Design a Biologically Inspired Nanostructured Coating for Better Osseointegration

2012 ◽  
Vol 1418 ◽  
Author(s):  
Mian Wang ◽  
Jian Li ◽  
Michael Keidar ◽  
Lijie Grace Zhang
Keyword(s):  
Cell Adhesion ◽  
Magnetic Fields ◽  
Bone Matrix ◽  
Orthopedic Implants ◽  
Nanostructured Coating ◽  
Single Wall Carbon Nanotubes ◽  
Great Promise ◽  
Biologically Inspired ◽  
Number Of Patients

ABSTRACTTo date, there are a strikingly growing number of patients who need various orthopedic implants. However, traditional orthopedic implants face many complications such as infection and implant loosening which may lead to implant failures. Conventional metal implants such as titanium were chosen for orthopedic applications mainly based on their excellent mechanical properties and biological inertness. Since natural bone matrix is nanometer in dimension, it is desirable to design a biologically inspired nanostructured coating that can turn conventional inert titanium surfaces into biomimetic active interfaces, thus enhance bone cell adhesion and osseointegration. For this purpose, we designed a biomimetic nanostructured coating based on nanocrystalline hydroxyapatites (nHA) and single wall carbon nanotubes (SWCNTs). Specifically, nHA with good crystallinity and biomimetic dimensions were prepared via a wet chemistry method and hydrothermal treatment; and the SWCNTs were synthesized via an arc plasma method with or without magnetic fields. TEM images showed that the hydrothermally treated nHA possessed regular rod-like nanocrystals and biomimetic nanostructure. In addition, the length of SWCNTs can be significantly increased under external magnetic fields when compared to nanotubes produced without magnetic fields. More importantly, our results showed that the above nHA and SWCNTs nanomaterials can greatly promote osteoblast (bone-forming cell) adhesion on titanium in vitro, thus holding great promise to improve osseointegration and lengthen the lifetime of current orthopedic implants.

scholarly journals Multifunctional Properties of Quercitrin-Coated Porous Ti-6Al-4V Implants for Orthopaedic Applications Assessed In Vitro

2020 ◽  
Vol 9 (3) ◽  
pp. 855 ◽  
Author(s):  
Maria Antonia Llopis-Grimalt ◽  
Aina Arbós ◽  
Maria Gil-Mir ◽  
Aleksandra Mosur ◽  
Prathamesh Kulkarni ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Antibacterial Properties ◽  
Young Modulus ◽  
Orthopedic Implants ◽  
Antibacterial Activities ◽  
Bone Cell ◽  
Alp Activity ◽  
Porous Ti ◽  
Osteocalcin Production

(1) One strategy to improve the outcome of orthopedic implants is to use porous implants with the addition of a coating with an antibacterial biomolecule. In this study, we aimed to produce and test the biocompatibility, the osteopromotive (both under normal conditions and under a bacterial challenge with lipopolysaccharide (LPS)) and antibacterial activities of a porous Ti-6Al-4V implant coated with the flavonoid quercitrin in vitro. (2) Porous Ti-6Al-4V implants were produced by 3D printing and further functionalized with quercitrin by wet chemistry. Implants were characterized in terms of porosity and mechanical testing, and the coating with quercitrin by fluorescence staining. Implant biocompatibility and bioactivity was tested using MC3T3-E1 preosteoblasts by analyzing cytotoxicity, cell adhesion, osteocalcin production, and alkaline phosphatase (ALP) activity under control and under bacterial challenging conditions using lipopolysaccharide (LPS). Finally, the antibacterial properties of the implants were studied using Staphylococcus epidermidis by measuring bacterial viability and adhesion. (3) Porous implants showed pore size of about 500 µm and a porosity of 52%. The coating was homogeneous over all the 3D surface and did not alter the mechanical properties of the Young modulus. Quercitrin-coated implants showed higher biocompatibility, cell adhesion, and osteocalcin production compared with control implants. Moreover, higher ALP activity was observed for the quercitrin group under both normal and bacterial challenging conditions. Finally, S. epidermidis live/dead ratio and adhesion after 4 h of incubation was lower on quercitrin implants compared with the control. (4) Quercitrin-functionalized porous Ti-6Al-4V implants present a great potential as an orthopedic porous implant that decreases bacterial adhesion and viability while promoting bone cell growth and differentiation.

Different Cell Responses on Biologically Inspired Nano-coatings for Orthopedic Applications

2009 ◽  
Vol 1209 ◽  
Author(s):  
Lijie Zhang ◽  
Usha D. Hemraz ◽  
Hicham Fenniri ◽  
Thomas J Webster
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Surface Chemistry ◽  
In Vitro Study ◽  
Clinical Problem ◽  
Biologically Inspired ◽  
Age Related ◽  
Orthopedic Applications ◽  
Endothelial Cell Adhesion

AbstractVarious bone defects, caused by trauma, disease or age-related degeneration, represent a crucial clinical problem all over the world. However, traditional implant materials may cause many complications after surgeries, leading to intense patient pain. Thus, the objective of this in vitro study was to develop a biologically inspired coating on conventional titanium with materials that possess biomimetic nanostructured architectures and favorable surface chemistry. Specifically, self-assembled rosette nanotubes (RNTs) functionalized with various osteogenic peptides and amino acids (such as lysine-arginine-serine-arginine (KRSR), arginine-glycine-aspartic acid (RGD) and lysine (K)) were designed as coatings. Results revealed excellent cytocompatibility properties of these RNTs towards enhancing osteoblast (bone forming cell) and endothelial cell adhesion. In particular, KRSR and RGD functionalized RNTs coated on titanium promoted the greatest osteoblast densities when compared to uncoated titanium. In addition, the KRSR functionalized RNTs selectively improved osteoblast adhesion but not endothelial cell adhesion when coated on titanium. From this study, it can be speculated that the biologically inspired nanotubular structure and osteogenic surface chemistry of RNTs altered the surface properties of titanium to transform it into a more favorable environment for orthopedic applications.

scholarly journals Tantalum Nanoparticles Reinforced PCL Scaffolds Using Direct 3D Printing for Bone Tissue Engineering

2021 ◽  
Vol 8 ◽  
Author(s):  
Zixuan Xiong ◽  
Wenbin Liu ◽  
Hu Qian ◽  
Ting Lei ◽  
Xi He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Biological Activity ◽  
Cell Adhesion ◽  
3D Printing ◽  
Orthopedic Implants ◽  
Biological Properties ◽  
Related Gene ◽  
Clinical Potential

Polycarbonate (PCL) has been widely used in tissue engineering, but its hydrophobicity and low biological activity limit its further promotion and application. By adding nanoparticles, the hydrophilicity and biological activity of PCL can be improved. In this study, different amounts of Ta (1–10%wt) were added to PCL, and then their mechanical and biological properties were studied in vitro. XRD found that 5%Ta-PCL has the highest crystallinity. At the same time, cell experiments CCK8, cell adhesion, osteogenic differentiation, and osteogenesis related gene expression showed that Ta can enhance the mechanical and biological properties of PCL, while 5% Ta-PCL showed the best mechanical and biological properties. This composite of tantalum and PCL could have a clinical potential for orthopedic implants.

Study on Degradation Behavior and Biocompatibility of Polymethyl Methacrylate/Mineralized Collagen/Mg–Ca Alloy Composite Material

2020 ◽  
Vol 10 (2) ◽  
pp. 139-150
Author(s):  
Yuan-Xin Shen ◽  
Xi-Rao Sun ◽  
Cheng-Yue Wang ◽  
Jing-Xin Yang ◽  
Jia-Xin Bao
Keyword(s):  
Weight Loss ◽  
Alkaline Phosphatase ◽  
Cell Adhesion ◽  
Orthopedic Implants ◽  
Dip Coating ◽  
Alizarin Red ◽  
Pmma Bone Cement ◽  
Degradation Test ◽  
Mineralized Collagen

In this study, we composited mineralized collagen and magnesium-calcium alloy by freeze-drying, followed by dip-coating PMMA bone cement to enhance the composite of mineralized collagen and magnesium-calcium alloy. In vitro degradation test was performed to observe the pH and weight loss of the material. The contact angle test was used to detect the hydrophilicity of the material. Subsequently, MC3T3-E1 were used to assess cell biocompatibility In vitro by cell adhesion, cytotoxicity, alkaline phosphatase, alizarin red staining, and cytoskeleton. The results showed that the pH changes of the PMMA/NHAC/Mg–Ca was slower than that of the Mg–Ca , and the weight loss rate at 7 d and 14 d were lower than that of the Mg–Ca (P < 0.05) in degradation test. Wettability experiment showed that PMMA/NHAC/Mg–Ca was a hydrophilic material and Mg–Ca was a hydrophobic material (P < 0.05). In vitro cell experiments, the PMMA/NHAC/Mg–Ca had more cell adhesion than Mg–Ca and more synapses were connected to others. In the cytotoxicity experiment, the cell proliferation lever of PMMA/NHAC/Mg–Ca was higher than that of Mg–Ca at each time point (P < 0.05). In the 7 d alkaline phosphatase experiment, the PMMA/NHAC/Mg–Ca showed higher ALP activity than the Mg–Ca (P < 0.05), and in the alizarin red experiment at 14 d and 28 d, there were more obvious calcified nodules and mineralized area. After 1 day of culture in the PMMA/NHAC/Mg–Ca extract, the cells showed a clearer and more complete cytoskeletal structure and better cell morphology. In conclusion, PMMA/NHAC/Mg–Ca orthopedic implants had a better hydrophilicity, cytotoxicity and osteogenic ability, besides with a slower rate of degradation, and could be implanted in animals for further research, which were expected to be used for the repair of clinical bone defects.

Binding to Secreted Bone Matrix in vitro

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (4) ◽  
Author(s):  
Aurélie Tormo ◽  
Christian Beauséjour ◽  
Jean-François Gauchat
Keyword(s):  
Bone Matrix

scholarly journals CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells

2021 ◽  
Author(s):  
Mattias Lepsenyi ◽  
Nader Algethami ◽  
Amr A. Al-Haidari ◽  
Anwar Algaber ◽  
Ingvar Syk ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Adhesion ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Colon Cancer Cells ◽  
Cancer Cell Adhesion ◽  
And Migration ◽  
Cxcr2 Antagonist

AbstractPeritoneal metastasis is an insidious aspect of colorectal cancer. The aim of the present study was to define mechanisms regulating colon cancer cell adhesion and spread to peritoneal wounds after abdominal surgery. Mice was laparotomized and injected intraperitoneally with CT-26 colon carcinoma cells and metastatic noduli in the peritoneal cavity was quantified after treatment with a CXCR2 antagonist or integrin-αV-antibody. CT-26 cells expressed cell surface chemokine receptors CXCR2, CXCR3, CXCR4 and CXCR5. Stimulation with the CXCR2 ligand, CXCL2, dose-dependently increased proliferation and migration of CT-26 cells in vitro. The CXCR2 antagonist, SB225002, dose-dependently decreased CXCL2-induced proliferation and migration of colon cancer cells in vitro. Intraperitoneal administration of CT-26 colon cancer cells resulted in wide-spread growth of metastatic nodules at the peritoneal surface of laparotomized animals. Laparotomy increased gene expression of CXCL2 at the incisional line. Pretreatment with CXCR2 antagonist reduced metastatic nodules by 70%. Moreover, stimulation with CXCL2 increased CT-26 cell adhesion to extracellular matrix (ECM) proteins in a CXCR2-dependent manner. CT-26 cells expressed the αV, β1 and β3 integrin subunits and immunoneutralization of αV abolished CXCL2-triggered adhesion of CT-26 to vitronectin, fibronectin and fibrinogen. Finally, inhibition of the αV integrin significantly attenuated the number of carcinomatosis nodules by 69% in laparotomized mice. These results were validated by use of the human colon cancer cell line HT-29 in vitro. Our data show that colon cancer cell adhesion and growth on peritoneal wound sites is mediated by a CXCL2-CXCR2 signaling axis and αV integrin-dependent adhesion to ECM proteins.

scholarly journals High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhou Fang ◽  
Junjian Chen ◽  
Ye Zhu ◽  
Guansong Hu ◽  
Haoqian Xin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Rational Design ◽  
Click Reaction ◽  
Reaction Times ◽  
Great Promise ◽  
Biomaterial Surfaces

AbstractPeptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-throughput strategy, preparing titanium (Ti) surfaces with a gradient in peptide density by click reaction as a platform, to screen the positions with desired functions. Such positions are corresponding to optimized molecular parameters (peptide densities/ratios) and associated preparation parameters (reaction times/reactant concentrations). These parameters are then extracted to prepare nongradient mono- and dual-peptide functionalized Ti surfaces with desired biocompatibility or/and antimicrobial activity in vitro and in vivo. We also demonstrate this strategy could be extended to other materials. Here, we show that the high-throughput versatile strategy holds great promise for rational design and preparation of functional biomaterial surfaces.

scholarly journals Glycan-to-Glycan Binding: Molecular Recognition through Polyvalent Interactions Mediates Specific Cell Adhesion

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 397
Author(s):  
Gradimir Misevic ◽  
Emanuela Garbarino
Keyword(s):  
Cell Adhesion ◽  
Plasma Membranes ◽  
Specific Cell ◽  
Cellular Interactions ◽  
New Paradigm ◽  
Tumor Cell Adhesion ◽  
Self Recognition ◽  
Structural And Functional Properties ◽  
Low Valent

Glycan-to-glycan binding was shown by biochemical and biophysical measurements to mediate xenogeneic self-recognition and adhesion in sponges, stage-specific cell compaction in mice embryos, and in vitro tumor cell adhesion in mammals. This intermolecular recognition process is accepted as the new paradigm accompanying high-affinity and low valent protein-to-protein and protein-to-glycan binding in cellular interactions. Glycan structures in sponges have novel species-specific sequences. Their common features are the large size >100 kD, polyvalency >100 repeats of the specific self-binding oligosaccharide, the presence of fucose, and sulfated and/or pyruvylated hexoses. These structural and functional properties, different from glycosaminoglycans, inspired their classification under the glyconectin name. The molecular mechanism underlying homophilic glyconectin-to-glyconectin binding relies on highly polyvalent, strong, and structure-specific interactions of small oligosaccharide motifs, possessing ultra-weak self-binding strength and affinity. Glyconectin localization at the glycocalyx outermost cell surface layer suggests their role in the initial recognition and adhesion event during the complex and multistep process. In mammals, Lex-to-Lex homophilic binding is structure-specific and has ultra-weak affinity. Cell adhesion is achieved through highly polyvalent interactions, enabled by clustering of small low valent structure in plasma membranes.

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