districts of Eastern Harrarghe Zone, Eastern Ethiopia

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Tesfaheywet Zeryehun ◽  
Gerema Abera
Keyword(s):  
Dairy Cows ◽  
Bacterial Pathogens ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococcus ◽  
Eastern Ethiopia ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Associated Risk Factors ◽  
Standard Techniques

The study was conducted from November 2015 to April 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of Eastern Harrarghe Zone, Eastern Ethiopia. The study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, California mastitis test (CMT), and isolation bacterial pathogens using standard techniques. In the present study the overall mastitis at cow level was 247 (64.3%). The prevalence of clinical and subclinical mastitis and quarter level prevalence for clinical and subclinical mastitis were 12.5% and 51.8% at cow level and 10.7% and 46.4% at quarter level, respectively. Clinically, 101 (6.6%) quarters which belong to 75 (19.5%) animals were found to be with blind teat. In the present study prevalence of mastitis was significantly associated with parity and age (p<0.05). Bacteriological examination of milk sample revealed 187 isolates where coagulase negative Staphylococcus species (CNS) (34.2%) was the predominant species while Streptococcus faecalis (2.1%) was identified as the least bacteria. The present study concluded that prevalence of mastitis particularly the subclinical mastitis was major problem of dairy cows in the area and hence warrants serious attention.

scholarly journals Microbiological analysis and sensitivity profile of Staphylococcus spp. in subclinical mastitis of dairy goats

2016 ◽  
Vol 68 (2) ◽  
pp. 336-344
Author(s):  
S.R.S. Salaberry ◽  
A.B.S. Saidenberg ◽  
E. Zuniga ◽  
F.F. Gonsales ◽  
P.A. Melville ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Economic Losses ◽  
Microbiological Analysis ◽  
Dairy Goats ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Resistant Strains ◽  
Staphylococcus Spp ◽  
Sensitivity Profile

Subclinical mastitis in goats causes economic losses and risks to public health. Given the need for research that shows the most isolated staphylococci species and sensibility tests comparing the resistance between coagulase-negative (CNS) and positive Staphylococcus (CPS) goats with subclinical mastitis, the aim of this study was to identify the microorganisms isolated from milk samples of goats with subclinical mastitis, as well as define the staphylococci species and determine the sensitivity profile of Staphylococcus spp. to antimicrobials. To collect samples, tests were performed for mug of black background and California mastitis test (CMT), collecting milk from CMT positive animals. A total of 226 samples from seven herds of dairy goats was collected and forwarded to the laboratory, where they were seeded for the isolation of the microorganism and implementing the antibiotic sensibility test. Of these, 122 samples had bacterial growth and the most isolated staphylococci species were: S. epidermidis (24.55%), S. lugdunensis (15.40%) and S. intermedius (13.64%). Samples showed increased resistance to antimicrobials: penicillin (81.8%), oxacillin (60.0%) and ampicillin (55.5%). Greater sensitivity to: enrofloxacin (99.1%), erythromycin (98.2%), gentamicin (98.2%) and vancomycin (98.2%) were observed. The S. epidermidis showed higher antimicrobial resistance to amoxicillin and penicillin than S. lugdunensis and S. intermedius. Similar resistance in vitro between CNS and CPS was observed to most antimicrobials. It is important to control the overuse of antibiotics to prevent the emergence of resistant strains.

scholarly journals PREVALENCE OF SUBCLINICAL MASTITIS IN DAIRY COWS IN SELECTED AREAS OF BANGLADESH

2012 ◽  
Vol 9 (1) ◽  
pp. 73-78 ◽  
Author(s):  
MA Islam ◽  
MZ Islam ◽  
MA Islam ◽  
MS Rahman ◽  
MT Islam
Keyword(s):  
Dairy Cows ◽  
Rural Areas ◽  
Dairy Farm ◽  
Local Area ◽  
Subclinical Mastitis ◽  
Local Breed ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Late Stages

This research was carried out to determine the prevalence of subclinical mastitis in lactating Dairy Cow of Bangladesh Agricultural University dairy farm (BAUDF) and rural areas of Tangail sadar upazila of Bangladesh during the period of July 2009 to April 2010. A total of 200 milk samples (40 from BAUDF and 160 from Tangail sadar upazila) were collected for this study which were subjected to physical examination and subsequently screened for subclinical mastitis using three indirect tests viz. White Side Test (WST), California Mastitis Test (CMT), and Surf Field Mastitis Test (SFMT). Overall prevalence of subclinical mastitis (SCM) in lactating dairy cows found in this study was 29%. Cows were infected with SCM 29.5%, 27.5% and 25.5% detection by CMT, WST and SFMT respectively. Higher prevalence of SCM was detected in milch crossbred cows (36.36%) in comparison to local bred cows (24.61%) maintained under extensive management system in Rural area of Tangail sadar upazila. The prevalence of SCM was recorded in 31.58%, 30.76% and 68.75% in cows of local area of Tangail sadar upazila, and 25.0%, 40.0% and 71.42% in cows of BAU,DF during the early, mid and late stages of lactation respectively. The highest prevalence of SCM was recorded during the early lactation stage in both the local breed cows (30.0%) and cows of BAUDF (45.83%) in comparison to their respective mid and late stages of lactation. The prevalence of SCM was highest in lactating cows having third lactation and high yielding (cows produced >10 liter milk per day) both in local breed and crossbred cows.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11216 Bangl. J. Vet. Med. (2011). 9(1): 73-78 

scholarly journals The Impact of Mastitis on Reproductive Parameters of Dairy Cows

2019 ◽  
Vol 63 (3) ◽  
pp. 72-78
Author(s):  
F. Zigo ◽  
A. Adamová ◽  
M. Vasiľ ◽  
J. Elečko ◽  
S. Ondrašovičová ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reproductive Parameters ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Service Period ◽  
High Incidence ◽  
The Impact

Abstract The aim of this study was to evaluate the occurrence of mastitis and its impact on the reproductive parameters in a herd of 180 dairy cows. Based on the herd records 127 cows of Slovak spotted cattle and their crosses with red Holstein were selected for study between 1—2 months after calving. The examination of the health status of the mammary glands consisted of: the clinical examination of the udder, the California mastitis test (CMT) supplemented by the collection of mixed milk samples, and the laboratory examination of bacterial pathogens causing the mastitis. In addition to the mam­mary investigation, reproduction indicators such as the length of the insemination interval, the service period, the intercalving period and the insemination index were also analyzed. The results of this study indicated: a high incidence of mastitis (41.6 %), especially latent (21.2 %), subclinical (15.7 %) and clinical (4.7 %) forms were most common in the herd. The most frequently isolated bacteria from the infected milk samples were: coagulase negative staphylococci (54.1 %), S. aureus (16.9 %), Streptococcus spp. (15.0 %), A. viridans (7.5 %) and Ent. faecalis (6.4 %). According to the available literature, the optimum values of the intercalving period were 365—400 days, the insemination interval 55—80 days, the insemination index 1.2—2 and the service period 60—110 days. In comparison, our results showed increased, unsatisfactory reproductive values in the group of dairy cows with clinical mastitis. While in healthy cows as well as in groups of cows with latent and subclinical mastitis, all of the reproductive indicators were within the optimal levels.

Subclinical mastitis in dairy cows in Swiss organic and conventional production systems

2006 ◽  
Vol 74 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Markus Roesch ◽  
Marcus G Doherr ◽  
Walter Schären ◽  
Melchior Schällibaum ◽  
Jürg W Blum
Keyword(s):  
Dairy Cows ◽  
Production Systems ◽  
Post Partum ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Cell Counts ◽  
Conventional Production

The objective was to compare the prevalence of subclinical mastitis (SM) and of udder pathogens in 60 Swiss organic (OP) and 60 conventional production systems (CP). Cows (n=970) were studied for SM prevalence and udder pathogens at median 31 d and 102 d post partum. Cows showing a [ges ]1+ positive California Mastitis Test (CMT) in at least one quarter were considered to have SM. Cow-level prevalences of SM for visits at 31 d and 102 d post partum (39% and 40% in OP and 34% and 35% in CP) were similar, but quarter-level prevalences of SM were higher (P<0·02) in OP than CP (15% and 18% in OP and 12% and 15% in CP). Median somatic cell counts in milk at 31 d post partum were higher (P<0·05) in OP than CP cows (43000 and 28000 cells/ml, respectively), but were similar at 102 d post partum in OP and CP cows (45000 and 38000 cells/ml, respectively). In milk samples from quarters showing a CMT reaction [ges ]2+ the prevalences of coagulase negative staphylococci were lower (P<0·05) at 102 d post partum, whereas prevalences of non-agalactiae streptococci were higher (P<0·05) in OP than in CP cows at 31 d and 102 d post partum. In conclusion, under Swiss conditions, subclinical mastitis is a greater problem in organic than in conventional production systems, but differences are not marked.

scholarly journals Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

2015 ◽  
Vol 65 (3) ◽  
pp. 358-370 ◽  
Author(s):  
Gürler Hande ◽  
Findik Arzu ◽  
Gültiken Nilgün ◽  
Ay Serhan Serhat ◽  
Çiftçi Alper ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Culture Methods ◽  
Milk Samples ◽  
California Mastitis Test ◽  
High Prevalence ◽  
Enterococcus Spp ◽  
Molecular Identification Methods ◽  
Polymerase Chain ◽  
Jersey Cows

AbstractThe aim of this study was to investigate the etiology of subclinical mastitis (SCM) in dairy Jersey cows with the use of bacteriological and molecular identification methods. In the study 121 Jersey and 78 hybrid Jersey cows with SCM were observed in the Samsun district of Turkey. A total of 411 California mastitis test (CMT) positive milk samples from these animals were examined bacteriologically. The prevalence of subclinical mastitis was 54.75% and 67.2% in Jerseys and hybrids, respectively. On bacteriological examination, a total of 92 strains were isolated from 411 milk samples. The most prevalent bacteria wereStaphylococcusspp. (69.56%). Among them 24 isolates wereStaphylococcus aureus(26.08%) the other isolates wereStreptococcus dysgalactiae(23.91%),Enterococcus spp.(3.26%) andStreptococcus agalactiae(3.26%). All strains were identified with bacteriological culture methods, as well as by Polymerase Chain Reaction (PCR). Gram-negative bacteria were not isolated. In conclusion, the etiology of SCM in full blood and hybrid Jersey dairy cows in Samsun and the prevalence of bacteria were determined. The relatively high prevalence of SCM indicates the potential need for the consideration of some factors contributing to the formation of mastitis (e.g. management) as well as bacterial agents. The present study and further studies may be useful to develop mastitis vaccines by means of providing true vaccine strain sources.

scholarly journals Comparison of cow-side diagnostic tests for subclinical mastitis of dairy cows in Musanze district, Rwanda

2017 ◽  
Vol 88 ◽  
Author(s):  
Blaise Iraguha ◽  
Humphrey Hamudikuwanda ◽  
Borden Mushonga ◽  
Erick Kandiwa ◽  
Jean P. Mpatswenumugabo
Keyword(s):  
Dairy Cows ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Subclinical Mastitis ◽  
Kappa Statistics ◽  
Test Results ◽  
Substantial Agreement ◽  
Likelihood Ratios ◽  
California Mastitis Test ◽  
Strength Of Association

Four subclinical mastitis diagnostic tests (the UdderCheck® test [a lactate dehydrogenasebased test], the California Mastitis Test [CMT], the Draminski® test [a conductivity-based test] and the PortaSCC® test [a portable somatic cell count-based test]) were compared in a study comprising crossbreed dairy cows (n = 30) during September and October 2015. Sensitivity and specificity of the CMT, Draminski® and UdderCheck® tests were compared with the PortaSCC® as reference. The CMT, Draminski® and UdderCheck® test results were compared with the results of the PortaSCC® test using kappa statistics. Duplicate quarter milk samples (n = 120) were concurrently subjected to the four tests. Sensitivity and specificity were 88.46% and 86.17% (CMT), 78.5% and 81.4% (Draminski®) and 64.00% and 78.95% (UdderCheck®). The CMT showed substantial agreement (k = 0.66), the Draminski® test showed moderate agreement (k = 0.48) and the UdderCheck® test showed fair agreement (k = 0.37) with the PortaSCC® test and positive likelihood ratios were 6.40, 4.15 and 3.04, respectively. The cow-level subclinical mastitis prevalence was 70%, 60%, 60% and 56.7% for PortaSCC®, CMT, Draminski® and UdderCheck® tests, respectively. At udder quarter level, subclinical mastitis prevalence was 20%, 21.67% and 20.83% for PortaSCC®, CMT and UdderCheck®, respectively. A correlation (P < 0.05) and moderate strength of association were found between the four tests used. The study showed that compared to the PortaSCC® test, the CMT was the most preferable option, followed by the Draminski® test, while the UdderCheck® test was the least preferable option for subclinical mastitis screening.

SUBCLINICAL MASTITIS IN LACTATING COWS: COMPARISON OF FOUR SCREENING TESTS AND EFFECT OF ANIMAL FACTORS ON ITS OCCURRENCE

2016 ◽  
Vol 13 (2) ◽  
pp. 41-50 ◽  
Author(s):  
M Badiuzzaman ◽  
MA Samad ◽  
SHMF Siddiki ◽  
MT Islam ◽  
S Saha
Keyword(s):  
Dairy Cows ◽  
Predictive Value ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Standard Test ◽  
Screening Tests ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Cultural Isolation

A total of 444 quarter milk samples from 111 crossbred dairy cows were subjected to California mastitis test (CMT), somatic cell count (SCC) test, white side test (WST) and surf field mastitis (SFMT) test to quantify their efficacy in detecting sub clinical mastitis in dairy cows of Bangladesh during the period from 2010 to 2011.Milk samples positive by CMT, SCC, WST and SFMT were 265,230, 225 and 222 respectively. All samples were subjected to cultural isolation (gold standard test for comparison of indirect mastitis tests). Of these, 261 samples were positive by cultural isolation. The sensitivity of the CMT, SCC, WST and SFMT were 80.08%, 86.60%, 60.54% and 57.47%; specificity 69.40%, 97.81%, 63.38% and 60.66%; percentage accuracy 75.68%,91.22%, 61.71%  and 58.78%; positive predictive value 78.87%, 98.26%, 70.22% and 67.57%; and negative predictive value 70.95%, 83.64%, 52.97% and 50%respectively. Quarter-wise sub clinical mastitis (SCM) was detected in 59.68%, 51.80%, 50.68% and 50% samples by CMT, SCC, WST and SFMT, respectively, while animal-wise SCM was recorded in 72.07%, 66.67%, 64.86% and 61.26% samples by CMT, SCC, WST and SFMT, respectively. The right hind quarters were most significantly (p<0.001) susceptible to SCM than other quarters. Cows with 3rd and 4th parity and at their early lactation stage had significantly higher (p<0.001) SCM than others. Kappa value of SCC was higher than that of CMT. CMT was concluded to be the most accurate test after cultural isolation and SCC. Unlike laboratory tests as cultural isolation and SCC that require adequate laboratory facilities and skilled personnel, CMT is a reliable diagnostic method in field conditions.

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