Preserved xenogenic amniotic membrane as a patch on the repair of superficial corneal ulcers in rabbits

The aim of this study was to evaluate the effects of canine amniotic membrane, previously preserved in glycerin, used as a patch on the repair of experimentally-made superficial corneal ulcers and to compare corneal epithelization between the treated and non-treated groups. Xenogeneic amniotic membranes were collected aseptically and preserved in 99% glycerin at room temperature. Each animal was anesthetized and submitted to superficial corneal keratectomy of the left eye. The treated group received a fragment of canine amniotic membrane as a patch, while the control group had no treatment. The treated group showed blepharospasm, ocular discharge and conjunctival congestion. The membrane accelerated corneal repair in the beginning of the process, however, it delayed its conclusion (p<0.05). Treated eyes showed greater vessel formation and decreased corneal transparency (p<0.05). The stroma of the control group was thicker than that of the treated group (p<0.05). We suggest that amniotic membrane used in this manner can be applied as a therapy for superficial corneal ulcers in the beginning phases of the repair process.

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Amniotic membrane applied to burns healing: Pre-clinical study

Research Society and Development ◽

10.33448/rsd-v10i4.14286 ◽

2021 ◽

Vol 10 (4) ◽

pp. 38110414286

Author(s):

Fernanda Cláudia Miranda Amorim ◽

Emilia Ângela Loschiavo Arisawa ◽

Luciana Barros Sant’Anna ◽

Khetyma Moreira Fonseca ◽

Davidson Ribeiro Costa ◽

...

Keyword(s):

Amniotic Membrane ◽

Healing Process ◽

Inflammatory Cells ◽

Repair Process ◽

Treated Group ◽

Preclinical Study ◽

Control Group ◽

Human Amniotic Membrane ◽

Significance Level ◽

Kolmogorov Smirnov

This preclinical study aimed to evaluate the tissue repair process of burns treated with human amniotic membrane (hAM) patches in rats. Twenty-four rats were subjected to superficial burns of partial thickness, and randomly allocated into two groups: Control and Treated Group, subdivided into two experimental periods of 7th and 14th days. The lesions were evaluated by digitalized images (macroscopy) and by the analysis of histological sections stained in H&E to quantify the number of inflammatory cells and fibroblasts present in the different experimental times (histomorphometry). The histomorphometric analyses were performed blindly. Statistical analysis employed Kolmogorov-Smirnov and Mann Whitney tests, with 95% confidence interval at 5% significance level (p <0.05). Macroscopically, the lesions of Treated group presented a crust formation before Control Group, and there were no signs of infection in both groups. Microscopically, the qualitative analysis showed a faster evolution in the healing process of the Treated groups compared to the Control, with reduction of the inflammatory infiltrate, intense fibroblasts proliferation and better organization of the collagen fibers. The quantitative analysis showed statistically significant results regarding the reduction of inflammatory cells (p<0.0001) at 7th and 14 th day and increased proliferation of fibroblasts at 14th day (p<0.0001) in lesions treated with hAM compared to Control group. The results of this preclinical study demonstrated that the application of hAM patches reduces the inflammatory process and accelerates the onset of the proliferative phase in burn injuries.

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REPARAÇÃO DO TENDÃO CALCÂNEO EM RATOS APÓS TENOTOMIA E TRATAMENTO POR ABELMOSCHUS ESCULENTUS LINN E ULTRASSOM TERAPÊUTICO

Revista Interdisciplinar de Estudos em Saúde ◽

10.33362/ries.v8i1.1448 ◽

2019 ◽

Vol 8 (1) ◽

pp. 41-55

Author(s):

Gabriel Martins de Barros ◽

Monaliza Sousa dos Anjos ◽

Antônio Luiz Martins Maia Filho ◽

Wellington Dos Santos Alves ◽

Valdiléia Teixeira Uchôa ◽

...

Keyword(s):

Tendon Repair ◽

Repair Process ◽

Treated Group ◽

Abelmoschus Esculentus ◽

Control Group ◽

Adjuvant Effect ◽

Calcaneal Tendon ◽

Significance Level ◽

Male Wistar Rats ◽

One Way Anova

Este estudo objetivou comparar o efeito da reparação tendínea por Abelmoschus esculentus Linn (AEL) e ultrassom terapêutico (US) em modelo experimental de tenotomia. Foram selecionados 40 ratos machos Wistar que passaram por uma tenotomia na pata direita sendo tratados durante 7 e 14 dias consecutivos, os animais foram divididos em grupos com tratamento de AEL por via tópica, US, conjunto das duas terapias, além do grupo controle. Após o tratamento os tendões foram removidos para análise histológica, sendo realizado a contagem de células inflamatórias e fibroblásticas. A análise estatística foi feita através da variação do número de células no respectivo grupo, utilizando a análise de One Way ANOVA seguido pelo teste de Newman-keuls com nível de significância de 5% (p0,05). Nos resultados foi visto a redução significativa (p0,05) de células polimorfonucleadas do grupo tratado com US + AEL em relação ao grupo controle no tempo de 14 dias. Em relação ao número de células fibroblásticas houve aumento significativo nos grupos tratados com sonoforese em relação ao grupo controle em 7 e 14 dias. Nos outros grupos não houve diferenças significativas. A terapia com US e AEL mostrou efeito adjuvante no processo de reparo do tendão, demostrando eficácia da sonoforese com AEL.Palavras-chave: Ratos Wistar. Tendinite. Quiabo. Terapia por Ultrassom. Fonoforese. REPAIR OF THE CALCANEAL TENDON IN RATS AFTER TENOTOMY AND TREATMENT BY ABELMOSCHUS ESCULENTUS LINN AND THERAPEUTIC ULTRASOUNDABSTRACT: This study aimed to compare the effect of tendon repair by Abelmoschus esculentus Linn (AEL) and therapeutic ultrasound (US) in an experimental model of tenotomy. A total of 40 male Wistar rats were submitted to a tenotomy in the right paw and were treated for 7 and 14 consecutive days. The animals were divided into groups with AEL treatment topical, US, all of the therapies, as well as the control group. After the treatment, the tendons were removed for histological analysis, counting inflammatory and fibroblast cells. Statistical analysis was done by varying the number of cells in the respective group, using One Way ANOVA followed by Newman-keuls test with significance level of 5% (p0.05). The results showed a significant reduction (p 0.05) in polymorphonucleated cells from the US + AEL treated group compared to the 14-day control group. In relation to the number of fibroblastic cells there was a significant increase in the groups treated with phonophoresis in relation to the control group in 7 and 14 days. There were no significant differences in the other groups. US and AEL therapy showed an adjuvant effect on the tendon repair process, demonstrating the efficacy of phonophoresis with AEL.Keywords: RatsWistar. Tendinopathy. Abelmoschus. Ultrasonic Therapy. Phonophoresis.

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Avaliação do Efeito da Microcorrente na Reparação de Feridas Cutâneas em Ratos

UNICIÊNCIAS ◽

10.17921/1415-5141.2018v22n2p62-65 ◽

2018 ◽

Vol 22 (2) ◽

pp. 62

Author(s):

Belle Virginia Da Silva Coimbra Rodrigues ◽

Suellen Borges de Souza ◽

Thayanne Caroline Pereira Munhoz ◽

Gracieli Nonato Bressanin ◽

Alessandra Aparecida Cunha Lopes ◽

...

Keyword(s):

Tissue Repair ◽

Specific Treatment ◽

Low Frequency ◽

Repair Process ◽

Skin Lesions ◽

Treated Group ◽

Therapeutic Modality ◽

Cervical Region ◽

Control Group ◽

And Control

A bioeletricidade tem se tornado cada vez mais reconhecida no mundo científico devido a sua importante contribuição na cicatrização de feridas. Dentro desta modalidade terapêutica está inserida a terapia com microcorrente, que por ter baixa frequência e pequena intensidade é capaz de promover o restabelecimento do potencial elétrico das células. Por se tratar de uma corrente de elevado potencial, durante o processo de reparação tecidual, esta pode atuar tanto na fase inflamatória quanto na proliferativa. Dessa maneira, este estudo teve por objetivo principal avaliar a resposta cicatricial quanto ao efeito da microcorrente em lesões cutâneas em ratos. Para tanto, foram utilizados 28 ratos Wistar, divididos em dois grupos: grupo tratado (GT) e grupo controle (GC). Os animais de ambos os grupos foram submetidos a procedimento cirúrgico de lesão cutânea região cervical dorsal, seguido de tratamento com microcorrente durante 21 dias no GT, e sem tratamento específico no GC. Também foi realizada a avaliação termográfica a cada três dias de todos os animais. Após este período, foi feita eutanásia com posterior coleta de pele na região da lesão para avaliação histopatológica. Os resultados obtidos mostraram, a partir dos parâmetros analisados, a presença de um recurso capaz de incrementar a formação de novos vasos, embora não apresentasse efeito significativo em relação à proliferação de fibroblastos. Diante disso, sugere-se que a utilização da microcorrente possa ter favorecido a neovascularização nos tecidos dos animais analisados. Palavras-chave: Rato. Microcorrente. Angiogênese. Reparo tecidual. AbstractBioelectricity has become increasingly recognized in the scientific world because of its important contribution to wound healing. Within this therapeutic modality the therapy with microcurrent is inserted, which because of low frequency and small intensity is able to promote the reestablishment of the cells electric potential. Because it is a high potential v during the tissue repair process, it can act both in the inflammatory and proliferative phases. Thus, this study had as main objective to evaluate the healing response regarding the microcurrent effect on rats’ skin lesions. For this, 28 Wistar rats were divided into two groups: treated group (GT) and control group (CG). The animals of both groups underwent a surgical procedure of skin lesion in the dorsal cervical region, followed by treatment with microcurrent for 21 days in the GT, and without specific treatment in the CG. A thermographic evaluation was also performed every three days of all the animals. After this period, euthanasia was performed with subsequent skin collection in the lesion region for histopathological evaluation. The obtained results showed, from the analyzed parameters, the presence of a resource able to increase the new vessels formation, although it did not present significant effect in relation to the fibroblasts proliferation. Therefore, it is suggested that the use of microcurrent may have favored neovascularization in the tissues of the animals analyzed.Keywords: Mouse. Microcurrent. Angiogenesis. Tissue repair.

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Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-7092 ◽

2015 ◽

Vol 67 (2) ◽

pp. 325-333

Author(s):

R.B. Eleotério ◽

K.C.S. Pontes ◽

J.P. Machado ◽

E.C.C. Reis ◽

P.S. Ferreira ◽

...

Keyword(s):

Articular Cartilage ◽

Subchondral Bone ◽

Bone Repair ◽

Blood Clot ◽

Femoral Condyle ◽

Repair Process ◽

Treated Group ◽

Glucosamine Sulfate ◽

Control Group ◽

Sample Collection

Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.

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Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652012000300026 ◽

2012 ◽

Vol 84 (3) ◽

pp. 841-851 ◽

Cited By ~ 9

Author(s):

Betânia S. Monteiro ◽

Napoleão M. Argôlo-Neto ◽

Nance B. Nardi ◽

Pedro C. Chagastelles ◽

Pablo H. Carvalho ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Bone Repair ◽

Bone Matrix ◽

Cell Types ◽

Repair Process ◽

Treated Group ◽

Control Group ◽

Calvarial Bone

Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp+ were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that observed in the control group. The results demonstrated that transplantation of bone marrow-derived MSC of C57BL/6 gfp+ mice to bone critical defects produced in mice calvarial contributes positively to the bone repair process. MSC presets ability to influence the correct functioning of osteoblasts, increases the amount of mobilized cells for the repairing process, speeds up growth, and increases deposition of bone matrix.

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The Effectivenes of Red Rice to Decrease Total Cholesterol in Type 2 DM Patients

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v3i1.4091 ◽

2017 ◽

Vol 3 (1) ◽

pp. 7

Author(s):

Wydi Ulfa Pradini ◽

Ancah Caesarina Novi Marchianti ◽

Rini Riyanti

Keyword(s):

Diabetes Mellitus ◽

Total Cholesterol ◽

Treated Group ◽

Control Group ◽

Clinical Test ◽

Red Rice ◽

Cholesterol Levels ◽

Type 2 Dm ◽

In The Beginning

Diabetes mellitus (DM) is a group of metabolic diseases with hyperglycemia characteristic that happens because anomaly of insulin secretion or insulin activity. Insulin deficiency in type 2 DM caused dislipidemia. Red rice are contain fiber, essential fatty acid and Gamma Amino Butiric Acid (GABA). This research intended to know the effectiveness of red rice to decrease total cholesterol levels of patient with type 2 DM. Clinical test with quasi experimental and research design non randomized control-group pretest-postest design was used for the research method. Total samples of this research are 36 samples. The treated group was given the red rice during 6 days on breakfast and dinner, while the control group did not have any intervention. The research showed that average cholesterol levels in the beginning and finale of treated group was 235,69 mg/dL and 198,56 mg/dL, while average cholesterol levels in the beginning and finale of control group was 235,72 mg/dL and 256,50 mg/dL. From this research, red rice has effective to decrease total cholesterol levels of patients with type 2 DM.Keywords: Diabetes mellitus, Red Rice, Cholesterol total levels

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Hydroxytyrosol effectively improves the quality of pig sperm at room temperature

10.21203/rs.3.rs-61554/v1 ◽

2020 ◽

Author(s):

Wenuyu Zhang ◽

Dong Li ◽

Xueka Tian ◽

Yulin He ◽

Zitong Xiao ◽

...

Keyword(s):

Artificial Insemination ◽

Room Temperature ◽

Sperm Quality ◽

Membrane Damage ◽

Treated Group ◽

Control Group ◽

Sequencing Analysis ◽

Oxygen Species ◽

Boar Semen

Abstract Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. Preservation of semen is mainly conducted at room temperature (17°C), during which the accumulation of reactive oxygen species (ROS). In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of boar semen at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, Proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol (optimum concentration) group after three days’ storage. Ultimately, we selected the control group and the addition of 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: During storage of pig semen at room temperature, the sperm quality of the hydroxytyrosol-treated group was significantly improved (P < 0.05). Besides, its antioxidant capacity was also significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm cell quality. The significant increase in sow pregnancy rate and the increase in piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent can not only improve the quality of pig sperm, but also has an irreplaceable role in improving the efficiency of artificial insemination.

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Comparative evaluation of bovine pericardial membrane and amniotic membrane in wounds skin healing in rabbits

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v41i2.63 ◽

2018 ◽

Vol 41 (2) ◽

pp. 137-145

Author(s):

N. H. Al-Falahi

Keyword(s):

Amniotic Membrane ◽

Skin Wound ◽

Application Site ◽

Control Group ◽

Local Breed ◽

Inflammatory Phase ◽

Thickness Skin ◽

Skin Healing ◽

Amniotic Membranes ◽

And Control

The present study aimed to compare between the amniotic and pericardial membranes for the wound dressing models. Ten local breed rabbits have been used in the current study. They were divided into two main equal groups: Experiment (Amniotic and pericardial membranes) and control group. According to healing periods each group was subdivided into four subgroups (7, 14, 21 and 28) days post wounding. Specimens of amniotic membranes were collected from full term pregnant cows, and pericardium was obtained from abattoir immediately after slaughtering. The study was conducted on skin wound for experiment application site as full-thickness skin wounds (3×3 cm) were done on the dorsal thoracic sides. Histopathological evaluation of study depended on the degrees of inflammatory exudate, epithelialization, fibroplasia and type of leukocytes infiltration. Results revealed marked reduction of the inflammatory phase during all periods of post treatment with amniotic membrane, the degree of epithelization and fibroplasia in addition to angiogenesis were enhanced in amniotic and pericardial dressed wounds during period of 14 and 21 days. The study concluded that no significant differences are between the pericardial membrane and of amniotic membrane in wound healing of skin.

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Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.76.1.28 ◽

2006 ◽

Vol 76 (1) ◽

pp. 28-33 ◽

Cited By ~ 7

Author(s):

Yukari Egashira ◽

Shin Nagaki ◽

Hiroo Sanada

Keyword(s):

Body Weight ◽

Urinary Excretion ◽

Enzyme Activities ◽

Treated Group ◽

Control Group ◽

Puromycin Aminonucleoside ◽

Low Level ◽

Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

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Haemostatic Clot Formation at Anastomosis of Synthetic Venous Graft in Defibrinogenated Dogs: A Scanning Electron Microscopic Study

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650187 ◽

1981 ◽

Vol 45 (03) ◽

pp. 276-281 ◽

Cited By ~ 2

Author(s):

S Ishimaru ◽

E Berglin ◽

H-A Hansson ◽

A-C Teger-Nilsson ◽

G William-Olsson

Keyword(s):

Vena Cava ◽

Treated Group ◽

Control Group ◽

Electron Microscopic ◽

Scanning Electron Microscopic Study ◽

Fibrin Network ◽

Scanning Electron Microscopic ◽

Expanded Polytetrafluoroethylene Graft ◽

Morphological Differences ◽

Scanning Electron

SummaryA segment of the inferior vena cava was replaced by an expanded polytetrafluoroethylene graft in 13 dogs. Five of them served as a control group, while the other 8 were moderately or severely defibrinogenated with subcutaneous batroxobin. Plasma fibrinogen decreased to extremely low values throughout the experiment in the defibrinogenated dogs except in the moderately treated group in which it temporarily rose to 0.72-0.87 g/1 on the first postoperative day.Scanning electron microscopic observations of the haemostatic clot formed at the anastomoses of the graft revealed no significant morphological differences in platelet adhesion and/or aggregation between the three groups. These findings confirmed that platelets play a key role in primary haemostasis during defibrinogenation.The fibrin network was slightly diminished and only short fibrin filaments could be seen in the moderately and severely defibrinogenated groups respectively. These differences in composition of the clots are discussed in relation to their haemostatic capacity.

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