scholarly journals Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals

2009 ◽  
Vol 81 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Paulo M.P. Ferreira ◽  
Ana F.U. Carvalho ◽  
Davi F. Farias ◽  
Nara G. Cariolano ◽  
Vânia M.M. Melo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Moringa Oleifera ◽  
Biological Effects ◽  
Water Extract ◽  
Laboratory Animals ◽  
Egg Hatching ◽  
Lc50 Value ◽  
Low Toxicity ◽  
Toxic Potential

In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS) were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats). Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF) and nondyalizable (NDF) fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL) and mice (LD50 of 446.5 mg/kg body weight) pointed out to low toxicity. Despite the thymus hypertrophy, WEMOS revealed to be harmless in orally and subacutelytreated rats. In conclusion, WEMOS has thermostable bioactive compounds against Ae. aegypti larvae with apparent molecular mass lower than 12 kDa and moderately toxic potential.

Exposure of mosquito (Aedes aegypti) larvae to the water extract and lectin-rich fraction of Moringa oleifera seeds impairs their development and future fecundity

2019 ◽  
Vol 183 ◽  
pp. 109583 ◽  
Author(s):  
Livia Lais de Santana Silva ◽  
Kenner Morais Fernandes ◽  
Franciane Rosa Miranda ◽  
Stella Cristina Cabral Silva ◽  
Luana Cassandra Breitenbach Barroso Coelho ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Moringa Oleifera ◽  
Water Extract

scholarly journals Effects of a solid formulation containing lectin-rich fraction of Moringa oleifera seeds on egg hatching and development of Aedes aegypti larvae

Acta Tropica ◽  
2021 ◽  
Vol 214 ◽  
pp. 105789
Author(s):  
Livia Lais de Santana Silva ◽  
Stella Cristina Cabral Silva ◽  
Ana Patrícia Silva de Oliveira ◽  
Jéssica da Silva Nascimento ◽  
Emerson de Oliveira Silva ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Moringa Oleifera ◽  
Egg Hatching

scholarly journals Larvicidal Activity in vivo of Ethanolic and Aqueous Extracts From Moringa (Moringa oleifera Lam.) on Aedes aegypti L. (Diptera: Culicidae)

2019 ◽  
Vol 11 (8) ◽  
pp. 129
Author(s):  
Antonio Carlos Leite Alves ◽  
Toshik Iarley da Silva ◽  
Francisco Roberto de Azevedo ◽  
Estelita Pereira Lima ◽  
Renata Rocha Virgulino ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Control Treatment ◽  
Control Group ◽  
Distilled Water ◽  
Aqueous Extracts ◽  
Extract Concentration

The insecticides properties of Moringa oleifera (moringa) were evaluated in Aedes aegypti larvae, throughout an entirely randomized trial, represented by ethanolic and aqueous extracts obtained from leaves, flowers, barks, seeds and moringa roots, besides the control group. Five batches of 10 in 3rd stadium larvae were distributed in distilled water, added an extract concentration of 50 mL L-1, and the control treatment (distilled water). The test reading was measured after 24, 48 and 72 hours after larvae exposure, were considered dead those who did not respond to a mechanical stimulation of a clamp. The seed ethanolic extract produced the best performance after 24 hours (34% mortality), but after 48 hours, the flower extract was the more potent (38% mortality). The largest larvicidal activity was observed with the extract concentration of 90 mL L-1. The results indicate that moringa has larvacides properties against Aedes, but its chemical constituents need to be isolated and tested separately to enhance your larvicidal activity.

scholarly journals Aktivitas Larvasida Berbagai Pelarut pada Ekstrak Biji Kayu Besi Pantai (Pongamia pinnata) terhadap Mortalitas Larva Aedes spp.

2018 ◽  
pp. 125-132
Author(s):  
Yuneu Yuliasih ◽  
Mutiara Widawati
Keyword(s):  
Larvicidal Activity ◽  
Harmful Effect ◽  
Water Extract ◽  
Chloroform Extract ◽  
Pongamia Pinnata ◽  
Control Group ◽  
Control Group Design ◽  
Lc50 Value ◽  
Materials Used ◽  
Seed Extracts

The use of chemical compound as a way to control DHF may cause harmful effect for humans and environment. It is necessary to develop biological insecticides derived from plants such as Pongamia pinnata. This study was designed to determine the larvicidal activity of  P. pinnata seeds extract with various solvent on  Aedes spp. This study was an experimental research with post test only control group design. Materials used in the research were P. pinnata seed extracts with water, methanol and chloroform solvents of concentration; 100 ppm, 200 ppm, 300 ppm, 400 ppm and 500 ppm. Larvicidal activity of P. pinnata seed extracts was tested against third instar larvae of Ae. aegypti and Ae. albopictus, 24 hours observation was carried out within each treatment.very test was repeated for 3 times. The result showed that larvae mortality was found in the methanol extract with LC50 value of 141.88 ppm for Ae. aegypti and 108.19 for Ae. albopictus, and in the chloroform extract with LC50 value of 346.06 ppm for Ae. aegypti and 222.29 ppm for Ae. albopictus. The water extract of  P. pinnata seed was not effective as a larvicidal, while methanol and chloroform of P. pinnata seed extracts act as potential natural insecticide.

scholarly journals Momordica charantia L. extracts against Aedes aegypti larvae

2022 ◽  
Vol 82 ◽  
Author(s):  
L. M. P. Mituiassu ◽  
M. T. Serdeiro ◽  
R. R. B. T. Vieira ◽  
L. S. Oliveira ◽  
M. Maleck
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Methanol Extract ◽  
Larval Mortality ◽  
High Toxicity ◽  
The Third ◽  
Low Toxicity ◽  
Aegypti Population ◽  
Ethyl Acetate Extracts

Abstract Mormodica charantia (Curcubitaceae) is a plant with great medicinal potential, also used as an alternative of mosquitoes control as demonstrated by previous studies. We evaluated the larvicidal activity of crude extracts of ethyl acetate, methanol and hexane from flowers and fruits of M. charantia against Aedes aegypti (Culicidae). Flowers and fruits were macerated in methanol, ethyl acetate and hexane. Bioassays were performed with application of the extracts at final concentrations of 1 - 200 µg/mL in the middle of the third instar larvae of A. aegypti (L3). The results showed high toxicity to ethyl acetate extracts from flowers and fruits at concentrations of 200 µg/mL and 100 µg/mL, with 97% and 87% of larvae mortality (L3), respectively. Hexane extract demonstrated low toxicity, while methanol extract exhibited 78% larval mortality. The data suggested that the ethyl acetate extracts of flowers and fruits of M. charantia can effectively contribute to larvicidal activity. In addition, purification of M. charantia extracts may lead to a promising larvicidal activity to control the A. aegypti population.

scholarly journals Screening of some weed extracts for ovicidal and larvicidal activities against dengue vector Aedes aegypti

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Sarika Torawane ◽  
Ramnath Andhale ◽  
Radhakrishna Pandit ◽  
Digambar Mokat ◽  
Samadhan Phuge
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Plant Extracts ◽  
Insect Pests ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Egg Hatching ◽  
Ms Analysis ◽  
Ethanol Extracts ◽  
Larvicidal Activities

Abstract Background The use of plant-based formulations is an easy and economical method to control insect pests. The objectives of the present study were to evaluate ovicidal and larvicidal potential of ethanolic leaf extracts of four plants, Cyathocline purpurea, Blumea lacera, Neanotis lancifolia, and Neanotis montholonii, and their effects on gut histology of Aedes aegypti (Diptera: Culicidae) larvae. We identified the phytochemicals present in the ethanolic extracts of these plants by LC-MS analysis. The eggs and larvae of A. aegypti were exposed to four concentrations (0.05, 0.1, 0.2, and 0.3 mg/mL) of crude ethanol extracts for different time durations. We observed egg hatching for 48 h, mortality, and gut histology of the 3rd instar larvae after 24, 48, and 72 h of treatments. Results LC-MS analysis revealed the presence of various active compounds such as alkaloids, tannins, saponins, and flavonoids in the ethanol extracts of all these plants. The extracts of all plants showed ovicidal activity. The extracts of C. purpurea showed the highest larvicidal activity (LC50 at 72 h = 0.088 mg/mL) followed by B. lacera (LC50 at 72 h = 0.758 mg/mL) while N. lancifolia and N. montholonii extracts did not show significant larvicidal activity. All plant extracts affected gut morphology in concentration and treatment duration-dependent manner. The plant extracts used in the present study have ovicidal properties and affected the gut histology of A. aegypti larvae. Conclusion These results suggest that C. purpurea has the highest larvicidal activity against A. aegypti larvae and can be further evaluated for application purposes.

A Simple and Efficient Protocol for the Knoevenagel Reaction of Benzylidenemalononitriles and the Evaluation of the Larvicidal Activity on Aedes Aegypti

2018 ◽  
pp. 362-374
Author(s):  
Harlyson L. Carvalho ◽  
Andréia L. de Amorim ◽  
Inana F. Araújo ◽  
Bianca L. B. Marino ◽  
David E. Q. Jimenez ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Knoevenagel Reaction

scholarly journals Naphthalimide-Containing BP100 Leads to Higher Model Membranes Interactions and Antimicrobial Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 542
Author(s):  
Gustavo Penteado Battesini Carretero ◽  
Greice Kelle Viegas Saraiva ◽  
Magali Aparecida Rodrigues ◽  
Sumika Kiyota ◽  
Marcelo Porto Bemquerer ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Biological Activities ◽  
Biological Effects ◽  
Membrane Surface ◽  
Helical Structure ◽  
Helical Conformation ◽  
Property A ◽  
Cellular Processes ◽  
Double Stranded Dna ◽  
Low Toxicity

In a large variety of organisms, antimicrobial peptides (AMPs) are primary defenses against pathogens. BP100 (KKLFKKILKYL-NH2), a short, synthetic, cationic AMP, is active against bacteria and displays low toxicity towards eukaryotic cells. BP100 acquires a α-helical conformation upon interaction with membranes and increases membrane permeability. Despite the volume of information available, the action mechanism of BP100, the selectivity of its biological effects, and possible applications are far from consensual. Our group synthesized a fluorescent BP100 analogue containing naphthalimide linked to its N-terminal end, NAPHT-BP100 (Naphthalimide-AAKKLFKKILKYL-NH2). The fluorescence properties of naphthalimides, especially their spectral sensitivity to microenvironment changes, are well established, and their biological activities against transformed cells and bacteria are known. Naphthalimide derived compounds are known to interact with DNA disturbing related processes as replication and transcription, and used as anticancer agents due to this property. A wide variety of techniques were used to demonstrate that NAPHT-BP100 bound to and permeabilized zwitterionic POPC and negatively charged POPC:POPG liposomes and, upon interaction, acquired a α-helical structure. Membrane surface high peptide/lipid ratios triggered complete permeabilization of the liposomes in a detergent-like manner. Membrane disruption was driven by charge neutralization, lipid aggregation, and bilayer destabilization. NAPHT-BP100 also interacted with double-stranded DNA, indicating that this peptide could also affect other cellular processes besides causing membrane destabilization. NAPHT-BP100 showed increased antibacterial and hemolytic activities, compared to BP100, and may constitute an efficient antimicrobial agent for dermatological use. By conjugating BP100 and naphthalimide DNA binding properties, NAPHT-BP100 bound to a large extent to the bacterial membrane and could more efficiently destabilize it. We also speculate that peptide could enter the bacteria cell and interact with its DNA in the cytoplasm.

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