scholarly journals Morphological Characterization of Wild and Cultured Clarias gariepinus (Burchell 1822) using Principal Component and Cluster Analyses

2016 ◽  
Vol 8 (4) ◽  
pp. 428-436 ◽  
Author(s):  
Folasade A. OLA-OLADIMEJI ◽  
Michael O. AWODIRAN ◽  
Omotayo FAGBUARO ◽  
Adetola O. AKOMOLAFE

The study was designed to describe the phenotypic variations in morphometric and meristic characters of Clarias gariepinus between two populations in different towns in Ekiti State, Nigeria. Fish samples were collected from Ero dam (wild population at Ikun Ekiti) and Christeve Aquaculture (cultured population at Iyin Ekiti) using cast net and dragging net. The samples were brought to Zoology Laboratory in Ekiti State University and measurements for morphometrics (24) and meristics (11) of each of the investigated catfish specimen were taken using standard procedures. To test the significance of morphological differences between the wild and cultured populations, all the data on morphometrics and meristics were subjected to student's t-test at 5% level of significance. Principal Component Analyses and cluster analyses were then employed to analyse the data obtained from the morphological traits using Paleontological Statistics (PAST) software. Significant differences were observed in the means of the morphometric and meristic traits. The multivariate analysis also showed differences between the studied populations.  These differences revealed the occurrence of heterogeneity between the two populations. Higher variations and sex related variations were also observed in the wild than in cultured population. The result show the potential for commercial purposes of the population. However, genetic markers can be used in further studies to confirm the phenotypic diversity observed and assess the amount of variation in these populations. 

2021 ◽  
Vol 16 (1) ◽  
pp. 13-20
Author(s):  
Popoola Omoniyi Michael ◽  
Olagunju Oluwatosin Olubunmi

AbstractIntraspecific morphological variation in Clarias gariepinus was studied in three water bodies. Twenty-three morphometric characteristics and seven meristic were used. Principal component (PC) analysis showed that morphometric traits of River Osun and Ogbese were somewhat related while that of Aago showed no relatedness, there were no difference among the meristic counts of the populations. PC1, and PC2 accounted for 93% and 4% of the variation observed in the populations. High positive correlation was observed in Aago (r = 0.872, b = 2.10) and Ogbese population while low correlation and negative allometric growth (r = 0.425 and b= 1.38) was observed in Asejire population. Cluster analysis revealed that three studied populations are separated into two major clusters, with samples from Asejire and Ogbese population found within the same cluster but different sub- clusters, similarly some of samples from Aago were also within the same cluster while samples 10 were found on a separate and major cluster. The study confirms the variability among individual species within each population, indicates the presence of genetic diversity among the populations of C. gariepinus and the Pre-anal distance and dorsal fin ray count could be employed in the identification of populations of catfish. Key words: morphometric traits, meristic characters, populations, allometric


Author(s):  
Chioma Adelieje ◽  
Edak Aniedi Uyoh ◽  
Valentine Otang Ntui ◽  
Daniel Ama-Abasi

Morphometric and genetic diversity studies were carried out on Chrysichthys nigrodigitatus sampled from middle and lower Cross River. The aim was to provide information on variations between these two populations and also augment the limited information currently available on genetic diversity in this species. A total of 79 fish samples were used for the morphometric analysis out of which 30 were used for the genetic study. Genomic DNA was extracted from caudal fin using a modified cetyltrimethylammonium bromide method and amplified using microsatellite markers.Twenty-three morphological features were studied from each fish sample out of which 19 showed significant differences (P<0.05) between the two populations. Principal Component Analysis identified head length, head width, caudal peduncle depth, standard length, preventral distance, snout length and anal fin length as key contributors to variation. Genetic analyses indicated low variability in the populations studied as evidenced by low Shannon`s information index (mean of 0.944 – 1.034), and positive coefficients of inbreeding (FIS) across both populations suggesting the presence of greater homozygosity in this species. Gene flow of 3.507 was observed between the lower and middle Cross River indicating the possibility of free mating between the two populations. The low levels of genetic diversity call for urgent management and conservation strategies to ensure long term survival of the species.


2021 ◽  
Vol 15 (1) ◽  
pp. 185-199
Author(s):  
Yédjanlognon Faustin Assongba ◽  
Jean Innocent Essou ◽  
Cossi Aristide Adomou ◽  
M.G. Julien Djego

Au Bénin des efforts scientifiques sont consentis pour la connaissance de la diversité végétale. Cleome gynandra L. (Caya blanc, Sabo en Adja, Akaya en Mahi et Semboué en Peulh) de la famille des Capparaceaes fait l'objet de cette étude à travers sa connaissance agromorphologique. La méthodologie utilisée passe par des observations et prélèvement dans la nature et jardins de case. Dix (10) pieds de la plante ont été collectés dans chaque zone climatique et un (1) pied dans le jardin botanique de l’Université d’Abomey-Calavi. En plus des observations sur la plante complète, il est procédé à l’achat des graines de C. gynandra. Des variables quantitatives (hauteur de la plante, nombre de fruits par pied, longueur et diamètre des fruits, celle des pédoncules, nombre de ramification) et variables qualitatives (couleur, pilosité de la tige, couleur des fruits et graines et forme des feuilles) ont été observées, mesurées et calculées. Le traitement des variables a été possible par usage du logiciel XLSTAT- pro Version 2008. 1.01. Les relations entre variables ont été étudiées grâce à la matrice de corrélation de Pearson (n). Une Analyse en Composante Principale (ACP), a permis de ressortir si des différences significatives à un taux de 0,05 existent entre les variétés pour les caractères étudiés. L'étude a mis en évidence une grande variabilité agromorphologique dans les trois zones climatiques du Bénin par des caractères discriminant. Des différences significatives ont été observées entre les variables des trois zones climatiques d'une part et entre les individus issus des jardins de case et ceux collectés dans la nature d'autre part. L’ACP a mis en évidence trois grands ensembles. La classification ascendante hiérarchisée montre (8) ensembles qui tiennent compte des similarités et les observations.Mots clés : Cleome gynandra L, Akaya, variété, caractères, légume feuille et République du Bénin. English Title: Morphological characterization of Cleome gynandra L. in Benin   In Benin, scientific efforts are being made for knowledge of plant diversity. Cleome gynandra L. (White Caya, Sabo in Adja, Akaya in Mahi and Semboué in Peulh) of the family Capparaceae is the subject of this study through its agromorphological knowledge. The methodology used involves observations and sampling in the nature and house gardens. Ten (10) feet of the plant were collected in each climatic zone and one (1) foot in the botanical garden of the University of Abomey-Calavi. In addition to the observations on the whole plant, the seeds of C. gynandra are purchased. Quantitative variables (plant height, number of fruits per foot, fruit length and diameter, peduncles, number of branching) and qualitative variables (color, stem hair, fruit and seed color and leaf forms) were observed, measured and calculated. Variable processing was possible using the XLSTAT-pro Version 2008 software. 1.01. Relationships between variables were studied using the Pearson correlation matrix (n). A Principal Component Analysis (PCA) revealed if significant differences at a rate of 0.05 exist between the varieties for the characters studied. The study revealed a large agromorphologicalvariability in the three climatic zones of Benin by discriminating characters. Significant differences were observed between the variables of the three climatic zones on the one hand and between the individuals from the house gardens and those collected in the wild on the other hand. The CPA has highlighted three major groups. The hierarchical ascending classification shows (8) sets that take into account similarities and observations.Keywords: Cleome gynandra L, Akaya, variety, characters, leaf vegetable and Republic of Benin.    


2019 ◽  
Vol 18 (2) ◽  
pp. 225-234
Author(s):  
Bambang Iswanto ◽  
Imron Imron ◽  
Rommy Suprapto ◽  
Huria Marnis

Lele Dumbo was used to be a superior clariid catfish ardely cultured in Indonesia. Despite its aquaculture success, there was uncertainty about its taxonomic identity, whether it belongs to an African catfish (Clarias gariepinus Burchell 1822) or a hybrid resulted from a hybridization between African catfish C. gariepinus and an Asian catfish C. fuscus. Though lele Dumbo was no longer popular, the genetic improvement program has successfully developed lele Sangkuriang strain, and that have recently been extensively cultivated in Indonesia. As a lele Dumbo strain, the identity of lele Sangkuriang is also uncertain, thus need to be verified. The present study aimed to investigate the similarity of lele Dumbo through morphometric and meristic characterizations using samples of lele Sangkuriang (collected from BBPBAT Sukabumi, BPBAT Cijengkol and PT STP) compared to those of African catfish C. gariepinus introduced from Thailand and Kenya. The characterizations were carried out through measurement of 20 standard morphometric characters and five meristic characters the data obtained were then analyzed using principal component analysis. The results suggested that the values of morphometric and meristic characters of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Likewise, the results of principal component analysis performed on morphometric and meristic characters also revealed that morphometric and meristic characteristics of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Those results revealed that biometric characteristic of both lele Dumbo and African catfish C. gariepinus was not different, thus they seem belong to the same species.     


2015 ◽  
Vol 10 (2) ◽  
pp. 91 ◽  
Author(s):  
Bambang Iswanto ◽  
Imron Imron ◽  
Rommy Suprapto ◽  
Huria Marnis

African catfish (Clarias gariepinus Burchell, 1822) has become a great important species in Indonesian aquaculture. Several strains of the African catfish have been introduced to Indonesia for aquaculture purposes, initiated by Dumbo strain from Taiwan in 1985, followed by Paiton strain from Thailand in 1998, then Egypt strain from Egypt in 2007, Masamo strain from Thailand in 2010 and later Kenya strain from Kenya in 2011. Since its introductions, there were no reports yet on their characterization studies. The present study was conducted to morphologically characterize the strains of African catfish introduced to Indonesia, i.e. Dumbo, Paiton, Egypt, Masamo, and Kenya strains. Morphometric and meristic data obtained were analyzed using Principal Component Analysis. Results of the morphometric characterization in the present study revealed that Dumbo, Paiton, Masamo, and Kenya strains were indistinguishable, while morphometric characteristic of Egypt strain was more or less different from those of the other strains. On the other hand, results of the meristic characterization suggested that meristic characteristics of all strains of the introduced African catfish were not different from each other. Therefore, to keep the genetic purity of those introduced strains, they should be properly maintained in isolated places.


2017 ◽  
Vol 38 (4) ◽  
pp. 449-459 ◽  
Author(s):  
Seyyed Saeed Hosseinian Yousefkhani ◽  
Mansour Aliabadian ◽  
Eskandar Rastegar-Pouyani ◽  
Jamshid Darvish

The genusAgamurawas previously known from four species occurring on the Iranian Plateau, but was recently revised as a monotypic genus that excluded three species. In the present study, we examined different populations ofAgamura persicamorphologically. We found thatA. persicashows geographic variation with respect to two groups, with the eastern population considered asA. cruralisandA. persicaproposed for the western population. Separation between the two populations ofA. persicawas verified based upon ANOVA results for many morphological characters, including Head Height (HH), Interorbital distance (IO), Forelimb length (FLL), Number of scales across widest part of abdomen (NSA), Loreal scales (LOS), and Number of scales that separate two adjacent tubercles (NTV). Principal Component Analysis (PCA) and Discriminant Function Analysis (DFA) based on metric and meristic characters confirmed the revision of the genusAgamura. Multivariate analysis indicated that all studied OTUs were assigned to the correct classification and have significantly different morphological characters.


2021 ◽  
Vol 2 (1) ◽  
pp. 09-018
Author(s):  
Mamndeyati Ndekimbe Uruku ◽  
Innocent Agbo Adikwu ◽  
Oyediran Olusegun Oyebola ◽  
Timothy E. Uchendu

Phenotypic Variability of Sampled Clarias gariepinus (Burchell, 1822) Population were assessed. 30 matured samples of Clarias gariepinus of different sizes were bought from artisanal fisherfolks using various fishing gears, morphometric and meristic attributes and other adaptive traits were characterized. Phenotypic heterogeneity (Coefficient of Variability > 10%) and multiple modes in Meristic and morphometric Values were assessed to imply plasticity and taxonomic complications respectively. Twenty morphometric and nine meristic attributes were measured in all the collected individuals and measured to the nearest 0.01cm, using Vernier calipers. The mean value of meristic attributes varied from 6.20±0.93 in PELFR-R to 71.40±5.64 in DFR. Coefficient of variability of the population varied from 7.89 in DFR to 54.02 in PESES-L. Heterogeneity occurred in 77.8% of meristic attributes except DFR and CFR. Multiple modal was recorded in PESES-L, while the mean value of the morphometric attributes varied from 5.20±0.82 in OPD to 64.52±5.00 in DFL. Coefficient of variability of the population varied from 7.76 in DFL to 18.07 in CFW. Heterogeneity occurred in 82.4% of morphometric attributes. Five components accounted for 78.65% of the total variation using Principal Component Analysis on morphometric attributes. Two of the extracted components had CV > 10 (41.70%, and 15.02%). However, the scree plot shows the slope of the variation to favors the five component with Eigenvalue greater than one. The studied C. gariepinus population was characterized by heterogeneity of phenotypic values and the population can be taxonomically discriminated by meristic and morpho types.


Methodology ◽  
2016 ◽  
Vol 12 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Gregor Sočan

Abstract. When principal component solutions are compared across two groups, a question arises whether the extracted components have the same interpretation in both populations. The problem can be approached by testing null hypotheses stating that the congruence coefficients between pairs of vectors of component loadings are equal to 1. Chan, Leung, Chan, Ho, and Yung (1999) proposed a bootstrap procedure for testing the hypothesis of perfect congruence between vectors of common factor loadings. We demonstrate that the procedure by Chan et al. is both theoretically and empirically inadequate for the application on principal components. We propose a modification of their procedure, which constructs the resampling space according to the characteristics of the principal component model. The results of a simulation study show satisfactory empirical properties of the modified procedure.


2020 ◽  
Vol 4 (2) ◽  
pp. 41-45
Author(s):  
Sadam Sulaiman Indabo ◽  
Rufai Zakari

Dermestes maculatus is a major pest of stored fish in Nigeria, fish is preserved with highly persistent synthetic chemicals. There have been an increasing effort at developing plant-based toxicants that are environmentally friendly. Therefore, this study was conducted to screen the leaf extract of Azadirachta indica for phytochemical constituents. The efficacy of the methanolic leaf extract of the plant against D. maculatus was also evaluated. The leaves of A. indica were obtained, processed and taken to laboratory for methanolic extraction. Phytochemical screening was carried out to identify saponins, flavonoids, alkaloids, tannins, steroids, cardiac glycosides, glycosides, triterpenes and carbohydrates using standard procedure.  Both clean and infested samples of smoke-dried Clarias gariepinus were purchased from Sabon Gari Market, Zaria. The infested samples were used as initial source of D. maculatus larvae. Range finding test was carried out prior to the bioassay. During the bioassay, clean un-infested fish samples were weighed and introduced into kilner jars and respective dosages of 0.2g, 0.4g, 0.6g and 0.8g of extract were added. Another set of fish samples without the extract were maintained as control. The experiment was arranged in Completely Randomized Design with three replicates. Fifteen larvae of D. maculatus were introduced into both treated and control containers and covered. Mortality was recorded after 24, 48, 72 and 96 hours. Data obtained revealed an increase in mortality with increasing dose and time. Mortality ranged from 1.67 to 11.00. Therefore, leaf extract of A. indica should be incorporated in the development of larvicide against D. maculatus.   


2020 ◽  
Vol 24 (5) ◽  
pp. 755-759
Author(s):  
A.A. Nwabueze ◽  
J.K. Ekelemu ◽  
O.A. Owe

The response of Clarias gariepinus to Allium sativum on growth performance and as anti-bacterial agent in Staphylococcus aureus challenge infection was evaluated. A. sativum was included at 0% (control), 1.5%, 3.0%, and 4.5% in fish diet. Twenty fish samples each were randomly distributed into four tanks (T1, T2, T3 and T4). Fish were fed twice daily at 5% body weight for twelve weeks. All fish were challenged with 0.5ml of pure culture of S. aureus. A significant (P<0.05) increase in body weight, total length and standard length was observed. A. sativum at 3.0% inclusion promoted highest growth with feed conversion ratio (1.25-1.41), protein efficiency ratio (1.72-0.56), specific growth rate (1.92-2.09), condition factor (0.83-1.99) and survival rate (60-95). Fish-fed A. sativum diet showed that 4.5% inclusion had the least S. aureus activity. Bacteria load significantly (P<0.05) decrease at week 12. Culture water had dissolved oxygen of 4.0 - 4.2 mg/L, pH 6.0 - 7.0 and temperature of 26.0-28.0oC. This study has shown that A. sativum supplemented diets enhanced growth of C. gariepinus and functioned as antibacterial agent against S. aureus. A. sativum at 3.0% inclusion in diet is recommended for better fish growth and 4.5% A. sativum for antibacterial action. Keywords: Fish, herb, growth, infection


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