This study was carried out in order to investigate the natural occurrence of
toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and
aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016
and used for animal feed in Serbia. A total of 22 maize samples were
collected from four different districts across the country: City of Belgrade
(nine samples), Sumadija (eight samples), Podunavlje (four samples) and
Kolubara (one sample). Toxigenic fungi were identified according to the
morphological characteristics whereas the mycotoxins contamination were
detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The
tested samples were mostly infected with Aspergillus, Fusarium and
Penicillium spp., except that one sample originated from Kolubara was not
contaminated with Aspergillus species. Fusarium graminearum was the most
common species in the maize sample from Kolubara district (60%), F.
verticillioides in the maize samples from Podunavlje (43.75%) and City of
Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from
Sumadija district (26.38%). In the analysed maize samples the presence of
Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed
the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1
(AFB1) in all the investigated samples, except that DON and AFB1 were not
recorded in the samples from Podunavlje and Kolubara districts, respectively.
The investigated samples were highly contaminated with ZEA, with incidence of
100% for the samples from Sumadija, Podunavlje and Kolubara districts and
88.89% for the samples from City of Belgrade district. In addition, the
samples contamination with DON was 100% and 22.2% for the samples from
Sumadija, Kolubara and City of Belgrade, districts, respectively. The highest
number of AFB1 positive samples was found in Sumadija district (87.5%), while
in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1
positive samples were established, respectively. Generally, remarkable
infection of all the tested samples with toxigenic fungal species from
Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high
contamination with mycotoxins ZEA, DON and AFB1 were also recorded;
nevertheless, only in one sample the level of DON exceeded the allowed legal
limit (1750 ?g kg-1) according to Regulation for unprocessed maize.
Therefore, permanent mycological and mycotoxicological analyses of maize
grain are necessary for risk assessment of fungal and mycotoxin contamination
throughout the food chain.