scholarly journals Morphology of endothelial cells from different regions of the equine cornea

2016 ◽  
Vol 46 (12) ◽  
pp. 2223-2228 ◽  
Author(s):  
Cláudia Skilhan Faganello ◽  
Vanessa Ruiz Moura da Silva ◽  
Maria Cristina Caldart de Andrade ◽  
André Silva Carissimi ◽  
João Antonio Tadeu Pigatto
Keyword(s):  
Endothelial Cells ◽  
Optical Microscopy ◽  
Morphological Analysis ◽  
Statistical Data ◽  
Corneal Endothelial Cells ◽  
Statistical Data Analysis ◽  
Alizarin Red ◽  
Morphological Differences ◽  
Student’S T ◽  
Student’S T Test

ABSTRACT: The objective of this study was to evaluate the morphology of different regions of the equine cornea using optical microscopy. Both healthy eyes of eight horses, male or female, of different ages were evaluated. Corneas were stained with alizarin red vital dye and subsequently examined and photographed using optical microscopy. Corneal endothelial morphology of central, superior, inferior, temporal and nasal areas was assessed. One hundred endothelial cells from each corneal area were analyzed. The shape of the corneal endothelial cells of each corneal region was analyzed. Statistical data analysis was conducted using the Student's t test. Values of P<0.01 were considered significant. Regarding morphological analysis, no statistically significant differences were reported between the equine corneal regions analyzed. The present research suggested that there are no morphological differences between regions of the equine cornea. The values obtained in any region of the equine cornea can be extrapolated to other regions of the cornea and are representative of the cell morphology present in all regions of the cornea.

scholarly journals Sexual dimorphism of sharks from the amazonian equatorial coast

2015 ◽  
Vol 20 (3) ◽  
pp. 297 ◽  
Author(s):  
Jorge Luiz Silva Nunes ◽  
Ana Paula Barbosa Martins ◽  
Ednaldo Da Silva Filho ◽  
Leonardo Manir Feitosa ◽  
Luiz Phelipe Nunes e Silva ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Discriminant Analysis ◽  
Mating Behavior ◽  
T Test ◽  
Internal Fertilization ◽  
Morphometric Characters ◽  
Morphological Differences ◽  
Student’S T ◽  
Different Levels ◽  
Student’S T Test

Sexual dimorphism is a widespread feature in several groups of vertebrates. Chondrichthyans differ sexually due to the presence of the clasper, a structure for internal fertilization, and other sexual differences in secondary characteristics. Nevertheless, studies assessing these variations are fairly rare. The main goal of this study is to identify differences in sexual dimorphism in three species of sharks from the Carcharhinidae family (<em>Rhizoprionodon porosus</em>, <em>Carcharhinus porosus</em> and <em>Isogomphodon oxyrhynchus</em>) using morphometric tools. A total of 213 specimens were captured in the Amazonian Equatorial Coast and analyzed using 65 morphometric characters. Discriminant analysis and The Student's t-test were used to demonstrate the morphological differences among sexes. Sexual dimorphism was reported at different levels for the three species. This study suggests that the most likely explanation for the presence of these variations is related to their reproductive characteristics and mating behavior.

scholarly journals Morphology of Endothelial Cells from Different Regions of the Swine Cornea

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Lídia Luzia Clerot ◽  
Paula Stieven Hünning ◽  
Marcelle Bettio ◽  
Marcela Torikachvili ◽  
Michelle Beck Petersen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Optical Microscopy ◽  
Repeated Measures ◽  
Corneal Endothelium ◽  
Anterior Segment ◽  
Optical Microscope ◽  
Specular Microscopy ◽  
Microscope Slide ◽  
Large White ◽  
Alizarin Red

Background: The corneal endothelium is a monolayer of polygonal cells which constitute the last layer of the cornea. The integrity of this layer is critical to cornea transparency. The characterization of normal corneal endothelial morphology is important not only to clinical evaluation but also to selection of areas of the cornea with better quality to be employed as donor tissue. The aim of the present study was to evaluate the morphology of endothelial cells from different regions of the swine cornea after alizarin red staining using optical microscopy.Materials, Methods & Results: Twenty-four healthy eyes from 12 swine Large White breed, with 14-month-old, males or females obtained from a licensed Brazilian commercial slaughterhouse were studied. Immediately after humane slaughter, the eyes were enucleated and submitted to ophthalmic examination. Eyes with signs of diseases of the anterior segment were excluded. The cornea, with 3 mm of the sclera, was removed and placed on a glass microscope slide with the endothelial side up. Four radial incisions were made in the periphery of the cornea to better accommodate the cornea on the microscope slide. Alizarin red was diluted in isotonic solution (0.2 g/100 mL) and the pH was adjusted to 4.2 with hydrochloric acid. Three drops of alizarin red were placed on the corneal endothelium. After 90 s, the dye was removed from the cornea with balanced saline solution. The corneal endothelium was examined and photographed using an optical microscope. All evaluations were performed by the same investigator. Photomicrographs were taken of central, superior, inferior, nasal and temporal corneal areas. Parameter studied included endothelial cell morphology. For the statistical analysis, was employed the ANOVA variance test (repeated measures). Differences were considered statistically significant at P < 0.05. Normal endothelium cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. There were no significant statistical differences in the proportion of the morphology and the different regions of the cornea (P = 0.31).Discussion: Different techniques are available for the analysis of corneal endothelium, including mainly scanning electron microscopy, specular microscopy and optical microscopy. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and to obtain images of the swine endothelium of all regions of the cornea.  The analysis of the cellular morphology or the percentage of hexagonal cells are among the main parameters used to evaluate the health of the corneal endothelium. In this study, the endothelium had the predominance of the hexagonal shape in all regions studied. In swine, there are no studies evaluating the shape of the endothelial cells in the five different regions of the cornea. This study has demonstrated that the parameters evaluated in swine did not differ significantly between the various places of the cornea.

scholarly journals A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells

2021 ◽  
Vol 29 ◽  
pp. 531-538
Author(s):  
Xueyang Zhang ◽  
Yanyi Liu ◽  
Yuan Su ◽  
Xiaohui Fan ◽  
Fei Hu
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Mrna Level ◽  
Cell Counting ◽  
Control Group ◽  
Real Time Quantitative Pcr ◽  
Student’S T ◽  
Umbilical Vein Endothelial Cells ◽  
Student’S T Test

OBJECTIVE: We aimed to investigate the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system and cell proliferation of umbilical vein endothelial cells. METHODS: Human umbilical vein endothelial cells (HUVECs) were used in this research. There are two induvial groups, control group and hydroxyapatite bioceramics extract treatment group. Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation. Western blot and real time quantitative PCR (Q-PCR) were used to evaluate the protein and mRNA expression levels of Ang1, Ang2 and Tie2 in Ang/Tie2 system, respectively. All the results were statistically analyzed by Spss19.0. All data were presented as mean ± standard error of mean (SEM). Student’s t-test was performed to determine the differences among grouped data. RESULTS: Hydroxyapatite bioceramics extract showed no effect on the cell morphology and cell proliferation of HUVECs. Interestingly, we found that both Ang2 and Tie2 protein and mRNA level were markedly increased by hydroxyapatite bioceramics extract. CONCLUSIONS: Hydroxyapatite bioceramic extract showed no cytotoxicity to HUVECs, and might regulate vascular remodeling by mediating Ang/Tie2 system.

scholarly journals THE ECONOMIC ASPECT OF THE APPLICATION OF SCHOLARSHIPS FOR TEACHERS AND CRITERIA FOR SCHOLARSHIPS FOR PARTICIPATION IN EDUCATION IN MEDICAL SPECIALTIES

2021 ◽  
Vol 10 (34) ◽  
Author(s):  
S.S GRECHIKHIN ◽  
Keyword(s):  
Statistical Data ◽  
Economic Aspect ◽  
Full Time ◽  
Medical Specialties ◽  
Effective Strategies ◽  
Economic Significance ◽  
Student’S T ◽  
Motivational Resources ◽  
Student’S T Test

Motivation plays an important role for each person. To improve the quality of students ' education, it is necessary to constantly use motivational resources. Our review is devoted to the study of the economic significance of the use of scholarships for teachers, as well as the study of the criteria for scholarships to participate in education in medical specialties. Statistical data processing included definitions of the Student's t-test and Fischer's F-test. The economic aspect included the study of the main and segmental samples. The study examined the profitability and significance of the use of scholarships and criteria for improving the quality of educational activities by scholarship teachers. In addition, our study examines the use of scholarships for career advancement and professional development in the field of professional medical education. The study will discuss potential economic obstacles and challenges in introducing possible models that will help teachers in their efforts to advance their career, improve the quality of education, and integrate income and spending on teaching. Thus, the study of the economic aspect within the framework of the profitability of full-time teachers in the field of education is exactly the area that contributes to the development of effective strategies.

scholarly journals Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

2008 ◽  
Vol 28 (9) ◽  
pp. 427-430 ◽  
Author(s):  
João A.T. Pigatto ◽  
Cristine Cerva ◽  
Cesar D. Freire ◽  
Fernando C. Abib ◽  
Luciano P. Bellini ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelium ◽  
Morphological Analysis ◽  
Specular Microscopy ◽  
Human Cornea ◽  
Corneal Endothelial Cells ◽  
Peripheral Cornea ◽  
Specular Microscope ◽  
Healthy Eyes ◽  
Number Of Cells

Both healthy eyes of 10 six-year-old male and female mongrel dogs were studied. With a contact specular microscope the corneal endothelium was examined. Endothelial cells were analyzed in the central and peripheral cornea. Morphological analysis with regard to polymegathism and pleomorphism was performed. Three images of each region with at least 100 cells were obtained. The analysis showed that polygonal cells formed a mosaic-like pattern uniform in size and shape. The predominant number of cells was hexagonal. The polymegathism index was 0.22. The study demonstrates that the morphology of the normal corneal endothelial cells of dogs is similar to that found in the human cornea.

CT-Based Morphological Analysis of The Distal Femur In Patients With Trochlear Dysplasia

2021 ◽  
Author(s):  
Weifeng Li ◽  
Qian Wang ◽  
Hui Li ◽  
Shunyi Wang
Keyword(s):  
Distal Femur ◽  
Trochlear Dysplasia ◽  
Morphological Characteristics ◽  
Control Group ◽  
Study Group ◽  
Qualitative Variables ◽  
Morphological Differences ◽  
Student’S T ◽  
Underlying Pathology ◽  
Student’S T Test

Abstract Background: The morphology of the femoral trochlear has been well investigated; however, to date, the distal femur morphological characteristics have not been studied. The research team posited the presence of morphological differences in the distal femur in trochlear dysplasia compared to a control group. They also postulated that the underlying pathology of distal femur dysplasia is a medial hypoplasia.Methods: Computed tomography (CT) scans of 80 knees with trochlear dysplasia and 80 knees with normal anatomy of the femoral trochlear were included. The following measurements were assessed by three observers: the distal femur angle, the percentage of medial distal femur height and the percentage of lateral distal femur height between the groups. The independent Student's t-test were used to evaluate the differences of the qualitative variables.Results: The distal femur angle was relatively larger compared to the control group (p<0.001). Percentage of the medial distal femur showed signifcant lower values in the study group (p<0.001). No signifcant diferences were evaluable for the percentage of lateral distal femur height (p=0.087). The study group have a bigger distal femur angle and a steep medial distal femur than the control group. Conclusion: Patients with trochlear dysplasia have a different distal femur compared with those without trochlear dysplasia. Compared to controls, patients with a dysplastic trochlea have a distal femur dysplasia, and the underlying pathology of a dysplastic distal femur is led by a medial distal femur hypoplasia.

scholarly journals Tourism Economy. Mountain Tourism: Quantitative Analysis of Winter Destinations in Romania

ECONOMICS ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 143-159
Author(s):  
Ioan-Bogdan Bacoş ◽  
Manuela Rozalia Gabor
Keyword(s):  
Quantitative Analysis ◽  
Statistical Data ◽  
Chi Square ◽  
Paired Samples ◽  
Strong Point ◽  
Chi Square Test ◽  
Ski Areas ◽  
Student’S T ◽  
Tourism Economy ◽  
Student’S T Test

Abstract The continuous development of mountain tourism derives from the multiple positive points that the mountain has in satisfying the particularly diverse tourist needs. The strong point is represented by the extremely valuable and complex tourist potential, as well as the variety of natural and anthropic resources. The complementarity of these resources makes the arrangement and development of winter resorts with a useful and attractive profile called ski areas to be more and more common in mountainous areas. The purpose of this paper is to analyze winter destinations in order to conclude whether Romania can create a competitive advantage based on this type of tourism, both at European and global level. We used statistical data for all Romanian resort for sky destination, respectively the total number of practicable kilometers, total number of cableway installations and the accommodation capacity. For the quantitative analysis we used the SPSS 23.0 statistical software and applied the following statistical methods: Person correlations, chi square test and Student’s t test for paired samples. The research results, obtained with statistical methodology, support and highlight the significant differences between the Romanian mountain resorts for winter activities.

Knowledge-Based Decision Support for Diagnosis and Therapy: On the Multiple Usability of Patient Data

1989 ◽  
Vol 28 (02) ◽  
pp. 69-77 ◽  
Author(s):  
R. Haux
Keyword(s):  
Expert Systems ◽  
Statistical Data ◽  
Patient Data ◽  
Attribute Selection ◽  
Patient Specific ◽  
Statistical Data Analysis ◽  
Inference Mechanism ◽  
Diagnosis And Therapy ◽  
Knowledge Based ◽  
Clinical Registries

Abstract:Expert systems in medicine are frequently restricted to assisting the physician to derive a patient-specific diagnosis and therapy proposal. In many cases, however, there is a clinical need to use these patient data for other purposes as well. The intention of this paper is to show how and to what extent patient data in expert systems can additionally be used to create clinical registries and for statistical data analysis. At first, the pitfalls of goal-oriented mechanisms for the multiple usability of data are shown by means of an example. Then a data acquisition and inference mechanism is proposed, which includes a procedure for controlling selection bias, the so-called knowledge-based attribute selection. The functional view and the architectural view of expert systems suitable for the multiple usability of patient data is outlined in general and then by means of an application example. Finally, the ideas presented are discussed and compared with related approaches.

Market of Insurance in Russia: Major Tendencies and Problems of Development

2006 ◽  
pp. 115-127
Author(s):  
T Natkhov
Keyword(s):  
Data Analysis ◽  
Statistical Data ◽  
Statistical Data Analysis ◽  
Medium Term ◽  
Insurance Sector ◽  
Different Types ◽  
Basic Characteristics

The article considers recent tendencies in the development of the market of insurance in Russia. On the basis of statistical data analysis the most urgent problems of the insurance sector are formulated. Basic characteristics of different types of insurance are revealed, and measures on perfection of the insurance institution in the medium term are proposed.

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