Effects of intranasal Imunofan administration upon phagocytic activity in treatment of exudative otitis media in children

Exudative otitis media in childhood is most often associated with chronic inflammation in the nasopharyngeal area, with immediate participation of phagocytic cells. Our paper presents the data on evaluation of clinical and immunological efficacy of intranasal Imunofan use included into complex therapy of exudative otitis media. Dynamic observation (before treatment, 1 and 3 months after treatment) of these parameters included regular evaluation of the neutrophil and monocyte amounts in peripheral blood and in smear imprints from nasal mucosa, determination of myeloperoxidase activity in circulating neutrophils, and the content of interleukin IL-8 and IL-18 in the nasal washouts. The clinical status was assessed using a scoring system, which subjectively reflected the state of the nasopharynx and auditory function. Fourty-three children aged from 3 to 7 years with exudative otitis media associated with chronic adenoiditis were examined. Patients of the first group (22 children) were treated using only conventional approaches (basic therapy). The patients from the second group (21 children) received Imunofan in addition to the basic therapy. The control group consisted of 16 relatively healthy children. Before treatment of the children with exudative otitis media, an increase in the relative content of monocytes in their blood, a decreased activity of myeloperoxidase and lower concentration of IL-8 and IL-18 in the nasal wash was observed in comparison with healthy controls. No differences in severity of clinical symptoms were revealed between the groups of patients. Baseline therapy was not accompanied by positive dynamics in the clinical pattern of the disease. Relative monocytosis and reduced activity of neutrophilic myeloperoxidase persisted in peripheral blood; the concentration of IL-8 and IL-18 in the nasal washings remained low. Following intranasal use of Imunofan, the number of circulating monocytes was restored by the third month from the start of treatment, there was an increased activity of myeloperoxidase registered in blood neutrophils, as well as higher IL-8 and IL-18 concentrations in the nasal washings. Normalization of the phagocytos-related parameters, according to this scoring, was associated with clinical remission of the disease. The revealed relationships between clinical data and the results obtained in the course of laboratory research suggest a positive effect of Imunofan as an agent that may enhance effectiveness of conventional basic therapy of otitis media in children.

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Relationship of T lymphocytes, cytokines, immunoglobulin E and nitric oxide with otitis media with effusion in children and their clinical significances

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.65.7.971 ◽

2019 ◽

Vol 65 (7) ◽

pp. 971-976

Author(s):

Wenyan Fan ◽

Xiaoyan Li ◽

Hongming Xu ◽

Limin Zhao ◽

Jiali Zhao ◽

...

Keyword(s):

Nitric Oxide ◽

T Lymphocytes ◽

Otitis Media ◽

Peripheral Blood ◽

Immunoglobulin E ◽

Otitis Media With Effusion ◽

Control Group ◽

Healthy Children ◽

Observation Group ◽

Cd8 T Lymphocyte

SUMMARY OBJECTIVE To investigate the relations of T lymphocytes, cytokines, immunoglobulin E, and nitric oxide with otitis media with effusion (OME) in children and their clinical significances. METHODS Fifty children with OME treated in our hospital were enrolled in the study (observation group). Fifty healthy children were selected as control. The percentages of CD4+ and CD8+ T lymphocyte and CD4+/CD8+ ratio in peripheral blood, and the levels of cytokine (IL)-2, IL-4, IL-6, immunoglobulin E (IgE) and nitric oxide (NO) in peripheral blood and middle ear effusion (MEE) in both groups were detected. The correlations of these indexes with OME were analyzed. RESULTS The percentage of peripheral blood CD4+ and CD8+ levels, CD4+/CD8 ratio, IgE, and NO levels in the observation group were significantly higher than those in the control group (P < 0.01). In the observation group, the IL-2 and IL-6 levels, and IgE and NO levels in the MEE were significantly higher than those in peripheral blood (P < 0.01). In addition, in the observation group, the MEE IL-2 and IL-6 levels were positively correlated with peripheral blood CD4+/CD8+ ratio, respectively r = 0.366, P = 0.009; r = 0.334, P = 0.018. CONCLUSIONS The levels of peripheral blood CD4+ and CD8+ lymphocytes and MEE IL-2, IL-6, IgE, and NO levels are increased in children with OME. These indexes have provided significant clues for the diagnosis of OME in children.

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Comparative expression analysis of human endogenous retrovirus elements in peripheral blood of children with specific language impairment

Balkan Journal of Medical Genetics ◽

10.2478/bjmg-2019-0014 ◽

2019 ◽

Vol 22 (1) ◽

pp. 49-56

Author(s):

DS Minchev ◽

NT Popov ◽

SI Naimov ◽

IN Minkov ◽

TI Vachev

Keyword(s):

Dna Sequences ◽

Language Impairment ◽

Specific Language Impairment ◽

Peripheral Blood ◽

Expression Profiles ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Control Group ◽

Healthy Children ◽

Specific Language

AbstractSpecific language impairment (SLI) is a psychiatric condition with a complex etiology and a substantial genetic basis that affects children's verbal communication abilities. In this study, we examined the expression of five different human endogenous retrovirus elements (HERVs) in a cohort of 25 children with SLI and 25 healthy children in the control group. Human endogenous retrovirus elements, a diverse group of repetitive DNA sequences, can potentially cause considerable genetic heterogeneity. They had been integrated in the genome of our ancestors throughout evolution and now consist of about 8.0% of the human genome. Several HERV loci are transcribed in various cell types. Their expression in peripheral blood and in the brain is altered in many neurological and psychiatric diseases. To date, HERV expression profiles have never been studied in patients with SLI. This study aimed to elucidate differentially regulated human endogenous retroelements in peripheral blood of children with SLI, in comparison with healthy controls, through quantitative reverse tran-scription-polymerase chain reaction (qRT-PCR) methodology. Our results show that two genes: HERV-K (HLM-2) gag and HERV-P env were expressed at lower levels in the blood samples from SLI children in comparison with those in the control group.

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Diagnostic Value of the TpTe Interval in Children with Ventricular Arrhythmias

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph182212194 ◽

2021 ◽

Vol 18 (22) ◽

pp. 12194

Author(s):

Joanna Jaromin ◽

Grażyna Markiewicz-Łoskot ◽

Lesław Szydłowski ◽

Agnieszka Kulawik

Keyword(s):

Ventricular Arrhythmia ◽

Ventricular Arrhythmias ◽

Clinical Symptoms ◽

Diagnostic Value ◽

Control Group ◽

Healthy Children ◽

T Wave ◽

Electrical Instability ◽

Qt Intervals ◽

Wave Morphology

Background: The changes in the period of ventricular repolarization, i.e., QT interval, QTp (Q-Tpeak) and TpTe interval (Tpeak–Tend), make it possible to assess the electrical instability of the heart muscle, which may lead to the development of life-threatening ventricular arrhythmia. The aim of the study was to determine and evaluate the use of differences in T-wave morphology and durations of repolarization period parameters (QT, TpTe) in resting ECGs for children with ventricular arrhythmias. Methods: The retrospective analysis was made of the disease histories of 80 examined children with resting ECGs, which were admitted to the Children’s Cardiology Department. The study group consisted of 46 children aged 4 to 18 with ventricular arrhythmias and the control group consisted of 34 healthy children between 4 and 18 years of age, with no arrhythmias. Results: The duration of the TpTe interval was significantly (p < 0.001) longer in the group of children with ventricular arrhythmia with abnormal T-wave (bactrian/bifid, humid/biphasic) compared to the TpTe interval in children with ventricular arrhythmia with the normal repolarization period. The duration of the TpTe (p < 0.001), QTcB (p < 0.001) and QTcF (p < 0.001) intervals were significantly longer in the group of children with ventricular arrhythmias and with abnormal T-wave compared to the values of the TpTe, QTcB, and QTcF intervals of the control group with normal morphology of the repolarization period. Only the duration of the TpTe interval was significantly (p = 0.020) longer in the group of children with ventricular arrhythmia without clinical symptoms. Conclusions: Children with benign ventricular arrhythmias recorded on a standard ECG with prolonged TpTe and QT intervals and abnormal T-wave morphology require systematic and frequent cardiac check up with long term ECG recordings due to the possibility of future more severe ventricular arrhythmias. Further follow-up studies in even larger groups of patients are necessary to confirm the values of these repolarization parameters in clinical practice.

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Exocrine pancreatic dysfunction in children with obesity: characteristics of clinical picture and diagnostics

Likarska sprava ◽

10.31640/jvd.1-2.2020(5) ◽

2020 ◽

pp. 36-41

Author(s):

I. S. Lembryk ◽

O. V. Tymoshchuk

Keyword(s):

Abdominal Pain ◽

Clinical Symptoms ◽

High Density Lipoproteins ◽

Exocrine Function ◽

Control Group ◽

Type I ◽

Healthy Children ◽

Obese Children ◽

Pancreatic Lesion ◽

Functional Changes

Introduction. During the last twenty-five years the occurrence of obesity in children and teenagers has increased significantly. Materials and methods. 110 adolescents of 12–17 years old, with alimentary-constitutional obesity and involvement of pancreas and without its injury, as compared to the 30 healthy children of control group, were examined. The research provided determination of the sizes and elasticity of pancreas. The detection of the total cholesterol, high density lipoproteins and leptin level was carried out. Intensity of clinical symptoms (spastic pain in epigastrium and left subcostal arc; abdominal pain without localization, vomit without relief, general weakness) have been made accordingly to sum of points (from 0 to 3 points). If sum of points makes from 0 to 2 – intensive character of abdominal pain is low, from 3 to 5 points – it is high, from 5 to 10 points – it is very high. Normal level of amylase in a blood serum (Karavey’s method) is 12–32 g/(hour l), аnd diastase in urine – 20–160 g/(hour l). We made a screening test for elastase-I level by ELISA test. Normal activity of this enzyme in feces is 200 мkg/g. We have used sonographic method for detection of pancreatic diseases in obese children due to echo-structure of parotid gland. The analysis and statistical data processing were made by computer program "Statistica 7.0" and MS Excel XP. Research data. Physical inspection of our patients confirms prevalence of І degree over II degree obesity (52.7 % and 47.3 %, respectively; Р < 0.05). We have confirmed valid risk factor of pancreatic lesion in obese children – presence of diabetes mellitus type I in close relatives (80 % and 65 %, χ2 = 2,05; Р < 0.05). The changes of exocrine function of the pancreas in children with the stage II obesity were established. Echographic signs of the pancreas lesion in teenagers with obesity indicate the presence of functional changes: edema of head or entire edema, partial increase of parenchymal echogenicity, insufficient enlargement of the duct of Wirsung.

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Th Cytokine Profile in Childhood-Onset Systemic Lupus Erythematosus

10.21203/rs.3.rs-130849/v1 ◽

2021 ◽

Author(s):

Wei Quan ◽

Jingnan An ◽

Gang Li ◽

Guanghui Qian ◽

Meifang Jin ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Disease Activity ◽

Lupus Erythematosus ◽

Peripheral Blood ◽

Healthy Control Group ◽

Control Group ◽

Healthy Children ◽

Childhood Onset ◽

Systemic Lupus ◽

Healthy Control

Abstract Background: Childhood-onset systemic lupus erythematosus (cSLE) is a kind of chronic inflammatory disease characterized by a highly abnormal immune system. This study aimed to detect expression of the Th cytokines IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-21, IL-22, IFN-γ and TNF-α in the peripheral blood of children with cSLE; clinical symptoms; and a disease index and discuss the relationship between the Th cell cytokine regulatory network and onset of systemic lupus erythematosus (SLE) in children and disease outcome.Methods: A total of 33 children with cSLE and 30 healthy children were enrolled in this study. Children in the cSLE group were classified into the inactive cSLE group or active cSLE group according to their SLE disease activity index 2000 (SLEDAI-2K). Th cytokine profiles in peripheral blood of different groups were detected and analyzed.Results: The levels of IL-2, IL-10 and IL-21 in the cSLE group were significantly higher than those in the healthy control group (P < 0.05, P<0.01 and P<0.01, respectively). The expression of IL-2, IL-10 and IL-21 in the active cSLE group was significantly higher than that in the healthy control group (P<0.05, P<0.01 and P<0.05, respectively), but IL-22 expression was remarkably lower in the active cSLE group than in the healthy control group (P<0.001). IL-21 in the inactive SLE group was significantly higher than that in the healthy control group (P<0.05). The levels of IL-2 and IL-10 in the active cSLE group were significantly higher than those in the inactive cSLE group (P<0.01 and P<0.05). In-depth analysis showed that the expression levels of IL-2 (r=0.382, P=0.028), IL-6 (r=0.514, P=0.002) and IL-10 (r=0.429, P=0.016) were positively correlated with disease activity. Conclusion: This study provides a theoretical basis for the discovery of effective methods to regulate imbalance in T lymphocyte subsets in cSLE, which may open up potential new approaches for the diagnosis of cSLE.

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Tympanometry and computed tomography measurement of middle ear volumes in patients with unilateral chronic otitis media

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20211181 ◽

2021 ◽

Vol 7 (4) ◽

pp. 609

Author(s):

Anuj Kumar Shukla ◽

S. K. Kanaujia ◽

Sandeep Kaushik ◽

Nishant Saurabh Saxena

Keyword(s):

Computed Tomography ◽

Otitis Media ◽

Middle Ear ◽

Clinical Symptoms ◽

Chronic Otitis Media ◽

Volume Measurement ◽

Mean Value ◽

Control Group ◽

Measured Volume

Background: Tympanometry and computed tomography (CT) measurement of middle ear volume in patients with unilateral chronic otitis media.Methods: The prospective study was conducted on 50 patients of diagnosed unilateral chronic otitis media showing clinical symptoms which affect quality of life. Patients who were fulfilling inclusion criteria after screening were selected for study. We studied patients who had a unilateral tympanic membrane (TM) perforation and a normal TM in the contralateral ear which act as control group to estimate the ME volume in the lesioned ear. Further we have compared pre and postoperative middle ear volume (measured by CT and tympanometry) in control & diseased ear to correlate middle ear volume results obtained by CT and Tympanometry.Results: The mean value of MEV measured by tympanometry and CT were (1.343±0.580) and (1.106±0.380) respectively (Figure 1). Volume measurement by tympanometry is higher as compared to volume measured by CT in lesioned ear, hence tympanometry measured volume in diseased ear were higher that measured by CT which is closer to normal values in both normal and Diseased ears. Also hearing shows improvement in diseased ear after surgery due to restoration of middle ear volume closer to normal.Conclusions: It was concluded in our study that CT is more reliable investigation for middle ear volume measurement as compared to Tympanometry in diseased as well as normal ears.

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Structural-functional state of erythrocytes in children with chronic kidney disease

Medical alphabet ◽

10.33667/2078-5631-2019-1-7(382)-105-108 ◽

2019 ◽

Vol 1 (7) ◽

pp. 105-108

Author(s):

A. V. Sabirova ◽

D. K. Volosnikov ◽

O. V. Matyash

Keyword(s):

Chronic Kidney Disease ◽

Kidney Disease ◽

Red Blood Cells ◽

Functional State ◽

Peripheral Blood ◽

Blood Cells ◽

Early Stage ◽

Control Group ◽

Healthy Children ◽

Range Analysis

During Chronic Kidney Disease (CKD) development and progression emphasis is placed on structural-functional state of red blood cells. The aim of this paper is to examine red blood values and red blood cells morphological type in peripheral blood among children with CKD. 75 children with CKD aged 5-16 years were examined. The control group consisted of 25 healthy children of the same age range. Analysis of the morphometric parameters of red peripheral blood cells - mean volume (MCV) and erythrocyte diameter, mean content (MCH) and mean hemoglobin concentration in the erythrocyte (MCHC), were performed using a Gobas Micros (Roche) hematology counter. Smears were marked according to Romanovsky-Giemsa. Morphometric studies of red blood cells were carried out using the Morphology 5.2 program and red blood cell confocal microscopy on a LSM-710 Confocal Microscope, manufactured by Carl Zeiss. The tendency to poikilocytosis in CKD was confirmed. We identified that among children with CKD the distribution of red blood cells in diameter is disturbed, the content of microcytes increases, poikilocytosis is observed with an increase in the content of irreversibly transformed elements. The revealed features may serve as an additional criterion for the diagnosis of chronic kidney insufficiency at the early stage.

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The Effects of Iron Deficiency on Neutrophil/Monocyte Oxidative Burst Response in Children.

Blood ◽

10.1182/blood.v108.11.3722.3722 ◽

2006 ◽

Vol 108 (11) ◽

pp. 3722-3722

Author(s):

S.G. Berrak ◽

Meryem Angaji ◽

Emine Turkkan ◽

Cengiz Canpolat ◽

Cetin Timur ◽

...

Keyword(s):

Iron Deficiency ◽

Oxidative Burst ◽

Iron Supplementation ◽

Deficiency Anemia ◽

Control Group ◽

Healthy Children ◽

Phagocytic Cells ◽

Functional Studies ◽

Oxidative Burst Response ◽

Group 1

Abstract Background: Microbial killing killing of by phagocytic cells by iron containing myeloperoxidase pathway is part of the immune system. We aimed to investigate the effects of iron deficiency anemia (IDA) on oxidative burst response of phagocytic cells and understand if the effect is reversible after iron supplementation. Material and methods: There were 57 IDA patients and 31 healthy children as the control group that were aged between 6 months-12 years with similar demographic status. IDA patients were classified according to the severity of their anemia as follows; group 1 (Hb<8 gr/dl), group 2 (Hb≥8 gr/dl and Hb≤10 gr/dl), and group 3 (Hb>10 gr/dl). Neutrophil and monocyte oxidative burst response of each three groups of IDA and control group were compared by flow cytometry. IDA group were given oral iron supplementation (3 mg/kg-Ferrosanol) therapy. On day 15 of iron supplementation therapy neutrophil and monocyte oxidative burst response of each three groups of IDA were rechecked and compared to the control group. In order to minimize the discrepancies observed in functional studies, the oxidative burst index was calculated as the proportion of oxidative burst response in IDA group to the simultaneous oxidative burst response in the control group. Results: The number of infection episodes observed in the last three months was significantly higher in the IDA group (0–13 episodes) than the control group (1–2 episodes) (p=0.05). Neutrophil (p=0.1) and monocyte (p=0.02) oxidative burst responses in IDA group were found to be less than the control group. On day 15 of iron supplementation therapy, these values were observed to increase to the control group’s values. Evaluation of neutophil and monocyte burst indexes at the diagnosis of IDA revealed that the neutrophil burst indexes in the Hb ≤10 gr/dl group (p=0.006), and monocyte burst indexes in the Hb <8 gr/dl group (p=0.01) were significantly low. The initially observed significant differences in oxidative burst responses disappeared at day 15 of iron supplementation therapy in all hemoglobin groups. Conclusion: Correction of the differences in oxidative burst response after iron supplementation therapy implies the fact that IDA might be the reason for changes in neutophil and monocyte burst indexes.

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The effects of adenoidectomy of serum insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children with otitis media with effusion

Otolaryngologia Polska ◽

10.5604/01.3001.0014.0223 ◽

2020 ◽

Vol 74 (4) ◽

pp. 13-17

Author(s):

Beata Żelazowska-Rutkowska ◽

Klaudia Jacewicz ◽

Edwina Kasprzycka ◽

Bożena Skotnicka ◽

Bogdan Cylwik

Keyword(s):

Growth Factor ◽

Otitis Media ◽

Otitis Media With Effusion ◽

Reference Group ◽

Serum Insulin ◽

Serum Levels ◽

Control Group ◽

Healthy Children ◽

Insulin Like Growth Factor ◽

Significant Difference

Aim: The aim of the current study was to assess the serum levels of insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children suffering with or without otitis media with effusion before and after adenoidectomy. Material and methods: Serum IGF-1 and ghrelin concentrations were measured with specific enzyme-linked immunoassay (ELISA) methods. The study was carried out in 20 children with otitis media with effusion. The reference group comprised 24 children with hypertrophied adenoid, while control group included 19 children. Results: This mean values of IGF-1 in children with otitis media with effusion and children with hypertrophied adenoid before adenoidectomy were significantly lower than those found in healthy children. Serum levels of IGF-1 were higher after adenoidectomy. There was a significant difference of serum ghrelin levels between both examined groups and the control group. Conclusion: Our results suggest that adenoidectomy in children with hypertrophied adenoids and in children with otitis media with effusion significantly increases the level of IGF-1 in serum compared to before surgery through the effect of the GH-IGF-1 axis, which could contribute to children’s growth.

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AB0745 BONE STATUS IN CHILDREN WITH JUVENILE IDIOPATHIC ARTHRITIS (JIA) ON T2T THERAPY

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.3632 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1401.1-1401

Author(s):

N. Shevchenko ◽

Y. Khadzhynova ◽

L. Bohmat

Keyword(s):

Vitamin D ◽

Bone Tissue ◽

Clinical Manifestations ◽

Ionized Calcium ◽

Coefficient Of Determination ◽

Normative Values ◽

Control Group ◽

Healthy Children ◽

Basic Therapy ◽

Active Joints

Background:In recent years approaches to both the diagnosis and treatment of JIA have changed. Among it key positions for bone health are reducing the time to make a diagnosis and initiation of basic therapy, as well as reducing the timing and doses of corticosteroids. The interdependence of osteopenia in children with JIA with established impairment of vitamin D status, the prevalence of which remains very high in this cohort of patients, has also not been definitively elucidated.Objectives:To study the condition of bone tissue in children with JIA in modern disease management and to identify adverse factors for the development of osteopenia among the clinical manifestations signs.Methods:The study included 35 children with JIA aged 7 to 17 years, mostly female (77.1%), with oligo - (25.7)%, poly - (60.0%) and undifferentiated (14.3 %) arthritis, 53.4% have not yet received basic therapy. No corticosteroid therapy was available. The study of bone tissue was performed by two-energy X-ray absorptiometry (Explorer QD W-Hologic). All patients were tested for serum parathyroid hormone (PTH), the level of 25-hydroxyvitamin D [25(OH)D], the total and ionized calcium and blood phosphorus. The control group consisted of 12 healthy children of the same age with a normal level of 25 (OH) D.Results:The average level of 25(OH)D was 20.41±1.35 ng/ml, which is considered insufficient and was lower than in the control group (30.03±2.53 ng/ml, p<0.05). Studies of calcium and phosphorus in the blood showed that children with JIA have no deviations from the normative values. The majority of examined patients (98.37%) had normal values of PTH, in average was 30.43±0.90 pg/ml. The PTH was significantly different in children with different variants of JIA, the highest content was recorded in non-differential arthritis (34.33±1.80 pg/ml), the lowest - in the oligoarticular variant (28.36±1.43, p<0,05). PTH had a negative correlation with 25(OH)D status (r = -0.41; p <0.05) and did not depend on the sex and disease activity.The incidence of osteopenia was 28.57%, its prevalence was almost the same in different variants of arthritis and did not depend on the sex and age of patients, positivity for RF. Osteopenia was more common in ANA-positive than in ANA-negative variant (46.15% vs. 18.18%; pφ<0.05). In 30.0% patients with osteopenia decreased BMD reached the values of osteoporosis (more than 2.5σ). This group consisted of girls with predominantly polyarticular and undifferentiated JIA, with RF-negative and ANA-positive variant.Deviations from the age normative values of BMD (Z-score) were associated with BMI (r=0.33; p <0.05), JADAS scale results (r=0.35; p<0.04), the number of active joints (r=0.34; p<0.05), ANA (r=-0.34; p<0.05). In children with osteopenia it was correlated with the duration of the disease (r=-0.67; p<0.05), the number of active joints (r=-0.62; p<0.05), blood phosphorus level (r=0.74; p<0.05) and JADAS scale results (r=0.59; p<0.05). In children with preserved BMD correlations was supplemented by 25(OH)D status (r=-0.33; p<0.05) and BMI (r=-0.40; p<0.05). Analysis of the interdependence of the formation of osteopenia and clinical manifestations signs established the total significance of the main parameters of the disease with the highest coefficient of determination in the polyarticular variant: -112.65 + 0.09(patient’s age, mo)+4.33 (patient’s sex)-3.74 (ANA, units)-1.80 (RF, units)+0.01 (age of onset, months)-0.34 (number of affected joints)-0.18 (number of active joints)-0.18 (ESR, mm/h)+71.58 (ionized calcium, mmol/l)+5.19 (phosphorus, mmol/l)-0.01 (PTH, ng/ml)+0.18 (25(OH)D, ng/ml)(R2=99.99%; p<0.001).Conclusion:With modern JIA management a quarter of children have osteopenia. The state of the bone tissue is more associated with the prevalence, immunological and inflammatory activity of the disease against the background of preserved calcium-phosphorus homeostasis and vitamin D deficiency, and is not associated with the present therapy.Disclosure of Interests:None declared

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