scholarly journals Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women

2021 ◽  
Vol 23 (1) ◽  
pp. 63-72
Author(s):  
E. V. Kolesnikova ◽  
A. V. Zharov ◽  
G. A. Penzhoyan
Keyword(s):  
Plasma Concentrations ◽  
Evaluation Criteria ◽  
Clinical Manifestations ◽  
Positive Influence ◽  
Control Group ◽  
Skin Thickness ◽  
Maximal Increase ◽  
Before And After ◽  
Plasma Cytokines ◽  
Clinical Variants

Our aim was to evaluate diagnostic and pathogenetic significance of plasma cytokines (IL-20, IL-23, IL-10, TNFα, IFNγ) in the patients with various clinical and histological variants of sclerotic lichen and to assess opportunity for their use as effectiveness criteria of immunotherapy for this disease using a drug based on eukaryotic deoxyribonucleic acid (Derinat). The prospective cohort study included assessment of the clinical manifestations (itching and dyspareunia) and measurement of blood cytokine contents (IL-20, IL-23, IL-10, TNFα, IFNγ) in women (n = 114) with various clinical variants of sclerotic lichen (atrophic, sclerotic and sclerotic-atrophic) before and after immunotherapy with a nucleic acid-based drug (Derinat). Derinat was chosen due to the fact of being an agonist of Toll-like receptors, and a number of immunoregulatory effects, including the ability to modulate cytokine production and to exert a positive influence upon regeneration processes. In addition, based on visual inspection, vulvoscopy and morphohistochemical examination results (evaluation criteria: skin thickness, number of collagen fibers, severity of fibrosis and sclerosis, etc.), the corresponding subgroups were classified within the II group, i.e., 2.1 (minimal sclerotic signs, n = 14), and 2.2 (pronounced sclerotic signs, n = 20). The control group consisted of conditionally healthy women, without history or presence of vulvar pathology (n = 30), with an age ranging from 20 to 50 years. Along with cytokine assessment by enzyme immunoassay, the study used the data of clinical examination (anamnesis collection, examination, palpation, vulvoscopy), as well as complex morphohistochemical evaluation of vulvar tissues. In atrophic variant, we have observed an increase in plasma IL-23 content, along with decreased TNFα; in lichen sclerosis, a maximal increase in IL-20, IL-23, and IFNγ was revealed; in sclerotic form of sclerotic lichen variant with severe sclerotic features, maximally enhanced IL-20, IL-23, TNFα, IFNγ, along with minimal levels of IL-10 was registered, as compared with other groups. Immunotherapy using Derinate resulted into significant reduction in the clinical manifestations in sclerotic lichen, i.e., itching of the vulva and dyspareunia, as well as normalization of cytokine indexes. Our studies have demonstrated an opportunity of using plasma concentrations of IL-20, IL-23, IL-10, TNFα, IFNγ as biomarkers of sclerotic lichen variants, and as laboratory criteria for efficiency of immunotherapy.

scholarly journals Hyperthyroidism-Associated Insulin Resistance Is Not Mediated by Adiponectin Levels

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Chih-Hsun Chu ◽  
Hing-Chung Lam ◽  
Jenn-Kuen Lee ◽  
Chih-Chen Lu ◽  
Chun-Chin Sun ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Plasma Concentrations ◽  
Serum Glucose ◽  
Antithyroid Drugs ◽  
Control Group ◽  
Model Assessment ◽  
Insulin Levels ◽  
Significant Difference ◽  
Before And After ◽  
Antithyroid Treatment

To evaluate the relationship between circulating adiponectin and insulin sensitivity in patients with hyperthyroid Graves' disease, we studied 19 adult patients with this disease and 19 age- and sex-matched euthyroid controls. All hyperthyroid patients were treated with antithyroid drugs and were re-evaluated after thyroid function normalized. Before antithyroid treatment, the adiponectin plasma concentrations were not different comparing with those in control group. The adiponectin levels remained unchanged after treatment. The homeostasis model assessment of insulin resistance (HOMA-IR) in hyperthyroid group was higher before treatment than after treatment. There was no significant difference in serum glucose and insulin levels between hyperthyroid and control groups and in the hyperthyroid group before and after treatment. BMI-adjusted adiponectin levels were not different among three groups. On the other hand, BMI-adjusted insulin levels and HOMA-IR values were significantly decreased after management of hyperthyroidism. Pearson's correlation revealed that insulin and HOMA-IR values positively correlated with triiodothyronine (T3) and free thyroxine (FT4) levels. However, adiponectin did not correlate with T3, FT4, insulin, HOMA-IR and thyrotropin receptor autoantibody (TRAb) levels. In conclusion, insulin resistance associated with hyperthyroidism is not mediated by the levels of plasma adiponectin.

scholarly journals 1% ivermectin in combination therapy regimens for rosacea

2020 ◽  
pp. 17-23
Author(s):  
E. A. Khlystova ◽  
A. L. Savastenko
Keyword(s):  
Quality Of Life ◽  
Comparative Analysis ◽  
Combination Therapy ◽  
Combination Treatment ◽  
Clinical Manifestations ◽  
Life Quality ◽  
Clinical Results ◽  
Control Group ◽  
Before And After

Introduction. The article provides latest data on modern methods of treating rosacea. The results of own clinical observations of patients with moderate to severe papulopustular rosacea receiving combination treatment and a comparative analysis of the efficacy of various therapy regimens are presented.Objective of the study. The aim of the study was to conduct a comparative analysis of the therapeutic efficacy of combination therapy using the ivermectin 1% topical drug combined with systemic therapy drugs (doxycycline, minocycline, isotretinoin).Materials and methods. We observed 37 patients with moderate to severe papulo-pustular rosacea subtype. The patients were divided into 4 groups (A, B, C, D). Patients in the control group received monotherapy with 1% ivemectin topical drug, patients in the other groups received combination therapy (1% ivermectin combined with low-dose doxycycline, minocycline and isotretinoin). The efficacy of the therapy was evaluated by measuring rosacea severity on the Scale for Diagnostic Assessment of Rosacea (SDAR), clinical manifestations according to the IGA (Investigator Global Assessment) criteria, and by assessing the patients' quality of life using the DLQI (Dermatology Life Quality Index) questionnaire before and after 3-month treatment.Results. The comparative analysis of changes in severity indicators of the skin process and quality of life in patients with moderate to severe papulopustular rosacea after topical and combination therapy showed that the results of the treatment in patients receiving combination therapy were more significant than those in the group receiving monotherapy.Conclusion. The concomitant use of 1% ivermectin and systemic drugs is most effective in patients with severe papulopustular rosacea subtype. The combination treatment tailored to the clinical forms and severity of rosacea allows to optimize the clinical results of the therapy, which significantly affects the patients' quality of life and opens up potential for an individual approach in the algorithms for the treatment of rosacea.

scholarly journals The role of plant stanols in the primary prevention of hypercholesterolemia in patients with arterial hypertension

2019 ◽  
Vol 18 (3) ◽  
pp. 5-10
Author(s):  
A. S. Safaryan ◽  
T. V. Kamyshova ◽  
D. V. Nebieridze ◽  
V. D. Sargsyan
Keyword(s):  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Clinical Manifestations ◽  
Hdl Cholesterol ◽  
Average Level ◽  
Control Group ◽  
Study Group ◽  
Plant Stanols ◽  
Before And After ◽  
Lipid Parameters

Aim. To assess the efficacy and safety of plant stanols in patients with arterial hypertension (AH) and disorders of lipid metabolism, without clinical manifestations of atherosclerosis.Material and methods. The study included 40 patients — men at the age of 50-55 years, with AH (stage 1) and cholesterol above 5,0 mmol/l and LDL cholesterol above 3,0 mmol/l with low and medium risk (SCORE — 1-4%). Patients with coronary artery disease and other clinical manifestations of atherosclerosis, secondary hypertension, liver diseases, diabetes mellitus, secondary dyslipoproteinemia, cardiovascular diseases requiring constant therapy, bronchial asthma were excluded from the study. Patients were randomized into 2 groups of 20 people. In the study group of patients within 3 months received plant stanols (2 tab./day), in the control group — only healthy diet. In addition, patients with high blood pressure received antihypertensive therapy. Assessment of lipid parameters (total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides) was performed at baseline and after 3 months. At the beginning of the study, after 3 months of the study patients underwent a full medical examination. Safety control of plant stanols was carried out by determination of liver enzymes (aspartic transaminase (AST), alanine transaminase (ALT)), before and after the completion of the study.Results. Patients of two groups were comparable in baseline characteristics (lipid parameters, blood pressure level). After 3 months of research beginning patients from study group have statistically significant decrease of total cholesterol and LDL cholesterol. The average level of total cholesterol before treatment was 5,8±0,32 mmol/l, and after treatment — 5,3±0,32 mmol/l, p<0,001. The average level of LDL cholesterol before treatment was 3,6±0,26 mmol/l, and after treatment — 3,3±0,25 mmol/l, p<0,001. According to other lipid parameters (HDL cholesterol, triglycerides) significant differences are not obtained. As a result, there was a significant reduction in the total risk from 4% to 3,1%. As for the control group, the dynamics of lipid parameters were practically not observed. For example, the average level of total cholesterol before treatment was 5,7±0,39 mmol/l, and after treatment — 5,6±0,42 mmol/l. The average level of LDL cholesterol before treatment was 3,6±0,21 mmol/l, and after treatment — 3,6±0,21 mmol/l. The differences between the study and control groups in the reduction of total and LDL cholesterol were highly significant (p<0,001). The study did not reveal the dynamics of liver enzymes. The level of AST before treatment in the study group was 28,4±6,54, and after treatment — 29,5±5,56. ALT levels were 32,3±7,38 and 33,9±5,65, before and after the study, respectively.Conclusion. The study shows the high efficacy and safety of plant stanols. We found a significant decrease of the two most important parameters of lipid metabolism — total cholesterol and LDL cholesterol.

scholarly journals Catestatin as a New Prognostic Marker in Stable Patients with Heart Failure with Reduced Ejection Fraction in Two-Year Follow-Up

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Łukasz Wołowiec ◽  
Daniel Rogowicz ◽  
Joanna Banach ◽  
Wojciech Gilewski ◽  
Władysław Sinkiewicz ◽  
...  
Keyword(s):  
Troponin T ◽  
Statistical Significance ◽  
Univariate Analysis ◽  
Plasma Concentrations ◽  
Physical Exertion ◽  
Composite Endpoint ◽  
Control Group ◽  
Reduced Ejection Fraction ◽  
Before And After

Background and Purpose. The main goal of the study was to assess the usefulness of plasma concentrations of catestatin as a predictor of a composite endpoint (CE): unplanned hospitalization and death for all causes in patients with HFrEF in the midterm follow-up. Experimental Approach. The study group consisted of 52 Caucasian patients in NYHA classes II and III. The control group consisted of 24 healthy volunteers. The biomarkers, whose concentration was assessed before and after physical exertion as well as the variability of their concentration under the influence of the physical exertion, were NT-proBNP, troponin T, and catestatin. Key Results. During the 24-month follow-up period, 11 endpoints were recorded. The univariate analysis of the Cox proportional hazard model showed a statistically significant effect of all assessed CST concentrations on the occurrence of CE. In the 24-month follow-up, where the starting concentration of catestatin was compared with other recognized prognostic factors in HF, the initial concentration of catestatin showed statistical significance in CE prognosis as the only parameter tested. Conclusions. Plasma concentration of catestatin before and after physical exertion is a valuable prognostic parameter in predicting death from all causes and unplanned hospitalization in the group of patients with HFrEF in the 2-year follow-up.

63 EQUINE FOLLICLES MODULATE CORTISOL LEVELS AND CAPABILITY OF OOCYTES TO ADAPT TO STRESS SITUATIONS

2016 ◽  
Vol 28 (2) ◽  
pp. 161
Author(s):  
D. Scarlet ◽  
N. Ille ◽  
G. D. A. Gastal ◽  
B. G. Alves ◽  
S. O. Paiva ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Plasma Concentrations ◽  
Reproductive Function ◽  
Control Group ◽  
Target Tissue ◽  
Preovulatory Follicles ◽  
Before And After ◽  
Cortisol Levels ◽  
Systemic Stress

Glucocorticoids are mediators of the systemic stress response. Acute or chronic stress characterised by high cortisol concentrations in the periphery impairs reproductive function in a variety of species and therefore may affect fertility. The ovary has been shown to be a target tissue for glucocorticoids in many species, including the mare. This study hypothesised that the equine ovary possesses internal mechanisms to modulate cortisol activity and that supraphysiologic levels of glucocorticoids do not affect oocyte IVM rates. Light horse mares (n = 9) were used in this study. Growing follicles from an induced follicular wave were divided into the following groups: G1: 5–9 mm, G2: 10–14 mm, G3: 15–19 mm, G4: 20–24 mm, and G5: ≥25 mm. Follicular fluid (FF) and compact cumulus‐oocyte complexes (COCs) were obtained by ultrasound-guided transvaginal aspiration. Blood samples were collected at the beginning and the end of every aspiration session. Cortisol (DE1887, Demeditec, Kiel-Wellsee, Germany), progesterone (ADI-901–011, Enzo Life Sciences, Farmingdale, NY, USA), and corticosteroid binding globulin (CBG, MBS047353, MyBioSource, San Diego, CA, USA) concentrations were determined by ELISA. COCs (n = 80) were randomly distributed to either the control group (DMEM-F12+ medium) or the following hydrocortisone treatment groups: 0.1 µg mL–1, 1 µg mL–1, 5 µg mL–1, 10 µg mL–1. Maturation rate was assessed 30 h after incubation. Statistical analysis was performed with the SPSS Statistics 22 software. Data were analysed using one-way ANOVA, Pearson correlation, and chi-squared test. Cortisol (115.4 ± 13.3 ng mL–1) and progesterone (22.1 ± 3.1 ng mL–1) FF concentrations were higher (P < 0.05) in G5 follicles than in all other groups, and were positively correlated (r = 0.8; P < 0.001). Plasma concentrations of cortisol (118.6 ± 7.8 v. 120.3 ± 12.2 ng mL–1), progesterone (2.4 ± 0.5 v. 2.5 ± 0.4 ng mL–1), and CBG (11.1 ± 5.1 v. 9.9 ± 3.2 µg mL–1) did not differ before and after follicle aspiration. However, plasma CBG and progesterone were negatively correlated (r = –0.56; P < 0.01). Maturation rates did not differ among groups, regardless of the hydrocortisone concentration added to the culture medium. Our results demonstrated higher cortisol concentrations in preovulatory follicles in vivo, suggesting its importance for oocyte maturation. The greater unbound cortisol available in the FF of preovulatory follicles can be indicative of the displacement of cortisol from CBG in favour of progesterone. Furthermore, equine oocytes were capable of surviving cortisol concentrations 100 times higher than those physiologically present in preovulatory follicles. This finding suggests the ability of equine oocytes to modulate cortisol levels and adapt to stress situations.

scholarly journals CORRECTION OF ANTIOXIDANT SYSTEM STATE IN PATIENTS WITH MICROBIAL ECZEMA

2019 ◽  
Vol 22 (5-6) ◽  
pp. 167-171
Author(s):  
M. M Tlish ◽  
Elena K. Popandopulo
Keyword(s):  
Antioxidant System ◽  
Standard Therapy ◽  
Metabolic Disorders ◽  
Clinical Manifestations ◽  
Pathological Process ◽  
Control Group ◽  
System State ◽  
Inflammatory Dermatosis ◽  
Before And After ◽  
Positive Effect

Antioxidant system dysfunction is a significant factor in the pathogenesis of inflammatory dermatosis. Despite an impressive number of studies on the causes and chronization of microbial eczema, the development of a comprehensive approach to therapy taking into account metabolic disorders remains a pressing task. Material and metods. In the course of this study, the effectiveness and expediency of including in microbial eczema therapy a preparation of antioxidant action of dimethyloxobutylphosphonyldimethylate (DFD) was evaluated based on the dynamics of antioxidant system dysfunction indices, markers of syndrome of endogenous intoxication and clinical manifestations. The material for the study was the blood of patients with microbial eczema taken before and after treatment. Dynamics of parameters of enzymatic and non-enzymatic units of antioxidant system dysfunction, level middle mass molecules were analyzed against the background of standard therapy and with inclusion of DFD. Results. Inhibition of antioxidant system dysfunction activity compared to the control group was detected in the blood of all microbial eczema patients prior to treatment. No antioxidant status was observed after the standard therapy course despite achieving clinical improvement. The inclusion of DFD in complex microbial eczema therapy had a positive effect on the state of antioxidant system dysfunction and the dynamics of the skin pathological process.

Effects of Repetitive Transcranial Magnetic Stimulation on Hypothalamic-Pituitary-Adrenal axis of Patients With Depression

2011 ◽  
Vol 26 (S2) ◽  
pp. 1143-1143
Author(s):  
M. He ◽  
Z. Gu ◽  
X. Wang ◽  
X. Tian
Keyword(s):  
Transcranial Magnetic Stimulation ◽  
Hpa Axis ◽  
Magnetic Stimulation ◽  
Rating Scale ◽  
Repetitive Transcranial Magnetic Stimulation ◽  
Plasma Concentrations ◽  
Control Group ◽  
Plasma Acth ◽  
Hypothalamic Pituitary Adrenal ◽  
Before And After

ObjectiveTo investigate the effects of sleep electroencephalogram-modulated repetitive transcranial magnetic stimulation (SEM-rTMS) and conventional rTMS (C-rTMS) on the activity of hypothalamic-pituitary-adrenal (HPA) axis in patients with depression.MethodsIn a double-blind, randomized controlled trial, 164 patients diagnosed with depression were randomized to treatment with SEM-rTMS (n = 57), C-rTMS (n = 55) or sham rTMS (n = 52) for 30 min every day for 10 d. Before and after treatment plasma concentrations of adrenocorticotropic hormone (ACTH) and cortisol (CORT) were measured, and the 24-item Hamilton Depression Rating Scale (HAMD-24) was used for assessment.ResultsThe HAMD-24 scores and plasma ACTH and CORT concentrations of these depressive patients before treatment were significantly different from those of the normal control group (P < 0.05). The HAMD-24 scores and plasma ACTH and CORT concentrations in the SEM-r TMS group and conventional rTMS group were decreased significantly (P < 0.05). There was a significant positive correlation between the HAMD-24 scores and plasma ACTH (n = 240, r = 0.105, P = 0.048) and CORT concentrations (n = 240, r = 0.126, P = 0.023) in the patients with depression before and after treatment.ConclusionThe antidepressant effect of rTMS, including SEM-rTMS, may be related to its decreasing HPA axis activity. (This trail was registered. No: ChiCTR-TRC-00000465).

scholarly journals Lactobacillus plantarum IS-10506 probiotic administration increases amlodipine absorption in a rabbit model

2018 ◽  
Vol 46 (12) ◽  
pp. 5004-5010 ◽  
Author(s):  
Febrina A. Saputri ◽  
Devinna Kang ◽  
Arif S. W. Kusuma ◽  
Taofik Rusdiana ◽  
Aliya N. Hasanah ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Plasma Concentrations ◽  
Rabbit Model ◽  
Control Group ◽  
Marginal Vein ◽  
Rabbit Plasma ◽  
Probiotic Supplementation ◽  
Blood Samples ◽  
Before And After ◽  
New Zealand Rabbits

Objective Probiotics are beneficial in human health. In this study, we investigated the effect of probiotics on absorption of amlodipine, a dihydropyridine calcium antagonist used in the treatment of angina and hypertension, in a rabbit model. Methods Lactobacillus plantarum IS-10506 probiotic was administered for 14 days to male New Zealand rabbits. Blood samples were collected before and after probiotic supplementation. Amlodipine (10 mg) was then administered to all groups. Blood samples from a marginal vein were withdrawn at 5, 15, 30, 60, and 120 minutes to determine amlodipine concentrations in rabbit plasma. Results Amlodipine concentrations in the L. plantarum IS-10506 group were 4.95 ± 1.22, 8.71 ± 0.69, and 12.48 ± 2.53 ng/ml, and those in the control group were 1.69 ± 0.31, 3.89 ± 1.23, and 7.17 ± 1.85 ng/ml at 30, 60, and 120 minutes, respectively after administration of amlodipine. Amlodipine concentrations in the L. plantarum IS-10506 group were significantly higher than those in the control group at 30, 60, and 120 minutes after amlodipine administration. Conclusion Our results suggested that supplementation of L. plantarum IS-10506 significantly increases amlodipine plasma concentrations in rabbits.

scholarly journals Optimization of antibacterial therapy in patients with endo-periodontal lesions

2021 ◽  
Vol 10 (1) ◽  
pp. 17-24
Author(s):  
R. A. Saleev ◽  
S. L. Blashkova ◽  
E. V. Krikun ◽  
G. T. Saleeva ◽  
J. V. Blashkova ◽  
...  
Keyword(s):  
Diode Laser ◽  
Periodontal Diseases ◽  
Evaluation Criteria ◽  
Qualitative Assessment ◽  
Periodontal Pocket ◽  
Control Group ◽  
Root Canals ◽  
Results Of Treatment ◽  
Before And After ◽  
Technological Effectiveness

To improve the quality and effectiveness of medical care for patients with inflammatory periodontal diseases, it is necessary to search for new approaches in both diagnosis and treatment. The aim of our study is to determine the effect of the diode laser on the pathogenic microflora of periodontal pockets and root canals in patients with endo-periodontal lesions (EPL). We carried out a comparative assessment of the bacterial contents of the root canals and periodontal pockets by polymerase chain reaction (PCR) before and after treatment with a diode laser (Doctor Smile Simpler, wavelength 980 nm) for 54 patients with EPL. The control group consisted of 56 patients who were treated according to the generally accepted method, including professional oral hygiene, endodontic treatment, and curettage of periodontal pockets. Evaluation criteria were a qualitative assessment of the content of periodontopathogens in the root canal and periodontal pocket before and after and treatment. As a result of our study, we found a statistically significant (p<0.01) reduction in the colonization of periodontal pockets and root canals in patients with EPL after laser decontamination. The high technological effectiveness of the methods used in combination with the low risk of complications at the stages of endodontic and periodontological treatment provides an antibacterial effect and reduces the duration of inflammation. The results of treatment of patients using a diode laser give reason to recommend their use in the dental practice.

scholarly journals Peer Counselor Training Untuk Mencengah Perilaku Bullying

2018 ◽  
Vol 1 (1) ◽  
pp. 62-69
Author(s):  
Salmiati Salmiati ◽  
Rosmawati Rosmawati ◽  
Mardi Lestari
Keyword(s):  
Statistical Analysis ◽  
Counselor Training ◽  
Positive Influence ◽  
Control Group ◽  
Research Approach ◽  
Research Subjects ◽  
Control Group Design ◽  
Before And After ◽  
Bullying Behavior ◽  
Peer Counselor

The study examines the application of peer counselor training to prevent bullying behavior. This study uses the Tru-experimental design experimental research approach. The research design used was pretest-posttest control group design with research subjects as many as 60 students, 30 students for the control group and 30 students for the experimental group. There are two instruments used: bullying questionnaire and peer counselor training questionnaire. The results obtained through questionnaire behavior on bullying behavior were analyzed using descriptive statistical analysis showing differences in student bullying behavior between before and after the implementation of peer counselor training, namely from high categories to moderate categories. While the results of inferential statistical analysis using the t-test showed a real or positive influence on the application of peer counselor training to prevent student bullying behavior.

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