scholarly journals Liver steatosis in brain death donors

2021 ◽  
Vol 23 (1) ◽  
pp. 8-14
Author(s):  
D. V. Voronov ◽  
M. G. Minina ◽  
A. K. Chomaev ◽  
I. M. Iljinsky ◽  
O. M. Tsirulnikova
Keyword(s):  
Brain Death ◽  
Liver Steatosis ◽  
Fatty Degeneration ◽  
Moderate Degree ◽  
Cold Preservation ◽  
Men And Women ◽  
Hematoxylin And Eosin ◽  
Liver Biopsies ◽  
Severe Degree ◽  
Masson Staining

Aim. To study the frequency of fatty hepatosis in liver biopsies of consecutive brain death donors before cold preservation. Materials and methods. Liver biopsies (before cold preservation) of 300 consecutive donors with brain death were studied. Histological preparations were stained with hematoxylin and eosin, and tricolor Masson staining was performed. Results. The frequency of different degrees of fat hepatosis in men and women did not differ significantly (>0.05). Fat dystrophy of hepatocytes was absent in more than half of the cases (n = 182; 60.7%). A slight degree of fatty degeneration was diagnosed in 57 (19,0%) donors. In total, 239 (79.7%) donor livers were absolutely suitable for transplantation. Moderate degree of steatosis, which is associated with early biliary complications, was detected in 18 (6.0%) cases, and severe degree, which is a contraindication to the use of the organ for transplantation, was detected in 43 (14.3%) cases. Conclusion. Before cold preservation, liver from brain death donors is relatively rarely unsuitable for transplantation.

scholarly journals Effects of Storage on Quality Traits of Sausages Made with Chicken Breast Meat Affected by Wooden Breast

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 513
Author(s):  
Rodrigo Fortunato de Oliveira ◽  
Maísa Santos Fávero ◽  
Juliana Lolli Malagoli de Mello ◽  
Fábio Borba Ferrari ◽  
Erika Nayara Freire Cavalcanti ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Chicken Breast ◽  
Moderate Degree ◽  
Breast Meat ◽  
Moisture Concentration ◽  
Severe Degree ◽  
Wooden Breast ◽  
Taste And Smell ◽  
Chicken Breast Meat

The aim of this study was to examine the effects of storage on the quality of sausages made with breast from chickens affected by wooden breast myopathy (WBM). Breast samples from male broilers slaughtered at 48 days old were used. Normal (absence of myopathy), moderate degree (hardness only in one region of the breast) and severe degree samples (hardness over the entire length of the breast) were processed into sausages and evaluated prior to storage and after being vacuum-packed and stored for 7, 14, 21 and 28 days at 4 °C. There was a decrease (p < 0.001) in pH and an increase (p < 0.001) in cooking weight loss in samples of sausages, regardless of the myopathy, after 28 days of storage. Sausages produced with chicken breast samples affected by wooden breast myopathy presented higher (p < 0.0001) moisture concentration (72% for the severe degree) and higher (p = 0.0224) protein concentration (17.27% and 17.36%, respectively, for the moderate and severe degrees) than sausages made of normal samples (70.72% and 14.32%, respectively). The results indicate that sausages produced with meat from birds moderately and severely affected by the myopathy show higher oxidative stability. Fresh sausages produced with breast meat from birds affected by wooden breast syndrome may be stored (4 °C) for up to 28 days without exhibiting the characteristic rancid taste and smell. In sensory analysis, no differences were observed between the formulations, which suggests that the consumers approved the samples regardless of the disease severity in the meat used for the making of the sausages. The current results show that chicken meat affected by wooden breast myopathy can be used for producing fresh sausages in the industry.

scholarly journals A PROSPECTIVE OBSERVATIONAL STUDY OF CLINICAL PROFILE IN ETIOLOGY OF ANEMIA IN ELDERLY-A HOSPITAL BASED STUDY IN A TERTIARY CARE CENTRE IN SOUTH INDIA

2021 ◽  
Vol 9 (09) ◽  
pp. 961-972
Author(s):  
K. Shivaraju ◽  
◽  
Karanam Sai Arun ◽  
Mandhala Saikrishna ◽  
◽  
...  
Keyword(s):  
Tertiary Care ◽  
The Elderly ◽  
Moderate Degree ◽  
Care Hospital ◽  
Tertiary Care Centre ◽  
Total Study Population ◽  
Study Population ◽  
Poor Outcomes ◽  
One Year ◽  
Severe Degree

Background: Anemia is often considered as a normal physiological process that occurs with aging. but recently since past 2 decades anemia of any degree is being recognized as significant independent contributor to morbidity mortality and frailty in elderly patients. It is easy to overlook anemia in elderly as symptoms of anemia like fatigue, SOB etc are often attributed to aging process itself. many evidences accumulated states that anemia of any degree reflects poor health and increased vulnerability to poor outcomes. Materials and methods: This study was conducted in the department of internal medicine in a tertiary care hospital over a period of one year with sample size of 362 subjects satisfying the inclusion and exclusion criteria after taking the consent form. Results: Moderate degree anaemia was the commonest around, 65.6% followed by severe degree anemia 20.1%. Easy fatigability was the commonest symptom and pallor was the commonest sign of anaemia in elderly. Anemia is not always a consequence of aging. Anemia of unexplained etiology constituted 15.97% of total study population. Conclusion: Anaemia in elderly is a challenge and has to be approached in an organized manner for appropriate diagnosis and evaluation to look into its cause and plan management to improve the quality of life of the elderly persons.An effort should always be made to reach etiological diagnosis before instituting specific therapy.

scholarly journals On the Opportunities of Using the Indices of Lipid Peroxidation – Antioxidant Defense System in the Diagnostics of Varicose Veins of Small Pelvis in Women

2020 ◽  
Vol 5 (1) ◽  
pp. 14-20
Author(s):  
L. I. Kolesnikova ◽  
A. A. Semendyaev ◽  
D. A. Stupin ◽  
M. A. Darenskaya ◽  
L. A. Grebenkina ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Varicose Veins ◽  
Antioxidant Defense System ◽  
Pathological Process ◽  
Moderate Degree ◽  
Defense System ◽  
Mild Degree ◽  
Severe Degree ◽  
Degree Of Severity

Background: The prevalence of varicose veins of small pelvic veins in women of reproductive age varies widely – from 5.4 to 80 %, due to the low specificity and low sensitivity of clinical diagnostic techniques, and the absence of biomarkers that can be used to identify the formation and progression of varicose veins of small pelvic veins in women.Aims: To identify changes in the levels of parameters of the system “lipid peroxidation – antioxidant protection” with the development of varicose veins of the pelvis in women, as well as the possibility of their diagnostic use.Materials and methods: We examined 200 women with clinical signs of varicose veins of small pelvis. Control group included 30 women without any pathology of venous system. Treatment group included 137 women with varicose veins of small pelvis: with mild degree of severity – 39 women, with moderate degree of severity – 65 women, with severe degree of severity – 33 women. We performed comparative analysis of lipid peroxidation – antioxidant defense system in women with and without varicose veins of small pelvis taking into account the severity degrees of pathological process.Results: We obtained the most informative indices for lipid peroxidation – antioxidant defense processes in blood of women with varicose veins of small pelvis: concentration of diene conjugate (DC) for the mild degree of severity of pathological process, lipid hydroperoxide (LHP), DC and malonic dialdehyde (MDA) – for the moderate and severe degree of severity, and also changes in the activity of antioxidant enzymes with increase of the severity of the disease. Assessment of the level of catalase (Cat) and glutathione peroxidase (GP) showed their highest informative value at the mild degree of severity. Concentrations of glutathione reductase (GR), glutathione S-transferase (GST) and reduced glutathione (GSH) are more statistically significant markers for severe forms of pathological process.Conclusions: Our research showed the relevance and advisability of the studying the levels of lipid peroxidation – antioxidant defense indices in women with varicose veins of small pelvis. We registered the highest diagnostic value of DC, Cat and GP in the beginning of the disease and of LHP, DC, MDA, GR, GST and GSH – at severe forms of the pathological process. We obtained reference values for DC, MDA, Cat, SOD and GP concentration that can be considered as the predictors of the development of varicose veins of small pelvis.

scholarly journals Physicochemical Properties and Consumer Acceptance of Hamburgers Processed with Chicken Meat Affected by Wooden Breast Myopathy

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2330
Author(s):  
Rodrigo Fortunato de Oliveira ◽  
Maísa Santos Fávero ◽  
Juliana Lolli Malagoli de Mello ◽  
Fábio Borba Ferrari ◽  
Erika Nayara Freire Cavalcanti ◽  
...  
Keyword(s):  
Weight Loss ◽  
Lipid Oxidation ◽  
Chicken Meat ◽  
Chicken Breast ◽  
Moderate Degree ◽  
Industrial Use ◽  
Severe Degree ◽  
Wooden Breast ◽  
Consumer Test

Considering the increased incidence of wooden breast myopathy in broilers, the intake involves no threat to human health, indefinite etiology consumer rejection by appearance in such breasts, and the lack of studies on the industrial use of wooden breast. The objective of this study was evaluating the quality of hamburgers made with chicken meat affected by wooden breast. Breast samples from broilers slaughtered at 48-days-old were used. Normal (absence of myopathy), moderate degree (hardness only in one region of the breast) and severe degree (hardness over the entire length of the breast) samples were processed for the manufacture of hamburgers whose quality analyses (color, pH, cooking weight loss, shrinkage percentage, tenderness, storage weight loss, water activity, lipid oxidation, chemical composition and consumer test) were performed on non-stored samples (Day 0), and after storing at 30, 60, 90 and 120 days at −20 °C. There was a reduction (p < 0.05) in tenderness in samples of moderate-grade hamburgers (from 161.45 N to 289.40 N) after 120 days of storage. Hamburgers produced with chicken breast samples affected by wooden breast myopathy presented higher (p < 0.0001) fat concentration (5.32 g/100 g and 5.26 g/100 g, respectively, for the moderate and severe degree) than hamburgers made of normal samples (4.45 g/100 g). Lipid oxidation values increased, which exceeded the limit of rancidity detection, independent of myopathy. The consumers equally appreciated the aroma, flavor, and texture, and rated their overall acceptance as similar regardless of the quality of chicken meat. Chicken breast hamburgers with wooden breast myopathy is a viable alternative for the poultry industry.

scholarly journals The Silybin-Phospholipids-Vitamin E Complex Protects Against Fatty Degeneration, Nitrosative and Oxidative Stress in Rat Models of Liver Steatosis

2011 ◽  
Vol 140 (5) ◽  
pp. S-985
Author(s):  
Ignazio Grattagliano ◽  
Catia V. Diogo ◽  
Domenico Ferri ◽  
David Q. Wang ◽  
Piero Portincasa
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Liver Steatosis ◽  
Fatty Degeneration ◽  
Rat Models ◽  
And Oxidative Stress

Evaluation of the Status of Copper Contamination of Surface Sediments of Jen-Gen River Estuary, Taiwan

2013 ◽  
Vol 300-301 ◽  
pp. 1381-1384
Author(s):  
Cheng Di Dong ◽  
Yao Ting Tu ◽  
Chiu Wen Chen ◽  
Chih Ming Kao ◽  
Chih Feng Chen
Keyword(s):  
Municipal Wastewater ◽  
Surface Sediments ◽  
Biological Effects ◽  
Sediment Quality ◽  
River Estuary ◽  
River Mouth ◽  
Moderate Degree ◽  
River Bank ◽  
Biological Damage ◽  
Severe Degree

Major objectives of this study are to evaluation the enrichment, accumulation, and potential biological effects of copper (Cu) in the surface sediments of Jen-Gen River estuary, Taiwan. Eleven sampling locations were installed near the mouth of Jen-Gen River to collect sediment samples for analyzing Cu. Results of laboratory analyses show that concentrations of Cu in the sediments are between 67 and 278 mg/kg with an average of 181±61 mg/kg. The spatial distribution of Cu reveals that the Cu concentration is relatively high in the boundary of the river estuary. This indicates that upstream industrial and municipal wastewater discharges along the river bank are major sources of pollution. Results from the enrichment factor (EF) analysis imply that the sediments can be characterized as minor to moderately severe degree of Cu enrichment. Results of geo-accumulation index (Igeo) analysis indicate that the sediments can be characterized as none to moderate degree of Cu accumulation. Base on the comparison with sediment quality guidelines (SQGs), the concentrations of Cu in Jen-Gen River mouth sediments may cause acute biological damage.

scholarly journals Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro

2021 ◽  
Vol 11 ◽  
Author(s):  
Ting Li ◽  
Ting Fang ◽  
Linxin Xu ◽  
Xiangyang Liu ◽  
Xiaoyu Li ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Lipid Accumulation ◽  
Liver Steatosis ◽  
Fatty Degeneration ◽  
Hepatic Lipid Accumulation ◽  
Compound C ◽  
Hepatocyte Steatosis ◽  
Autophagy Pathway

Background: Metabolic associated fatty liver disease (MAFLD), characterized by hepatic lipid accumulation and fatty degeneration, is intertwined with obesity and type 2 diabetes mellitus (T2DM). Empagliflozin is a sodium-glucose cotransporter-2 inhibitor that effectively lowers blood glucose, but its effect on MAFLD and associated mechanisms are not fully understood.Methods: Eight-week-old db/db mice, an in vivo model, were administered empagliflozin or saline intragastrically. A hepatocyte steatosis model was established by inducing HL7702 cells with high glucose and palmitic acid and then treated with or without empagliflozin. The autophagy inhibitor (3-methyladenine, 3-MA) and AMP-activated protein kinase (AMPK) activator (AICAR)/inhibitor (Compound C) were used to determine the involvement of AMPK and autophagy in the regulation of lipid accumulation by empagliflozin. Ten-eleven translocation 2 (TET2) knockdown was achieved by siRNA transfection. Hepatic steatosis was evaluated by Oil Red O staining and triglyceride quantification. Immunohistochemistry, immunofluorescence, and western blot were performed to assess protein levels.Results: Empagliflozin alleviated liver steatosis in db/db mice and reduced triglyceride content and lipid accumulation in the hepatocyte steatosis model. Empagliflozin elevated autophagy, accompanied by an increase in p-AMPK and TET2. Both 3-MA and Compound C abolished the ability of empagliflozin to induce autophagy and reduce hepatic steatosis, while these effects could be recapitulated by AICAR treatment. TET2 knockdown resulted in autophagy inhibition and lipid accumulation despite empagliflozin treatment.Conclusion: Empagliflozin improves hepatic steatosis through the AMPK-TET2-autophagy pathway. The use of empagliflozin as a treatment for preventing and treating MAFLD in patients with T2DM warrants further study.

scholarly journals The Prevalence of Liver Steatosis and Fibrosis Assessed by Vibration-Controlled Transient Elastography and Controlled Attenuation Parameter in Apparently Healthy Romanian Medical Students

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2341
Author(s):  
Robert Nastasa ◽  
Carol Stanciu ◽  
Sebastian Zenovia ◽  
Ana-Maria Singeap ◽  
Camelia Cojocariu ◽  
...  
Keyword(s):  
Medical Students ◽  
Transient Elastography ◽  
Liver Steatosis ◽  
Liver Stiffness ◽  
Moderate Degree ◽  
Advanced Fibrosis ◽  
Controlled Attenuation Parameter ◽  
Significant Fibrosis ◽  
Attenuation Parameter ◽  
Associated Risk Factors

Vibration-Controlled Transient Elastography (VCTE) with Controlled Attenuation Parameter (CAP) is used as a non-invasive method for evaluating liver steatosis and fibrosis simultaneously. In this prospective study, we aimed to assess the prevalence of liver steatosis and fibrosis, as well as the associated risk factors in Romanian medical students by VCTE and CAP score. We used a cut-off CAP score of ≥248 dB/m for the diagnosis of mild steatosis (S1), ≥268 dB/m for moderate steatosis (S2), and ≥280 dB/m to identify severe steatosis (S3). For liver fibrosis, the cut-off values were: ≤5.5 kPa, indicating no fibrosis (F0), 5.6 kPa for mild fibrosis (F1), 7.2 kPa for significant fibrosis (F2), 9.5 kPa for advanced fibrosis (F3), and 12.5 kPa for cirrhosis (F4). In total, 426 Romanian medical students (67.8% females, mean age of 22.22 ± 1.7 years) were evaluated. Among them, 352 (82.6%) had no steatosis (S0), 32 (7.5%) had mild steatosis (S1), 13 (3.1%) had a moderate degree of steatosis (S2), and 29 (6.8%) had severe steatosis (S3). Based on liver stiffness measurements (LSM), 277 (65%) medical students did not have any fibrosis (F0), 136 (31.9%) had mild fibrosis (F1), 10 (2.4%) participants were identified with significant fibrosis (F2), 3 (0.7%) with advanced fibrosis (F3), and none with cirrhosis (F4). In conclusion, the prevalence of liver steatosis and fibrosis is low among Romanian medical students.

scholarly journals Burn Case Prevalence In Dr Kariadi General Hospital Semarang From 2012 to 2014

2020 ◽  
Vol 7 (2) ◽  
pp. 77-82
Author(s):  
Lucretya Yeniwati Tanuwijaya
Keyword(s):  
General Hospital ◽  
Total Sample ◽  
Moderate Degree ◽  
Burn Patients ◽  
Burn Care ◽  
Burn Prevention ◽  
Skilled Personnel ◽  
Common Etiology ◽  
Male Patients ◽  
Severe Degree

Background: Burns is a type of trauma that requires treatment and rehabilitation, which is still difficult and requires perseverance, a high amount of costs, and trained and skilled personnel. Morbidity and mortality of burns are relatively high, especially in developing countries where burn prevention and treatment systems are inadequate. The prevalence of burns is expected to be an advantage for the development of science in further research and could help clinicians prevent and curative burn care efforts. Method: The study subjects were burned patients examined and hospitalized at Dr. Kariadi General Hospital, Semarang. Data from each sample's clinical examination results were then collected to be described based on gender, age, burns degree criteria, and etiology of burns. Results: The total sample obtained was 72 samples, including 61 patients (85.53%) with new burns and 11 non-emergency patients (14.47%). From a total of 61 new burn patients, eight female patients (13.11%) and 53 male patients (86.89%) were obtained; 10 patients (16.40%) were children (0-18 years), 50 (81.96%)were adults (18-65), and one patient (1.64%) belonged to geriatric (> 65 years) ); based on the degree of the burns, seven patients (11.48%) had a moderate degree and 54 patients (88.52%) had a severe degree; there were 28 patients (45.90%) who suffered burns caused by fire, 20 patients (32.79%) due to electricity, six patients (9.84%) caused by scald, and seven patients (11.47 %) with an unknown cause. Of the 61 new burn patients, there were five patients (8.20%) who died. Conclusion: Burn prevalence in Dr. Kariadi General Hospital, Semarang in 2012-2014 are mostly experienced by men, adults, with severe burn criteria, with the most common etiology is fire.  

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