scholarly journals Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

2022 ◽  
Vol 82 ◽  
Author(s):  
Z. B. Keskin ◽  
H. Kahraman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Secondary Metabolites ◽  
Bacillus Cereus ◽  
Soil Bacteria ◽  
First Time

Abstract The effects of Calcium (Ca+2) on virulence and some parameters should be analyzed in this study. Pseudomonas aeruginosa Gram (-) and Bacillus cereus Gram (+) were used. Both bacteria are soil bacteria. In this study; the effect of Ca+2 on protease, amylase, LasB elastolytic assay, H2O2, pyorubin and biofilm on metabolites of these bacteria were investigated during 24 hour time. In this study, the effect of Ca+2 on the production of some secondary metabolites on P. aeruginosa and B. cereus was investigated and presented for the first time by us.

scholarly journals Effect of Ca+2 on P. aeruginasa and B. cereus metabolites

2020 ◽  
Author(s):  
Hüseyin Kahraman ◽  
Zeliş Budak Keskin
Keyword(s):  
Secondary Metabolites ◽  
Soil Bacteria ◽  
First Time

Abstract Background: The effects of Ca+2 on virulence and some parameters were tried to be analyzed in this study. P. aeruginosa and B. cereus were used as Gram (-) bacteria as a control and Gram (+) bacteria as a control. Both bacteria are soil bacteria. Results: Amylase; P. aeruginosa is 102% increase in 0.1 M Ca+2 concentrations and B. cereus caused an increase in 123%. Biofilm P. aeruginosa; 200% increase in 0.1 M Ca+2 concentrations. B. cereus caused a 252% increase in the presence of 0.15 M Ca+2. Las B; in accordance with B. cereus, Las B activity decreased 1.7 times more than with P. aeruginosa. H2O2; P. aeruginosa increased by 60% resistance. B. cereus caused only a 3% increase. Protease in P. aeruginosa increase of 220%. B. cereus caused a 548% increase. Pyorubin, a pigment, caused an increase in the presence of Ca+2. However, these increases are not seen as significant increases.Conclusion: In this study; the effect of Ca+2 on protease, amylase, LasB elastolytic assay, H2O2, pyorubin and biofilm was investigated in a 24 hour time. In the study we have conducted here, the effect of Ca+2 meat on the production of some secondary metabolites on Gram (-) P. aeruginosa and Gram (+) B. cereus was investigated for the first time. As a result, although there are many different related studies, they are not comparative as in ours. Therefore, our study has progressed in a different direction and it is a first in its field.

scholarly journals Nanotoxicity of 2D Molybdenum Disulfide, MoS2, Nanosheets on Beneficial Soil Bacteria, Bacillus cereus and Pseudomonas aeruginosa

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1453
Author(s):  
Michael Bae ◽  
Jun Kyun Oh ◽  
Shuhao Liu ◽  
Nirup Nagabandi ◽  
Yagmur Yegin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Molybdenum Disulfide ◽  
Soil Bacteria ◽  
Soil Formation ◽  
Bacterial Survival ◽  
Mos2 Nanosheets ◽  
Log Reduction ◽  
Bulk Mos2 ◽  
2D Nanosheets

Concerns arising from accidental and occasional releases of novel industrial nanomaterials to the environment and waterbodies are rapidly increasing as the production and utilization levels of nanomaterials increase every day. In particular, two-dimensional nanosheets are one of the most significant emerging classes of nanomaterials used or considered for use in numerous applications and devices. This study deals with the interactions between 2D molybdenum disulfide (MoS2) nanosheets and beneficial soil bacteria. It was found that the log-reduction in the survival of Gram-positive Bacillus cereus was 2.8 (99.83%) and 4.9 (99.9988%) upon exposure to 16.0 mg/mL bulk MoS2 (macroscale) and 2D MoS2 nanosheets (nanoscale), respectively. For the case of Gram-negative Pseudomonas aeruginosa, the log-reduction values in bacterial survival were 1.9 (98.60%) and 5.4 (99.9996%) for the same concentration of bulk MoS2 and MoS2 nanosheets, respectively. Based on these findings, it is important to consider the potential toxicity of MoS2 nanosheets on beneficial soil bacteria responsible for nitrate reduction and nitrogen fixation, soil formation, decomposition of dead and decayed natural materials, and transformation of toxic compounds into nontoxic compounds to adequately assess the environmental impact of 2D nanosheets and nanomaterials.

The impact of traditional plants and their secondary metabolites in the discovery of COVID-19 treatment

2020 ◽  
Vol 26 ◽  
Author(s):  
Shabana Bibi ◽  
Ayesha Sarfraz ◽  
Ghazala Mustafa ◽  
Zeeshan Ahmed ◽  
Muhammad Aurang Zeb ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Test System ◽  
Chemical Information ◽  
System Type ◽  
The Family ◽  
Comprehensive Search ◽  
Coronavirus Infection ◽  
The Impact ◽  
First Time ◽  
Plant Sources

Background: Coronavirus Disease-2019 belongs to the family of viruses which cause a serious pneumonia along with fever, breathing issues and infection of lungs for the first time in China and later spread worldwide. Objective: Several studies and clinical trials have been conducted to identify potential drugs and vaccines for Coronavirus Disease-2019. The present study listed natural secondary metabolites identified from plant sources with antiviral properties and could be safer and tolerable treatment for Coronavirus Disease-2019. Methods: A comprehensive search on the reported studies was conducted using different search engine such as Google scholar, SciFinder, Sciencedirect, Medline PubMed, and Scopus for the collection of research articles based on plantderived secondary metabolites, herbal extracts, and traditional medicine for coronavirus infections. Results: Status of COVID-19 worldwide and information of important molecular targets involved in COVID-19 is described and through literature search, is highlighted that numerous plant species and their extracts possess antiviral properties and studied with respect to Coronavirus treatments. Chemical information, plant source, test system type with mechanism of action for each secondary metabolite is also mentioned in this review paper. Conclusion: The present review has listed plants that have presented antiviral potential in the previous coronavirus pandemics and their secondary metabolites which could be significant for the development of novel and a safer drug which could prevent and cure coronavirus infection worldwide.

scholarly journals Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 133 ◽  
Author(s):  
Annika Jagels ◽  
Viktoria Lindemann ◽  
Sebastian Ulrich ◽  
Christoph Gottschalk ◽  
Benedikt Cramer ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Future Research ◽  
Structural Modifications ◽  
Metabolite Profiles ◽  
The Individual ◽  
First Time ◽  
Analytical Standards ◽  
Respective Species

The genus Stachybotrys produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct Stachybotrys species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different Stachybotrys strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of Stachybotrys species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with Stachybotrys growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research.

The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria

2021 ◽  
pp. 105062
Author(s):  
Feli Feliatra ◽  
Ummi Mardhiah Batubara ◽  
Yuana Nurulita ◽  
Iesje Lukistyowati ◽  
Jarot Setiaji
Keyword(s):  
Secondary Metabolites ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria

scholarly journals Soil and Leaf Nutrients Drivers on the Chemical Composition of the Essential Oil of Siparuna muricata (Ruiz & Pav.) A. DC. from Ecuador

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2949
Author(s):  
Juan I. Burneo ◽  
Ángel Benítez ◽  
James Calva ◽  
Pablo Velastegui ◽  
Vladimir Morocho
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Secondary Metabolites ◽  
Essential Oil ◽  
Chemical Elements ◽  
Climatic Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Leaf Nutrients ◽  
First Time

Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.

PENGUJIAN KONSORSIUM BAKTERI ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT BERCAK DAUN CERCOSPORA DAN VIRUS KUNING PADA TANAMAN CABAI MERAH BESAR (Capsicum annum L.) DI KECAMATAN DAMPIT KABUPATEN MALANG

2021 ◽  
Vol 9 (3) ◽  
pp. 107-114
Author(s):  
Diyana Lestari ◽  
Luqman Qurata Aini
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Duncan Multiple Range Test ◽  
Capsicum Annum ◽  
Range Test

Penyakit virus kuning dan bercak daun Cercospora merupakan dua penyakit penting yang menjadi kendala dalam budidaya cabai merah besar. Penelitian ini bertujuan untuk menguji konsorsium bakteri antagonis Pseudomonas aeruginosa dan Bacillus cereus dalam mengendalikan penyakit virus kuning dan bercak daun Cercospora pada cabai merah besar serta pengaruhnya terhadap pertumbuhan tanaman cabai merah besar sendiri. Penelitian dilaksanakan pada bulan Oktober 2020 - Desember 2020 di lahan tumpangsari bawang merah dan cabai, Desa Ubalan, Kecamatan Dampit, Kabupaten Malang. Persiapan penelitian meliputi survei lokasi, persiapan alat dan bahan, pengacakan perlakuan, dan penentuan tanaman sampel. Pelaksanaan penelitian meliputi pengaplikasian larutan isolat bakteri antagonis di lapang, pengamatan mingguan, dan pengolahan data. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) dengan 9 perlakuan dan 3 ulangan. Data dianalisis menggunakan sidik ragam ANOVA dan uji lanjut DMRT (Duncan Multiple Range Test) pada taraf 5% menggunakan software DSAASTAT. Konsorsium P7 (Pseudomonas aeruginosa + Bacillus cereus 13) lebih efektif dalam menekan penyakit virus kuning, sedangkan perlakuan konsorsium P8 (Bacillus cereus 12 + Bacillus cereus 13) lebih efektif dalam menekan penyakit bercak daun Cercospora. Perlakuan konsorsium tidak memberikan pengaruh terhadap tinggi dan jumlah daun. Namun, konsorsium P7 mampu meningkatkan bobot tanaman cabai meskipun secara statistik tidak berbeda dengan perlakuan lainnya.

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Studies on the Chemical Diversities of Secondary Metabolites Produced by Neosartorya fischeri via the OSMAC Method

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2772 ◽  
Author(s):  
You-Min Ying ◽  
Lu Huang ◽  
Ting Tian ◽  
Cui-Yu Li ◽  
Shi-Lei Wang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cancer Cell Line ◽  
2D Nmr ◽  
Natural Occurrence ◽  
Fungal Metabolites ◽  
Isolation And Identification ◽  
Late Step ◽  
Neosartorya Fischeri ◽  
The One ◽  
First Time

The One Strain Many Compounds (OSMAC) method was applied to explore the chemical diversities of secondary metabolites produced by Neosartorya fischeri NRRL 181. Four pyripyropenes 1–4, eight steroids 5–11, and four prenylated indole alkaloids 12–15, were obtained from the fungus cultured in petri dishes containing potato dextrose agar (PDA). 1,7,11-trideacetylpyripyropene A (1) and 1,11-dideacetyl pyripyropene A (2) were obtained and spectroscopically characterized (1D, 2D NMR, and HR-ESI-MS) from a natural source for the first time. It offered a sustainable source of these two compounds, which were usually used as starting materials in preparing pyripyropene derivatives. In addition, as compared with all the other naturally occurring pyripyropenes, 1 and 2 possessed unique acetylation patterns that did not follow the established late-step biosynthetic rules of pyripyropenes. The natural occurrence of 1 and 2 in the fungus implied that the timing and order of hydroxylation and acetylation in the late-step biosynthetic pathway of pyripyropenes remained to be revealed. The isolation and identification of 1–15 indicated that the OSMAC method could remarkably alter the metabolic profile and enrich the chemical diversities of fungal metabolites. Compounds 1–4 exhibited no obvious cytotoxicity against the triple-negative breast cancer cell line MDA-MB-231 as compared with taxol.

Sign in / Sign up

Export Citation Format

Share Document