scholarly journals TOXICITY OF Aaptos suberitoides EXTRACT ON TIGER SHRIMP Penaeous monodon POST LARVAE

2014 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Rosmiati Rosmiati ◽  
Emma Suryati
Keyword(s):  
Normal Growth ◽  
Negative Control ◽  
Marine Sponges ◽  
Abnormal Behavior ◽  
Butanol Extract ◽  
Toxicity Effect ◽  
Tiger Shrimp ◽  
Randomized Design ◽  
Completely Randomized Design

Marine sponges contain bioactive compounds which can be used not only for human diseases treatment but also for animal diseases treatment. Aaptos suberitoides has been reported to have potential as antibacterial against Vibrio harveyi in vitro. This study aims to study the toxicity effect of Aaptos suberitoides extract on tiger shrimp Penaeous monodon post larvae. The toxicity study of this extract was done by using soaking method. This experiment used Completely Randomized Design with 6 treatments of A.suberitoides extract namely A). 0 ppm, B). 31.3 ppm, C). 62.5 ppm, D). 125, E). 250 ppm, and F). 500 ppm. Each treatment consisted of 3 replications. Findings showed that A.suberitoides butanol extracts at the concentration up to125 ppm were safe for tiger shrimp post larvae. On the other hand, the higher concentration of that gave a toxic effect on the post larvae. The lowest mortality (20.0%) was obtained on the post larvae treated with butanol extract at the concentration of 31.3 ppm and the highest was found at the concentration of 500 ppm (97.0%). Compared to the negative control (0 ppm), there was no any difference exhibited by the post larvae treated with butanol extract at the concentrations of 31.3, 62.5, and 125 ppm. Beside that, these concentrations gave a normal growth and no abnormal behavior. The histological observation did not also show damage on post larvae haepatopancreas tissue.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Level ◽  
Piper Nigrum ◽  
Dilution Method ◽  
Randomized Design ◽  
Eryngium Foetidum ◽  
Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

scholarly journals PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

2020 ◽  
Vol 8 (1) ◽  
pp. 18
Author(s):  
Faisal Ansyarif ◽  
Mursal Ghazali ◽  
Aida Muspiah ◽  
Rina Kurnianingsih
Keyword(s):  
Culture Media ◽  
Positive Control ◽  
Negative Control ◽  
Growth Media ◽  
Extract Concentration ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

scholarly journals Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro

2020 ◽  
Vol 151 ◽  
pp. 01046
Author(s):  
Henni Vanda ◽  
Rizki Parindra ◽  
Muhammad Hambal ◽  
Farida Athaillah
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Fasciola Gigantica ◽  
Histopathological Study ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Phosphate Buffered Saline

Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.

scholarly journals Penggunaan Kombinasi Ekstrak Akar Saluang Belum (Lavanga sarmentosa) dengan Akar Kuning (Arcangelisia flava Merr) terhadap Daya Hambat Bakteri Aeromonas hydrophila Secara In Vitro

2020 ◽  
Vol 20 (3) ◽  
pp. 1038
Author(s):  
Maryani Maryani ◽  
Kartika Bungas ◽  
Hasan Anwar
Keyword(s):  
Water Treatment ◽  
Aeromonas Hydrophila ◽  
Negative Control ◽  
Distilled Water ◽  
Clear Zone ◽  
Water Handling ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Inhibitory Power

Saluang Belum Root (Lavanga sarmentosa) and Yellow Root ((Arcangelisia flava) Merr) plants that have antibacterial effects need to be proven, for this research this program uses Saluang Belum Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava) Merr) against bacterial inhibition. Aeromonas hydrophila by In Vitro. The method used in this study was a Completely Randomized Design (CRD) with 6 (six) settings and 3 (three) replications related to the aid of A = 25 gr / 150 ml distilled water, treatment B = 35 gr / 150 ml distilled water, C treatment = 45 gr / 150 ml distilled water, treatment D = 55 gr / 150 ml distilled water, handling E = 500 mg chloromphenicol / 150 ml distilled water, and treatment F = negative control only using the use of 150 ml distilled water. The results of this study indicate a combination of Saluang Belum Root Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava Merr) which is able to inhibit the growth of My Aeromonas hydrophila bacteria because it has an antibacterial effect, this can be seen from the results of the zone average (inhibitory power) on each The treatment is the highest clear zone according to the D 55 gr / 150 ml treatment with an average clear zone of 15 mm and the lowest clear zone yield is A 25 gr / 150 ml approval with an average clear zone value of 11 mm.  Keywords: Aeromonas hydrophila; Root of Saluang Not yet; Yellow Root; Antibacterial.

scholarly journals Pengaruh Retardan Paklobutrazol terhadap Pertumbuhan dan Pemulihan Dua Aksesi Ubi Kayu

2016 ◽  
Vol 11 (3) ◽  
pp. 95
Author(s):  
Surya Diantina ◽  
Darda Efendi ◽  
Ika Mariska
Keyword(s):  
Short Interval ◽  
Normal Growth ◽  
Growth Retardant ◽  
Shoot Length ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Medium Term ◽  
Randomized Design ◽  
Completely Randomized Design

<p>Normal growth medium is not effective for in vitro conservation due to the risk of somaclonal variation that may increase due<br />to short interval between subculture. Two experiments involving growth retardant paclobutrazol (PBZ) were conducted to<br />reduce explants growth and extend subculture interval. In order to develop medium-term conservation of cassava, the<br />recovery of plantlets after in vitro storage was also observed. Accession 433 and 450 were used in two independent<br />experiments. Completely Randomized Design was used with three replications. PBZ at 0, 3.4, 6.8, and 10.2 μM were<br />supplemented onto MS medium + arginin 100 ppm. Observation was done on shoot length, number of nodes and leaves, and<br />number of white and senescence leaves. The results showed that after nine months without subculture, both cassava<br />accessions showed different results in in vitro growth and their recovery. PBZ 3.4 μM performed as the best treatment in<br />accession 433 and 450 to reduce in vitro growth and their recovery after storage.</p>

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

PERBANDINGAN DAYA HAMBAT BAKTERI Pseudomonas fluorescens ASAL TOMAT, KEDELAI, DAN JAGUNG TERHADAP Ralstonia solanacearum SECARA IN VITRO

Agrotek ◽  
2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Aco Roni Kirihio ◽  
Ivonne Fitria Mariay ◽  
Cipta Meliala
Keyword(s):  
Pseudomonas Fluorescens ◽  
Filter Paper ◽  
Ralstonia Solanacearum ◽  
Randomized Design ◽  
Completely Randomized Design

<em>Inhibition of Pseudomonas fluorescens isolates the origin of tomato, soybean and corn against Ralstonia solanacearum tested using a completely randomized design (CRD).        P. fluorescens growth was measured at King's B medium by way of suspension antagonist put on filter paper of 0.5 cm in diameter. Inhibition of P. fluorescens is done by placing the antagonist suspension of 0.5 cm diameter filter paper on NA media that has been deployed R. solanacearum. The results showed that: (a) the growth of P. fluorescens origin of tomato, soybean and corn on King's B media were not significantly different, (b) the inhibition of P. fluorescens isolates against R. solanacearum not significantly different and, (c) the inhibition of isolates P. fluorescens origin of tomato, soybean and corn against R. solanacearum in vitro relatively strong</em>

scholarly journals Seed treatment for management of Meloidogyne javanica in soybean

2017 ◽  
Vol 38 (5) ◽  
pp. 2995 ◽  
Author(s):  
Adriely Alves de Almeida ◽  
Vinicius Hicaro Frederico Abe ◽  
Ricardo Marcelo Gonçalves ◽  
Maria Isabel Balbi-Peña ◽  
Débora Cristina Santiago
Keyword(s):  
Seed Treatment ◽  
Meloidogyne Javanica ◽  
Vitro Assay ◽  
Juvenile Mortality ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Soybean Plants

The objective of this study was to evaluate the efficacy of the active ingredient abamectin, either in isolation or in combination with fungicides and insecticides formulated for the industrial seed treatment, on the population of Meloidogyne javanica, tested under greenhouse and in vitro conditions. In both tests, the combination of the following treatments was assessed: abamectin; thiamethoxam; fludioxonil + metalaxyl-M + thiabendazole. Water was used as control for the in vitro assay, whereas under greenhouse conditions, controls were inoculated and not inoculated with M. javanica. The tests were conducted in a completely randomized design with six (in vitro) and ten (greenhouse) replicates. For in vitro studies the effect on hatching, motility, and mortality of juveniles of M. javanica was evaluated. Under greenhouse conditions, the soybean ‘BRS 133’ seeds were treated, and at 15, 30, and 60 days after inoculation (DAI) with M. javanica, plant measurements were recorded. The penetration of second stage juveniles (J2) was also evaluated at 15 DAI. At 30 DAI, galls, egg masses, nematodes/g of root, and final population were evaluated. At 60 DAI, the final population of nematodes in the roots was quantified. The treatments containing abamectin were the most effective in diminishing the hatching of juveniles. All treatments had an effect on nematode motility when compared to the control, and in the treatment containing only abamectin, total juvenile mortality was observed. In greenhouse conditions, at 15 DAI, the treated soybean plants had the highest root mass and shoot length, differing statistically from the inoculated control. All treatments reduced the number of nematodes per gram of root, differing from the control. At 30 DAI, treatment efficiency was observed in reducing the final population of M. javanica, particularly the treatments using abamectin, and abamectin + thiamethoxam + fludioxonil + metalaxyl-M + thiabendazole. However, at 60 DAI, the effect of the treatments on the population of M. javanica did not persist.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

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