scholarly journals Optimization and sensitivity analysis of fast ethanol assay in maize seeds

2019 ◽  
Vol 41 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Damrongvudhi Onwimol ◽  
Thunyapuk Rongsangchaicharean ◽  
Pitipong Thobunluepop ◽  
Tanapon Chaisan ◽  
Wanchai Chanprasert
Keyword(s):  
Quality Assessment ◽  
Ethanol Production ◽  
Seed Quality ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Maize Seed ◽  
Seed Deterioration ◽  
Maize Seeds ◽  
Hermetic Seal

Abstract: The evaluation of seed deterioration is very important to control the quality of the seeds stored. This study aimed to investigate the potential of fast ethanol assay for seed quality assessment of maize stored under different conditions. The first experiment was to determine the incubating temperature, incubating time, and amount of seed used in the assay. The results showed that the best protocol for the detection of headspace ethanol was incubation of 3 g of maize seed with 20% moisture content (wet basis) in a 20 mL gas chromatography vial at 70 °C for 1.5 h. The assay induced approximately 200-700 µg.L-1 of headspace ethanol, which was sufficient to identify seeds with different vigour levels. In the second experiment, the optimal conditions were used for quality assessment in aged maize seed stored for 12 months under different storage conditions. The increase in the ethanol production of stored maize seed under the controlled conditions (15 °C and 20% RH in the hermetic seal) was lower than under ambient conditions. The ethanol production levels of maize seed samples at the start of storage was significantly lesser than at six months storage (p < 0.05). The test limitations in deteriorated seed with different cultivars and ages will be discussed.

A fast ethanol assay to detect seed deterioration

2011 ◽  
Vol 22 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Jan Kodde ◽  
Wayne T. Buckley ◽  
Corine C. de Groot ◽  
Marie Retiere ◽  
Ana Marcela Viquez Zamora ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Elevated Temperatures ◽  
Seed Quality ◽  
Inverse Correlation ◽  
Hot Water ◽  
Ambient Conditions ◽  
Simple Method ◽  
Seed Deterioration ◽  
Modified Method ◽  
Water Treatments

AbstractThe most common way to test seed quality is to use a simple and reliable but time- and space-consuming germination test. In this paper we present a fast and simple method to analyse cabbage seed deterioration by measuring ethanol production from partially imbibed seeds. The method uses a modified breath analyser and is simple compared to gas chromatographic or enzymatic procedures. A modified method using elevated temperatures (40°C instead of 20°C) shortened the assay time and improved its sensitivity. The analysis showed an inverse correlation between ethanol production and seed quality (e.g. the final percentages or speed of germination and the number of normal seedlings). The increase in ethanol production was observed when cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings. Premature seeds produced more ethanol upon imbibition than mature seeds. Ethanol production occurred simultaneously with oxygen consumption, indicating that lack of oxygen is not the major trigger for ethanol production.

scholarly journals Effect of production environments on storage and physiological quality of maize seed

2021 ◽  
Vol 8 ◽  
pp. 1-15
Author(s):  
Itala Thaísa Padilha Dubal ◽  
Ivan Ricardo Carvalho ◽  
João Roberto Pimentel ◽  
Cristian Troyjack ◽  
Vinícius Jardel Szareski ◽  
...  
Keyword(s):  
Dry Matter ◽  
Seed Quality ◽  
Maize Seed ◽  
Large Format ◽  
Maize Seeds ◽  
Production Environments ◽  
Physiological Quality ◽  
Large Round ◽  
Physiological Characters

Storage is a factor that can affect seed quality as a function of latent damages. The losses that most contribute to the reduction in germination and vigor during storage are those caused by humidity, mechanical and temperature-related, that may interfere with seed quality throughout storage. The aim of this study was evaluating the physiological quality of maize seeds produced in two environments and classified in different formats compared to storage periods. The experiment was carried out in the 2016 harvest in two cultivation environments: Entre-Ijuís, RS and Pelotas, RS. Seeds were stratified by thickness through sieves with oblong holes and stratified into small round, large round, small flat and large flat. Growing environments influence the physiological characteristics of maize seeds. The flat and large format seeds have superior physiological quality for the measured physiological characters. The small and round seeds present lower values for germination, first germination count, emergence speed index, shoot dry matter of emergence seedlings and shoot dry matter of germination seedlings. Corn seeds when stored under controlled conditions remain for long periods without causing decreases in physiological quality.

scholarly journals PHYSIOLOGICAL AND SANITARY QUALITIES OF MAIZE LANDRACE SEEDS STORED UNDER TWO CONDITIONS

2015 ◽  
Vol 39 (4) ◽  
pp. 339-347 ◽  
Author(s):  
Raquel Stefanello ◽  
Marlove Fátima Brião Muniz ◽  
Ubirajara Russi Nunes ◽  
Cláudia Braga Dutra ◽  
Iana Somavilla
Keyword(s):  
Seed Quality ◽  
Accelerated Aging ◽  
Storage Period ◽  
Cold Test ◽  
Maize Seed ◽  
Maize Landrace ◽  
Maize Seeds ◽  
Plastic Bag ◽  
Physiological Quality

The preservation of seed quality during the storage period depends not only on the conditions during production and harvesting but also on the storage and maintenance of appropriate storage product conditions. Thus, the aim of this study was to evaluate the physiological and sanitary qualities of maize landrace seeds stored under two conditions. The maize seed batch varieties Oito carreiras, Cabo roxo and Lombo baio were used. Tests included germination, first count, cold test, accelerated aging and sanity. Based on the results it was concluded that the physiological quality of these seed varieties decreased with the storage period. The major fungi identified in the maize seeds during storage were from the genera Aspergillus, Fusarium and Penicillium, which caused deterioration and reduction of the physiological quality. Storage using a paper bag at a temperature of 10 °C did not prevent the deterioration of maize seeds but was more effective at preserving the quality of the seed compared with a plastic bag at room temperature.

scholarly journals CNSL Concentration and Natural Dyes Effects in Formulation of Botanical Pesticide on Sitophilus zeamais and Maize Seed Quality

2020 ◽  
Vol 4 (1) ◽  
pp. 1-9
Author(s):  
Dian Astriani ◽  
Wafit Dinarto ◽  
Aji Jatmiko
Keyword(s):  
Seed Quality ◽  
Sitophilus Zeamais ◽  
Natural Dyes ◽  
Maize Weevil ◽  
Maize Seeds ◽  
Botanical Pesticide ◽  
Randomized Design ◽  
Efficiency And Effectiveness ◽  
Push Down

Maize is an important commodity in Indonesia and is vulnerable to maize weevil (Sitophilus zeamais) during storage which can cause a 100% yield loss. Chasew Nut Shell Liquid (CNSL) is a botanical pesticide able to control S. zeamais. Increasing CNSL efficiency and effectiveness requires additional adjuvants, such as solvents and dyes. Natural dyes addition is intended to provide seed markers. This study aimed to determine the best CNSL and natural dye formulation to control maize weevil and maintain maize seeds quality during storage. The research was conducted from August to October 2018 at the Agronomy Laboratory, Faculty of Agroindustry, Yogyakarta Mercu Buana University. The research was a single factor consisted of seven treatments with four replications arranged in a completely randomized design (CRD). Treatments were combinations of CNSL concentrations with the addition of natural dyes from suji leaf and teak leaves with one untreated control. The results of the research showed that CNSL as a seed treatment botanical pesticide could cause mortality and suppress S. zeamais populations and maintain the quality of maize seeds during storage for 12 weeks. Treatments with 20% CNSL formulation concentration and the addition of natural dyes both leaf pleomele or teak leaves could cause higher mortality and push down more populations and also could better maintain seed quality than 10 and 0% CNSL formulation concentrations.

Physiology of Oil Seeds. X. Seed Quality of Peanut Genotypes as Affected by Ambient Storage Temperature1

1992 ◽  
Vol 19 (2) ◽  
pp. 72-77 ◽  
Author(s):  
Darold L. Ketring
Keyword(s):  
Ambient Temperature ◽  
Seedling Growth ◽  
Seed Quality ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Ambient Storage ◽  
Wide Range ◽  
Genotype Diversity ◽  
Field Emergence

Abstract Proper storage of peanut (Arachis hypogaea L.) pods following drying is a critical step in maintenance of seed quality for the next planting season. The objective of this study was to examine the effect of ambient storage temperature (similar to farmers stock peanuts stored in warehouses) on seed germination and seedling vigor of selected peanut genotypes. Peanuts were grown in the field for three successive seasons. Pods were harvested, dried, and shelled. Seed samples were taken for storage under ambient conditions, humidity effects were eliminated by storing the seeds in plastic freezer bags in sealed containers. Seasons were: (1) 1986, 19 months storage at ambient temperature, (2) 1987, 7 months storage at ambient temperature, and (3) 1988, seeds without storage. When comparisons were made among genotypes within seasons, exposure to Season 1 conditions resulted in the least germination and seedling growth, but a wide range of genotype diversity occurred. Germination of seeds from Seasons 2 and 3 ranged from 81 to 98%, and significant differences in seedling growth occurred among genotypes. When comparisons were made across Seasons, the data indicated a significant storage effect, which resulted in different seed quality for individual genotypes. Usually field emergence was significantly different among genotypes and was highly correlated with germination for all seasons. Generally, emergence was negatively correlated with slow seedling growth and positively correlated with rapid seedling growth. Thus, for seeds of lower vigor (Seasons 1 and 2), rapid seedling growth was particularly critical for early, uniform emergence (10 DAP) in the field. Genotypes were significantly different in extent of seed quality reduction and field emergence both within and across storage periods. Genotype diversity to ambient storage conditions suggests there is genetic potential to improve longevity of seed quality during storage and enhance stability of field emergence.

scholarly journals The Effect of Various Film Packaging, Wax Coating and Storage Conditions on the Shelf Life and Quality of Pomegranate Fruits

2019 ◽  
Vol 27 (2) ◽  
pp. 47-54
Author(s):  
Mohammad Reza Safizadeh
Keyword(s):  
Shelf Life ◽  
Titratable Acidity ◽  
Punica Granatum ◽  
Storage Conditions ◽  
Ambient Condition ◽  
Ambient Conditions ◽  
Cold Condition ◽  
Wax Coating ◽  
And Control

AbstractIn this study, ‘Rabbab’ pomegranates (Punica granatum L.) were subjected before storage to single-layered wax coating (SLW), double-layered wax coating (DLW), individual-seal film packaging (ISP), tray wrap film packaging (TWP), and combined treatments of SLW + ISP or SLW + TWP. Treated fruits were then stored at ambient temperature (15–20 °C; 45–50% relative humidity – RH) or in cold conditions (5 °C ± 0.5; 85% RH) for 18 weeks. TWP, ISP, DLW, and SLW extended the shelf life of pomegranates for 18, 18, 12, and 11 weeks at cold (5 °C) condition, and also for 6, 5, 3, and 3 weeks at ambient condition, respectively, whereas the shelf life of control fruits were 10 and 2 weeks at cold and ambient conditions, respectively. After 18 weeks of storage, the weight loss in ISP and TWP fruits was 0.6 and 0.4% at cold condition and 12.4 and 5.4% at ambient condition, respectively. In general, film packaging maintained vitamin C, total titratable acidity, and sensory analysis scores for color, freshness, juiciness, and taste of pomegranates more effectively than wax coating and control. However, the combination of SLW and ISP or TWP did not improve the efficiency of pomegranates packaged as either ISP or TWP.

scholarly journals Quality of maize seeds harvested and husked at high moisture levels

2013 ◽  
Vol 35 (3) ◽  
pp. 276-283 ◽  
Author(s):  
Valquíria de Fátima Ferreira ◽  
João Almir Oliveira ◽  
Thaís Francielle Ferreira ◽  
Leandro Vilela Reis ◽  
Vanessa de Andrade ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seedling Emergence ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Cold Test ◽  
High Moisture ◽  
Maize Seeds ◽  
Seed Moisture ◽  
Seed Health Test

Harvest of maize seeds with high moisture levels has been recommended; nevertheless, after harvest, the ears undergo the process of husking and there is a need to assess the compatibility of these processes. The purpose of this study was to assess the effects of mechanical husking on the quality of maize seeds collected at high moisture levels. Dent BM 3061 hybrid maize seeds, manually collected from the ear, with moisture contents of 45%, 40% and 35%, and a 20/64 round sieve were used. The ears were mechanically husked (CWA machine, 312 rpm), dried in a stationary drier at 35 ⁰C until reaching 22% moisture, followed by drying at 42 ºC until reaching 12%. Then, they underwent shelling, followed by a chemical treatment with Maxin® + K-obiol® + Actellic®. Seed quality was assessed according to the occurrence tests of mechanical damage, first count, germination, seedling emergence, emergence speed index, average emergence time, electrical conductivity, accelerated aging, cold test, and seed health test at every quarter for 9 months. The mechanical husking affects maize seed quality, depending on the degree of seed moisture at harvest and on the material studied. This hybrid's seeds can be collected with seed moisture levels at 40% with the use of mechanical husking.

scholarly journals Effects Of Storage Conditions On The Quality Of Unripe Hazelnuts In The Husk

2015 ◽  
Vol 23 (2) ◽  
pp. 59-67 ◽  
Author(s):  
Bogumił Markuszewski ◽  
Jan Kopytowski
Keyword(s):  
Quality Assessment ◽  
Water Content ◽  
Dry Matter ◽  
Storage Conditions ◽  
Matter Content ◽  
Dry Matter Content ◽  
Controlled Atmosphere ◽  
Storage Diseases ◽  
Controlled Atmospheres

Abstract The purpose of the study was to assess the storability of unripe hazelnuts in the husk of four cultivars: ‘Hall’s Giant’, ‘Catalan’, ‘Webb’s Prize Cob’ and ‘Cosford’. The nuts were stored in normal and controlled atmospheres, and in Xtend® bags for three months. A quality assessment was performed based on the following parameters: weight of the nut in the husk and without the husk, weight of the kernel, percentage of nuts with husk attached, dry matter content in kernels, infection with fungal diseases, and the presence of physiological disorders. The study demonstrated that hazelnuts stored in Xtend® bags and under a controlled atmosphere had a higher weight for the nut in the husk and without the husk, as well as a higher weight of the kernel and water content when compared to batches of hazelnuts stored in a normal atmosphere. The percentage of nuts remaining in the husk was also higher when stored under such conditions. For the majority of investigated cultivars the storage in Xtend® bags, and to a lesser extent under normal atmosphere conditions, resulted in a substantial increase in nuts infected with fungal and abiotic diseases. Among investigated cultivars, ‘Hall’s Giant’ turned out to be the most resistant to storage diseases.

scholarly journals Seed quality of Amburana cearensis (Allemão) A.C. Sm. (Fabaceae) is influenced by storage condition

2017 ◽  
Vol 39 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Marcelo do Nascimento Araujo ◽  
Marisol Ferraz ◽  
Fabiana Karla Araújo Américo ◽  
Fabrício Francisco Santos Silva ◽  
Bárbara França Dantas ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Reducing Sugars ◽  
Seed Quality ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Soluble Sugars ◽  
Storage Condition ◽  
Storage Conditions ◽  
Total Soluble Sugars

Abstract: The aim of this study was to evaluate the effects of storage conditions on the germination of A. cearensis seeds. The experimental design was completely randomized into split plots over time with four replicates. The storage conditions of the airtight containers in the refrigerator and laboratory, paper bags in the laboratory and liquid nitrogen were assessed for 27 months. In the laboratory, we evaluated the germination, the germination rate, uniformity of germination, and total soluble and reducing sugars in the radicle. In the greenhouse, we evaluated seedling emergence, emergence rate and height of 30-day-old seedlings. Seeds stored in the refrigerator maintained a high initial germination rate, which decreased from the 21st month. Seeds stored in paper bags in the laboratory showed low emergence and small seedlings. Total soluble sugars and reducing sugars were mobilized when the seeds were stored at low temperatures. Thus, it is not advisable to store A. cearensis seeds in a laboratory environment without airtight containers. A. cearensis seeds kept in a refrigerated environment maintained their viability for at least two years.

Seed quality as cause for differences in longevity behaviour after seed pretreatment in wheat (Triticum aestivumL.)

1994 ◽  
Vol 4 (3) ◽  
pp. 323-328 ◽  
Author(s):  
P. Hofmann ◽  
A. M. Steiner
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
The Other ◽  
High Quality ◽  
Viability Loss ◽  
Seed Deterioration ◽  
Seed Pretreatment ◽  
Subsequent Storage ◽  
Wheat Seeds

AbstractHydration-dehydration seed pretreatments are well-known to improve germination rate, germination and vigour. However, contradictory results have been reported concerning the effect of a pretreatment on longevity. The present paper shows that the effect of a seed pretreatment on longevity is largely dependent on the quality of the seed at the time of pretreatment. Pretreating wheat seeds of high quality increased the rate of viability loss during subsequent storage. On the other hand, pretreating wheat seeds of relatively low quality decreased the rate of seed deterioration during subsequent storage. In the light of these findings, hitherto contradictory results in the literature could be explained.

Sign in / Sign up

Export Citation Format

Share Document