scholarly journals The synergistic effects of hydro and hormone priming on seed germination, antioxidant activity and cadmium tolerance in borage

2020 ◽  
Vol 80 (1) ◽  
Author(s):  
Parisa Sheikhzadeh ◽  
Nasser Zare ◽  
Fatemeh Mahmoudi

In this research, the effect of priming with water for 24 hours and hormone-priming with different concentration of Gibberellic acid (GA3) (50, 100, 150 mg L-1) for 24 hours and hydro and hormone priming techniques either alone or in combination on germination, growth and biochemical properties of borage seedlings under cadmium stress conditions were investigated. The results showed that cadmium stress reduces seed germination and seedlings growth indices. Seed priming led to the significant increase in the germination percentage and rate, seedling dry weight and length, seedling vigor indices, and as well as the catalase and peroxidase activity of borage seedlings under both cadmium stress and non-stress conditions. Among the priming treatments, the combination of hydro and hormone priming showed the highest effects on the improvement of germination and seedling growth under cadmium conditions, which was significantly higher than those of the individual use of the hydro or hormone priming. At all levels of cadmium stress utilization of the combined hydro and hormone priming led to the 0.9 to 11.53-fold, and 0.95 to 2.63-fold increase in the seedlings dry weight compared with the control treatment and individual use of hydro or hormone priming, respectively. The highest activity of catalase and peroxidase enzymes in borage seedling was obtained from the seeds primed with the combination of hydro priming with 150 mg L-1 GA3, which was significantly higher than those of the other treatments. Generally, at all levels of cadmium stress, the combined hydro and hormone (specially 150 mg L-1 GA3) priming had the most positive effects on seed germination, growth and biochemical properties of borage seedlings.

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.


2019 ◽  
Vol 10 (2) ◽  
pp. 106-114
Author(s):  
Farahnaz A. Norodinvand ◽  
Davoud K. Dehkordi ◽  
Aslan Egdernezhad

Background: Deficit irrigation is an optimum technique for producing products under drought stress conditions. The superabsorbent hydrogel is a hydrophilic polymer with cross-linked 3-D hydrophilic nets that are able to take up and keep noteworthy values of water and aquatic liquids. Recently published patents have provided significant information about the superabsorbent application in agriculture. Objective: The aim of this study was to investigate the superabsorbent effect on the yield and some of the growth factors of Pisum sativum L. under drought stress conditions. Methods: The experimental factors included: irrigation treatments at two levels of 100% and 75% water requirement by the plant. The second factor included the levels of superabsorbent application which included three levels of control treatment, potting soil with a weight percentage of 0.5 and 1. The third factor was the location of the superabsorbent application. Results: According to the results, the largest root length value corresponded to I2S1U treatment plan, the largest root dry weight value corresponded to I1S2U treatment plan, the largest plant dry weight corresponded to I2S1U treatment plan, the largest grain dry weight corresponded to I2S1U treatment plan, the largest number of grains per pod corresponded to I2S2U treatment plan and the largest number of pods per plant corresponded to I2S1U treatment plan which were significant at 5% level. Conclusion: It was concluded that the presence of the superabsorbent at the lower end of the pot was effective in providing water and nutrients for the plant root.


HortScience ◽  
2018 ◽  
Vol 53 (2) ◽  
pp. 236-241 ◽  
Author(s):  
Benjamin K. Hoover

The first objective of this study was to assess the effects of coconut shell biochar in propagation substrate on seed germination and seedling growth of Coreopsis grandiflora (Hogg ex Sweet) ‘Early Sunrise’, Leucanthemum ×superbum (Bergman ex J. Ingram) ‘Silver Princess’, and Eschscholzia californica (Cham.). Cornell seed germination mix was amended with the biochar (0%, 5%, 10%, 20%, or 40%, v/v). Seed germination and seedling growth were determined during a 21-day period in two germination rooms. This particular biochar amendment did not affect final germination percentage for any of the species. All three species had seedling shoot and primary root length growth with low to moderate positive correlation (r = 0.33–0.54) with coconut shell biochar amendment volume. Coreopsis seedling dry weight was significantly higher with 40% biochar than the control (P ≤ 0.05). The second objective of the study was to compare digitally collected data with manually collected data. Two-dimensional scans of Coreopsis and Leucanthemum seedlings were collected. Seedling dry weight (mg) and seedling length (mm) predicted seedling two-dimensional area for Coreopsis (R2 = 0.73, P < 0.001) and Leucanthemum (R2 = 0.87, P < 0.001). Digitally traced shoot and root lengths were strongly positively correlated (r = 0.99–0.97) with manual ruler measurements, suggesting that digital imaging could replace manual length measurements. The results of this study suggest inclusion of this particular coconut shell biochar in seed germination, and establishment substrates can have neutral or positive effects on herbaceous perennial germination and establishment.


2021 ◽  
Vol 18 (4) ◽  
pp. 141-146
Author(s):  
Basim Y. Alkhafaji ◽  
Roaa Jafar Elkheralla ◽  
Ahmed Salman Abdulhasan

This experiment was conducted in pots to study the effects of sewage sludge application on some morphological, physiological and accumulation characters of Vigna radiata. The experiment contained the following treatments: control (C) 0 gm sludge/30 kg soil, (T1) 300 gm sludge/30 kg soil, (T2) 600 gm sludge/30 kg soil and (T3) 900 gm sludge/30 kg soil. All sludge treatments showed a significant increase in all morphological, physiological and accumulation characters compared with control. (T2 ) treatment gave a significant increase as compared to other treatments in all morphological characters (height of the plant, leaf area, and total dry weight for shoots and roots). It increased by 34.1 cm, 33.1 cm2, 29.8 gm and 3.3 gm, respectively, compared with the control. T2 treatment also gave significant values in all physiological characters (chlorophyll and protein content) as compared to other treatments and the control treatment, (2.60 μg/gm and 17.7%) respectively compared with the control. T3 treatment showed a higher accumulation of Cd and Pb in all plant parts, the root system showed greater susceptibility to bioaccumulation than the shoot system for both the heavy metals.


2013 ◽  
Vol 5 (4) ◽  
pp. 480-484
Author(s):  
Maasoumeh ASADI ◽  
Mohammad SEDGHI ◽  
Raouf Seyed SHARIFI

The study tackled the effect of humic acid and cadmium concentrations on the pumpkin seed germination characteristics throughout were studied. Treatments were cadmium concentrations on three levels: 0, 100 and 200 ppm and humic acid concentration of 0, 100, 200, 300 and 400 mg lit-1. Results showed that interaction of humic acid and cadmium was not significant on germination traits, but there was a significant effect on seedling growth indexes. Radicle and plumule length increased by 86 and 192% in comparison with control, of the mixture of 200 ppm cadmium and 300 mg lit-1 of humic acid. Cadmium had stimulatory effect on radicle and cotyledon dry weight and the highest values obtained with 200 ppm in mixture with 200 mg lit-1 of humic acid. Also, maximum plumule dry weight was recorded in 200 ppm cadmium and 300 mg lit-1 of humic acid. The highest of indexes were observed of 200 ppm cadmium and 400 mg lit-1 humic acid. In conclusion, the humic acid had detoxifying effect on cadmium stress in the culture and responded antagonistically against cadmium, but it seems that these concentrations of cadmium are low for the pumpkin seed and can be increased in order to reach the toxicity level.


2021 ◽  
Vol 11 (24) ◽  
pp. 11666
Author(s):  
Liaqat Ali ◽  
Wang Xiukang ◽  
Muhammad Naveed ◽  
Sobia Ashraf ◽  
Sajid Mahmood Nadeem ◽  
...  

Reduced germination and early crop maturity due to soil compaction, nutrients stress, and low moisture are major constraints to achieve optimum crop yield, ultimately resulting in significant economic damages and food shortages. Biochar, having the potential to improve physical and chemical properties of soil, can also improve nutrients and moisture access to plants. In the present study, a growth room experiment was conducted to assess biochar influence on maize seed germination, early growth of seedlings, and its physiological attributes. Corn cob biochar (CCB) was mixed with soil at different rates (0.5%, 1%, 1.5%, 2%, 2.5%, and 3% w/w) before seed sowing. Results obtained showed that increasing CCB application rate have neutral to positive effects on seed germination and seedling growth of maize. Biochar addition at the rate of 1.5% (w/w) significantly increased shoot dry biomass (40%), root dry biomass (32%), total chlorophyll content (a and b) (55%), germination percentage (13%), seedling vigor (85%), and relative water content (RWC) (68%), in comparison to un-amended control treatment. In addition to this, it also improved germination rate (GR) by 3% as compared to control treatment, while causing a reduction in mean emergence time (MET). Moreover, application of biochar (3%) also resulted in enhancement of antioxidant enzyme activity, particularly superoxide dismutase (SOD) and catalase (CAT) by 13% and 17%, respectively. Conclusively, biochar application is an attractive approach to improve the initial phase of plant growth and provide better crop stand and essential sustainable high yields.


2018 ◽  
Vol 9 (2) ◽  
pp. 207-213
Author(s):  
Baghdad Science Journal

The effect of saline magnetized water irrigation on seed germination and seedling growth of wheat cultivar Iraq were studied. Irrigation water was supplemented with different levels of Sodium chloride 6, 12 or 18 mmhos/ cm in addition control treatment, and passed through a proper magnetic felid with 1000, 1250, 1500 or 2000 gaus in addition control treatment. The results showed significantly stimulated shoot development and led to the increase of germination, seedling emergence, area leaf, length of shoot and root and fresh and dry weight compared to the controls. Results also showed significant interaction between saline water and magnetized water. So, using magnetic treatment of saline water could be a promising technique for Agricultural improvement.


2019 ◽  
pp. 49-61
Author(s):  
Reyhaneh Azimi ◽  
Mohammad Kia Kianian ◽  
Mohammad Pessarakli

Improvement in the rate and amount of germination of seeds has a very important effect on the establish-ment of primary seedlings and the increase of rangeland production. The rapid and uniform germination of seeds leads to the successful establishment of plants. The use of nanoscale materials can help germinate faster seeds. Therefore, in this study, the effects of TiO2 nanoparticles in concentrations of 0, 10, 20, 30, 40, 60 and 80 mg / l on the rate and speed of seed germination of Ziziphora clinopodioides Lam. paid. This design was carried out in a completely randomized design with four replications for 20 days at a constant temperature of 20°C under 12 hours of light and 12 hours of darkness at the Germinator of Natural Resources Faculty of the Ferdowsi University of Mashhad. The results showed that germination percentage of treated seeds with TiO2 nanoparticles increased to 23% ppm compared to control treatment. Also, in other concentrations of other nanoparticles, there was a positive effect on speed and germination percentage, so that the effect of different concentrations of nanoparticles on germination characteristics of Ziziphora clinopodioides Lam. seeds was significant. The highest germination percentage was observed in the concentration of 30 ppm and the lowest germination rate at 30 and 20 ppm concentrations. In high concentrations of TiO2 nanoparticles, no positive effects were observed on the germination characteristics of seed Ziziphora clinopodioides Lam. To conclude the use of TiO2 nanoparticles can be improved by improving the seed germination properties of the medicinal plant Ziziphora clinopodioides Lam. that cause increases plant’s establishment in natural areas.


2011 ◽  
Vol 5 (3) ◽  
pp. 32-38
Author(s):  
Ismail E. N. ◽  
Hassan S. M. ◽  
Mageed. D. M. ◽  
Rasheed. K. A. ◽  
Abd A. S. ◽  
...  

A Study was conducted the effect of saline water irrigation with different levels of NaCl (6, 12, 18) Decisemins/M in addition with the control treatment. The saline water magnetized through a proper magnetic field with (1000, 1250, 1500, 2000) gaus in addition control treatment. Seed germination, seedling emergence and seedling growth of Alletiffia wheat cultivar were investigated. The result indicated significant increasing in most character studies such as, seed germination, seedling emergence, shoot and root length, fresh and dry weight of shoot, root, and leaf area. On the other hand, all character studies were significantly decreased when used saline irrigation water. The result also showed significant interaction between the magnetized water and saline water which used in irrigation pots in most characters studies.


2019 ◽  
Vol 50 (Special) ◽  
Author(s):  
Burhan & AL-Hassan

This research was carried out at the fields of Field Crops Dept. Coll. of Agric. Univ. of Baghdad, during the season 2017-2018, to investigate the effect of Nano fertilizer NPK (Fn1, Fn2, Fn3: spraying with the liquid fertilizer 20%-9%-10% with 15% concentration of the levels 750:90:600, 500: 60: 400 and 250: 30: 200 mg L-1 and Fgn1, Fgn2, Fgn3: spraying the liquid fertilizer at 7.50% concentration of the same levels plus 50% of the soil Fertilizer) on flag leaf area, grain yield and some of the quality traits of three wheat varieties. Using randomized complete block design with split plot arrange with three replications. The results showed increases in grains yield (48.99%), protein percentage (27.24%), gluten ratio in flour (58.45%) and flag leaf area (38.69%), its dry weight (41.89%) , total chlorophyll content (18.33%), nitrogen (19.37%), phosphorus (44.11%) and potassium (12.03%) when fertilized with Fn1 treatment compared with the control treatment (Fg: soil Fertilizer 184 N, 39.56 P, 41.50 K kg ha-1). The Fgn1 and Fgn2 treatments gave increase in the specific weight of flag leaf at 31.38  and 11.44% respectively, also the Fgn1 and Fgn2 treatments increase in grain carbohydrate content at 15.00 and 10.55 % respectively compared with Fg treatment. Arrangement of the Fertilizers  treatments in their general progressively positive effects on growth, yield and quality parameters was Fn1>Fgn1>Fn2>Fgn2> Fn3>Fgn3>Fg. The Bhoth-158 variety gave the highest grains yield (5.25 tons ha-1) with increase ratios 13.14 and 23.82% compared with Uruq and IPA-99 varieties. The IPA-99 was superior in its flour content of highest ratio of the dry gluten (16.99%). The Bhoth-158 variety showed positive response to Nano Fertilizers  treatments especially the Fn1 treatment followed by IPA-99 variety. There was highly significant correlation between total grains yield and each of the flag leaf area, its dry weight and chlorophyll content, its time staying active and content of nitrogen, phosphorus and potassium.


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