scholarly journals Evaluation of Enterococcus faecalis, Staphylococcus warneri and Staphylococcus aureus species in adults with generalized chronic periodontitis

2017 ◽  
Vol 65 (2) ◽  
pp. 121-127
Author(s):  
Aretuza FRITOLI ◽  
Eduardo LOBÃO ◽  
Geisla Soares ◽  
Belén RETAMAL-VALDES ◽  
Magda FERES
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Chronic Periodontitis ◽  
Bacterial Species ◽  
Hybridization Technique ◽  
Periodontal Pathogens ◽  
Periodontal Health ◽  
Staphylococcus Warneri ◽  
Percentage Points

ABSTRACT Objective: To identify and quantify the levels of three bacterial species that have recently been identified as potential “new” periodontal pathogens (Enterococcus faecalis, Staphylococcus aureus and Staphylococcus warneri) in subjects with periodontal health and generalized chronic periodontitis. Methods: Thirty adults with generalized chronic periodontitis and 10 periodontally healthy were included in this study. Nine subgingival biofilm samples were collected per subject and individually analyzed by checkerboard DNA-DNA hybridization technique. Results: The mean levels of E. faecalis and S. warneri were higher in chronic periodontitis than in periodontal health (p<0.05). Furthermore, a higher percentage of subjects with periodontitis were colonized by the three species evaluated in comparison with healthy subjects (p<0.05). This represented a difference of 40 percentage points between the two groups, for E. faecalis (present in 90% of individuals with periodontitis and 50% of the healthy individuals) and S. warneri (100% and 60%, respectively), and 26 percentage points for S. aureus (86% and 60%, respectively). Conclusion: E. faecalis and S. warneri have the potential to be periodontal pathogens. The role of S. aureus was less evident, since this species was more prevalent and at relatively higher levels in health than the other two species. These data might guide future studies on the role of these microorganisms in the etiology of periodontitis and help to establish more effective treatments for these infections.

New Bacterial Species Associated with Chronic Periodontitis

2003 ◽  
Vol 82 (5) ◽  
pp. 338-344 ◽  
Author(s):  
P.S. Kumar ◽  
A.L. Griffen ◽  
J.A. Barton ◽  
B.J. Paster ◽  
M.L. Moeschberger ◽  
...  
Keyword(s):  
New Species ◽  
Chronic Periodontitis ◽  
Bacterial Species ◽  
Pcr Amplification ◽  
Periodontal Pathogens ◽  
Cloning And Sequencing ◽  
Periodontal Health ◽  
Oral Flora ◽  
Bacteroidetes Phylum ◽  
Species Specific

Recent investigations of the human subgingival oral flora based on ribosomal 16S cloning and sequencing have shown many of the bacterial species present to be novel species or phylotypes. The purpose of the present investigation was to identify potential periodontal pathogens among these newly identified species and phylotypes. Species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species. Associations with chronic periodontitis were observed for several new species or phylotypes, including uncultivated clones D084 and BH017 from the Deferribacteres phylum, AU126 from the Bacteroidetes phylum, Megasphaera clone BB166, clone X112 from the OP11 phylum, and clone I025 from the TM7 phylum, and the named species Eubacterium saphenum, Porphyromonas endodontalis, Prevotella denticola, and Cryptobacterium curtum. Species or phylotypes more prevalent in periodontal health included two uncultivated phylotypes, clone W090 from the Deferribacteres phylum and clone BU063 from the Bacteroidetes, and named species Atopobium rimae and Atopobium parvulum.

scholarly journals Pharmacological Role of Anions (Sulphate, Nitrate, Oxalate and Acetate) on the Antibacterial Activity of Cobalt(II), Copper(II) and Nickel(II) Complexes With Nicotinoylhydrazine-Derived ONO, NNO and SNO Ligands

1996 ◽  
Vol 3 (5) ◽  
pp. 211-217 ◽  
Author(s):  
Zahid Hussain Chohan ◽  
Abdur Rauf
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Metal Ion ◽  
Bacterial Species ◽  
Biologically Active ◽  
Schiff Base Ligands ◽  
Mixed Ligands ◽  
And Staphylococcus Aureus

Mixed ligands biologically active complexes of cobalt(II), copper(II) and nickel(II) with nicotinoylhydrazine-derived ONO, NNO and SNO donor schiff-base ligands having the same metal ion but different anions such as sulphate, nitrate, oxalate and acetate have been synthesised and characterised on the basis of their physical, analytical and spectral data. In order to evaluate the role of anions on their bioability, these ligands and their synthesised metal complexes with various anions have been screened against bacterial species such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and the title studies have proved a definative role of anions in increasing the biological activity

scholarly journals Effect of vitamin C supplementation on gingival bleeding

2018 ◽  
Vol 30 (3) ◽  
pp. 178
Author(s):  
Indra Mustika Setia Pribadi ◽  
Nunung Rusminah ◽  
Yanti Rusyanti ◽  
Anne Agustina Suwargiani
Keyword(s):  
Vitamin C ◽  
Chronic Periodontitis ◽  
Host Defence ◽  
Periodontal Pathogens ◽  
Defence Mechanisms ◽  
Periodontal Health ◽  
High Category ◽  
Gingival Bleeding ◽  
Before And After ◽  
Vitamin C Supplementation

Introduction: Periodontitis is a multifactorial disease caused by a disproportion between environmental factors such as periodontal pathogens and the host defence. Genetic factors, hormones, and nutrition may influence host defence mechanisms. One of the nutrition needed for periodontal health is vitamin C. This study was aimed to evaluate the effect of vitamin C supplementation on gingival bleeding. Methods: As much as ninety-eight patients with chronic periodontitis consisted of 42 males and 56 females, aged 30 – 59-years-old participated in this study. Gingival bleeding was assessed before and after 3 months administered with vitamin C supplementation. Gingival bleeding criteria was based on Van der Velden with tooth selection based on Ramfjörd. Result: Gingival bleeding before the administration of vitamin C supplementation was found in the high category at 4 patients (4.1%), medium at 36 patients (36.7%), and low at 58 patients (59.2%).  After 3 months administration of vitamin C supplementation, high category gingival bleeding was found in 2 patients (2%), medium in 16 patients (16.3%), and low in 80 patients (81.6%). Conclusion: There was an effect of vitamin C supplementation on gingival bleeding.Keywords: Chronic periodontitis, vitamin C supplementation, gingival bleeding.

scholarly journals Susceptibility of Vascular Implants to Colonization in vitro by Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa

2017 ◽  
Vol 66 (1) ◽  
pp. 125-129
Author(s):  
Witold Woźniak ◽  
Aleksandra Kozińska ◽  
Piotr Ciostek ◽  
Izabela Sitkiewicz
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Bacterial Species ◽  
Silver Ions ◽  
Pericardial Patch ◽  
Associated Bacteria ◽  
Omniflow Ii

We compared association of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Enterococcus faecalis with nine vascular implants after co-culture. Vascular implants were composed of various materials such as warp knitted polyester (with or without gelatin and silver ions), expanded polytetrafluoroethylene and biological materials – surface treated porcine pericardial patch and Omniflow II. The lowest overall number of associated bacteria was detected for polytetrafluoroethylene implants and porcine pericardial patch. The highest overall number of associated bacteria was detected for Omniflow II implant. The major source of variation, i.e. primary factor influencing colonization, is the implant type (56.22%), bacterial species is responsible for only 1.81%, and interaction of those two factors – 13.09% of variation.

scholarly journals Subgingival Microbial Profile And Production Of Proinflammatory Cytokines In Chronic Periodontitis

Folia Medica ◽  
2014 ◽  
Vol 56 (3) ◽  
pp. 152-160 ◽  
Author(s):  
Velichka T. Dosseva-Panova ◽  
Christina L. Popova ◽  
Vladimir E. Panov
Keyword(s):  
Porphyromonas Gingivalis ◽  
Proinflammatory Cytokines ◽  
Chronic Periodontitis ◽  
Bacterial Species ◽  
Cytokine Expression ◽  
The Body ◽  
Gingival Tissue ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Necrosis Factor Alpha

ABSTRACT This review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. It has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. It is accepted that this social disease is multifactorial in etiology, but the evidence in the literature suggests that the levels of specifi c Gram-negative organisms in subgingival plaque biofilm play a major role in the initiation and progression of the disease. Of the many bacterial species inhabiting the periodontal environment, three types - Porphyromonas gingivalis (PG), Treponema denticola (TD), Tannerella forsythia (TF) - are strongly associated with the initiation and progression of periodontitis. Microbiological studies suggest that Porphyromonas gingivalis should be considered a major etiologic agent. Currently, Porphyromonas gingivalis is strongly associated with the pathogenesis of chronic periodontitis. On the other hand, the presence of Aggregatibacter actinomycetemcomitans in patients with chronic periodontitis may be related to the severity of the disease and thus modify the therapeutic plan. The increased amount of periodontal pathogens in the subgingival area can activate a cascade of defense mechanisms of the body associated with the production of factors causing infl ammation and destruction, which suggests a correlation between the bacterial findings and the body response implemented by enhancing the local cytokine expression. Studies in the literature show that the presence of certain micro-organisms in the periodontal environment is associated to increased levels of proinflammatory cytokines in the gingival fluid and gingival tissue. These levels have been associated with destructive tissues response. There is little evidence in the literature on the correlation of the levels of periodontal pathogens of sites with different pocket depth with periodontal disease activity defined by the degree of the proinflammatory cytokine expression such as tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6 ).

scholarly journals Porphyromonas gingivalis and its impact on periodontal health and systemic diseases. A concise review.

2019 ◽  
Vol 6 (1) ◽  
pp. 31-34
Author(s):  
Pablo Jasma ◽  
Patricio Sánchez
Keyword(s):  
Porphyromonas Gingivalis ◽  
Vascular Inflammation ◽  
Bacterial Species ◽  
Low Frequency ◽  
Chronic Inflammatory Disease ◽  
Periodontal Pathogens ◽  
Periodontal Health ◽  
Concise Review ◽  
Cardiovascular Cells ◽  
Systemic Health

Periodontitis is defined as a multifactorial chronic inflammatory disease, associated to a dysbiotic biofilm and characterized by the progressive destruction of the periodontal attachment. Clinical studies have revealed the presence of 10 to 15 bacterial species that are potential periodontal pathogens in adults. From these, the most cited are Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Tannerella forsythia. The aim of this article is to review P. gingivalis’ characteristics and impact on periodontal and systemic health. Different studies have reported a relation between the presence of P. gingivalis and periodontal disease. P. gingivalis was one of the most frequently detected species in aggressive and chronic periodontitis. This is due to its unique ability to avoid the host’s immune response and contribute to the development of the destructive process. P. gingivalis, although only present in low frequency, is pathogenic because of its ability to induce dysbiotic microbial communities. There is more evidence that P. gingivalis might invade cardiovascular cells and tissues causing inflammation. It has been suggested that NLRP3 inflammasome plays a key role in the development of vascular inflammation and atherosclerosis. The repeated exposure to P. gingivalis, produces neuroinflammation, neurodegeneration and formation of intra and extracellular amyloid plaques, which are pathognomonic signs of Alzheimer’s disease.

scholarly journals Association of haptoglobin and natural resistance-associated macrophage protein 1 alleles with heme-consuming periodontal pathogens in chronic periodontitis and peri-implantitis: A pilot study

2020 ◽  
Vol 12 (1) ◽  
pp. 39-44
Author(s):  
Mahdi Kadkhodazadeh ◽  
Ahmad Reza Ebadian ◽  
Zahra Alizadeh Tabari ◽  
Reza Amid ◽  
Anahita Moscowchi
Keyword(s):  
Chronic Periodontitis ◽  
Cross Sectional Study ◽  
Natural Resistance ◽  
Hybridization Technique ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Macrophage Protein ◽  
Iranian Patients

Background. This study aimed to assess the association of haptoglobin (HP) and natural resistance-associated macrophage protein 1 (NRAMP1) alleles with the presence of heme-consuming periodontal pathogens in a group of Iranian patients with chronic periodontitis and peri-implantitis. Methods. This cross-sectional study evaluated 69 eligible chronic periodontitis and peri-implantitis patients selected from Shahid Beheshti Dental School. The periodontally diseased individuals had at least three teeth with clinical attachment loss of ≥3 mm and a probing pocket depth (PPD) of ≥3 mm in at least two quadrants. Peri-implant PPD of at least one site was ≥5 mm with or without suppuration and bleeding on probing. A plaque index of >20% and a radiographic crestal bone loss was present in at least one site around the implant. The paper point method was used for sampling from the deepest periodontal/peri-implant pocket of each tooth or implant for the DNA checkerboard hybridization technique. Statistical analyses were performed with PASW Statistics 18.0. The variables were presented as absolute and relative frequencies (%). Results. An Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) score of 1–2 was 5.8 times more frequent in HP 2, rs1723540 G, and rs2276631 G alleles. A Porphyromonas gingivalis (P. gingivalis) score of 1–2 was 4.8 times more common in the subjects carrying HP 2, rs1723540 G, and rs2276631 G alleles compared with HP 1, rs1723540 A, and rs2276631 A alleles. Conclusion. Within the limitations of this study, it seems that there was a relationship between HP and NRAMP1 allele frequencies and the presence of heme-consuming periodontal pathogens in the Iranian patients with chronic periodontitis and peri-implantitis evaluated in the present study.

scholarly journals The Ps and Qs of alarmone synthesis inStaphylococcus aureus

2019 ◽  
Author(s):  
Ning Yang ◽  
Shujie Xie ◽  
Nga-Yeung Tang ◽  
Mei Y. Choi ◽  
Ying Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Bacterial Species ◽  
Stringent Response ◽  
Biochemical Properties ◽  
Dependent Manner ◽  
Regulatory Domain ◽  
Rna Molecules ◽  
Cellular Survival ◽  
Secondary Messengers

AbstractDuring the stringent response, bacteria synthesize guanosine-3’,5’-bis(diphosphate) (ppGpp) and guanosine-5’-triphosphate 3’-diphosphate (pppGpp), which act as secondary messengers to promote cellular survival and adaptation. (p)ppGpp ‘alarmones’ are synthesized and/or hydrolyzed by proteins belonging to the RelA/SpoT Homologue (RSH) family. Many bacteria also encode ‘small alarmone synthetase’ (SAS) proteins (e.g. RelP, RelQ) which may also be capable of synthesizing a third alarmone: guanosine-5’-phosphate 3’-diphosphate (pGpp). Here, we report the biochemical properties of the Rel (RSH), RelP and RelQ proteins fromStaphylococcus aureus(Sa-Rel, Sa-RelP, Sa-RelQ, respectively). Sa-Rel synthesized pppGpp more efficiently than ppGpp, but lacked the ability to produce pGpp. However, Sa-Rel efficiently hydrolyzed all three alarmones in a Mn(II) ion-dependent manner. The removal of the C-terminal regulatory domain of Sa-Rel increased its rate of (p)ppGpp synthesisca. 10-fold, but had negligible effects on its rate of (pp)pGpp hydrolysis. Sa-RelP and Sa-RelQ efficiently synthesized pGpp in addition to pppGpp and ppGpp. The alarmone-synthesizing abilities of Sa-RelQ, but not Sa-RelP, were allosterically-stimulated by the addition of pppGpp, ppGpp or pGpp. The respective (pp)pGpp-synthesizing activities of Sa-RelP/Sa-RelQ were compared and contrasted with SAS homologues fromEnterococcus faecalis(Ef-RelQ) andStreptococcus mutans(Sm-RelQ, Sm-RelP). Results indicated that EF-RelQ, Sm-RelQ and Sa-RelQ were functionally-equivalent; but exhibited considerable variations in their respective biochemical properties, and the degrees to which alarmones and single-stranded RNA molecules allosterically stimulated their respective alarmone-synthesizing activities. The respective (pp)pGpp-synthesizing capabilities of Sa-RelP and Sm-RelP proteins were inhibited by pGpp, ppGpp and pppGpp. Our results support the premise that RelP and RelQ proteins may synthesize pGpp in addition to (p)ppGpp withinS. aureusand other Gram-positive bacterial species.

Role of antimicrobial peptides system in inflammatory periodontal diseases non-specific oral cavity protection

2021 ◽  
Vol 66 (7) ◽  
pp. 422-427
Author(s):  
N. V. Davidovich ◽  
N. V. Solovieva ◽  
A. S. Galieva ◽  
S. Yu. Lepeshkin ◽  
E. N. Bashilova ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Antimicrobial Peptides ◽  
Chronic Periodontitis ◽  
Pathogenic Bacteria ◽  
Periodontal Diseases ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Local Immunity ◽  
Microbial Invasion

The system of antimicrobial peptides (AMP) is one of the most ancient mechanisms of the macroorganism resistance to infectious pathogens invasion. The aim of the study was to determine the role of the antimicrobial peptides system and periodontal pathogenic markers in the development and progression of inflammatory periodontal diseases. Gingival pocket washes (91 samples in total) for the research were received from patients with inflammatory periodontal diseases (chronic periodontitis and gingivitis) and intact periodontium. Using ELISA, the content of antimicrobial peptides was determined: human alpha-defensin (HNP 1-3), beta-defensin (HBD 1-3) and cathelicidin (LL-37). Periodontal pathogenic markers were isolated during RT-PCR. The study revealed differences in AMP concentrations by groups: level of HBD 2 in patients with chronic periodontitis was 1,36 times higher than those in the group of patients with chronic gingivitis (p=0,023) and 2,39 times higher than those in the control group (p<0,001), the content of HNP 1-3 in the group of patients with chronic periodontitis was reduced by 1,23 times compared with the indicators of the group of patients with gingivitis (p=0,045) and by 1,97 times compared with the indicators of the control group (p<0,001). The frequency of detection of periodontal pathogenic bacteria genes was 88,0% in patients with periodontitis, 76,92% in patients with gingivitis and 33,3% in the group with intact periodontium. HBD 2 content moderately correlated with the definition of P. gingivalis (r=0,612; p=0,022), T. forsythensis (r= 0,434; p=0,015), A. actinomycetemcomitans (r=0,483; p=0,006), a moderate negative correlation was detected between the content of HNP 1-3 and the release of periodontal pathogens in associations (P. gingivalis with T. forsythensis and T. denticola) (r=-0,388; p=0,031) in the group of patients with chronic periodontitis. Thus, the revealed relationships and correlations indicate shifts in the processes of reparative regeneration of the oral cavity and the regulation of local immunity in response to microbial invasion.

scholarly journals Role of MurT C-Terminal Domain in the Amidation of Staphylococcus aureus Peptidoglycan

2019 ◽  
Vol 63 (10) ◽  
Author(s):  
Bárbara V. Gonçalves ◽  
Raquel Portela ◽  
Ricardo Lobo ◽  
Teresa A. Figueiredo ◽  
Inês R. Grilo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Multiple Roles ◽  
Great Effort ◽  
Content Type ◽  
Terminal Domain ◽  
Oxacillin Resistance ◽  
Amidation Reaction

ABSTRACT Glutamate amidation, a secondary modification of the peptidoglycan, was first identified in Staphylococcus aureus. It is catalyzed by the protein products of the murT and gatD genes, which are conserved and colocalized in the genomes of most sequenced Gram-positive bacterial species. The MurT-GatD complex is required for cell viability, full resistance to β-lactam antibiotics, and resistance to human lysozyme and is recognized as an attractive target for new antimicrobials. Great effort has been invested in the study of this step, culminating recently in three independent reports addressing the structural elucidation of the MurT-GatD complex. In this work, we demonstrate through the use of nonstructural approaches the critical and multiple roles of the C-terminal domain of MurT, annotated as DUF1727, in the MurT-GatD enzymatic complex. This domain provides the physical link between the two enzymatic activities and is essential for the amidation reaction. Copurification of recombinant MurT and GatD proteins and bacterial two-hybrid assays support the observation that the MurT-GatD interaction occurs through this domain. Most importantly, we provide in vivo evidence of the effect of substitutions at specific residues in DUF1727 on cell wall peptidoglycan amidation and on the phenotypes of oxacillin resistance and bacterial growth.

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