scholarly journals Conditioning in the promotion and uniformization of Umbu seed germination

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
Renato Teo Barros ◽  
Cibele Chalita Martins ◽  
Francisco Elder Carlos Bezerra Pereira ◽  
Givanildo Zildo da Silva
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Water Content ◽  
Environmental Conditions ◽  
Seed Quality ◽  
Germination Rate ◽  
Ambient Conditions ◽  
Germination Test ◽  
Mean Germination Time ◽  
Cold Chamber

Abstract Seed dormancy may decrease during storage and some environmental conditions may accelerate this process. The aim of this work was to determine efficient techniques to condition umbu seeds in order to promote and standardize their germination. Seeds were stored for 180 days in paper bags kept in five ambient conditions: laboratory (25 ºC and 55% RH); warm oven (40 °C and 53% RH); hot oven (50 °C and 49% RH); dry chamber (18 °C and 65% RH) and cold chamber (10 °C and 65% RH). Seed quality was evaluated every 60 days by means of the following tests and determinations: water content; germination test (25 °C and 55% RH, weekly evaluated up to 91 days after sowing); first count test (14 days); germination rate index; mean germination time and electrical conductivity. The conditioning of umbu seeds in laboratory, or in warm oven (40 ºC) used efficient techniques to promote and standardize germination; under these conditions, after six months of storage, germination increased from 31% to 84 and 74%, respectively.

scholarly journals Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain

Botany ◽  
2012 ◽  
Vol 90 (8) ◽  
pp. 731-742 ◽  
Author(s):  
Félix Pérez-García ◽  
Federico Varela ◽  
M. Elena González-Benito
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Germination Rate ◽  
Seed Mass ◽  
Germination Percentage ◽  
Gentiana Lutea ◽  
Moist Chilling ◽  
Mean Germination Time ◽  
Wild Accessions ◽  
Seed Water Content

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.

scholarly journals Deleterious Effects of Storage Environmental Conditions on The Seed Quality of Two Varieties of Tomato (Solanum lycopersicum L.) Stored in Triple Laminated Aluminum Packing in Sri Lanka

2020 ◽  
Vol 8 (4) ◽  
pp. 437-447
Author(s):  
R.A.I.S. Ariyarathna ◽  
S. L. Weerasena ◽  
C. K. Beneragama
Keyword(s):  
Sri Lanka ◽  
Seed Germination ◽  
Environmental Conditions ◽  
Seed Quality ◽  
Quality Parameters ◽  
Vegetable Crops ◽  
Ambient Conditions ◽  
Tomato Seeds ◽  
Vigour Index ◽  
Field Emergence

Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.  

scholarly journals Impacts of different salt source and concentrations on germination and seedling growth of many pumpkin seeds used as rootstoch in Iran

Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Germination Rate ◽  
Shoot Length ◽  
Root Weight ◽  
Pumpkin Seeds ◽  
High Concentrations ◽  
Shoot Weight

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.

scholarly journals ELECTRICAL CONDUCTIVITY AND QUALITY OF DIFFERENT UMBEL ORDER CARROT SEEDS

2020 ◽  
Vol 9 (04) ◽  
pp. 75-81
Author(s):  
Luana de Souza Marinke ◽  
Hugo César Rodrigues Moreira Catão ◽  
Gabrielly Fernanda Francisco ◽  
Ítala Menegon Castilho ◽  
Letícia Rodrigues de Oliveira ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Germination Rate ◽  
Statistical Design ◽  
Germination Test ◽  
Aerial Portion ◽  
Initial Characterization ◽  
Different Temperatures ◽  
Physiological Quality ◽  
Final Score

Carrot seeds exhibit irregular vigor due to the wide blooming period of the crop. Results from germination tests are insufficient to evaluate the physiological potential. Thus, vigor tests are used in combination with germination tests. The objective of this work was to evaluate the quality of carrot seeds from different umbel order and the electrical conductivity under different temperatures and imbibing periods. Carrot seeds from cultivar Brasilia were used to perform the experiments. Content of water, the first and the final score of the germination test, index of germination rate, and length of roots and of the aerial portion were determined in the initial characterization. The electrical conductivity test was completed with replicates containing 50 seeds, in 25 mL deionized water at 20, 25 and 30oC. Evaluations were performed with 2, 4, 6, 8, 12 and 24 hours of imbibing period. The experiment was accomplished under a completely random statistical design. The quality of carrot seeds varied according to the umbel order. Seeds of secondary umbels showed better physiological quality. The electrical conductivity test is efficient to evaluate the vigor of carrot seeds with 8 hours of imbibing period at 25°C.

scholarly journals EFFECTS OF TEMPERATURE, SUBSTRATE AND LUMINOSITY CONDITIONS ON CHIA SEED GERMINATION

2019 ◽  
Vol 32 (2) ◽  
pp. 411-418
Author(s):  
CLAUDIA KELY PIRES DE MATTOS SORANA ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
FERNANDA BRITO CARDOSO ◽  
TIAGO ROQUE BENETOLI DA SILVA ◽  
ANA CARINA DA SILVA CÂNDIDO ◽  
...  
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Germination Test ◽  
Paper Substrate ◽  
Randomized Design ◽  
Chia Seed ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Effects Of Temperature ◽  
Completely Randomized Design

ABSTRACT The aim of the present study was to evaluate the effects of temperature, substrate, and luminosity on chia seeds in order to determine adequate conditions for a germination test. The experiment consisted of a completely randomized design, in a 4 × 5 × 2 factorial scheme (four substrates × five temperatures × two luminosity conditions), with four replications. The following variables were analyzed: first germination count, germination, germination speed index, and mean germination time. Germination was best at 15, 20, 25, and 30 °C for the on-paper, paper roll, and sand substrates. However, in the presence of luminosity and at 25 °C, the on-paper substrate had the highest mean. For all substrates, the lowest germination was achieved at 35 °C, proving that high temperatures reduce chia seed germination. Germination speed index was highest at 25 and 30 °C for on-paper, paper roll, and sand substrates, in both the presence and absence of luminosity. Chia seeds are considered to be neutral photoblasts. Germination tests should be performed with the on-paper substrate, at 25 °C, and with a duration of seven days. The first count should be carried out four days after establishment of the test.

scholarly journals Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

2019 ◽  
Vol 11 (7) ◽  
pp. 42
Author(s):  
Luciana Maria da Silva ◽  
Anderson Dias Vaz de Souza ◽  
Rogério Lámim Silva Junior ◽  
Natália Arruda ◽  
Nei Peixoto ◽  
...  
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Precise Determination ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Seedling Length ◽  
Aging Test ◽  
Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

scholarly journals Effect of Seed Treatments and Containers on Chilli and Brinjal Seed Viability

2019 ◽  
pp. 1-9
Author(s):  
Sunil Kumar ◽  
Suryapal Singh ◽  
S. S. Jakhar
Keyword(s):  
Electrical Conductivity ◽  
Seed Treatment ◽  
Seed Quality ◽  
Seed Viability ◽  
Quality Parameters ◽  
Packaging Material ◽  
Packaging Materials ◽  
Ambient Conditions ◽  
Pathology Laboratory ◽  
Seed Pathology

An experiment was conducted to investigate the influence of fungicides and packaging materials on longevity of chilli (variety: RCH-1) and brinjal (variety: Hisar Shyamal) seeds. The seeds were treated with 15 fungicides and were kept in three containers viz. Metal box, Cloth bag and Plastic zipling bag up to 12 months under ambient conditions in seed pathology laboratory of Department of Seed Science & Technology, CCSHAU, Hisar, India. The samples were drawn at quarterly intervals for ascertaining the seed quality parameters. The seeds treated with flusilazole and carbendazim (2 g kg-1 seed) in case of chilli and brinjal and stored in metal-box were found better for maintenance of higher seed quality parameters [electrical conductivity and dehydrogenase activity] during the study period. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of chilli and brinjal seeds.

scholarly journals Germination and seed conservation of a pioneer species, Tecoma stans (Bignoniaceae), from tropical dry forest of Colombia

2018 ◽  
Vol 66 (2) ◽  
pp. 918
Author(s):  
Jhon Alexander Vargas-Figueroa ◽  
Alba Marina Torres-González
Keyword(s):  
Seed Germination ◽  
White Light ◽  
Germination Rate ◽  
Red Light ◽  
Tropical Dry Forest ◽  
Seed Storage ◽  
Dry Forest ◽  
Storage Behavior ◽  
Mean Germination Time ◽  
Seed Storage Behavior

Seed germination and seed longevity under different environmental conditions are fundamental to understand the ecological dynamics of a species, since they are decisive for its success within the ecosystem. Taking this into account, seed germination and seed storage behavior of a pioneer species of tropical dry forest (Tecoma stans) were studied in the laboratory, to establish the effect of different environmental conditions on a local tree population. Two seed lots collected in July 2011, from Cali (Colombia), were evaluated under three alternating temperatures (20/30, 20/25, 25/30 ºC; 16/8 h) and four light qualities (12-hour white light photoperiod, darkness, and 15 minutes of red light -R and far red light -FR). Final germination was recorded for all treatments; for white light treatment the daily germination was recorded to calculate mean germination rate, mean germination time, and two synchronization indices. To assess the effect of light quality on physiological variables, a destructive germination test was carried out. For this test, another seed lot was evaluated under the same light conditions using an alternating temperature of 20/30 °C - 16/8 h, recording germination during six days for every treatment. In addition, seeds were stored at two different moisture contents (7.7, 4.1 %) and three storage temperatures (20, 5, -20 ºC), during two time periods (one and three months); a seed germination test was conducted for each treatment. Four replicates of 35 seeds per treatment were used for all experiments. Germination was high (GP > 90 %) with all alternating temperatures under white light, whereas under R, FR, and darkness germination was evenly successful at low temperatures, but at higher temperature, half of the seeds entered into secondary dormancy (GP= 45-65 %). However, mean germination rate and synchronization under R and FR decreased significantly in comparison to white light treatment and, consequently, mean germination time increased. Seed storage behavior of this species is orthodox due to the high germination (GP > 90 %) obtained under all treatments. In conclusion, T. stans seeds have a negative germination response at high incubation temperatures in the absence of white light, entering into a secondary dormancy. In contrast, an environment with a lower temperature and without white light delays the germination, but at the end seeds are able to reach the same germination values. This seed dependence on incident light in limiting conditions suggests a physiological mechanism on the seed tissues of this species, probably mediated by phytochromes. Finally, the orthodox seed storage behavior of T. stans is a reason to include this species in ex situ seed conservation programs for restoration and recovery of the tropical dry forest; however, long-term studies should be conducted in order to evaluate the maintenance of this characteristic throughout longer periods of time. Rev. Biol. Trop. 66(2): 918-936. Epub 2018 June 01. 

scholarly journals Improving the method for determining the physiological and sanitary potential of Gherkin seeds

2021 ◽  
Vol 37 ◽  
pp. e37087
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Ítala Menegon Castilho ◽  
Franciele Caixeta ◽  
Nilvanira Donizete Tebaldi ◽  
Pâmela Gomes Nakada Freitas
Keyword(s):  
Water Content ◽  
Saline Solution ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Analysis Method ◽  
Germination Test ◽  
Seed Technology ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.

scholarly journals A new framework for modelling seed germination and seedling tillering of winter wheat in the field

2021 ◽  
Author(s):  
Jinping Chen ◽  
Peter Whalley ◽  
Yang Gao ◽  
xiaoxian zhang ◽  
Malcolm J. Hawkesford ◽  
...  
Keyword(s):  
Seed Germination ◽  
Winter Wheat ◽  
Water Content ◽  
Soil Water ◽  
Soil Water Content ◽  
Genetic Heterogeneity ◽  
Germination Rate ◽  
Environmental Cues ◽  
Metabolic Reactions ◽  
New Framework

Seed germination is regulated by multiple environmental cues and understanding their relationships is critical to planning seed drilling and subsequent seedling management. We develop a new framework by viewing the metabolic reactions associated with seed germination as a moving event in a physiological dimension to simulate seed germination. Fluctuations in environmental cues and genetic heterogeneity of seed lot make the metabolic reactions in each seed uncertain, and we use an average germination rate to describe the average metabolic reactions and a dispersion coefficient to describe the genetic heterogeneity. We apply the model to winter wheat seeds drilled at different dates in plots under different soil water contents and prove that the model accurately reproduces the time course of germination in all treatments. We found the average germination rate increases nonlinearly with temperature in the base-suboptimal temperature range, and there is an optimal soil water content where the germination rate peaks due to soil anaerobicity. Our model can be fitted to field data using temperature and soil water content to describe the trade-off impact of soil water on soil anaerobicity and imbibition, whereas the difficulty of obtaining accurate water potential and oxygen measurements makes this difficult with the hydrothermal time models.

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