scholarly journals Effects of Humic Acid on the Germination Traits of Pumpkin Seeds under Cadmium Stress

2013 ◽  
Vol 5 (4) ◽  
pp. 480-484
Author(s):  
Maasoumeh ASADI ◽  
Mohammad SEDGHI ◽  
Raouf Seyed SHARIFI

The study tackled the effect of humic acid and cadmium concentrations on the pumpkin seed germination characteristics throughout were studied. Treatments were cadmium concentrations on three levels: 0, 100 and 200 ppm and humic acid concentration of 0, 100, 200, 300 and 400 mg lit-1. Results showed that interaction of humic acid and cadmium was not significant on germination traits, but there was a significant effect on seedling growth indexes. Radicle and plumule length increased by 86 and 192% in comparison with control, of the mixture of 200 ppm cadmium and 300 mg lit-1 of humic acid. Cadmium had stimulatory effect on radicle and cotyledon dry weight and the highest values obtained with 200 ppm in mixture with 200 mg lit-1 of humic acid. Also, maximum plumule dry weight was recorded in 200 ppm cadmium and 300 mg lit-1 of humic acid. The highest of indexes were observed of 200 ppm cadmium and 400 mg lit-1 humic acid. In conclusion, the humic acid had detoxifying effect on cadmium stress in the culture and responded antagonistically against cadmium, but it seems that these concentrations of cadmium are low for the pumpkin seed and can be increased in order to reach the toxicity level.

2021 ◽  
Vol 12 ◽  
Author(s):  
Hao Zhang ◽  
Mingfang Hu ◽  
Hongyuan Ma ◽  
Li Jiang ◽  
Zhenyong Zhao ◽  
...  

The period between seed germination and seedling establishment is one of the most vulnerable stages in the life cycle of annuals in the saline environments. Although germination characteristics of Suaeda salsa seeds have been reported, the comparative germination patterns of dimorphic seeds and seedling growth to different abiotic stresses remain poorly understood. In this study, germination responses of dimorphic seeds to light and temperature were compared. Meanwhile, responses of dimorphic seeds and thereafter seedlings of S. salsa to different concentrations of NaCl and Na2SO4 were also tested. The results showed that the light did not significantly affect germination percentage of brown seeds, but significantly promoted germination of black seeds. Brown seeds could reach high germination percentage over a wide temperature range, however, germination of black seeds gradually increased with the increase of temperature. Brown seeds had higher germination percentage and velocity than black seeds under the same salt conditions. However, black seeds had higher recovery germination than brown seeds when transferred to deionized water. Young seedlings had lower salt tolerance than germinating seeds. At the same concentrations, Na2SO4 had stronger inhibitory effect on seed germination and seedling growth than NaCl. This study comprehensively compared germination traits of dimorphic seeds and seedling growth of S. salsa, and then developed a conceptual model to explain their adaptation to harsh saline environment.


2021 ◽  
Vol 56 ◽  
pp. 113-127
Author(s):  
M. Zahidul Islam Hawlader TUHIN ◽  
◽  
Taufika Islam ANEE ◽  
Naznin AHMED ◽  
Mira RAHMAN ◽  
...  

An experiment was conducted to investigate the effect of different levels of cadmium (Cd) stress on germination, morphology, physiology and yield of rice. There were five treatments, viz. control (no Cd), 0.5 mM CdCl2, 1 mM CdCl2, 1.5 mM CdCl2 and 2 mM CdCl2, carried out by a randomized complete block design (RCBD) with three replications. Cadmium stress caused a significant reduction of plant height and tillers hill-1 in a dose-dependent manner. The highest decline of relative water content and SPAD value was found at 1.5 mM (11 and 16%) and 2 mM (14 and 19%) Cd stress compared to the control. The number of effective tillers hill-1, filled grains panicle-1, 1000-grain weight, length of panicle, grain yield and straw yield reduced significantly by 44, 32, 15, 24, 37 and 45%, respectively, over the control. Germination and seedling-related parameters were recorded 10 days after germination. The lowest germination percentage (27%), germination index (27%) and seedling vigour index (49%) were observed at the highest level of Cd stress in comparison to the control. Cadmium stress resulted in maximum abnormal seedlings and markedly reduced length of root and shoot as well as their fresh and dry weight. Root shoot ratio showed differential responses to Cd stress. Thus, it can be concluded that a higher level of Cd in soil has a detrimental effect on seed germination, morphology, physiology and yield of rice.


2014 ◽  
Vol 47 (3) ◽  
pp. 39-45
Author(s):  
S.A. Tabatabaei

Abstract Seed germination and seedling growth are critical stages in the life cycle of a plant, especially under adverse abiotic stresses. Seed germination negatively affected by stress conditions. Seed priming techniques have been used to increase germination characteristics and improve germination uniformity in more field crops under stressed conditions. This experimental aimed to evaluate the effect of salinity and halo-priming on seed reserve utilization and seed germination of wheat seeds. For create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, - 12 and -16 bar was used. Seeds of were pretreated with halo-priming at 10 °C for 24 h and water at 10 °C for 24 h. Our results showed that treatment×drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage was obtained from halo priming in control conditions. Thus priming improved study traits in wheat under salinity stress. Priming increased germination percentage and seed reserve utilization as compared to the unprimed seeds. The highest germination percentage and seed reserve utilization were as obtained from halo priming in control conditions.


2020 ◽  
Vol 80 (1) ◽  
Author(s):  
Parisa Sheikhzadeh ◽  
Nasser Zare ◽  
Fatemeh Mahmoudi

In this research, the effect of priming with water for 24 hours and hormone-priming with different concentration of Gibberellic acid (GA3) (50, 100, 150 mg L-1) for 24 hours and hydro and hormone priming techniques either alone or in combination on germination, growth and biochemical properties of borage seedlings under cadmium stress conditions were investigated. The results showed that cadmium stress reduces seed germination and seedlings growth indices. Seed priming led to the significant increase in the germination percentage and rate, seedling dry weight and length, seedling vigor indices, and as well as the catalase and peroxidase activity of borage seedlings under both cadmium stress and non-stress conditions. Among the priming treatments, the combination of hydro and hormone priming showed the highest effects on the improvement of germination and seedling growth under cadmium conditions, which was significantly higher than those of the individual use of the hydro or hormone priming. At all levels of cadmium stress utilization of the combined hydro and hormone priming led to the 0.9 to 11.53-fold, and 0.95 to 2.63-fold increase in the seedlings dry weight compared with the control treatment and individual use of hydro or hormone priming, respectively. The highest activity of catalase and peroxidase enzymes in borage seedling was obtained from the seeds primed with the combination of hydro priming with 150 mg L-1 GA3, which was significantly higher than those of the other treatments. Generally, at all levels of cadmium stress, the combined hydro and hormone (specially 150 mg L-1 GA3) priming had the most positive effects on seed germination, growth and biochemical properties of borage seedlings.


2018 ◽  
Vol 24 (2) ◽  
Author(s):  
SUPRIYA DIXIT ◽  
R. K. GUPTA

Currently, a real challenge for the workers in the agricultural research field is to stop or reduce the use of expensive agrochemicals/ chemical fertilizers which are hazardous to the environment as well as human health. Present study was aimed to improve the growth and obtain optimum yield of Vigna crop with eco-friendly, non-toxic way and to reduce the use of agrochemical/chemical fertilizer application in agricultural activities. A pot experiment was conducted to study the effect of chemical fertilizer (DAP) and biofertilizer ( Rhizobium strain) separately and in combination on seed germination and seedling growth (at 30 days) based on morphological parameters such as seedling length (cm), fresh weight (g), dry weight (g) and leaf area (cm)2 of Vigna radiata (L.) Wilczek. After one month (30 Days) observations, it was found that seedling length, fresh and dry weights and leaf area were maximum in T4 and minimum in T15, T7 and T8 favored improved seedling length and leaf area whereas T7, T8, and T9 favored improved fresh and dry weights as compared to control.


2019 ◽  
Vol 18 (9) ◽  
pp. 1875-1884
Author(s):  
Zhang Jun ◽  
Wang Wenke ◽  
Geng Yani ◽  
Wang Zhoufeng ◽  
Cao Shumiao

2020 ◽  
pp. 1-12
Author(s):  
E. K. Al-Fahdawe ◽  
A. A. Al-Sumaidaie ◽  
Y. K. Al-Hadithy

A pots experiment was conducted at the Department of Biology/College of Education for Girls/University of Anbar during Autumn season of 2018-2019 to study the effect of the salinity irrigation water and spray by humic acid in some of morphological, physiological, growth and yield traits of wheat cv. IPa. The experiment was randomized complete block design (RCBD) with three replications. The first factor was assigned for irrigation by saline water at four level (S0, S1, S2 and S3), while the second factor was the foliar spraying of humic acid in three level (0.0, 1.0 and 1.5 g l-1). The results showed that there was significant reduction in plant height, vegetative dry weight, biological yield and chlorophyll leaves content when the plants were irrigated by saline water approached to 41.09 cm, 0.747 g, 0.849 g plant-1 and 38.67 SPAD, respectively at salinity level of 8.3 ds m-1 compared with the plants which irrigated by fresh water. The total carbohydrates were significantly decreased at the treatment of 8.3 ds m-1 reached 18.71 mg g-1. Spray levels humic acid achieved a significant increase in plant height, dry weight of the vegetative part, biological yield and chlorophyll leaves content sprayed at 1.0 and 1.5 g l-1 compared to no sprayed. Nitrogen concentration was significantly increased, while both phosphorus and potassium were decreased in the vegetative parts of wheat as the salinity of irrigation water increased. However, the increase of humic acid levels led to significant increasing in nitrogen, phosphorus and potassium concentration.


Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 182
Author(s):  
Ruchi Bansal ◽  
Swati Priya ◽  
Harsh Kumar Dikshit ◽  
Sherry Rachel Jacob ◽  
Mahesh Rao ◽  
...  

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yankun Sun ◽  
Jiaqi Xu ◽  
Xiangyang Miao ◽  
Xuesong Lin ◽  
Wanzhen Liu ◽  
...  

AbstractAs the global population continues to increase, global food production needs to double by 2050 to meet the demand. Given the current status of the not expansion of cultivated land area, agronomic seedlings are complete, well-formed and strong, which is the basis of high crop yields. The aim of this experiment was to study the effects of seed germination and seedling growth in response to silicon (from water-soluble Si fertilizer). The effects of Si on the maize germination, seedling growth, chlorophyll contents, osmoprotectant contents, antioxidant enzyme activities, non-enzymatic antioxidant contents and stomatal characteristics were studied by soaking Xianyu 335 in solutions of different concentrations of Si (0, 5, 10, 15, 20, and 25 g·L−1). In this study, Si treatments significantly increased the seed germination and per-plant dry weight of seedlings (P < 0.05), and the optimal concentration was 15 g·L−1. As a result of the Si treatment of the seeds, the chlorophyll content, osmotic material accumulation and antioxidant defence system activity increased, reducing membrane system damage, reactive oxygen species contents, and stomatal aperture. The results suggested that 15 g·L−1 Si significantly stimulated seed germination and promoted the growth of maize seedlings, laying a solid foundation for subsequent maize growth.


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