PHYTOCHEMICAL SCREENING AND LARVICIDAL EFFECT EVALUATION OF AZADIRACHTA INDICA (L) LEAF EXTRACT ON DERMESTES MACULATUS (DE GEER, 1774) INFESTATION OF SMOKED CLARIAS GARIEPINUS (BURCHELL, 1822)

2020 ◽  
Vol 4 (2) ◽  
pp. 41-45
Author(s):  
Sadam Sulaiman Indabo ◽  
Rufai Zakari
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Standard Procedure ◽  
Clarias Gariepinus ◽  
Phytochemical Screening ◽  
Range Finding ◽  
Dermestes Maculatus ◽  
Randomized Design ◽  
Fish Samples ◽  
Initial Source

Dermestes maculatus is a major pest of stored fish in Nigeria, fish is preserved with highly persistent synthetic chemicals. There have been an increasing effort at developing plant-based toxicants that are environmentally friendly. Therefore, this study was conducted to screen the leaf extract of Azadirachta indica for phytochemical constituents. The efficacy of the methanolic leaf extract of the plant against D. maculatus was also evaluated. The leaves of A. indica were obtained, processed and taken to laboratory for methanolic extraction. Phytochemical screening was carried out to identify saponins, flavonoids, alkaloids, tannins, steroids, cardiac glycosides, glycosides, triterpenes and carbohydrates using standard procedure.  Both clean and infested samples of smoke-dried Clarias gariepinus were purchased from Sabon Gari Market, Zaria. The infested samples were used as initial source of D. maculatus larvae. Range finding test was carried out prior to the bioassay. During the bioassay, clean un-infested fish samples were weighed and introduced into kilner jars and respective dosages of 0.2g, 0.4g, 0.6g and 0.8g of extract were added. Another set of fish samples without the extract were maintained as control. The experiment was arranged in Completely Randomized Design with three replicates. Fifteen larvae of D. maculatus were introduced into both treated and control containers and covered. Mortality was recorded after 24, 48, 72 and 96 hours. Data obtained revealed an increase in mortality with increasing dose and time. Mortality ranged from 1.67 to 11.00. Therefore, leaf extract of A. indica should be incorporated in the development of larvicide against D. maculatus.   

scholarly journals In vitro anti-bacterial activity of Tinospora cordifolia leaf extract and its phytochemical screening

2019 ◽  
Vol 5 (2) ◽  
pp. 10-17
Author(s):  
B. Praiwala ◽  
S. Priyanka ◽  
N. Raghu ◽  
N. Gopenath ◽  
A. Gnanasekaran ◽  
...  
Keyword(s):  
Cell Division ◽  
Leaf Extract ◽  
Antibacterial Property ◽  
Standard Procedure ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Main Concern ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Screening Assay

Background: Antimicrobial resistance is the main concern worldwide to combat infectious. Over the years studies on leaf extracts Tinospora cordifolia have demonstrated the potent role its antibacterial property. The current study is an attempt to test its antibacterial property against Escherichia coli cell division. Material and methods: Phytochemical screening assay of T. cordifolia leaf extract was done using standard procedure and the results showed the presence of alkaloid, carbohydrate, terpenoid, steroid, tannin, amino acid, flavonoid and glycoside components. Results: HPLC analysis revealed the presence of berberine in T. cordifolia leaf extract. Further E. coli cells were treated with berberine to study its efficacy in inhibiting cell division. Antibacterial assay was performed by using disc diffusion method. Conclusion: Among aqueous, methanolic, ethanolic, chloroform, hexane and acetone extract only methanolic extract showed zone of inhibition.

scholarly journals Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura

2019 ◽  
Vol 1 (1) ◽  
pp. 20
Author(s):  
Hedi Paramita ◽  
Lindung Tri Puspasari ◽  
Yusup Hidayat ◽  
Rika Meliansyah ◽  
Danar Dono ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Development Time ◽  
Feeding Activity ◽  
Seed Extract ◽  
Control Treatment ◽  
Pests And Diseases ◽  
Randomized Design ◽  
Kalanchoe Pinnata ◽  
And Control

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.

scholarly journals Phytochemical Screening of Selected Botanicals and their Effectiveness Against Maize Weevil (Sitophilus Zeamais Motsch.) at Paklihawa, Rupandehi, Nepal

2021 ◽  
Vol 11 (1) ◽  
pp. 34-44
Author(s):  
Susmita Gautam ◽  
Subodh Khanal ◽  
Dipak Khanal ◽  
Saroj Raj Mishra ◽  
Sunil Ghimire
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Cost Effective ◽  
Storage Condition ◽  
Sitophilus Zeamais ◽  
Phytochemical Screening ◽  
Maize Weevil ◽  
Justicia Adhatoda ◽  
Artemisia Dubia ◽  
Sitophilus Zeamais Motsch

Laboratory experiment was done to perform the phytochemical screening of six locally available botanicals (Azadirachta indica Juss, Artemisia dubia Wall, Lantana camara L, Parthenium hysterophorus L, Justicia adhatoda Linn. and Moringa oleifera L.) and their effectiveness to control the maize weevil (Sitophilus zeamais Motsch.) at Agriecology lab of Institute of Agriculture and Animal Science, Paklihawa Campus, Rupandehi, Nepal. Seven treatments (leaf extract of A. indica, A. dubia, L. camara, P. hysterophorus. J. adhatoda and M. oleifera and distilled water as control) applied at 3 different doses (0.5, 1 & 2g/50g seeds) were arranged in CRD design with 3 replications. Result of mortality test of maize weevil revealed that, at highest concentration of L. camara powder (T17- 2g/50 gm seeds) mortality of maize weevil was observed up to 85% at 45 DAT. Whereas number of damaged grain was lowest in T17 (L. camara powder- 2g/50 gm seeds). The effectiveness of botanicals was found to increase with increasing the concentration. Besides that, Justicia adhatoda (2g/50g seeds) and Artemisia dubia (1g/50g seeds) were also found as good candidates to use for the control of Sitophilus zeamais. Qualitative tests done for the presence of various phytochemicals showed that Azadirachta indica Juss and Artemisia dubia Wall. were found to contain most of the tested metabolites among all botanicals. Most of those phytochemicals were extracted by the aqueous solution rather than other 3 menstrums used (acetone, methanol and petroleum ether). Similarly, quantitative test revealed that percentage of alkaloid was found 18.01%, 17.78%, 17.75%, 11.58%, 8.20%, 6.78% and flavonoid15.32%, 13.63%, 12.86%, 11.36%, 7. 51% 2.85% respectively in L. camara L. P. hysterophorus L., J. adhatoda Linn., M. oleifera L., A. indica Juss. and A. dubia Wall. Leaf extract of L. camera found to contain highest botanicals extract reach in alkaloid and flavonoid mayhave insecticidal property and could be use as grain protectant percentage of alkaloid (18.01%) as well as flavonoid (15.32%) So, it was concluded that the locally available against the maize weevil at storage condition in cost effective and eco-friendly manner; however, application at proper dose is necessary to maintain the efficacy at higher level.

scholarly journals Bio-efficacy of Clerodendrum capitatum (Willd) Schumachet. Thonn. (Lamiales: Verbenaceae) against Dermestes maculatus De Geer, 1774 (Coleoptera: Dermestidae) larvae infestation on smoked catfish Claria gariepinus (Burchell, 1822) (Siluriformes: Clariidae)

2016 ◽  
Vol 3 (5) ◽  
pp. 37 ◽  
Author(s):  
Jacobs Mobolade Adesina ◽  
Adeolu Rufus Jose ◽  
Yallappa Rajashekar
Keyword(s):  
Weight Loss ◽  
Larval Mortality ◽  
Management Strategies ◽  
Clarias Gariepinus ◽  
Adult Emergence ◽  
Harvest Management ◽  
Plastic Container ◽  
Dermestes Maculatus ◽  
Fish Samples ◽  
Leaf Powder

Studies were conducted under ambient condition to assess the efficacy of Clerodendrum capitatum (Willd) Schumachet. Thonn. (Lamiales: Verbenaceae) against larvae infestation and adult emergence of Dermestes maculatus De Geer, 1774 (Coleoptera: Dermestidae) on smoked dried catfish Claria gariepinus (Burchell, 1822) (Siluriformes: Clariidae). The leaf powder was admixed at 2.5 g, 3.0 g, 4.0 g and 5.0 g/15 g to disinfected smoke-dried catfish Clarias gariepinus separately in a 125 mL plastic container. Ten newly emerged (0-72 h) larvae of D. maculatus were introduced into each of the plastic containers. Efficacy of Clerodendrum capitatum was assessed based on percentage larval mortality, adult emergence reduction and weight loss. Results showed that the plant powder cause significantly (p < 0.05) larval mortality compared to the untreated fish at 24 h, 48 h and 72 h post infestation. Percentage reduction of adult emergence was not significant (p > 0.05) in treated fish samples compared to untreated sample. While fish sample protected with powder of Clerodendrum capitatum, exhibit significant (p < 0.05) low weight loss as against weight loss observed in untreated fish. The efficacy of Clerodendrum capitatum is inversely proportional to increase in dosage rate. This study clearly demonstrated the larvicidal potential of Clerodendrum capitatum powder in offering effective protection against infestation and damage by D. maculatus and recommended for utilization in integrated post-harvest management strategies of smoke-dried fish.

scholarly journals PENGARUH EKSTRAK DAUN KERSEN (Muntingia calabura) DENGAN DOSIS BERBEDA TERHADAP LAMA INKUBASI, DAYA TETAS DAN KELULUSHIDUPAN LARVA IKAN LELE DUMBO (Clarias gariepinus)

2020 ◽  
Vol 36 (1) ◽  
pp. 99-110
Author(s):  
Harni Sri Mulyani ◽  
T. Iskandar Johan
Keyword(s):  
Water Quality ◽  
Experimental Method ◽  
Incubation Time ◽  
Leaf Extract ◽  
Clarias Gariepinus ◽  
Optimal Dose ◽  
Quality Parameters ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Muntingia Calabura

ABSTRACT This study aims to determine the effect of the optimal dose of cherry leaf extract solution on incubation time, hatchability, and survival of larvae of catfish (Clarias gariepinus). The research used an experimental method using a Completely Randomized Design (CRD) with 5 treatments and 3 replications, namely: (P1) 2,0 gr/l, (P2) 2,5 gr/l, (P3) 3,0 gr/l , (P4) 3,5 gr/l and (P5) 4,0 gr/l. The test eggs used came from artificial spawning at the Fish Seed Center (BBI), Riau Islamic University. The container used is a jar with a capacity of 10 liters of 15 pieces. This research was conducted for 21 days on October 2019 at the Laboratory of Fish Seed Center (BBI), Faculty of Agriculture, Riau Islamic University, Pekanbaru. The results showed that the best adhesion was found on (P5) 4,0 gr/l of 98,89%. The incubation time and hatchability of the best treatment at (P3) 3,0 gr / l, the incubation time is 17 hours 27 minutes with a hatchability of 83,33%. Furthermore, the best treatment life was on (P2) 2,5 gr/l was 42,08%. The results of measurements of water quality parameters in this study had temperatures ranging from 290 C - 340 C, pH 6,0-6,5, ammonia between 0,28-0,97 and DO range between 3,14-3,81. Keywords:   Dumbo catfish, Long incubation, Hair power, Graduation, Cherry leaves, Hatchability

scholarly journals EFFECTIVENESS OF CHERRY LEAF EXTRACT (Muntingia calabura) WITH DIFFERENT LEVELS AS TREATMENT OF SEEDS OF SANGKURIANG CATFISH (Clarias gariepinus) INFECTED BY Trichodina sp

2018 ◽  
Vol 1 (1) ◽  
pp. 41
Author(s):  
Moh. Nurhuda ◽  
Marry Ann Kholista ◽  
Yuliani Ismi ◽  
Nurfitri Maulidiya ◽  
Hariyadi Hariyadi ◽  
...  
Keyword(s):  
Human Body ◽  
Leaf Extract ◽  
Clinical Symptoms ◽  
Clarias Gariepinus ◽  
Outer Part ◽  
Quality Measurement ◽  
The Body ◽  
Triterpenoid Saponin ◽  
Randomized Design ◽  
Muntingia Calabura

Sangkuriang catfish (Clarias gariepinus) is a leading commodity of Indonesian freshwater fishery. Production continues to increase in 2016 reached 22%. cultivators experience constraints on seeding and enlargement due to ectoparasites Trichodina sp. this parasite attacks the outer part of the body marked by the spots on the body. Prevention and treatment of many usses of chemicals that can adversely affect the human body and environtment, so it takes another alternative to replace these chemicals that can adversely affect the human body and environment, so it takes another alternative to replace these chemicals. Cherry Leaf contain compounds Flavonoid, Tannin, Triterpenoid, Saponin and Polifenol as antioxidants, Cherry Leaf useful as an antibacterial. The method used in this research is the experimental method and using Completely Randomized Design (RAL). Concentrations of cherry leaf extract (Muntingia calabura) given include 1%, 2%, 3%, 4% and 5% there were 5 treatments and 4 replications. The data obtained were analyzed by ANAVA method (variance analysis) followed by LSD test. The results showed that the use of cherry leaf extract (Muntingia calabura) in treating Trichodina sp parasite has a very real effect with the best value on the presentation of Leukocrit is 2% concentration that produces Leukocrit percentage of 2.68% and percentage of Hematocrit 2% with Hematocrit yield of 11.83%. The clinical symptoms are white spots in all body, pale color, decreased appetite of the mucus that comes out, often rubbing the body to the wall. The result of water quality measurement shows temperature: 24 - 27,2oC, DO: 4,1 - 6,9 and pH: 6 - 8.KEY WORDS: Muntingia calabura, Trichodiniasis, Sangkuriang Catfish, Leukocrit, Hematocrit.

scholarly journals Comparison of Polyaromatic Hydrocarbon Residue Concentrations in Clarias gariepinus Smoked with Traditional and Mechanical Kilns

2020 ◽  
Vol 10 (28) ◽  
Author(s):  
Oluseun Osineye ◽  
Ayo Jesutomi Abiodun-Solanke ◽  
Elizabeth Mangai ◽  
Ebele Okeke ◽  
Bethel Jahnezim
Keyword(s):  
Health Hazard ◽  
Clarias Gariepinus ◽  
Smoked Fish ◽  
Randomized Design ◽  
Fish Samples ◽  
Porcelain Mortar ◽  
Polycyclic Aromatic ◽  
Financial Interests ◽  
Average Size ◽  
Human Health Hazard

Background. Wood, a common fueling material for fish smoking in Nigeria, contains polycyclic aromatic hydrocarbons (PAHs) which have been found to be carcinogenic and pose a human health hazard. Objectives. The present study investigated the interactions and effects of kiln types on levels of PAHs in smoked fish. Methods. Twenty fresh samples of Clarias gariepinus with an average size of 800 g were eviscerated, washed and salted for smoking. Sixteen (16) of the fish were randomly and equally allotted to each of the two kilns (treatments) and replicated three times in a completely randomized design. Each kiln was loaded and heated up with hard wood charcoal and the effects were evaluated on the resulting smoked fish. The remaining four fish samples were kept under refrigeration (controls). Samples of the smoke-dried fish from each kiln were homogenized using a porcelain mortar and pestle, sieved through a 250-μm2 sieve, and packaged in labeled airtight containers prior to extraction. Pure extracts from the samples were subjected to gas chromatography. Results. The results showed that the PAH concentration in non-smoked catfish was 1.0 mg/kg, the PAH concentration in fish samples smoked with a traditional kiln was 2.0 mg/kg, and no PAHs were detected in samples smoked with a mechanical kiln. Conclusions. The level of PAH contamination of smoked fish is dependent on the type of kiln used, as demonstrated by the differences between fished smoked with traditional and mechanical kilns in the present study. Competing Interests. The authors declare no competing financial interests

Potential of Five Local Plant as Protectant against Hide Beetle, Dermestes maculatus Degeer Infesting Dry Cat Fish; Clarias gariepinus Burchell

2018 ◽  
pp. 1-8
Author(s):  
A. Jatau ◽  
Q. Majeed ◽  
M. A. Yahaya ◽  
M. B. Sokoto
Keyword(s):  
Weight Loss ◽  
Clarias Gariepinus ◽  
Post Treatment ◽  
Ambient Conditions ◽  
Piper Guineense ◽  
Dermestes Maculatus ◽  
Randomized Design ◽  
Plant Powders ◽  
Local Plant ◽  
Dried Fish

Efficacy of varying amounts of five selected local plant namely : Balanite aegyptiaca, Eugenia aromatica, Piper guineense, Ocimum gratisimum, and Ziziphus mauratania against the mortality, oviposition, progeny emergence and  weight loss of dried fish due to infestation by  Dermestes maculatus was studied under ambient conditions. The plant were pulverised into powders and applied as 2.0, 4.0 and 6.0 g per 50 g of dried fish. Three pairs of newly emerged adults by  were inoculated into each of the treated fish in a beaker, arranged in a Complete Randomized Design  in the laboratory. The result showed all the plant powders tested evoked significant mortalities of adults at the highest concentration (6) after 3 and 7 days of post-treatment when compared with untreated control. The rate of oviposition was drastically suppressed by all plant powders, especially at higher concentration.  The number of adults emerged were significantly lower than control at (p>0.05).  Similarly, all the plant's powders significantly prevented weight loss at highest concentration after 12 weeks of post-treatment periods. These results have shown that tested plants demonstrated potential ability against adults hide beetle infesting dried fish.

scholarly journals Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Distilled Water ◽  
Treatment Groups ◽  
Randomized Design ◽  
Neem Leaves ◽  
Neem Leaf Extract

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 

Piper guineense: A potent ethnobotanical preservative against Dermestes maculatus in smoke-dried African catfish, Clarias gariepinus

2021 ◽  
Vol 16 (2) ◽  
pp. 1-6
Author(s):  
Omosowone Olajumoke
Keyword(s):  
Clarias Gariepinus ◽  
Fine Powder ◽  
Nutritional Composition ◽  
African Catfish ◽  
Chemical Compositions ◽  
Plastic Container ◽  
Piper Guineense ◽  
Dermestes Maculatus ◽  
Fish Samples ◽  
Dried Fish

Piper guineense fruits were purchased, sun-dried (30±2oC) for 10 days and ground into fine powder. Fifteen (15) pieces of Clarias gariepinus (300 ± 5.0 g) were procured and smoke-dried in the laboratory using a charcoal-powered smoking kiln at 65oC for 10 hours. Each smoke-dried fish sample was placed in a plastic container with perforated lids covered with muslin cloth and Piper guineense fruit meal was sprinkled at varying quantities (0.0 g, 2.5 g, 5.0 g, 7.5 g and 10.0 g). Ten (10) adults of Dermestes maculatus bred from highly infested smoke-dried fish samples were introduced into each container and covered air-tight for seven (7) days. Mortality of D. maculatus was significantly different (p = .05) in all treatments and increased with an increase in the quantity of P. guineense and number of days. Mortality ranged between 73.33 and 100 % in treated groups while none was recorded in the control. Chemical compositions in treatments showed an increase in crude protein, lipids and lower moisture content at higher inclusion levels. This study showed that P. guineense can prolong the shelf-life of smoke-dried C. gariepinus against D. maculatus while retaining its nutritional composition. Key words: Dermestes maculatus, Piper guineense, smoke-dried, Clarias gariepinus

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