scholarly journals INVESTIGATION OF ENZYMATIC HYDROLYSIS OF FATTY WASTE PROCESSING OF SUNFLOWER SEEDS BY IMMOBILIZED LIPASE

2020 ◽  
Vol 20 (1) ◽  
pp. 5-11
Author(s):  
V. Skliar ◽  
G. Krusir ◽  
V. Zakharchuk
Keyword(s):  
Crop Production ◽  
Agricultural Sector ◽  
Lipolytic Activity ◽  
Immobilized Lipase ◽  
Immobilized Enzymes ◽  
Lipase Immobilization ◽  
Ph Optimum ◽  
Prolonged Incubation ◽  
Physical And Chemical ◽  
Hydrolysis Of

Ukraine has a developed agricultural sector, in particular crop production, which is a source of large quantities of production residues and waste. One of the most promising areas for solving environmental problems in the production of grain products is the processing of industrial waste by enzymes and the use of processing products in other industries. The current needs of sustainable environmental practices have increased the use of enzymatic technologies in production processes. Lipases be used in the processing of waste from oil and fat enterprises, namely, waste from the stage of demetallization of hydrogenated fat from vegetable oils by enzymatic hydrolysis.The work is devoted to the study of conditions of enzyme Rhizopus japonicus lipase immobilization and its physical and chemical characteristics. Factors for obtaining immobilized biocatalysts, methods and conditions for determining the activity and stability of immobilized enzymes are highlighted. Lipolytic activity of the enzyme immobilized under these conditions remains more than 30% compared to native, which is a high indicator of activity retention. It has been shown that immobilization promotes the expansion of the pH- and thermo-optimum of the lipase. It was determined that for the Rhizopus japonicus immobilized lipase, the pH optimum increased with a shift from 7.0 to 6.5, and there was an increase in pH stability during prolonged incubation of the immobilized enzyme for alkaline and acidic pH values. It has been established that lipase immobilization leads to expansion of the thermo-optimum, as well as stabilization of the enzyme during prolonged incubation at 40 ° C and at higher temperatures (+60-80 ° C), which are possible when drying the final product. The high activity and stability of the immobilized lipase make it possible to recommend it for biotechnological processing ofoil-fat waste.

scholarly journals Study of the physical and chemical characteristics of an immobilized lipase in the hydrolysis of fat waste

2020 ◽  
Vol 31 (3) ◽  
pp. 25
Author(s):  
Viktoriia Skliar ◽  
Galina Krusir ◽  
Kuznecova Iryne ◽  
Valentyna Zakharchuk ◽  
Myroslav Malovanyy
Keyword(s):  
Immobilized Lipase ◽  
Chemical Characteristics ◽  
Physical And Chemical Characteristics ◽  
Physical And Chemical ◽  
Hydrolysis Of

scholarly journals Pereparation and characterization of two types of covalently immobilized amyloglucosidase

2005 ◽  
Vol 70 (5) ◽  
pp. 713-719 ◽  
Author(s):  
Nenad Milosavic ◽  
Radivoje Prodanovic ◽  
Slobodan Jovanovic ◽  
Irena Novakovic ◽  
Zoran Vujcic
Keyword(s):  
Specific Activity ◽  
Periodate Oxidation ◽  
Immobilized Enzymes ◽  
Soluble Starch ◽  
Soluble Enzyme ◽  
Temperature Optimum ◽  
Ph Optimum ◽  
Higher Temperature ◽  
Hydrolysis Of

Amyloglucosidase from A. niger was covalently immobilized onto poly( GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined. .

scholarly journals Toward controlled geometric structure and surface property heterogeneities of TiO2 for lipase immobilization

2020 ◽  
Author(s):  
Wenfeng Zhou ◽  
Xiaohong Zhou ◽  
Wei Zhuang ◽  
Rijia Lin ◽  
Ye Zhao ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Pore Size ◽  
Surface Properties ◽  
Geometric Structure ◽  
Immobilized Enzyme ◽  
Immobilized Lipase ◽  
Immobilized Enzymes ◽  
Relative Activity ◽  
Lipase Immobilization ◽  
Enzyme Molecules

Immobilized enzymes as biocatalysts are expected to solve issues of pollution and economic inefficiency in industrial catalysis. In order to obtain an immobilized enzyme with high activity and stability, the design of substrate geometric structure and surface properties is desirable. Here, TiO2 with controlled pore size and surface properties was designed and synthesized for lipase immobilization, resulting in an efficient biocatalyst. The activity of TiO2 immobilized lipase is improved with the increasing pore size of TiO2 from 10 to 100 nm. Compared to geometric structure impact, regulation of surface properties plays a greater role on the immobilization of lipase on TiO2. Among them, the relative activity of ethenyl triethoxy silane (ETS) modified TiO2 immobilized lipase is as high as 365.85 % over the pristine lipase. This research provides experimental evidence for studying the adsorption of enzyme molecules on the supports under the synergistic effect of geometric structure and surface properties.

A Macroeconomic Analysis of Agricultural Sector in Nigeria

2019 ◽  
Vol 5 (1) ◽  
pp. 18-25
Author(s):  
Isah Funtua Abubakar ◽  
Umar Bambale Ibrahim
Keyword(s):  
Value Chain ◽  
Crop Production ◽  
Agricultural Sector ◽  
Time Series Data ◽  
Tourism Industry ◽  
Series Data ◽  
Size Analysis ◽  
The Real ◽  
Real Gdp ◽  
The Impact

This paper attempts to study the Nigerian agriculture industry as a panacea to growth as well as an anchor to the diversification agenda of the present government. To do this, the time series data of the four agriculture subsectors of crop production, livestock, forestry and fishery were analysed as stimulus to the Real GDP from 1981-2016 in order to explicate the individual contributions of the subsectors to the RGDP in order to guide the policy thrust on diversification. Using the Johansen approach to cointegration, all the variables were found to be cointegrated. With the exception of the forestry subsector, all the three subsectors were seen to have impacted on the real GDP at varying degrees during the time under review. The crop production subsector has the highest impact, however, taking size-by-size analysis, the livestock subsector could be of much importance due to its ability to retain its value chain and high investment returns particularly in poultry. Therefore, it is recommended that, the government should intensify efforts to retain the value chain in the crop production subsector, in order to harness its potentials optimally through the encouragement of the establishment of agriculture cottage industries. Secondly, the livestock subsector is found to be the most rapidly growing and commercialized subsector. Therefore, it should be the prime subsector to hinge the diversification agenda naturally. Lastly, the tourism industry which is a source through which the impact of the subsector is channeled to the GDP should be developed, in order to improve the impact of such channel to GDP with the sole objective to resuscitate the forestry subsector.

Polarographic study of hydrolysis of [8-lysine]vasopressin and its derivatives with blood serum of pregnant women

1980 ◽  
Vol 45 (4) ◽  
pp. 1099-1108 ◽  
Author(s):  
Mikuláš Chavko ◽  
Michal Bartík ◽  
Evžen Kasafírek
Keyword(s):  
Blood Serum ◽  
Pregnant Women ◽  
Degree Of Hydrolysis ◽  
Polarographic Study ◽  
Ph Optimum ◽  
Lysine Vasopressin ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Vasopressin Analogues

A polarographic study of the hydrolysis of [8-lysine]vasopressin and some hormonogens of the vasopressin series with the blood serum of women in the last week of pregnancy was studied. The dependence of hydrolysis on pH (pH optimum: 7.4-7.50, substrate concentration (Km 1.2 . 10-5M), pH stability and thermal stability were determined. The rate of hydrolysis of individual vasopressin analogues decreases in the order: [8-lysine]vasopressin > Nα-glycyl-prolyl[8-lysine]-vasopressin > Nα-leucyl-[8-lysine]vasopressin > Nα-alanyl-[8-lysine]vasopressin > Nα-phenyl alanyl-[8-lysine]vasopressin > Nα-diglycyl-[8-lysine]vasopressin > Nα-prolyl-[8-lysine]vasopressin > Nα-triglycyl-[8-lysine]vasopressin > Nα-sarcosyl-glycyl-[8-lysine]vasopressin. The degree of hydrolysis gradually increases to a multiple with the length of the pregnancy in consequence of the presence of oxytocine. However, vasopressin is also hydrolysed to a small extent with the enzymes from the blood sera of non-pregnant women. Under similar analytical conditions oxytocin was not hydrolysed with the sera of non-pregnant women and therefore oxytocin is a more suitable substrate than vasopressin for polarographic determination of serum oxytocinase.

Aspects of lipid synthesis, hydrolysis, and transport studied in selected Amazon fish

1978 ◽  
Vol 56 (4) ◽  
pp. 787-792 ◽  
Author(s):  
John S. Patton ◽  
Monty S. Haswell ◽  
Thomas W. Moon
Keyword(s):  
Fatty Acid ◽  
Lipolytic Activity ◽  
Lipid Synthesis ◽  
Blood Lipid ◽  
Dietary Lipid ◽  
Indwelling Catheter ◽  
Ph Optimum ◽  
Mesenteric Fat ◽  
Fatty Acid Release ◽  
Amazon Fish

Comparative lipogenic activities offish tissue, lipolytic activity of mesenteric fat and mode of intestinal lipid release into blood were investigated in a variety of Amazon fish. Small catfish were injected intramuscularly with [1-l4C]acetate, killed at intervals, and the lipid radioactivity of 11 separate tissues determined. In 6- and 18-h fish, the heart, eyes, dark muscle, and white muscle synthesized negligible lipid relative to the other tissues. Acetone powders of Triportheus sp, mesenteric fat contained high amounts of triglyceride lipase activity (120 nmol fatty acid release-d/min per milligram protein). The activity exhibited a pH optimum of 8.0 and was not activated by albumin, bile salts, or divalent salts nor inhibited by 1 M NaCl. Characteristics of the observed activity are identical with those of mammalian pancreatic lipase. Hoplias malabaricus were fed [1-14C]oleic acid and a chronic indwelling catheter was placed in the dorsal aorta for blood sampling. Based on the distribution of radioactivity among blood lipid classes, it is suggested that dietary lipid enters fish circulatory systems both as free fatty acid and as lipoproteins.

Перспективи розвитку зерновиробництва в Кіровоградській області

Ekonomika APK ◽  
2021 ◽  
Vol 319 (5) ◽  
pp. 30-37
Author(s):  
Emma Symonian ◽  
Nataliia Nerubaiska ◽  
Maryna Pukhliakova ◽  
Maksym Yandola
Keyword(s):  
Crop Production ◽  
Systematic Approach ◽  
Agricultural Sector ◽  
Security Analysis ◽  
Practical Significance ◽  
Grain Production ◽  
Production Development ◽  
Analysis And Synthesis ◽  
National Production ◽  
Yield Monitoring

The purpose of the article is to explore the prospects for the development of grain production in the Kirovograd region. Research methods. The study used dialectical methods of cognition, a systematic approach to the study of economic phenomena, monographic (generalizing domestic and foreign experience in determining the economic essence of food security), analysis and synthesis (in studying the levels of production of cereals and legumes and the level of grain supply), statistical groups (in identifying the place of regions in the national production of cereals), economic and statistical (in the processing of mass statistics), abstract and logical (in substantiating theoretical generalizations and drawing conclusions). Research results. The potential of grain production development in Kirovohrad region is considered. The analysis is based on statistical data of the crop industry. Problems of industry development are revealed and ways of solutions are offered. Scientific novelty. Analysis of the level of crop production in the Kirovohrad region in terms of using the latest technologies and yield monitoring. Practical significance. Along with some positive changes in the region's grain production, obstacles to their development have been identified. Proposals have been made to eliminate them with active investments and state support programs for the agricultural sector of the region. Tabl.: 4. Figs.: 1. Refs.: 18.

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

2021 ◽  
pp. 22-41
Keyword(s):  
Organophosphorus Compounds ◽  
Degradation Products ◽  
Rapid Development ◽  
Hydrolytic Enzymes ◽  
Immobilized Enzymes ◽  
Amino Acid Sequences ◽  
Practical Implementation ◽  
Scientific Results ◽  
Physical And Chemical

Еnzymes are able to effectively interact with various organophosphorus compounds (OPC), entering into (bio)chemical reactions with them. Changes in the initial activity of enzymes as a result of their inhibition by OPC, the formation of OPC degradation products under the action of hydrolytic enzymes, etc. can be determined using different physical and chemical methods and used in bioanalytic systems to determine the concentrations of OPC. The purpose of the review is to analyze the main scientific results achieved over the past 10 years in the development of analytical systems based on enzymes intended for the determination of OPC. It is shown in the article, that the requirements for the sensitivity of biosensors are based on the norms of the content of the analyzed substances detected in/at the objects of mandatory control. The cholinesterases compose a basis for the development of the largest number of ultra-sensitive biosensors, although other enzymes can be successfully used as a biosensitive element. The most technologically advanced solution that is close to the practical implementation seems to be bioanalytical systems using immobilized enzymes. Improving the detection limits of the OPC can be achieved by using nanoobjects together with modern methods of signal transducers, for example, with nanomechanical detectors and signal converters. This combination of technical solutions ensures the sensitivity of the OPC analysis up to pg/l. At present, «reagentless» systems have received significant development, which have become the basis for the production of a large number of commercially available strips for the express determination of OPC. Modern demands stimulate the rapid development of portable and, especially, wearable biosensors that can be attached to various surfaces, including a clothing. The progress in the development of affine amino acid sequences, in the future, will allow the creation of enzyme biosensors on any surface.

scholarly journals Growing climatic sensitivity of U.S. agriculture linked to technological change and regional specialization

2018 ◽  
Vol 4 (12) ◽  
pp. eaat4343 ◽  
Author(s):  
Ariel Ortiz-Bobea ◽  
Erwin Knippenberg ◽  
Robert G. Chambers
Keyword(s):  
Climate Change ◽  
Crop Production ◽  
Agricultural Sector ◽  
State Level ◽  
The United States ◽  
Climate Data ◽  
Specialty Crops ◽  
Regional Specialization ◽  
Vulnerability To Climate Change ◽  
Climatic Sensitivity

A pressing question for climate change adaptation is whether ongoing transformations of the agricultural sector affect its ability to cope with climatic variations. We examine this question in the United States, where major increases in productivity have fueled most of agricultural production growth over the past half-century. To quantify the evolving climate sensitivity of the sector and identify its sources, we combine state-level measures of agricultural productivity with detailed climate data for 1960–2004. We find that agriculture is growing more sensitive to climate in Midwestern states for two distinct but compounding reasons: a rising climatic sensitivity of nonirrigated cereal and oilseed crops and a growing specialization in crop production. In contrast, other regions specialize in less climate-sensitive production such as irrigated specialty crops or livestock. Results suggest that reducing vulnerability to climate change should consider the role of policies in inducing regional specialization.

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