scholarly journals Changes in serum adenosine deaminase and isoenzyme levels in addition to routine liver biochemical parameters in sheep with chronic fascioliasis

2022 ◽  
Vol 52 (4) ◽  
Author(s):  
Nuri Altuğ ◽  
Yıldıray Başbuğan ◽  
Nazmi Yuksek
Keyword(s):  
Bile Duct ◽  
Adenosine Deaminase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Total Bilirubin ◽  
Clinical Signs ◽  
Infected Group ◽  
Direct Bilirubin ◽  
Control Group ◽  
And Control

ABSTRACT: This study assessed changes in the levels of adenosine deaminase (ADA) and its isoenzymes in addition to routine liver biochemical parameters in sheep with fascioliasis. The study was conducted on 35 Akkaraman sheep. Of these, 25 sheep were diagnosed with fascioliasis based on anamnesis and clinical signs, and had endoparasites based on parasitological examinations (Fasciola-infected group). The remaining 10 sheep that were sampled from a single healthy herd (same flock) different from the infected group did not have any clinical signs or endoparasites (control group). Total protein (TP), albumin (ALB), and globulin (GLB) levels gradually increased on days after treatment compared to the values measured before treatment; the increases were statistically significant on all days for TP levels but only on day 14 after treatment for GLB levels (P < 0.05). Although, the ALB levels did not increase significantly on days after treatment, the ALB level and ALB/GLB ratio on days 7 and 14 after treatment were still lower than the values of day 21 after treatment and control group (P < 0.05). Total bilirubin (T-Bil) and direct bilirubin (D-Bil) levels on days 14 and 21 were significantly lower than that of day 0 (before treatment) and day 7 after treatment (P < 0.05). These results indicated that the increase in adenosine deaminase (ADA) and ADA1 levels may be due to possible concomitant infection of Fasciola larvae (in the parenchyma) and adults (in the bile duct).

scholarly journals Infection of sheep with Strongyloides papillosus: The effect of intensity of parasitic infection as well as therapy with albendazole on certain biochemical parameters in the blood of sheep

2015 ◽  
Vol 69 (1-2) ◽  
pp. 41-61
Author(s):  
Blagoje Dimitrijevic ◽  
Slavoljub Jovic ◽  
Milanka Jezdimirovic ◽  
Dragan Bacic ◽  
Mila Savic ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Total Bilirubin ◽  
Parasitic Infection ◽  
Infection Intensity ◽  
Total Activity ◽  
Control Group ◽  
Growth Trend ◽  
Calcium Phosphorus

The objective of this work was to investigate certain biochemical parameters in the blood of sheep in conditions of different intensity of parasitic infection with Strongyloides papillosus, and also after the therapy with albendazole. The research was carried out on the sheep of Wurtemberg breed (n = 30) in which there was determined low, medium and high intensity of parasitic infection with S. papillosus. The control group (n = 10) consisted of the sheep that were negative to the presence of the parasitic infection. The degree and type of changes were monitored by determining the concentration of glucose, total protein, albumin, Albumin/Globulin ratio, AST, urea, total bilirubin, calcium, phosphorus, lactate dehydrogenase (LDH) total activity and isoenzyme distribution LDH1-5. On the basis of the obtained results it was concluded, through isoenzyme distribution of lactate dehydrogenase, that parasitic infection with S. papillosus can cause damage to the liver, heart muscle and lungs, while after the therapy with albendazole, the most affected organ is the liver. The concentration of glucose, total protein and albumin linearly decreased with the increase of intensity of the parasitic infection (p < 0,05), and after the therapy with albendazole, a drop in concentration of these parameters was at a statistically significantly higher level (p< 0,01). AST activity as well as the concentration of urea and total bilirubin grew linearly along with the intensity of the parasitic infection (p < 0,05). After the therapy with ABZ, AST activity and concentration of total bilirubin were at a statistically significantly higher level (p < 0,001), while urea concentration kept the same level as in the case of the parasitic infection. The values of calcium concentration (p > 0,05) as well as phosphorus (p < 0,05) linearly decreased with the parasitic infection intensity growth. Trend towards the decline of the concentration of these macroelements continues even after the therapy with albendazole (p < 0,001). Considering our previous research in the field of oxidative stress, a phenomenon that is fundamentally related to the changes determined in this work, we strongly recommend that besides antihelmintics, preparations with antioxidative properties should be used in antiparasitic protocols as well. <br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/VETGL190125001E">10.2298/VETGL190125001E</a><u></b></font>

scholarly journals Experimental infection on the locally isolated avian infectious laryngotracheitis virus

2017 ◽  
Vol 41 (1) ◽  
pp. 1-4
Author(s):  
Zaid Haddam Taha
Keyword(s):  
Experimental Infection ◽  
Post Infection ◽  
Clinical Signs ◽  
Elisa Test ◽  
Infected Group ◽  
Control Group ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
And Control

     The aim of this study was to evaluate virulence of local isolated avian infectious laryngotracheitis virus in experimentally infected chicken. Forty chickens 10 weeks old were used for the experimental infection with the locally isolated infectious laryngotracheitis virus. Chickens were divided into three groups, the first group consisted from 20 chickens infected with isolated infectious laryngotracheitis virus (2×104.16 TCID 50/50 µl) via eyes and mouth drops (one drop for each). The second group consisted of 10 chickens (non-infected) in contact with infected group inoculated with maintenance media (Minimum essential medium) on their eyes, to observe if the infected group can spread the virus. The third group consisted from 10 chickens (non-infected) were left as a control group separated from other groups, inoculated with maintenance media (Minimum essential medium) on their eyes. Clinical signs and mortality were examined daily up to 12 days post infection. The main clinical signs were depression coughing and gasping with mild conjunctivitis and no mortality. Enzyme linked immunosorbent assay (ELISA) test was conducted on the collected sera of chickens before and after experimental infection with isolated virus. The results of ELISA test was negative for all groups of chickens before experiment  and positive results for infected group with titer approximately ranging from (2534-7910); Measure of central tendency and dispersion were used with mean (4874.75) and stander error (355.96\ 13.6%); while negative results for contact group and control group. Eighteen chickens (10 weeks old) separately were divided into three groups (infected, contact and control) treated as mention above  and were used   for histopathological examination; the chickens were killed, two in each group at 24 hr., 48 hr. and 72 hr. post infection. The histopathological changes on trachea and larynx were intracellur inclusion bodies formation detected at 72hr., post infection for infected group only.

Effects of E.coli O157:H7 Experimental Infections on Rabbits

2019 ◽  
Vol 43 (1) ◽  
pp. 34-42
Author(s):  
Afaf Abdulrahman Yousif
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Total Protein ◽  
Post Infection ◽  
Clinical Signs ◽  
Alanine Transaminase ◽  
Infected Group ◽  
Control Group ◽  
Biochemical Tests ◽  
Liver And Kidney

The study  aimed to show the effect of experimental infection with E.coli O157:H7 on some liver and kidney function in adult rabbits. Twenty five domestic rabbits (males and females), aged 6-8 weeks and weighing 1500-2000g, had a negative fecal bacteriological culture of E.coli O157:H7 were used. The rabbits were randomly divided into two groups: infected group (15 rabbits), each animal was drenched  orally with 1ml of Phosphate buffer saline containing (2×109CFU) of E.coli O157:H7, while the control group (10 rabbits) were drenched orally (1ml) of Phosphate buffer saline, the clinical signs were daily observed for 30 days, recording of body weight and blood samples were collected at (0, 3, 15 and 30) days for serum   to evaluate  biochemical tests including Alanine transaminase, Aspartate aminotransaminase and alkaline phosphatase, creatinine, urea  and total serum protein. The results showed different clinical signs after inoculation, including diarrhea, lethargy, weight loss, inability to eat or drink, urine was cloudy or milky, while control group remains in normal condition. The serum biochemical tests in infected group showed a significant increase of three enzymes at 15, and 30 days,   the highest values of Alanine transaminase; Aspartate aminotransaminase and alkaline phosphatase showed at 30 days post infection as compared with the control group. Serum creatinine and urea recorded a significant increase (P< 0.05) at 30 day post infection as compared with control group. However, total protein revealed a significant decrease in the infected group, and a significant decrease was noticed at 15 day post infection, while the control group showed an increase in total protein co-ordinated with increase of body weight and progressing age. This study concluded that the infection with E. coli O157:H7may cause changes in liver and kidney functions which were more sever at 15,30 days post infection.

scholarly journals DOSE-DIFFERENTIATED CORRELATION INTERACTIONS BETWEEN INDICES OF HEMOGLOBIN EXCHANGE IN TURKEYS UNDER THE INFLUENCE OF THE PROBIOTIC FORMULATION VETOM 1.2

2020 ◽  
pp. 82-91 ◽  
Author(s):  
M. S. Yakovleva ◽  
N. S. Yakovleva ◽  
N. A. Gotovchikov ◽  
S. N. Tishkov ◽  
L. P. Ermakova
Keyword(s):  
Serum Albumin ◽  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Bacillus Amyloliquefaciens ◽  
Total Bilirubin ◽  
Inverse Correlation ◽  
Direct Bilirubin ◽  
Subsequent Decrease ◽  
Probiotic Preparation

The effect of the probiotic preparation Vetom 1.2 on the basis of Bacillus subtilis VKPM B-10641, Bacillus amyloliquefaciens VKPM B-10642 and Bacillus amyloliquefaciens VKPM B-10643 in different doses on the correlation interactions between the parameters of turkey hemoglobin exchange, blood erythrocytes and blood red blood cells direct and total bilirubin, total protein and serum albumin was studies. Correlations were calculated using Spearman formula, the reliability of correlations was checked by Student. The formulation was used for turkeys once a day for 30 days in doses of 12.5; 25; fifty; 75 and 100 mg / kg body weight. It was found that Vetom 1.2 in minimal doses leads to an inverse correlation between iron and total bilirubin, direct bilirubin, total protein and albumin, an increase in the concentration of the formulation leads to the disappearance of this effect with a gradual subsequent decrease in this indicator. Vetom 1.2 in doses up to 50 mg / kg leads to an inverse correlation between the concentrations of hemoglobin and direct bilirubin, total protein and serum albumin, at a dose of 75 mg / kg there is a direct correlation between these indicators, and at a dose of 100 mg / kg returns a negative correlation. The probiotic preparation Vetom 1.2 leads to a direct dependence of the concentration of bilirubins (total and direct) in serum on the concentrations of hemoglobin and red blood cells when used in most doses, except 75 mg / kg, which reduces the correlation.

scholarly journals Comparative Evaluation of Infected and NoninfectedAmblyomma tristeTicks withRickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
F. A. Nieri-Bastos ◽  
M. P. J. Szabó ◽  
R. C. Pacheco ◽  
J. F. Soares ◽  
H. S. Soares ◽  
...  
Keyword(s):  
Infected Group ◽  
Control Group ◽  
Control Groups ◽  
Rickettsia Parkeri ◽  
Amblyomma Triste ◽  
Vertebrate Hosts ◽  
Domestic Rabbits ◽  
And Control ◽  
Term Maintenance

The distribution ofRickettsia parkeriin South America has been associated withAmblyomma tristeticks. The present study evaluated under laboratory conditions two colonies ofA. triste: one started from engorged females that were naturally infected byR. parkeri(designated as infected group); the other started from noninfected females (designated as control group). Both colonies were reared in parallel for five consecutive generations. Tick-naïve domestic rabbits were used for feeding of each tick stage and generation.R. parkeriwas preserved by transstadial maintenance and transovarial transmission inA. tristeticks for five consecutive generations, because all tested larvae, nymphs, and adults from the infected group were shown by PCR to contain rickettsial DNA. All rabbits infested by larvae, nymphs, and adults from the infected group seroconverted, indicating that these tick stages were all vector competent forR. parkeri. Expressive differences in mortality rates were observed between engorged nymphs from the infected and control groups, as indicated by 65.9% and 92.4% molting success, respectively. Our results indicate thatA. tristecan act as a natural reservoir forR. parkeri. However, due to deleterious effect caused byR. parkerion engorged nymphs, amplifier vertebrate hosts might be necessary for natural long-term maintenance ofR. parkeriinA. triste.

scholarly journals Peritoneal fluid changes in horses subjected to small colon distension

2011 ◽  
Vol 31 (5) ◽  
pp. 367-373 ◽  
Author(s):  
Rafael R Faleiros ◽  
Delphim G Macoris ◽  
Carlos Henrique C Saquetti ◽  
Andrea C Aita ◽  
Anderson Farias ◽  
...  
Keyword(s):  
Total Protein ◽  
Peritoneal Fluid ◽  
Clinical Signs ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Control Group ◽  
Colon Obstruction ◽  
Before And After ◽  
Colon Distension ◽  
Biochemical Analyses

Intestinal devitalization in cases of small colon obstruction may be difficult to detect based only in clinical signs. The purpose was to serially evaluate blood and peritoneal fluid of horses subjected to small colon distension. Seventeen adult horses were allotted in three groups. In the small colon-distended group (DG, n=7) a surgically-implanted latex balloon was inflated to promote intraluminal small colon distension. In the shamoperated group (SG, n=5), the balloon was implanted but not inflated, and no surgery was done in the control group (CG, n=5). Blood and peritoneal fluid were sampled before and after (6 samples with a 30-minute interval) intestinal obstruction for cytological and biochemical analyses. No significant changes in clinical signs occurred within groups or across time during the experimental period. There were no statistical differences among SG and SG groups in hematologic and blood chemistry variables. Although total protein concentration and lactate dehydrogenase (LDH) activity in peritoneal fluid remained most of the time within reference values during the experimental period in all groups, increases from baseline values were detected in SG and DG groups. Such increases occurred earlier, progressively and with greater magnitude in the DG when compared with the SG (P<0.05). Increases from baselines values were also observed in total nucleated cells and neutrophils counts in the DG (P<0.05). In conclusion, distension of the equine small colon induced progressive subtle increases in total protein and LDH concentrations in the peritoneal fluid during the first hours. Serial evaluation of these variables in peritoneal fluid may be useful for early detection of intestinal devitalization in clinical cases of equine small colon obstruction.

MONIZEN FORTE FOR LAMBS PARASIT-IC DISEASES

2020 ◽  
Vol 2 ◽  
pp. 84-87
Author(s):  
E. S. Engasheva ◽  
◽  
V. I. Kolesnikov ◽  
Keyword(s):  
Clinical Signs ◽  
Live Weight ◽  
Control Group ◽  
Not Given ◽  
The North ◽  
Condi Tions ◽  
High Infection ◽  
Mucous Discharge ◽  
And Control ◽  
Experimental Group

The Monizen Forte test for parasitic sheep diseases was tested under production condi-tions on 460 lambs of the North Caucasian breed, with a live weight of 25-30 kg, with high infection with nematodes, cestodes and larvae of the nasopharyngeal gadfly (Oestrus ovis), which were given orally in a mixture with half the normal rate of concentrated feed, the test drug in a dose of 1 ml per 20 kg of the animal weight. Experienced (20 ani-mals) and control (10 animals) groups of lambs with an average live weight of 28 kg were selected from these lambs according to the principle of analogues. To the lambs of the control group, the drug was not given. 10 days after administration of the drug by clini-cal and coprological studies, it was found that the lambs of the experimental group, the intensity of nematode invasion decreased and the drug’s intensity (IE) was 96.7%, and the extensivity (EE) was 95%. The lambs of the experimental group before the introduction of the drug were 40% invaded with moniesia and 50% dictiocauli, and after using Monizen Forte they were released - the ex-tensibility (EE) was 100%. Lambs of the control (untreated) group, the intensity and extent of infection with nematodes and ces-todes remained at the same level as at the beginning of the experiment. All 10 lambs (50%) of the experimental group, the clinical signs of estrosis (sneezing, snorting, serous-mucous discharge from the nose) disap- peared, and the lambs of the control group have these clinical signs persisted.

scholarly journals Oral Administration of Lactobacillus Strains Isolated from Breast Milk as an Alternative for the Treatment of Infectious Mastitis during Lactation

2008 ◽  
Vol 74 (15) ◽  
pp. 4650-4655 ◽  
Author(s):  
E. Jiménez ◽  
L. Fernández ◽  
A. Maldonado ◽  
R. Martín ◽  
M. Olivares ◽  
...  
Keyword(s):  
Breast Milk ◽  
Clinical Signs ◽  
Control Group ◽  
Control Groups ◽  
Lactobacillus Salivarius ◽  
Probiotic Group ◽  
Efficient Alternative ◽  
The Mean ◽  
And Control ◽  
Infectious Mastitis

ABSTRACT In this study, 20 women with staphylococcal mastitis were randomly divided in two groups. Those in the probiotic group daily ingested 10 log10 CFU of Lactobacillus salivarius CECT5713 and the same quantity of Lactobacillus gasseri CECT5714 for 4 weeks, while those in the control one only ingested the excipient. Both lactobacillus strains were originally isolated from breast milk. On day 0, the mean staphylococcal counts in the probiotic and control groups were similar (4.74 and 4.81 log10 CFU/ml, respectively), but lactobacilli could not be detected. On day 30, the mean staphylococcal count in the probiotic group (2.96 log10 CFU/ml) was lower than that of the control group (4.79 log10 CFU/ml). L. salivarius CECT5713 and L. gasseri CECT5714 were isolated from the milk samples of 6 of the 10 women of the probiotic group. At day 14, no clinical signs of mastitis were observed in the women assigned to the probiotic group, but mastitis persisted throughout the study period in the control group women. In conclusion, L. salivarius CECT5713 and L. gasseri CECT5714 appear to be an efficient alternative for the treatment of lactational infectious mastitis during lactation.

scholarly journals Plasma Calcium Levels in Pregnant Asian Women

1976 ◽  
Vol 13 (1-6) ◽  
pp. 339-344 ◽  
Author(s):  
Nina Polanska ◽  
R. A. Dale ◽  
M. R. Wills
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Dietary Intake ◽  
Total Protein ◽  
Plasma Calcium ◽  
Asian Women ◽  
Control Group ◽  
Calcium Phosphorus ◽  
And Control ◽  
Endogenous Synthesis

Plasma calcium, phosphorus, alkaline phosphatase, total protein, and albumin were measured during pregnancy in a group of Asian women living in the south of England and in a control group. The Asian women had slightly lower mean plasma calcium concentrations than the control group. Exposure of Asian women to sunshine appeared to be low, and it was inferred that they probably had little endogenous synthesis of cholecalciferol as a result. Both Asian and control subjects ingested similarly low amounts of vitamin D. The Asian women consumed greater amounts of phytate and vegetable fibres than the controls, and it is suggested that these substances may reduce absorption of insoluble lipids (including cholecalciferol) and calcium in a setting where the dietary intake and endogenous synthesis of vitamin D are already borderline for the subjects' requirements.

scholarly journals Pathogenicity and Effects on Lymphoid Organs of Recent Moroccan Very Virulent Infectious Bursal Disease Virus in Broilers and SPF Chickens

2021 ◽  
Author(s):  
Charifa DRISSI TOUZANI ◽  
Imane MAAROUFI ◽  
Siham FELLAHI ◽  
Ikhlass EL BERBRI ◽  
Fatima-zohra SIKHT ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Clinical Signs ◽  
Total Mortality ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Control Group ◽  
Bursal Disease ◽  
Specific Pathogen ◽  
And Control

Abstract The aim of the current study is to evaluate the pathogenicity of recent infectious bursal disease virus (IBDV) (1/chicken/Morocco/IB19/2017) genetically characterized as vvIBDV belonging to genogroup 3.Two chicken lines, broiler and specific-pathogen-free (SPF) chickens, were inoculated by occulonasal route with 0.2 ml of the 105EID50 /ml of viral solution of IB19 vvIBDV strain at 29 days of age. The experimental monitoring was carried out during 10 days post challenge (dpc). The clinical signs stared on day 2 pc with maximum severity observed between 3 and 6 dpc. The total mortality rate reached 10% in broilers (group G1) and 93% in SPF (G3). The macroscopic lesions in broilers G1 was a marked hypertrophy of the bursa of Fabricius (BF) with slight haemorrhage observed between 2 to 4 dpc, followed by very pronounced atrophy observed on the 5 dpc. The post-mortem examinations of dead SPF birds (G3) revealed on 3 dpc very haemorrhagic BF with black cherry appearance in 80 % of dead birds. The mean Bursa/Body Index (BBI) of challenged broilers (G1) showed a decrease of 46% on day 9 pc compared to broilers control group (G2) indicating bursal atrophy. The microscopic lesions found in the BF on 3 dpc consisted mainly of inflammation with severe lymphoid depletion of the follicles. The evaluation of recent vvIBDV outbreak is very important to understand its epidemiology and will contribute to the efficient prevention and control of IBD.

Sign in / Sign up

Export Citation Format

Share Document