Penggunaan Kombinasi Ekstrak Akar Saluang Belum (Lavanga sarmentosa) dengan Akar Kuning (Arcangelisia flava Merr) terhadap Daya Hambat Bakteri Aeromonas hydrophila Secara In Vitro

Saluang Belum Root (Lavanga sarmentosa) and Yellow Root ((Arcangelisia flava) Merr) plants that have antibacterial effects need to be proven, for this research this program uses Saluang Belum Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava) Merr) against bacterial inhibition. Aeromonas hydrophila by In Vitro. The method used in this study was a Completely Randomized Design (CRD) with 6 (six) settings and 3 (three) replications related to the aid of A = 25 gr / 150 ml distilled water, treatment B = 35 gr / 150 ml distilled water, C treatment = 45 gr / 150 ml distilled water, treatment D = 55 gr / 150 ml distilled water, handling E = 500 mg chloromphenicol / 150 ml distilled water, and treatment F = negative control only using the use of 150 ml distilled water. The results of this study indicate a combination of Saluang Belum Root Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava Merr) which is able to inhibit the growth of My Aeromonas hydrophila bacteria because it has an antibacterial effect, this can be seen from the results of the zone average (inhibitory power) on each The treatment is the highest clear zone according to the D 55 gr / 150 ml treatment with an average clear zone of 15 mm and the lowest clear zone yield is A 25 gr / 150 ml approval with an average clear zone value of 11 mm. Keywords: Aeromonas hydrophila; Root of Saluang Not yet; Yellow Root; Antibacterial.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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POTENSI TUMBUHAN BANGKAL (Nauclea Orientalis) UNTUK PENGENDALIAN BAKTERI Aeromonas Hydrophila (POTENTIAL PLANT BANGKAL (Nauclea orientalis) FOR CONTROL Aeromonas Hydrophila)

Fish Scientiae ◽

10.20527/fs.v2i4.1172 ◽

2016 ◽

Vol 2 (4) ◽

pp. 166

Author(s):

Siti Aisiah

Keyword(s):

Aeromonas Hydrophila ◽

Fish Farming ◽

Quality Parameters ◽

Control Fish ◽

Toxicity Tests ◽

Distilled Water ◽

Normal Range ◽

Water Solvent ◽

Randomized Design ◽

Completely Randomized Design

Tujuan penelitian ini adalah didapatkan metode pengendalian penyakit yang disebabkan oleh bakteri Aeromonas hydrophila pada budi daya ikan yang ramah lingkungan. Dalam penelitian ini dilakukan uji sensitivitas terhadap bakteri A. hydrophila, uji minimal konsentrasi menghambat bakteri A. hydrophila (uji MIC) dan uji toksisitas terhadap ikan nila. Rancangan yang digunakan untuk uji toksisitas adalah rancangan acak lengkap (RAL), dengan 4 perlakuan yaitu A = Ikan disuntik dengan ekstrak bangkal konsentrasi 20%, B = Ikan disuntik dengan ekstrak bangkal konsentrasi 40%, C = Ikan disuntik dengan ekstrak bangkal konsentrasi 80%, dan D = Kontrol (ikan tidak disuntik), diulang sebanyak 3 kali. Perlakuan ini didapat dari hasil uji sensitivitas antibakteri bangkal yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila yaitu ekstrak daun bangkal dengan pelarut akuades. Pengujian MIC menunjukkan bahwa ekstrak bangkal-akuades memiliki daya hambat minimal 20 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan nila dengan konsentrasi 20%, 40% dan 80% mortalitas ikan nila dibawah 50 %. Pengamatan hematologis yaitu eretrosit, leokosit, plasma darah, hematokrit dan leokokrit pada masing-masing perlakuan sebagian besar masih berada dalam kisaran yang normal. Parameter kualitas air yaitu, kadar oksigen terlarut, pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan normal ikan nila.The purpose of this study was obtained method of controlling diseases caused by Aeromonas hydrophila in fish farming is environmentally friendly. In this study the sensitivity of the test A. hydrophila, a minimum test konsentari inhibiting A. hydrophila (MIC test) and toxicity test on tilapia. The design used for toxicity tests is completely randomized design with 4 treatments it A = Fish injected with extracts bangkal concentration of 20 %, B = Fish injected with extracts bangkal concentration of 40 %, C = Fish injected with extracts of 80 % concentration bangkal, and D = Control ( fish not injected ), repeated 3 times. This treatment was obtained from the results of the sensitivity test antibacterial bangkal inhibition and has the power to kill most of the bacteria against A. hydrophila is bangkal leaf extract with distilled water solvent. MIC testing showed that the extract bangkal - distilled water has a minimum of 20 % inhibition of the bacterial activity A. hydrophila. Results of toxicity tests conducted on tilapia with a concentration of 20 %, 40 % and 80 % mortality of tilapia under 50 %. Haematological observation that eretrosit, leokosit, blood plasm, hematocrit and leokokrit in each treatment is still in the normal range. The water quality parameters, dissolved oxygen, pH, ammonia, CO2 and temperature can still support the normal life of tilapia.

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Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

Jurnal Protobiont ◽

10.26418/protobiont.v8i2.34056 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi

Keyword(s):

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Activity Level ◽

Piper Nigrum ◽

Dilution Method ◽

Randomized Design ◽

Eryngium Foetidum ◽

Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

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POTENSI SELADA LAUT Ulva lactuca SEBAGAI ANTIFUNGI DALAM PENGENDALIAN INFEKSI SAPROLEGNIA DAN ACHLYA PADA BUDIDAYA IKAN KERLING (Tor sp)

JURNAL PERIKANAN TROPIS ◽

10.35308/jpt.v5i2.1037 ◽

2018 ◽

Vol 5 (2) ◽

pp. 183

Author(s):

Zulfadhli Zulfadhli ◽

Rinawati Rinawati

Keyword(s):

Survival Rate ◽

Research Method ◽

Ethanol Extract ◽

Ulva Lactuca ◽

Test Results ◽

Clear Zone ◽

Randomized Design ◽

Completely Randomized Design ◽

Fish Survival

This research aims to determine the effectivity of seaweeds extract (Ulva lactuca) as an antifungal in the control of kerling (Tor sp) infected with saprolegnia and achlya fungal. The research was conducted in May 2018 at the MIPA Laboratory, Syiah Kuala University and the Hatchery of the Faculty of Fisheries and Marine Sciences, Teuku Umar University. The research method was experimental with a completely randomized design (RAL), 3 treatments and 3 replications. The treatment given was immersion of kerling fish seeds infected with fungi in a solution of sea lettuce extract with the concentration of treatment namely: K (control) = 0 ppm, P1=5 ppm, P2=10 ppm, dan P3=15 ppm. Phytochemical test results showed that the content of bioactive compounds contained in ethanol extract of seaweed were alkaloids, steroids and phenolic/tannins. Ethanol extract of sea lettuce can inhibit the growth of Saprolegnia and Achlya in vitro by forming a clear zone of 5.7 mm (medium). The highest percentage of fish survival rate was in the treatment (P3) concentration of 15 ppm.

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PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

Bioscientist : Jurnal Ilmiah Biologi ◽

10.33394/bjib.v8i1.2672 ◽

2020 ◽

Vol 8 (1) ◽

pp. 18

Author(s):

Faisal Ansyarif ◽

Mursal Ghazali ◽

Aida Muspiah ◽

Rina Kurnianingsih

Keyword(s):

Culture Media ◽

Positive Control ◽

Negative Control ◽

Growth Media ◽

Extract Concentration ◽

Tissue Culture Media ◽

Randomized Design ◽

Completely Randomized Design ◽

Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

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TOXICITY OF Aaptos suberitoides EXTRACT ON TIGER SHRIMP Penaeous monodon POST LARVAE

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v9i2.98 ◽

2014 ◽

Vol 9 (2) ◽

pp. 75

Author(s):

Rosmiati Rosmiati ◽

Emma Suryati

Keyword(s):

Normal Growth ◽

Negative Control ◽

Marine Sponges ◽

Abnormal Behavior ◽

Butanol Extract ◽

Toxicity Effect ◽

Tiger Shrimp ◽

Randomized Design ◽

Completely Randomized Design

Marine sponges contain bioactive compounds which can be used not only for human diseases treatment but also for animal diseases treatment. Aaptos suberitoides has been reported to have potential as antibacterial against Vibrio harveyi in vitro. This study aims to study the toxicity effect of Aaptos suberitoides extract on tiger shrimp Penaeous monodon post larvae. The toxicity study of this extract was done by using soaking method. This experiment used Completely Randomized Design with 6 treatments of A.suberitoides extract namely A). 0 ppm, B). 31.3 ppm, C). 62.5 ppm, D). 125, E). 250 ppm, and F). 500 ppm. Each treatment consisted of 3 replications. Findings showed that A.suberitoides butanol extracts at the concentration up to125 ppm were safe for tiger shrimp post larvae. On the other hand, the higher concentration of that gave a toxic effect on the post larvae. The lowest mortality (20.0%) was obtained on the post larvae treated with butanol extract at the concentration of 31.3 ppm and the highest was found at the concentration of 500 ppm (97.0%). Compared to the negative control (0 ppm), there was no any difference exhibited by the post larvae treated with butanol extract at the concentrations of 31.3, 62.5, and 125 ppm. Beside that, these concentrations gave a normal growth and no abnormal behavior. The histological observation did not also show damage on post larvae haepatopancreas tissue.

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POTENTIAL EXTRACT of PARE FRUIT (Momordica charantia) AGAINST DIFFERENTIAL LEUKOCYTE IN CARP (Cyprinus carpio) WHICH INFECTED WITH Aeromonas hydrophila

Journal of Fish Health ◽

10.29303/jfh.v1i1.151 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-5

Author(s):

Sri Andayani ◽

Ellana Sanoesi ◽

Oktaviani Setya

Keyword(s):

Bacterial Infection ◽

Aeromonas Hydrophila ◽

Research Method ◽

Negative Control ◽

Blood Collection ◽

Bitter Melon ◽

Fish Meat ◽

Randomized Design ◽

Carp Cyprinus Carpio ◽

Completely Randomized Design

Carp (C. carpio) in intensive production causes fish to be susceptible to disease, one of which is caused by A. hydrophila bacteria. Prevention is often done by giving synthetic chemical antibiotics that can be left in fish meat. Another alternative is to offer natural ingredients, one of which is bitter melon. The aim of the study was to determine the effect of bitter melon extract (M.charantia) on differential leukocytes (lymphocytes, monocytes and neutrophils) in carp (C. carpio) infected with A. hydrophila bacteria. This research method used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used were the administration of different extract doses, namely: treatment A (550 ppm), treatment B (650 ppm), treatment C (750 ppm), and treatment D (850 ppm). positive (K+) with bacterial infection of 107 cells/ml and administration of antibiotics chloramphenicol 0.03 mg/L and negative control (K-) with bacterial infection of 107 cells/ml. Blood collection when the fish are healthy, after offering the bitter gourd extract and after being infected. The results showed that bitter melon extract had a significantly different effect on leukocyte differential. The highest value obtained from treatment D (850 ppm) with the percentage of lymphocytes (18.67-28)%. neutrophils (2.32 = 6.0)% and monocytes (21-26.67)%. In conclusion, treatment with D dose of 850 ppm used as an immunostimulant can increase the differential leukocytes.

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Uji Antagonis Pseudomonas sp. Asal Endofit Perakaran Padi Terhadap Penyakit Blas (Pyricularia oryzae) Secara In Vitro

Agrologia ◽

10.30598/a.v4i2.203 ◽

2018 ◽

Vol 4 (2) ◽

Author(s):

Andree Saylendra ◽

Tubagus Bahtiar Rusbana ◽

Linda Herdiani

Keyword(s):

Pyricularia Oryzae ◽

In Vitro Testing ◽

Antagonistic Bacteria ◽

Pseudomonas Sp ◽

Test Results ◽

Clear Zone ◽

Pseudomonas Spp ◽

Randomized Design ◽

Completely Randomized Design

The purpose of this research was to determine the antagonistic bacteria Pseudomonas spp. of paddy roots endophytic against disease blast (Pycularia oryzae) in vitro. This research was conducted at the Laboratory of Agroecology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa on April to June 2015. This research implemented experimental one factor that arranged in Completely Randomized Design with three replications. The treatment consisted of 9 isolates of Pseudomonas sp. which was screened from endophytic roots of paddy plants. The test results showed that antagonistic in vitro testing could inhibit the growth P. oryzae. Inhibition of 9 isolates to the growth Pseudomonas spp. had the significant clear zone. Isolate Ps 17 (58.89%), Ps 22 (54.44%), Ps 37 (52.06%) and Ps 39 (61.42%) have the largest inhibition. The results showed that the bacteria Pseudomonas sp. on this research is potential on controlling disease blast in paddy plant.

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Pengujian Pelet Berbahan Aktif Trichoderma virens dalam Menekan Pertumbuhan Jamur Akar Putih (JAP) Secara in-vitro

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v2i3.3702 ◽

2020 ◽

Vol 2 (3) ◽

pp. 49-58

Author(s):

Cut Intan Kamila ◽

Tjut Chamzurni ◽

Rina Sriwati

Keyword(s):

Plant Disease ◽

Plant Protection ◽

The Other ◽

Trichoderma Virens ◽

Vitro Fertilization ◽

Randomized Design ◽

Completely Randomized Design ◽

Inhibitory Power ◽

Banda Aceh

Penelitian ini bertujuan untuk mengetahui efektivitas beberapa formulasi pelet berbahan aktif Trichoderma dalam menekan pertumbuhan JAP secara in-vitro. Penelitian ini dilaksanakan pada Agustus sampai Oktober 2016 di Laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman Fakultas Pertanian Universitas Syiah Kuala Banda Aceh. Rancangan Acak Lengkap (RAL) non faktorial, terdiri dari 5 perlakuan dengan 4 ulangan, Setiap perlakuan terdiri dari 3 unit percobaan sehingga diperoleh 60 unit percobaan, adapun parameter yang diteliti adalah daya hambat masing-masing T. virens dengan jamur akar putih. Penelitian ini menggunakan lima formulasi yaitu AT, DAT, KAT, UAT dan JAT. Hasil penelitian menunjukkan persentase daya hambat dari beberapa formulasi pelet yang digunakan formulasi JAT dan KAT pelet T. virens cenderung lebih tinggi pada perlakuan lainnya.Testing Pellets contained Active Trichoderma virens in Suppressing the Growth of White Root Mushroom using in-vitro fertilizationThis study aimed to learn the effectivity of formulation of pellets contained active Trichoderma in surpassing white root mushrooms using in-vitro fertilization. The study was conducted from August to October 2016 in the Laboratory of Plant Disease, Department of Plant Protection, Faculty of Agriculture, Syiah Kuala University. Non-factorial of Completely Randomized Design (CR) consisting of 5 treatments with 4 replications were employed in this study. Each treatment consists of 3 experimental units with the total of 60 experimental units. The examined parameter is the inhibitory power of each T. virens on white root mushrooms. The study used AT, DAT, KAT, UAT, and JAT formulation. The results showed that the percentage of inhibitory power of the JAT and KAT formulation is higher than the other formulations.

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Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro

E3S Web of Conferences ◽

10.1051/e3sconf/202015101046 ◽

2020 ◽

Vol 151 ◽

pp. 01046

Author(s):

Henni Vanda ◽

Rizki Parindra ◽

Muhammad Hambal ◽

Farida Athaillah

Keyword(s):

Methanolic Extract ◽

Anthelmintic Activity ◽

Positive Control ◽

Negative Control ◽

Fasciola Gigantica ◽

Histopathological Study ◽

Randomized Design ◽

Completely Randomized Design ◽

Phosphate Buffered Saline

Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.

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