KAJIAN EFEKTIVITAS KALSIUM UNTUK PENGEMBANGAN TEKNOLOGI INTENSIF PADA BUDIDAYA LOBSTER AIR TAWAR (Cherax quadricarinatus)

Dalam proses pertumbuhan lobster dibutuhkan mineral kalsium (Ca) yang dapat dicukupi dari makanan dan lingkungan. Pada fase pengerasan kulit diperlukan kalsium yang cukup tinggi. Kebutuhan akan kalsium tidak dapat dipenuhi dari dalam tubuhnya yang tersimpan di dalam hemolimf. Oleh karena itu, diperlukan kajian tentang efektivitas mineral kalsium di dalam air pada budidaya lobster. Penelitian dilakukan secara in door pada bak semen yang dilengkapi dengan sistem resirkulasi. Rancangan penelitian dilakukan dengan menggunakan Rancangan Acak Lengkap dengan 4 perlakuan konsentrasi penambahan calcium hydroxyde ke dalam media yaitu 0 mg/L, 5 mg/L, 10 mg/L, dan 15 mg/L. Masing-masing perlakuan mendapat 3 ulangan. Hasil penelitian menunjukkan bahwa efek pemberian kalsium (CaOH2) pada lobster tawar terlihat secara signifikan memberikan efek yang positif dan jauh lebih baik dibandingkan dengan kontrol (P<0,05). Konsentrasi kalsium 5,0–10,0 mg/L memberikan hasil yang baik dalam mempengaruhi pertumbuhan lobster. Hasil yang diperoleh dari penelitian ini membuktikan bahwa manajemen dalam konsentrasi kalsium yang seimbang pada media pemeliharaan lobster dapat diaplikasikan sehingga budidaya lobster dapat dilaksanakan secara intensif.Calcium mineral was need to support of lobster growth it’s sufficient from feed and environment. Phase of harden carapace was need a high calcium. The calcium need did not fulfilled by hemolymphe body. And then the assessment of calcium effect was conducted to lobster culture. Complete randomized design was used for this research with different level of calcium hydroxide to the media namely 0.0 mg/L, 5.0 mg/L, 10.0 mg/L, and 15.0 mg/L. The experiment was conducted in the completely randomized design with three replications. Result of this experiment showed that there were significant different positively compared than control (P<0.05). Calcium level of 5.0-10.0 mg/L indicated positively affect to the growth of lobster. Result of this experiment to prove that management of calcium in the media rearing can be used to intensive culture of lobster.

Download Full-text

Isolation and Characterization of Yeasts from Rumen Fluids for Potential Use as Additives in Ruminant Feeding

Veterinary Sciences ◽

10.3390/vetsci8030052 ◽

2021 ◽

Vol 8 (3) ◽

pp. 52

Author(s):

Chanon Suntara ◽

Anusorn Cherdthong ◽

Metha Wanapat ◽

Suthipong Uriyapongson ◽

Vichai Leelavatcharamas ◽

...

Keyword(s):

Incubation Time ◽

Cellulase Activity ◽

The Other ◽

Pichia Kudriavzevii ◽

Factor B ◽

Yeast Strains ◽

Randomized Design ◽

Holstein Friesian ◽

The Media ◽

Completely Randomized Design

Saccharomyces cerevisiae is a yeast strain often used to improve the feed quality of ruminants. However, S. cerevisiae has limited capacity to provide biomass when inoculated with carbon sources and a low ability to produce cellulase enzymes. Here, we hypothesized that yeast in the rumen produces a large amount of biomass and could release cellulase enzymes to break down fiber content. Therefore, the aim of this study was to screen, isolate and identify yeast from the rumen fluids of Holstein Friesian steers and measure the efficiency of biomass production and cellulase activity. A fermentation medium containing sugarcane molasses as a carbon source and urea as a nitrogen source was optimized. Two fistulated–crossbred Holstein Friesian steers averaging 350 ± 20 kg body weight were used to screen and isolate the ruminal yeast. Two experiments were designed: First, a 12 × 3 × 3 factorial was used in a completely randomized design to determine biomass and carboxymethyl cellulase activity. Factor A was the isolated yeast and S. cerevisiae. Factor B was sugarcane molasses (M) concentration. Factor C was urea (U) concentration. In the second experiment, potential yeasts were selected, identified, and analyzed for 7 × 4 factorial use in a completely randomized design. Factor A was the incubation times. Factor B was the isolated yeast strains, including codes H-Khon Kaen University (KKU) 20 (as P. kudriavzevii-KKU20), I-KKU20 (C. tropicalis-KKU20), and C-KKU20 (as Galactomyces sp.-KKU20). Isolation was imposed under aerobic conditions, resulting in a total of 11 different colonies. Two appearances of colonies including asymmetric colonies of isolated yeast (indicated as A, B, C, E, and J) and ovoid colonies (coded as D, F, G, H, I, and K) were noted. Isolated yeast from the rumen capable of providing a high amount of biomass when inoculant consisted of the molasses 15% + urea 3% (M15 + U3), molasses 25% + urea 1% (M25 + U1), molasses 25% + urea 3% (M25 + U3), and molasses 25% + urea 5% (M25 + U5) when compared to the other media solution (p < 0.01). In addition, 11 isolated biomass-producing yeasts were found in the media solution of M25 + U1. There were 4 isolates cellulase producing yeasts discovered in the media solution of M25 + U1 and M25 + U5 whereas molasses 5% + urea 1% (M5 + U1), molasses 5% + urea 3% (M5 + U3), molasses 5% + urea 5% (M5 + U5), molasses 15% + urea 1% (M15 + U1), molasses 15% + urea 3% (M5 + U3), and M25 + U3 were found with 2, 3, 1, 2, 1, and 2 isolates, respectively. Ruminal yeast strains H-KKU20, I-KKU20, and C-KKU20 were selected for their ability to produce biomass. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20 while C-KKU20 was identified as Galactomyces sp.-KKU20. Two strains provided maximum cell growth: P. kudriavzevii-KKU20 (9.78 and 10.02 Log cell/mL) and C. tropicalis-KKU20 (9.53 and 9.6 Log cells/mL) at 60 and 72 h of incubation time, respectively. The highest ethanol production was observed in S. cerevisiae at 76.4, 77.8, 78.5, and 78.6 g/L at 36, 48, 60, and 72 h of incubation time, respectively (p < 0.01). The P. kudriavzevii-KKU20 yielded the least reducing sugar at about 30.6 and 29.8 g/L at 60 and 72 h of incubation time, respectively. The screening and isolation of yeasts from rumen fluids resulted in 11 different yeasts being obtained. The potential yeasts discovered in the rumen fluid of cattle were Pichia kudriavzevii-KKU20, Candida tropicalis-KKU20, and Galactomyces sp.-KKU20. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of biomass production, cellulase enzyme activity, and cell number.

Download Full-text

Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2020.92.109-115 ◽

2021 ◽

Vol 9 (2) ◽

pp. 109-115

Author(s):

Ahmad Saifun Naser ◽

Muhammad Wisnu

Keyword(s):

Tissue Culture ◽

Callus Formation ◽

Leaf Explants ◽

Chrysanthemum Morifolium ◽

Stem Explants ◽

Appearance Time ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

Chrysanthemum Morifolium Ramat

Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.

Download Full-text

Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v16i1.18908 ◽

2014 ◽

Vol 16 (1) ◽

pp. 19

Author(s):

Samanhudi Samanhudi ◽

Dwi Hardjoko ◽

Riandy Adhitya

Keyword(s):

Wood Fiber ◽

Dry Weight ◽

Fresh Weight ◽

Randomized Design ◽

The Media ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Brassica Oleraceae ◽

Media Mix ◽

Leaf Greenness

<p>Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk</p>

Download Full-text

EFEKTIVITAS PEMBERIAN HARA MIKRO MELALUI MEDIA DAN DAUN PADA TANAMAN KANGKUNG (Ipomea reptans Poir)

Agrin ◽

10.20884/1.agrin.2018.22.1.457 ◽

2019 ◽

Vol 22 (1) ◽

pp. 39

Author(s):

Bhaskara Anggarda Gathot Subrata ◽

Stefany Darsan

Keyword(s):

Leaf Area ◽

Rate Ratio ◽

Growth Analysis ◽

Dry Weight ◽

Growth And Yield ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design

Penelitian bertujuan untuk mengetahui efektivitas pemberian hara mikro melalui akar dan daun. Penelitiandilaksanakan dengan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal. Perlakuan berupa kontrol,pupuk mikro lewat media, pupuk mikro lewat daun. Masing-masing perlakuan diulang 6 kali. Media tanam berupapasir yang sudah dimasukan ke dalam bak penanaman, setelah itu tanam benih kangkung dengan jarak 10x10 cmpada setiap bak. Lalu aplikasikan pupuk melalui media atau daun seminggu sekali. Pengamatan yang dilakukanmeliputi tinggi tanaman dan kehijauan daun dengan SPAD 502 untuk tiap minggunya; aktivitas nitrate reduktase,kandungan klorofil a, klorofil b, dan klorofil total, bobot kering batang, bobot kering tajuk, bobot kering daun,bobot kering akar, luas daun (LD), serta analisis pertumbuhan tanaman, meliputi nisbah laju daun (NLD), luasdaun khas (LDK), bobot daun khas (BDK). Berdasarkan penelitian yang telah dilakukan, pemberian pupuk cairmelalui media maupun daun belum mampu meningkatkan pertumbuhan dan hasil tanaman kangkung. Perlakuanpada daun cenderung menunjukan hasil yang lebih tinggi dibandingkan perlakuan pada media pada semuaparameter pengamatan.Kata kunci: efektivitas, hara mikro, akar dan daunABSTRACTThe aim of this research is to know the effectiveness of micro nutrient giving through root and leaf. Theresearch was conducted by using Completely Randomized Design (CRD) of single factor. Treatment of control,micro-fertilizer through the media, micro-fertilizer through the leaves. Each treatment was repeated 6 times.Planting media in the form of sand that has been inserted into the tub of planting, after that plant the seeds ofkangkung with a distance of 10x10 cm in each tub. Then apply the fertilizer through the media or leaves once aweek. Observations included plant height and greenery of leaves with SPAD 502 for each week; nitrate reductaseactivity, chlorophyll a, chlorophyll b, and total chlorophyll, dry weight of stalk, dry weight of canopy, leaf dryweight, root dry weight, leaf area, and plant growth analysis, including leaf rate ratio, typical leaf area, typicalleaf weight. Based on the research that has been done, the application of liquid fertilizer through the media andleaves has not been to increase the growth and yield of kangkung. Treatment on the leaves tend to show higherresults than treatment on media at all observation parameter.Keywords: effectiveness, micro nutrients, roots and leaves

Download Full-text

Kandungan Klorofil Total Daun Pada Padi Lokal Sulawesi Utara Yang Mengalami Cekaman Banjir

Jurnal MIPA ◽

10.35799/jm.8.2.2019.23516 ◽

2019 ◽

Vol 8 (2) ◽

pp. 51

Author(s):

Fitria Tjolleng ◽

Parluhutan Siahaan ◽

Nio Song Ai

Keyword(s):

Chlorophyll Content ◽

Physiological Responses ◽

Time Factors ◽

Total Chlorophyll ◽

Total Chlorophyll Content ◽

North Sulawesi ◽

Randomized Design ◽

The Media ◽

Rice Leaves ◽

Completely Randomized Design

Penelitian ini bertujuan untuk mengkaji respon fisiologi tanaman padi lokal Sulawesi Utara (varietas Ombong, Temo, TB, dan Sultan) terhadap cekaman banjir pada fase vegetatif berdasarkan kandungan klorofil total pada daun. Pot dan tanaman padi dimasukkan ke dalam ember sehingga terendam setinggi 27 cm di atas permukaan media. Perlakuan cekaman kebanjiran berlangsung selama 20 hari. Pengambilan data dilakukan pada hari ke-0 (sebelum perlakuan), 10, 15 dan 20 setelah perlakuan. Daun diambil sebanyak 1 g kemudian dihaluskan dan diekstraksi menggunakan alkohol 95% dan kandungan klorofil diukur dengan spektrofotometer. Data kandungan klorofil total dalam percobaan faktorial dianalisis dengan analisis sidik ragam dalam rancangan acak lengkap pada tingkat kepercayaan 95%. Hasil penelitian ini menunjukkan bahwa faktor varietas, faktor waktu dan interaksi antara keduanya tidak menunjukkan perbedaan yang nyata pada kandungan klorofil total pada daun tanaman padi (varietas Ombong, Temo, TB, dan Sultan) selama 20 hari perlakuan cekaman banjirThis study examined the physiological responses of North Sulawesi local rice plants in (Ombong, Temo, TB, and Sultan varieties) to partial submergence at the vegetative phase based on the content of total chlorophyll in the rice leaves. The pot and plants were submerged under water in a bucket about 27 cm above the surface of the media. The submergence treatment was lasted for 20 days. Data collection was carried out at day 0 (before treatment), 10, 15 and 20 after treatment. The leaves (about 1 g for ach sample) were collected, then refined and extracted using 95% ethanol. The total chlorophyll content was measured using spectrophotometer. Data of total chlorophyll content in the factorial experiment were analyzed using analysis of variance under completely randomized design at a 95% confidence level. The results of this study indicated that variety and time factors as well as their interactions showed no significant differences in the content of total chlorophyll in the leaves of rice plant varieties (Ombong, Temo, TB, and Sultan) during 20 days of partial submergence

Download Full-text

PENGARUH PENGGANTIAN TEPUNG IKAN DENGAN TEPUNG MAGGOT (Hermetia Illucens) DALAM RANSUM AYAM PEDAGING TERHADAP KECERNAAN KALSIUM DAN FOSFOR

ZOOTEC ◽

10.35792/zot.36.2.2016.11499 ◽

2016 ◽

Vol 36 (2) ◽

pp. 271 ◽

Cited By ~ 1

Author(s):

Heince C. Pesik ◽

J. F. Umboh ◽

C. A. Rahasia ◽

Ch. S. Pontoh

Keyword(s):

Broiler Chicken ◽

Fish Meal ◽

Animal Feed ◽

Dietary Treatment ◽

Hermetia Illucens ◽

Randomized Design ◽

P Content ◽

High Calcium ◽

Key Words Fish ◽

Completely Randomized Design

EFFECT OF SUBSTITUTION OF FISH MEAL WITH MAGGOT (Hermetia Illucens) MEAL ON CALCIUM (Ca) AND PHOSPHORUS (P) DIGESTIBILITY OF BROILER CHICKEN. As an animal feed, maggot from black soldier fly (Hermetia Illucens) has high calcium (Ca) and phosphorus (P) content. Yet, little is known about the digestibility of calcium (Ca) and phosphorus (P) in the diet containing maggot. The present study was designed to determine the effect of substitution of fish meal with maggot (Hermetia illucens) meal in the diets on (Ca) and phosphorus (P) digestibility of broiler chicken. Twenty day old chicks (DOC) CP 707 broiler chicken of about 1300-1500 g body weight were assigned to each dietary treatment which was replicated four times in a Completely Randomized Design (CRD) arrangment. The experimental diet consisted of 5 treatments and 4 replications, formulated as follow: R0 = 100% fish meal + 0% maggot meal; R1 = 75% fish meal + 25% maggot meal; R2 = 50% fish meal + 50% maggot meal; R3 = 25% fish meal + 75% maggot meal; and R4 = 0% fish meal + 100% maggot meal. Calcium (Ca) and phosphorus (P) digestibility were calculated using total collection method. Parameters measured were calcium (Ca) and phosphorus (P) digestibility. Differences were considered significant at P < 0.05. Tukey test (HSD) was employed to further analyze among treatment diferences. The results showed that calcium (Ca) digestibility was significantly (P < 0,05) increased as the level of maggot (Hermetia Illucens) meal in the diets increased; whereas the digestibility of phosphorus (P) was not significantly (P > 0,05) affected by the treatments. It can be concluded utilization of maggot (Hermetia illucens) meal up to 100% replaced fish meal or 15% in the diet, inreased calcium (Ca) digestibility and did not affect phosphorus (P) digestibility of broiler chicken. Key words: Fish meal, Maggot (Hermetia Illucens) meal, Calcium and Phosphours digestibility

Download Full-text

Physical evaluation on freshwater crayfish, Cherax quadricarinatus, feed using several gluten materials

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.2.1.2014.12410 ◽

2014 ◽

Vol 2 (1) ◽

pp. 24

Author(s):

Lady D. Khartiono ◽

Julius Sampekalo ◽

Winda M. Mingkid

Keyword(s):

Control Diet ◽

Quality Analysis ◽

Physical Parameters ◽

Freshwater Crayfish ◽

Cherax Quadricarinatus ◽

Randomized Design ◽

Commercial Feed ◽

Feed Control ◽

Completely Randomized Design ◽

Better Than

Title (Bahasa Indonesia): Uji fisik pakan lobster air tawar, Cherax quadricarinatus, yang menggunakan beberapa bahan perekat The objective of this study was to evaluate the physical parameters of feed for freshwater crayfish (Cherax quadricarinatus) using different glutens. This study used a completely randomized design with four treatments and three replications. Treatment A was feed with starch gluten, treatment B was feed with sago gluten, treatment C was feed with seaweed gluten and treatment D was a commercial feed (control). ANOVA showed that the treatments gave a significant effect (P <0.05) on the breaking rate in the water, sinking speed, homogeneity, and hardness. The results showed that feed using seaweed gluten is very good to use. Feed with starch gluten was also better than feed of sago gluten. However, for sinking speed, control diet and starch gluten-based feed were faster than that of seaweed gluten. The result for water quality analysis including temperature, pH, DO and ammonia during the study showed tolerable ranges for freshwater crayfish. Penelitian ini bertujuan untuk menguji secara fisik pakan lobster air tawar (Cherax quadricarinatus) yang menggunakan beberapa bahan perekat. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan A pakan dengan bahan perekat kanji, perlakuan B pakan dengan bahan perekat sagu, perlakuan C pakan dengan bahan perekat rumput laut dan perlakuan D adalah pakan komersil (kontrol). Hasil analisis ragam menunjukkan bahwa antara perlakuan memberikan pengaruh nyata (P<0,05) terhadap kecepatan pecah pakan dalam air, dispersi pakan, kecepatan tenggelam, homogenitas, dan tingkat kekerasan. Pakan dengan bahan perekat rumput laut sangat baik untuk digunakan. Pakan dengan bahan perekat tepung kanji juga lebih baik dibandingkan pakan berbahan perekat sagu. Tetapi untuk kecepatan tenggelam pakan kontrol dan pakan berbahan perekat kanji lebih cepat dibandingkan pakan berbahan perekat rumput laut. Hasil analisis kualitas air meliputi suhu, pH, DO dan amonia selama penelitian menunjukkan masih dalam batas kisaran yang bisa ditoleransi lobster air tawar.

Download Full-text

Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.1096 ◽

2020 ◽

Vol 12 (1) ◽

pp. 43-49

Author(s):

Verena Agustini ◽

Irma Rahayu ◽

Leonardo A. Numberi ◽

Ziyadatun Ni’mah

Keyword(s):

Positive Influence ◽

Leaf Length ◽

Maximum Effect ◽

Observation Data ◽

Chitosan Concentration ◽

Randomized Design ◽

The Media ◽

Number Of Leaves ◽

Completely Randomized Design

The effect of chitosan on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan had a positive influence on the growth and development of orchids D. lasianthera plantlet in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.

Download Full-text

IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

AGROLAND The Agricultural Sciences Journal ◽

10.22487/j24077593.2017.v4.i2.9540 ◽

2018 ◽

Vol 4 (2) ◽

pp. 60

Author(s):

Asgar Taiyeb ◽

Baharuddin Baharuddi

Keyword(s):

Tissue Culture ◽

Culture Techniques ◽

In Vitro Multiplication ◽

Supportive Environment ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

The Difference ◽

Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

Download Full-text

Pengaruh Penggunaan Sisik Ikan Bandeng Terhadap Kadar Kalsium, Daya Kembang dan Organoleptik Camilan Stick

Jurnal Pangan dan Gizi ◽

10.26714/jpg.9.1.2019.65-73 ◽

2019 ◽

Vol 9 (1) ◽

pp. 65

Author(s):

Chairunnisa Nur Aulia Fajari ◽

Muh Yusuf ◽

Nurrahman Nurrahman

Keyword(s):

Statistical Analysis ◽

Calcium Level ◽

P Value ◽

Fish Scales ◽

Hedonic Value ◽

Randomized Design ◽

Quality Scale ◽

Completely Randomized Design

Stick is snack which favoured by society but lack of nutrition content, especially mineral calcium, then needed an other materials to completed it with the addition of Chanos fish scales fluor. The general purposed of this research was to determined the effect of used Chanos fish scales to calcium levels, swell strength and organoleptic of stick snack. The method of this research is experiments methods used completely randomized design (RAL) monofaktorial by a factor variation of addition 0%, 1%, 2%, 3%, 4% and 5%. Statistical analysis showed an influence from the addition of fish scales fluor to calcium levels and organoleptic that showed from p value <0,05, but no influenced on the swell strength. The best treatment of this research are 3% additional of fish scales with calcium levels 0,38%, swell strength value 1,375%, quality scale value 3,675 and hedonic value 2,83. The conclution of this research are more higher percentage additional of Chanos fish scales will increase calcium level and hedonic value but degraded the quality scale value.

Download Full-text