scholarly journals Study the molecule species of phosphatidylethanolamine class in soft coral \(\textit{Sinularia flexibilis}\) lipid at different times of the year

2021 ◽  
Vol 21 (2) ◽  
pp. 215-222
Author(s):  
Phuong Ly Dang Thi ◽  
Quan Pham Minh ◽  
Nga Nguyen Thi ◽  
Thu Huong Trinh Thi ◽  
Borisovich Andrey Imbs

Samples of soft coral Sinularia flexibilis were collected in February, May, August, November in Nha Trang, Khanh Hoa. The content of molecular species in phosphatidylethanolamine class in the lipid of obtained coral samples has been determined. Identified 14 types of PE molecule species, in which the alkenylacyl PE 18:1e/20:4 has the highest concentration, ranging from 51.35% to 63.16% in 4 samples, the highest and lowest concentrations were in a sample collected in August and November, respectively. Samples collected in August and May have a lower diacyl PE ratio than samples collected in November and February. This study identified the lowest alkylacyl and the highest alkenylacyl concentration in a sample collected in August. The fatty acids present in this class are 17:1, 18:1, 18:2, 19:1, 20:4, and 24:5. The percentage of the total content of molecule species by each fatty acid is similar between months. The total content of molecule species containing 20:4n fatty acids was 80.08–84.27% of the PE class, and the difference was not significant in 4 coral samples. There was an inverse correlation between the total content of molecule species containing 20:4n PUFA and the total content of 20:4n fatty acid and the proportional correlation between the content of PE molecule species containing 24:5n PUFA content of 24:5n fatty acid in total fatty acids.

1980 ◽  
Vol 43 (2) ◽  
pp. 367-373 ◽  
Author(s):  
W. M. Tsagn ◽  
J. Belin ◽  
A. D. Smith

1. When weanling rats were fed on a diet containing 0.1 g/kg of the diet as polyunsaturated fatty acid, it was found that after 2 weeks the level of linoleate in the lymphocyte total lipids was 56 mg/ g total fatty acids, as compared with a level of 138 mg/ g in rats on a normal diet (P < 0.005). Similar levels were obtained from rats which had been fed for up to 16 weeks on the deficient diet, but in a group killed after 28 weeks on the diet the level was found to be only 20 mg/ g total fatty acids. The arachidonate level was found to be approximately 220 mg/ g total fatty acids, regardless of whether the rats were fed on a diet deficient in linoleate for up to 16 weeks or on a normal diet. In the group of rats killed after 28 weeks on the linoleate deficient diet, however, the arachidonate level was only 60 mg/ g total fatty acids.2. Percentage values for total fatty acids are given for plasma, adipose tissue, and lymphocytes for rats on normal and experimental diets.3. Scatter diagrams of the levels of linoleate v. arachidonate in the lymphocyte total fatty acids showed no correlation between the levels of the two acids (r 0.05), but similar plots of linoleate and oleate levels showed an inverse correlation (r – 0.68).


Author(s):  
A. A. Hamid ◽  
W. M. W. Yusof ◽  
R. M. Illias ◽  
K. Nadarajah

Kajian telah dijalankan untuk mendapatkan strain baru kulat yang menghasilkan GLA hasil penyaringan 21 pencilan yang diperoleh daripada tanah hutan simpan di sekitar Selangor dan Negeri Sembilan. Sebanyak 5 pencilan kulat daripada 21 pencilan yang diperoleh telah dikenalpasti menghasilkan asid γ–linolenik (GLA) (18:3, n-6) iaitu SK8, SK22, 2A1, DR dan K3, yang menghasilkan 6%, 7.6%, 6.9% 5.4% dan 13.5% GLA daripada asid lemak total masing-masing. Kelima-lima pencilan tersebut juga didapati menghasilkan peratusan lipid yang berbeza, iaitu 30%, 20%, 32%, 30% dan 8% lipid total (w/w, biojisim), masing-masing. Pencilan K3 mempunyai kandungan GLA yang setara dengan kandungan GLA dalam minyak ‘Evening Primrose’ yang dihasilkan secara komersial pada masa kini, iaitu 8-12% GLA dari asid lemak total. Pencilan 2A1 menunjukkan penghasilan GLA tertinggi (0.0096g GLA/g glukosa) berbanding SKA, SK22, DR dan K3, yang menghasilkan 0.0032. 0.0039, 0.007 dan 0.0025g, GLA/g glukosa, masing-masing. Perbezaan profil asid lemak bagi keseluruhan 21 pencilan juga dibincangkan. Kata kunci: Oleaginous; kulat; γ-linolenic; asid lemak poli-tidak-tepu. In our studies, 21 filamentous fungi were isolated from soil obtained from various sites of forest reserves in Selangor and Negeri Sembilan. Five isolates (SK8, SK22, 2A1, DR and K3) were found to produced γ–linolenic acid (GLA) (18:3, n–6) at 6%, 7.6%, 6.9%, 5.4% and 13.5% of their respective total fatty acid with lipid contents of 30%, 20%, 32%, 30% and 8% (w/w, biomass), respectively. The GLA content of K3 is comparable to that of the commercially available ‘Evening Primrose’ with GLA comprising of 8–12% of its total fatty acids. The difference in the fatty acid profiles from all 21 isolates is discussed here. Key words: Oleaginous; fungi γ-linolenic; polyunsaturated fatty acids.


2000 ◽  
Vol 57 (S3) ◽  
pp. 31-37 ◽  
Author(s):  
G C Cripps ◽  
A Atkinson

In a previous study at South Georgia, carnivory was invoked as a cause of high polyunsaturated fatty acid (PUFA) content of Antarctic krill, Euphausia superba. To examine this, krill were sampled and fed for 16 days exclusively on the locally abundant copepod Drepanopus forcipatus. After 16 days, the krill had increased their PUFA content from 28 to 54% of the total fatty acids. Concurrently, monounsaturated fatty acids (MUFAs) and saturated fatty acids (SFAs) decreased from 41 to 27%. Thus, the krill appeared to accumulate PUFAs as reflected in their diet of D. forcipatus, which also had a relatively high PUFA content (50%). Overall, the results support omnivorous feeding by krill at South Georgia during nonbloom periods. We propose that the ratio of PUFA to SFA content may be used to detect carnivory in the recent feeding history of krill and suggest that this may be an index which could be applied to other zooplankton.


2021 ◽  
Vol 11 (5) ◽  
pp. 2409
Author(s):  
Wojciech Kolanowski

Salmonids are valuable fish in the human diet due to their high content of bioactive omega-3 very long-chain polyunsaturated fatty acid (VLC PUFA). The aim of this study was to assess the omega-3 VLC PUFA content in selected salmonid fish present on the food market regarding whether they were farm-raised or wild. It was assumed that farm-raised fish, by eating well-balanced feed enriched with omega-3 PUFA, might contain omega-3 VLC PUFA in levels similar to that of wild fish. Fat content, fatty acid composition and omega-3 VLC PUFA content in fish fillets were measured. Farm-raised salmon from Norway, wild Baltic salmon, farm-raised rainbow trout and brown trout were bought from a food market whereas wild trout (rainbow and brown) were caught alive. The fat content in fish ranged from 3.3 to 8.0 g/100 g of fillet. It was confirmed that although wild salmonid fish contain 10–25% more omega-3 VLC PUFA in lipid fraction, the farm-raised ones, due to the 60–100% higher fat content, are an equally rich source of these desirable fatty acids in the human diet. One serving (130 g) of salmonid fish fillets might provide a significant dose of omega-3 VLC PUFA, from 1.2 to 2.5 g. Thus, due to very high content of bioactive fatty acids eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) in their meat, salmonid fish currently present on the food market, both sea and freshwater as well as wild and farm-raised, should be considered as natural functional food.


Author(s):  
Ikumi Umetani ◽  
Eshetu Janka ◽  
Michal Sposób ◽  
Chris J. Hulatt ◽  
Synne Kleiven ◽  
...  

AbstractBicarbonate was evaluated as an alternative carbon source for a green microalga, Tetradesmus wisconsinensis, isolated from Lake Norsjø in Norway. Photosynthesis, growth, and lipid production were studied using four inorganic carbon regimes: (1) aeration only, (2) 20 mM NaHCO3, (3) 5% (v/v) CO2 gas, and (4) combination of 20 mM NaHCO3 and 5% CO2. Variable chlorophyll a fluorescence analysis revealed that the bicarbonate treatment supported effective photosynthesis, while the CO2 treatment led to inefficient photosynthetic activity with a PSII maximum quantum yield as low as 0.31. Conversely, bicarbonate and CO2 treatments gave similar biomass and fatty acid production. The maximum growth rate, the final cell dry weight, and total fatty acids under the bicarbonate-only treatment were 0.33 (± 0.06) day−1, 673 (± 124) mg L−1 and 75 (± 5) mg g−1 dry biomass, respectively. The most abundant fatty acid components were α-linolenic acid and polyunsaturated fatty acids constituting 69% of the total fatty acids. The fatty acid profile eventuated in unsuitable biodiesel fuel properties such as high degree of unsaturation and low cetane number; however, it would be relevant for food and feed applications. We concluded that bicarbonate could give healthy growth and comparative product yields as CO2.


2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Christel Brunschwig ◽  
François Xavier Collard ◽  
Jean-Pierre Bianchini ◽  
Phila Raharivelomanana

In order to establish a chemical fingerprint of vanilla diversity, thirty samples of V. planifolia J. W. Moore and V. tahitensis G. Jackson cured beans from seven producing countries were examined for their aroma and fatty acid contents. Both fatty acid and aroma compositions were found to vary between vanilla species and origins. Vanillin was found in higher amounts in V. planifolia (1.7-3.6% of dry matter) than in V. tahitensis (1.0-2.0%), and anisyl compounds were found in lower amounts in V. planifolia (0.05%) than in V. tahitensis (1.4%-2.1%). Ten common and long chain monounsaturated fatty acids (LCFA) were identified and were found to be characteristic of the vanilla origin. LCFA derived from secondary metabolites have discriminating compositions as they reach 5.9% and 15.8% of total fatty acids, respectively in V. tahitensis and V. planifolia. This study highlights the role of the curing method as vanilla cured beans of two different species cultivated in the same country were found to have quite similar fatty acid compositions.


1998 ◽  
Vol 6 (1) ◽  
pp. 229-234 ◽  
Author(s):  
William R. Windham ◽  
W.H. Morrison

Near infrared (NIR) spectroscopy in the prediction of individual and total fatty acids of bovine M. Longissimus dorsi neck muscles has been studied. Beef neck lean was collected from meat processing establishments using advanced meat recovery systems and hand-deboning. Samples ( n = 302) were analysed to determine fatty acid (FA) composition and scanned from 400 to 2498 nm. Total saturated and unsaturated FA values ranged from 43.2 to 62.0% and 38.3 to 56.2%, respectively. Results of partial least squares (PLS) modeling shown reasonably accurate models were attained for total saturate content [standard error of performance ( SEP = 1.10%); coefficient of determination on the validation set ( r2 = 0.77)], palmitic ( SEP = 0.94%; r2 = 0.69), unsaturate ( SEP = 1.13%; r2 = 0.77), and oleic ( SEP = 0.97; r2 = 0.78). Prediction of other individual saturated and unsaturated FAs was less accurate with an r2 range of 0.10 to 0.53. However, the sum of individual predicted saturated and unsaturated FA was acceptable compared with the reference method ( SEP = 1.10 and 1.12%, respectively). This study shows that NIR can be used to predict accurately total fatty acids in M. Longissimus dorsi muscle.


1973 ◽  
Vol 30 (2) ◽  
pp. 181-185 ◽  
Author(s):  
John W. Farrington ◽  
James G. Quinn ◽  
Wayne R. Davis

Samples of the infaunal invertebrates Nephtys incisa and Yoldia limatula from Narragansett Bay, Rhode Island, have been analyzed for their fatty acid distribution. Based on total fatty acids, Yoldia contains 9–16% of an acid tentatively identified as 22:2. The ratios of 18:1/18:0 and 18:1/20:1 fatty acids of Nephtys from a polluted station in the bay are lower than the corresponding ratios for animals from relatively clean areas.


PEDIATRICS ◽  
1987 ◽  
Vol 80 (4) ◽  
pp. 517-523
Author(s):  
Dominique C. Belli ◽  
Emile Levy ◽  
Pauline Darling ◽  
Claudie Leroy ◽  
Guy Lepage ◽  
...  

The effect of taurine supplementation on the absorption of a fat meal was evaluated in patients with cystic fibrosis. In a cross-over design study, five patients with cystic fibrosis (12.1 ± 2.6 years of age) and three control subjects received either placebo or taurine (30 mg/kg/d) for two 1-week periods, a month apart, followed by a fat meal test. Blood samples were drawn 0, 1, 2, 3, 5, 8 hours after the meal. Four patients with cystic fibrosis and severe steatorrhea despite appropriate enzyme therapy showed a significant (P &lt; .05) improvement in the absorption of triglycerides, total fatty acids, and linoleic acid while receiving taurine supplements. Three control subjects and one child with cystic fibrosis and mild steatorrhea receiving enzyme therapy did not experience such an effect. The difference in triglyceride absorption, when calculated as the area under the curve, receiving and not receiving taurine was significantly (P &lt; .05) correlated with the degree of steatorrhea. Furthermore, in contrast to control subjects, the fatty acid composition of chylomicrons in these four study patients showed important discrepancies with that of the fat meal and was corrected, in part, by taurine supplementation. These results suggest that taurine supplementation could be a useful adjunct in the management of patients with cystic fibrosis with ongoing fat malabsorption and essential fatty acid deficiency.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Yutaka Tashiro

Purpose This study aimed to analyze the lipid content and fatty acid composition in the liver and muscle of a porcupinefish species inhabiting waters around the Ryukyu Islands to investigate their potential as a source of long-chain n-3 polyunsaturated fatty acids (LC-PUFAs). Design/methodology/approach Porcupinefish were collected along the Okinawa Island coast. The composition of fatty acids and cholesterol in both liver and muscle were analyzed using a gas chromatograph mass spectrometer. Findings The liver of Okinawan long-spine porcupinefish was rich in lipids whose content correlated to the proportion of liver/body weight. Fatty acid compositions in their liver and muscles were similar to each other. LC-PUFAs occupied 44% of total fatty acids, with docosahexaenoic acid (DHA) being the dominant (42%), whereas eicosapentaenoic acid occupied 2.4%. The liver contained 1,690 mg of cholesterol and 14.8 g of DHA per 100 g, whose proportion decreased in summer compared to other seasons (p = 0.036). Originality/value The liver of Okinawan long-spine porcupinefish, which has not yet been commercially used although its non-toxicity is claimed, can be an excellent source of LC-PUFAs, especially DHA, accentuating its potential in food supplements’ production.


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