scholarly journals Conditioning and coating of Urochloa brizantha seeds associated with inoculation of Bacillus subtilis1

2019 ◽  
Vol 49 ◽  
Author(s):  
Antônio Emilio Rodrigues Manrique ◽  
Rita de Cássia Lima Mazzuchelli ◽  
Ademir Sergio Ferreira Araujo ◽  
Fabio Fernando de Araujo
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Seed Storage ◽  
Forage Plants ◽  
Technological Advances ◽  
Randomized Design ◽  
Urochloa Brizantha ◽  
Shoot Mass ◽  
And Control ◽  
And Storage

ABSTRACT Technological advances for improving the sowing stage of forage plants are important because, besides increasing the operation efficiency, they may also positively affect the plant growth. This study aimed to evaluate the inoculation of Bacillus subtilis in Urochloa brizantha seeds, using coating, physiological conditioning and storage procedures, as well as to observe its effects on seed storage and plant growth. A completely randomized design, in a 2 × 3 × 5 + 1 factorial scheme [two inoculation procedures (coating and conditioning), three inoculants (B. subtilis AP-3, B. subtilis PRBS-1 and without bacteria), five storage periods (0, 30, 60, 90 and 120 days) and control (bare seeds)], with five replicates, was used. Two lots of U. brizantha seeds were used, characterized as being of higher and lower viability. After the treatments, part of the seeds was employed to evaluate the plant growth promotion in a greenhouse, during 180 days, while the remaining portion was stored at a temperature of 25 ºC, with the seed germination being evaluated every 30 days, for 120 days. The physiological conditioning showed the best performance in the evaluations carried out for U. brizantha seeds with B. subtilis. The coating and conditioning of the U. brizantha seeds with lower viability, both with and without the inoculation of bacteria, increases the shoot mass of plants after 180 days of growth.

scholarly journals Control of Seed-borne Disease (Octomeles Sumatrana) during Storage

2020 ◽  
Vol 8 (2) ◽  
pp. 133-144
Author(s):  
NFN Nurhasybi ◽  
◽  
Tati Suharti ◽  
Keyword(s):  
Raw Materials ◽  
Seed Viability ◽  
Seed Storage ◽  
Forest Tree ◽  
Storage Room ◽  
Natural Fungicide ◽  
Alternative Species ◽  
Seed Handling ◽  
And Control ◽  
And Storage

The forest tree species that are widely grown to produce pulp and paper raw materials are Acacia spp. and Eucalyptus spp. One alternative species is binuang bini (Octomeles sumatrana) which can be developed for plantations. To maintain high seed viability, it is necessary to know the handling of seeds and control of seed-borne diseases during storage. The purpose of this study was to determine the method of the effective technique to control the seed-borne diseases of binuang bini during seed storage. The techniques for Seed disease kontrol methods in seed storage are carried out using chemical and natural fungicide. The results showed that the pure live seed was influenced by a single factor of fungicide and storage room, interactions between fungicides and storage room, interactions between fungicides and storage periods as well as interactions between fungicides and storage space and storage periods. Seed handling of binuang bini can be conducted effectively and efficiently by storing the seed in refrigerator for 3 months without using fungicides, but it will be better if the seed is given benomil fungicide and stored in airconditioned room (temperature of 18℃-20℃ and relative humidity of 50%-60 %).

scholarly journals Seed treatment using rhizobacteria as plant growth promotion of two chili variety (Capsicum annuum L.)

2022 ◽  
Vol 951 (1) ◽  
pp. 012060
Author(s):  
Syamsuddin ◽  
Halimursyadah ◽  
Samingan ◽  
V Maulidia
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Plant Growth Promotion ◽  
Vegetative Growth ◽  
Seed Treatment ◽  
Growth Promotion ◽  
Growth And Yield ◽  
Capsicum Annuum L ◽  
Randomized Design ◽  
Chili Peppers

Abstract The research aims to find out the effect of pre-germination treatment seeds using rhizobacteria as plant growth promotion of two varieties of red chili peppers in the field. The experiment used a randomized design of factorial groups. Factors studied were varieties (V) and rhizobacteria types (R). The variety factor consists of 2 varieties namely PM999 (V1) and Kiyo F1 (V2). While the type of rhizobacteria factor tried consists of 8 treatment, namely, control (R0), Azotobacter sp. (R1), B. megaterium (R2), P. atmuta (R3), B. alvei (R4), Flavobacterium sp. (R5), B. coagulans (R6), B. firmus (R7) and B. pilymixa (Rs). Each treatment was repeated 3 times, so there were 48 experimental units. Each unit of experiment is represented by 5 sample plants. The data was analyzed using ANOVA and continued with DMRT test at real level α = 0.05. The results showed that vegetative growth and production of chili plants until the age of 45 days after planting in each variety is not dependent on the pre-germination treatment of seeds with rhizobacteria. But the varieties of chili plants used affect vegetative growth and production. PM999 varieties are superior to the Kiyo F1 variety. Pre-germination treatment of seeds using rhizobacteria is relatively effective in improving vegetative growth and yield of chili plants. Among the 8 isolates rizobacteria isolate Azotobacter sp., B. megaterium, B. coagulants, Flavobacterium sp., and P. atmuta relatively effective to provide an increased effect on the growth and production of chili plants.

scholarly journals Influence of Bacillus Spp. Based Bioproducts on Potato Plant Growth and Control of Rhizoctonia Solani

2019 ◽  
Vol 76 (1) ◽  
pp. 29
Author(s):  
Sorina DINU ◽  
Oana Alina BOIU-SICUIA ◽  
Florica CONSTANTINESCU
Keyword(s):  
Plant Growth ◽  
Rhizoctonia Solani ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Aqueous Suspension ◽  
Potato Plants ◽  
Bacillus Spp ◽  
Seeding Material ◽  
Biocontrol Potential ◽  
And Control

Some Bacillus based bioproducts were analyzed for their plant growth promotion and Rhizoctonia solani biocontrol potential in potato plants. The bioproducts were formulated as concentrated aqueous suspension, each containing one of the following plant beneficial bacteria: Bacillus safensis Rd.b2, Bacillus spp. 75.1s and Cp.b4 strains. These were applied on potato seeding material in order to evaluate plant growth promotion effects. The biocontrol efficacy was also evaluated, using Rhizoctonia solani DSM 63002 as plant pathogen, and Prestige 290FS as reference chemical treatment.In the plant growth-promotion experiments, several biologic parameters were biometrically evaluated. Best results regarding plant growth and vigor were obtained using CropMax, a commercial phytostimulatory product. However, the bacterial treatment with Bacillus spp. Cp.b4 and 75.1s showed an improved plant growth compared to the untreated control. An efficacy of 93.75% against Rhizoctonia dumping-off was registered when using the Prestige 290FS chemical control. Mix treatments based on this pesticide, in low dose, combined with Cp.b4 or 75.1s biocontrol strains significantly reduced the pathogenic attack, showing 85 to 87.5% efficacy.The present research demonstrated that the bacterial bioproducts based on Bacillus spp. 75.1s and Cp.b4 strains increase plant growth and are highly effective in controlling Rhizoctonia attack in potato plants.

scholarly journals Seed quality response of Urochloa humidicola cv. Llanero to drying surfaces and environments1

2018 ◽  
Vol 48 (3) ◽  
pp. 306-315
Author(s):  
Alan Mario Zuffo ◽  
Joacir Mario Zuffo Júnior ◽  
Everton Vinicius Zambiazzi ◽  
Fábio Steiner
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Seed Storage ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Quality Response ◽  
And Storage

ABSTRACT The drying process and storage may interfere in the quality of creeping signalgrass seeds [Urochloa humidicola (Rendle.) Morrone & Zuloago, syn. Brachiaria humidicola (Rendle.) Schweick.]. This study aimed to evaluate the physical, physiological and sanitary quality of creeping signalgrass cv. Llanero seeds submitted to drying surfaces and environments and stored for two periods (1 and 8 months). The experiment was arranged in a completely randomized design, in a 4 × 2 × 2 + 2 factorial arrangement, with four drying surfaces (soil, asphalt, concrete and ceramic floor), two drying environments (seeds exposed to the sun or shade), two seed storage periods (1 and 8 months) and two additional treatments with drying in paper bags under laboratory conditions (control) and stored for 1 or 8 months, with four replicates. The water content, viability by the tetrazolium test, electrical conductivity, germination rate, germination rate index and seed sanitary quality were measured. Drying at full sun impaired the physiological quality of the seeds due to the high temperatures (46.8-51.0 ºC) of the drying surface; however, reduced the incidence of pathogens. The drying of seeds in paper bags under shade results in a higher physiological quality, but the sanitary quality is compromised. However, the pathogens present in the seeds did not inhibit their viability and vigor. The storage for eight months reduces the seed quality.

scholarly journals Rhizospheric Fungi to Plant Growth Promotion: A Review

2021 ◽  
Vol 9 (1) ◽  
pp. 411
Author(s):  
João Manoel da Silva ◽  
Yamina Coentro Montaldo ◽  
Arthur Costa Pereira Santiago de Almeida ◽  
Viviane Araújo Dalbon ◽  
Juan Pablo Molina Acevedo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Growth Regulators ◽  
Indole Acetic Acid ◽  
Growth Promotion ◽  
Direct Influence ◽  
Biological Interactions ◽  
Ecological Role ◽  
And Control ◽  
Stimulation Of

The rhizosphere is the region that has direct influence from the roots. This is the place where most of the interactions between microorganisms and plants occur. Studies involving the ecology of microbial communities from the rhizosphere became more frequent after the first reports of biological interactions of microorganisms with plants that influence physically and chemically modify the soil surrounding. According to these hypotheses, the rhizosphere mycobiota provides the development of plants through various mechanisms, direct and indirect. Thus, the objective of this review was to explain the aspects that provide characterizing these microorganisms as beneficial to plants in view of their applicability to agro-ecosystems. Therefore, it is stated that rhizospheric fungi have the solubilization of phosphorus (P), assimilating this nutrient for plants, promoting growth through the production or stimulation of the production of growth regulators such as 3-indole acetic acid, and control of phytopathogenic agents such as other filamentous fungi, and also phytonemamatodes. Therefore, it is possible to observe the importance of the constant observance of the action of these microorganisms in terms of their ecological role due to the agro-ecosystem.

scholarly journals Growth promotion and productivity of lettuce using Trichoderma spp. commercial strains

2019 ◽  
Vol 37 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Fabíola T Pereira ◽  
Jessica B de Oliveira ◽  
Paulo Henrique PC Muniz ◽  
Gustavo Henrique S Peixoto ◽  
Roberli R Guimarães ◽  
...  
Keyword(s):  
Field Experiment ◽  
Growth Promotion ◽  
Dry Mass ◽  
Mass Ratio ◽  
Trichoderma Spp ◽  
Randomized Design ◽  
Growth Assay ◽  
Lettuce Growth ◽  
Lettuce Yield ◽  
Shoot Mass

ABSTRACT The aim of this study was to evaluate four strains of Trichoderma spp. (T. harzianum IBLF 006 WP, T. harzianum IBLF 006 SC, T. harzianum ESALQ 1306 and T. asperellum URM 5911) for seedling growth promotion in laboratory and head lettuce yield in field conditions. The experiment was carried out in a completely randomized design with four treatments (strains): IBLF 006 WP, IBLF 006 SC, ESALQ 1306 and URM 5911 and a non-inoculated (without Trichoderma) control. Each treatment consisted of 200 seeds, arranged in four replicates. Lettuce seeds cv. Astra were treated with 2 mL Trichoderma suspension (2.5 x 108 conidia mL-1 per each 100 g seeds) and submitted to growth assay in laboratory up to 7 days after sowing. For field experiment, we opened furrows, which were manually sprayed with 5 x 107 conidia mL-1. Afterwards, seedlings were transplanted (4 to 6 leaves of head lettuce cv. Mauren) and harvested 40 days later. Each treatment consisted of four replicates (1.2 x 1.2 m, 16 plants per plot) arranged in randomized blocks. In both experiments, a control without Trichoderma application was included, and we evaluated shoot length, root and total length, shoot, root and total fresh mass and shoot, root and total dry mass, shoot mass ratio, root mass ratio and shoot/root ratio. The germination (%) was evaluated by laboratory tests, whereas in field experiment, height, stem diameter, head diameter, number of leaves and yield were evaluated. The T. harzianum strain ESALQ 1306 provided the best head lettuce growth rate in laboratory test, which was confirmed in field experiment, in which the productivity (50.2 t ha-1) was superior when compared to the other strains (41.38 to 44.23 t ha-1) and the control (30.18 t ha-1).

Opposing effects of bacterial endophytes on biomass allocation of a wild donor and agricultural recipient

2020 ◽  
Vol 96 (3) ◽  
Author(s):  
Rachel L Rubin ◽  
Ashley N Jones ◽  
Michaela Hayer ◽  
Molly E Shuman-Goodier ◽  
Lela V Andrews ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Elevation Gradient ◽  
Bouteloua Gracilis ◽  
Blue Grama ◽  
Root Endophytes ◽  
Ecological Features ◽  
Shoot Mass ◽  
Opposing Effects ◽  
Root Microbiome

ABSTRACT Root endophytes are a promising tool for increasing plant growth, but it is unclear whether they perform consistently across plant hosts. We characterized the blue grama (Bouteloua gracilis) root microbiome using two sequencing methods, quantified the effects of root endophytes in the original host (blue grama) and an agricultural recipient, corn (Zea mays), under drought and well-watered conditions and examined in vitro mechanisms for plant growth promotion. 16S rRNA amplicon sequencing revealed that the blue grama root microbiome was similar across an elevation gradient, with the exception of four genera. Culturing and Sanger sequencing revealed eight unique endophytes belonging to the genera Bacillus, Lysinibacillus and Pseudomonas. All eight endophytes colonized corn roots, but had opposing effects on aboveground and belowground biomass in each plant species: they increased blue grama shoot mass by 45% (19) (mean +/− SE) while decreasing corn shoot mass by 10% (19), and increased corn root:shoot by 44% (7), while decreasing blue grama root:shoot by 17% (7). Furthermore, contrary to our expectations, endophytes had stronger effects on plant growth under well-watered conditions rather than drought conditions. Collectively, these results suggest that ecological features, including host identity, bacterial traits, climate conditions and morphological outcomes, should be carefully considered in the design and implementation of agricultural inocula.

scholarly journals Effect of polyethylene glycol 6000 and storage period on seed quality of cocoa (Theobroma cacao L.)

2014 ◽  
Vol 30 (1) ◽  
Author(s):  
Astiti Rahayu ◽  
Triani Hardiyati ◽  
Ponendi Hidayat
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Storage Period ◽  
Seed Storage ◽  
Peg 6000 ◽  
Randomized Design ◽  
Long Time ◽  
Physiological Quality ◽  
And Storage

Increased productivity of cocoa needs high quality of cocoa seeds which are generally provided by certified seed gardens located far from smallholders farm, where seed delivery takes long time and may reduce physiological quality of seeds. One effort to maintain the seed quality is by treatment the seeds with polyethylene glycol6000 (PEG 6000). This study was aimed to study the interaction of PEG 6000 concentration and storage period on cocoa seed quality, and to determine the best concentration of PEG 6000 and storage period to maintain cocoa seed quality. The research was conducted in seed storage room, seed laboratory and green houseof PPPPTK, Cianjur. The experimental design used was a factorial completely randomized design and each combination treatment repeated three times. The first factor was concentration of PEG 6000 of 0%, 20%, 40%, and 60%, and the second factor was the storage period of 3 weeks, 6 weeks, 9 weeks, and 12 weeks. The results showed that concentration of PEG 60% with three weeks storage period was able to minimize the number of moldy seeds. The concentration of PEG 20%, 40%, and 60% were able to prevent seed germination in storage until six weeks. Concentration of PEG 6000 20% was able to maintain moisture content, seed germination, and germination rate.Key words:cocoa seed, storage, recalcitrant, PEG

scholarly journals Complete Genome Sequences of Endophytic Bacilli Isolated from Grapevine Plants

2019 ◽  
Vol 8 (48) ◽  
Author(s):  
Andrey V. Mardanov ◽  
Elena P. Chizhevskaya ◽  
Alexander M. Lazarev ◽  
Andrey L. Rakitin ◽  
Alexey V. Beletsky ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Bacillus Amyloliquefaciens ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Antimicrobial Compounds ◽  
Genome Sequences ◽  
Content Type ◽  
Complete Genomes ◽  
And Control

The endophytic strains Bacillus amyloliquefaciens V417 and V167 were isolated from cultured grape plants. We sequenced the complete genomes of these strains to reveal their potential beneficial properties for plant growth promotion and control of fungal pathogens. Genes responsible for the synthesis of antimicrobial compounds and siderophores were identified.

Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria

2007 ◽  
Vol 53 (2) ◽  
pp. 159-167 ◽  
Author(s):  
J.W. Kloepper ◽  
A. Gutiérrez-Estrada ◽  
J.A. McInroy
Keyword(s):  
Plant Growth ◽  
Induced Resistance ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Leachate Quality ◽  
Shoot Mass

For several years, we have noticed that plant growth-promoting rhizobacteria (PGPR), which consistently promote plant growth in greenhouse tests during spring, summer, and fall, fail to elicit plant growth promotion during the midwinter under ambient light conditions. This report tests the hypothesis that photoperiod regulates elicitation of growth promotion and induced systemic resistance (ISR) by PGPR. A commercially available formulation of PGPR strains Bacillus subtilis GB03 and Bacillus amyloliquefaciens IN937a (BioYield®) was used to grow tomato and pepper transplants under short-day (8 h of light) (SD) and long-day (12 h of light) (LD) conditions. Results of many experiments indicated that under LD conditions, BioYield consistently elicited significant increases in root and shoot mass as well as in several parameters of root architecture. However, under SD conditions, such increases were not elicited. Differential root colonization of plants grown under LD and SD conditions and changes in leachate quality partially account for these results. BioYield elicited ISR in tomato and pepper under both LD and SD conditions, indicating that although growth promotion was not elicited under SD conditions, induced resistance was. Overall, the results indicate that PGPR-mediated growth promotion is regulated by photoperiod, while ISR is not.

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