Amniotic membrane applied to burns healing: Pre-clinical study

This preclinical study aimed to evaluate the tissue repair process of burns treated with human amniotic membrane (hAM) patches in rats. Twenty-four rats were subjected to superficial burns of partial thickness, and randomly allocated into two groups: Control and Treated Group, subdivided into two experimental periods of 7th and 14th days. The lesions were evaluated by digitalized images (macroscopy) and by the analysis of histological sections stained in H&E to quantify the number of inflammatory cells and fibroblasts present in the different experimental times (histomorphometry). The histomorphometric analyses were performed blindly. Statistical analysis employed Kolmogorov-Smirnov and Mann Whitney tests, with 95% confidence interval at 5% significance level (p <0.05). Macroscopically, the lesions of Treated group presented a crust formation before Control Group, and there were no signs of infection in both groups. Microscopically, the qualitative analysis showed a faster evolution in the healing process of the Treated groups compared to the Control, with reduction of the inflammatory infiltrate, intense fibroblasts proliferation and better organization of the collagen fibers. The quantitative analysis showed statistically significant results regarding the reduction of inflammatory cells (p<0.0001) at 7th and 14 th day and increased proliferation of fibroblasts at 14th day (p<0.0001) in lesions treated with hAM compared to Control group. The results of this preclinical study demonstrated that the application of hAM patches reduces the inflammatory process and accelerates the onset of the proliferative phase in burn injuries.

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REPARAÇÃO DO TENDÃO CALCÂNEO EM RATOS APÓS TENOTOMIA E TRATAMENTO POR ABELMOSCHUS ESCULENTUS LINN E ULTRASSOM TERAPÊUTICO

Revista Interdisciplinar de Estudos em Saúde ◽

10.33362/ries.v8i1.1448 ◽

2019 ◽

Vol 8 (1) ◽

pp. 41-55

Author(s):

Gabriel Martins de Barros ◽

Monaliza Sousa dos Anjos ◽

Antônio Luiz Martins Maia Filho ◽

Wellington Dos Santos Alves ◽

Valdiléia Teixeira Uchôa ◽

...

Keyword(s):

Tendon Repair ◽

Repair Process ◽

Treated Group ◽

Abelmoschus Esculentus ◽

Control Group ◽

Adjuvant Effect ◽

Calcaneal Tendon ◽

Significance Level ◽

Male Wistar Rats ◽

One Way Anova

Este estudo objetivou comparar o efeito da reparação tendínea por Abelmoschus esculentus Linn (AEL) e ultrassom terapêutico (US) em modelo experimental de tenotomia. Foram selecionados 40 ratos machos Wistar que passaram por uma tenotomia na pata direita sendo tratados durante 7 e 14 dias consecutivos, os animais foram divididos em grupos com tratamento de AEL por via tópica, US, conjunto das duas terapias, além do grupo controle. Após o tratamento os tendões foram removidos para análise histológica, sendo realizado a contagem de células inflamatórias e fibroblásticas. A análise estatística foi feita através da variação do número de células no respectivo grupo, utilizando a análise de One Way ANOVA seguido pelo teste de Newman-keuls com nível de significância de 5% (p0,05). Nos resultados foi visto a redução significativa (p0,05) de células polimorfonucleadas do grupo tratado com US + AEL em relação ao grupo controle no tempo de 14 dias. Em relação ao número de células fibroblásticas houve aumento significativo nos grupos tratados com sonoforese em relação ao grupo controle em 7 e 14 dias. Nos outros grupos não houve diferenças significativas. A terapia com US e AEL mostrou efeito adjuvante no processo de reparo do tendão, demostrando eficácia da sonoforese com AEL.Palavras-chave: Ratos Wistar. Tendinite. Quiabo. Terapia por Ultrassom. Fonoforese. REPAIR OF THE CALCANEAL TENDON IN RATS AFTER TENOTOMY AND TREATMENT BY ABELMOSCHUS ESCULENTUS LINN AND THERAPEUTIC ULTRASOUNDABSTRACT: This study aimed to compare the effect of tendon repair by Abelmoschus esculentus Linn (AEL) and therapeutic ultrasound (US) in an experimental model of tenotomy. A total of 40 male Wistar rats were submitted to a tenotomy in the right paw and were treated for 7 and 14 consecutive days. The animals were divided into groups with AEL treatment topical, US, all of the therapies, as well as the control group. After the treatment, the tendons were removed for histological analysis, counting inflammatory and fibroblast cells. Statistical analysis was done by varying the number of cells in the respective group, using One Way ANOVA followed by Newman-keuls test with significance level of 5% (p0.05). The results showed a significant reduction (p 0.05) in polymorphonucleated cells from the US + AEL treated group compared to the 14-day control group. In relation to the number of fibroblastic cells there was a significant increase in the groups treated with phonophoresis in relation to the control group in 7 and 14 days. There were no significant differences in the other groups. US and AEL therapy showed an adjuvant effect on the tendon repair process, demonstrating the efficacy of phonophoresis with AEL.Keywords: RatsWistar. Tendinopathy. Abelmoschus. Ultrasonic Therapy. Phonophoresis.

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A Novel Self-Assembled Gel for Gastric Endoscopic Submucosal Dissection-Induced Ulcer: A Preclinical Study in a Porcine Model

Frontiers in Pharmacology ◽

10.3389/fphar.2021.700387 ◽

2021 ◽

Vol 12 ◽

Author(s):

Meng Li ◽

Haifeng Jin ◽

Changpei Shi ◽

Bin Lyu ◽

Xiao Ying ◽

...

Keyword(s):

Endoscopic Submucosal Dissection ◽

Ulcer Healing ◽

Healing Process ◽

Treated Group ◽

Preclinical Study ◽

Gastric Ulcers ◽

Control Group ◽

Submucosal Dissection ◽

Randomized Control ◽

Self Assembled

Endoscopic submucosal dissection (ESD) is a routine procedure for the management of early gastrointestinal neoplasia, but it results in large ulcers. This study aims to examine the feasibility and effectiveness of a newly developed self-assembled gel on the ulcer healing process after ESD. Sixteen 2.0-cm (diameter) gastric ulcers were created by ESD in five pigs. All ulcers were randomized: control group (n = 7, routine ulcer management) and gel-treated group (n = 9). The gel was applied to cover the whole ulcer bed through the endoscope immediately after ESD. The feasibility of this endoscopic treatment modality was macroscopically accessed by endoscopy. The effectiveness was evaluated based on the ulcer area and histology changes at 14 days after ESD. The gel-treated group showed higher healing activity compared with the control ulcers on day 14 after ESD. The percentage of healing was higher for the gel-treated ulcers than in control ones (96.2 ± 2.2% vs. 91.9 ± 4.5%, p = 0.035). The extent of the new epithelium covering the ulcer was greater in the gel group than in controls. One delayed overt bleeding occurred in the control group (14.3%). This novel gel might promote the speed of ulcer healing after ESD, leading to higher epithelium formation.

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Experimental study of amniotic membrane or topical propolis on induced cutaneous wound healing in Wistar rats

Acta Veterinaria Brasilica ◽

10.21708/avb.2021.15.3.9991 ◽

2021 ◽

Vol 15 (3) ◽

pp. 253-260

Author(s):

Rafaela Eduarda dos Reis ◽

Letícia da Silva Rueda ◽

Bruno Roberto Vidal Tuani ◽

Cláudia Sampaio Fonseca Repetti ◽

Camila Dias Porto ◽

...

Keyword(s):

Amniotic Membrane ◽

Rattus Norvegicus ◽

Healing Process ◽

Inflammatory Cells ◽

Control Group ◽

Lesion Area ◽

Group Iii ◽

Group I ◽

Surgical Wounds

The study of propolis and the amniotic membrane as alternatives to promote the acceleration of healing has grown considerably. These products have shown to be easy to use, have bactericidal or bacteriostatic action and are affordable. The objective of the present study was to evaluate the action of the amniotic membrane of bitches and propolis in the healing of surgical wounds. To evaluate the efficacy of these methods the time for conclusion of the healing process of both groups was compared with a control group. In addition, the inflammatory response of each group was also evaluated to identify which alternative method could induce a better response in the healing process compared to the control group. For this purpose, 27 animals, Rattus norvegicus, variety Wistar, were used. Rats were separated into 3 groups with 9 animals each. Group I represented the 25% alcoholic propolis solution; Group II, Amniotic membrane; Group III was Control group. Wounds were assessed macroscopically for the lesion area, and microscopically for the histological quality of each wound layer and the intensity of inflammation and collagen deposition. After the macroscopic analysis, it was observed that there were no statistical differences among the 3 groups (p> 0.05) relevant to accelerate the healing process in terms of presence of inflammatory cells, fibroblasts, collagen, neovascularization, granulation tissue and re-epithelialization.

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Preserved xenogenic amniotic membrane as a patch on the repair of superficial corneal ulcers in rabbits

Revista CERES ◽

10.1590/s0034-737x2012000300004 ◽

2012 ◽

Vol 59 (3) ◽

pp. 313-320

Author(s):

Kelly Cristine de Sousa Pontes ◽

Andréa Pacheco Batista Borges ◽

Renato Barros Eleotério ◽

Emily Correna Carlo Reis ◽

Tatiana Schmitz Duarte

Keyword(s):

Amniotic Membrane ◽

Room Temperature ◽

Repair Process ◽

Treated Group ◽

Control Group ◽

Vessel Formation ◽

Corneal Ulcers ◽

In The Beginning ◽

Amniotic Membranes ◽

Ocular Discharge

The aim of this study was to evaluate the effects of canine amniotic membrane, previously preserved in glycerin, used as a patch on the repair of experimentally-made superficial corneal ulcers and to compare corneal epithelization between the treated and non-treated groups. Xenogeneic amniotic membranes were collected aseptically and preserved in 99% glycerin at room temperature. Each animal was anesthetized and submitted to superficial corneal keratectomy of the left eye. The treated group received a fragment of canine amniotic membrane as a patch, while the control group had no treatment. The treated group showed blepharospasm, ocular discharge and conjunctival congestion. The membrane accelerated corneal repair in the beginning of the process, however, it delayed its conclusion (p<0.05). Treated eyes showed greater vessel formation and decreased corneal transparency (p<0.05). The stroma of the control group was thicker than that of the treated group (p<0.05). We suggest that amniotic membrane used in this manner can be applied as a therapy for superficial corneal ulcers in the beginning phases of the repair process.

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In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express ◽

10.1186/s13568-021-01269-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Masarra M. Sakr ◽

Walid F. Elkhatib ◽

Khaled M. Aboshanab ◽

Eman M. Mantawy ◽

Mahmoud A. Yassien ◽

...

Keyword(s):

Survival Rate ◽

Murine Model ◽

Histopathological Examination ◽

Healing Process ◽

Bacterial Count ◽

Treated Group ◽

Control Group ◽

In Vivo Evaluation ◽

Systemic Spread

AbstractFailure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

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Effects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane

Cells Tissues Organs ◽

10.1159/000515448 ◽

2021 ◽

pp. 1-11

Author(s):

Jéssica Tereza Guedes de Oliveira Moraes ◽

Maíra Maftoum Costa ◽

Paula Cristina Santos Alves ◽

Luciana Barros Sant’Anna

Keyword(s):

Regenerative Medicine ◽

Amniotic Membrane ◽

Structural Integrity ◽

Storage Period ◽

Soluble Proteins ◽

Control Group ◽

Human Amniotic Membrane ◽

Glycerol Solution ◽

Preservation Methods ◽

Preservation Method

The human amniotic membrane (AM) is emerging as an interesting biomaterial for regenerative medicine due to its biological and mechanical proprieties. The beneficial effects of the AM are probably related to its bioactive factors produced by local cells and stored in the stromal matrix. However, the search for a preservation method capable of preserving AM properties remains a challenge. The aim of this study was to evaluate important features of 2 anatomical regions of the human AM (reflected and placental amnion) after different preservation methods. For this purpose, human placentas were harvested and processed for AM isolation and storage at 2 different conditions: room temperature for 18 h in DMEM (fresh AM) and −80°C in DMEM/glycerol solution for 30 days (cryopreserved AM). After the storage period, the structural integrity of the membrane was assessed by histological and Picrosirius polarization analysis, cellular viability analysis was performed using the MTT assay, and the soluble proteins were quantified with the Qubit Protein Assay Kit. Both preservation protocols reduced the cell viability, mainly in the placental amnion region of the AM, but preserved the morphology of epithelial and stromal layers, as well as the organization and distribution of collagen fibers. There was a reduction in soluble proteins only in fresh AM. Importantly, the cryopreserved AM group presented the same concentration as the control group. In conclusion, the cryopreservation using DMEM/glycerol was ideal for preserving the structural integrity and soluble protein content, indicating the feasibility of this method in preserving AM for its use in regenerative medicine.

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Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v31i4.2724 ◽

2019 ◽

Vol 31 (4) ◽

Author(s):

Nawar Bahjet Kamil ◽

Nada M. Al-Ghaban

Keyword(s):

Healing Process ◽

Inflammatory Cells ◽

Control Group ◽

Facial Skin ◽

Histological Findings ◽

Control Groups ◽

Mean Values ◽

Significant Difference ◽

The Right ◽

New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

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Effect of Pistacia atlantica subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers

Molecules ◽

10.3390/molecules25245819 ◽

2020 ◽

Vol 25 (24) ◽

pp. 5819

Author(s):

Shokhan H. Azeez ◽

Shanaz M. Gaphor ◽

Aram M. Sha ◽

Balkees T. Garib

Keyword(s):

Wistar Rats ◽

Bone Markers ◽

Inflammatory Cells ◽

Treated Group ◽

Surrounding Tissue ◽

Control Group ◽

Pistacia Atlantica ◽

Male Wistar Rats ◽

Experimental Periodontitis ◽

Serum Rankl

The aim of this study was to assess the effect of local application of essential oil of Pistacia atlantica kurdica (EOK) gel in treatment of experimentally induced periodontitis in rats and its effect on osteoclastogenic bone markers. Twenty-four male Wistar rats of 250 to 350 g were used in this study and were allocated into four groups. Control negative (without induced periodontitis), control positive (induced experimental periodontitis left without treatment), treatment control (induced experimental periodontitis and treated with Chlorhexidine gel) and EOK treated group (induced experimental periodontitis treated with EOK gel). The animals were sacrificed after 30 days, and the mandibular central incisor and surrounding tissue were dissected from the mandible and further processed for preparing H&E slides. Inflammatory cells, osteoclast cells, and periodontal ligament (PDL) were examined and measured histologically. Finally, the mean concentrations of both markers, receptor activator of nuclear factor kappa-Β ligand (RANKL) and (Interleukin-1β) IL-1β, were analyzed by ELISA. A significant reduction of inflammatory reaction and osteoclast numbers with improvement of PDL and low mean concentrations of RANKL and IL-1β were seen in the EOK treated group in comparison to the control group and the chlorhexidine group as well. The extract showed a protective effect in the healing of periodontitis that had been induced in rats and decreased bone resorption by down regulation of serum RANKL and IL-1β markers.

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Influence of nicotine on healing of vesical sutures in rabbits

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502007000600012 ◽

2007 ◽

Vol 22 (6) ◽

pp. 479-484 ◽

Cited By ~ 3

Author(s):

Christiano Machado ◽

Eduardo Wei Kin Chin ◽

Sérgio Ossamu Ioshii ◽

Renato Tâmbara Filho ◽

Aline Von Bathen

Keyword(s):

Tensile Strength ◽

Statistical Difference ◽

Saline Solution ◽

Healing Process ◽

Bladder Wall ◽

Inflammatory Cells ◽

Strength Test ◽

Control Group ◽

Time Intervals ◽

Tensile Strength Test

PURPOSE: To study the effects of nicotine on inflammatory cells, deposition of collagen and its interference on the strength of tissue in vesical sutures in rabbits. METHODS: Twenty New Zealand rabbits were used, randomized into two groups: group N, consisting of 10 animals, to which nicotine was administered in the dose of 2mg/kg of weight as a subcutaneous injection, diluted in 1ml of saline solution at 0.9% in a daily administration during the 28 days prior to the surgery; and group C, consisting of 10 animals, to which saline solution at 0.9% was administered in the same conditions and time intervals of the nicotine group. All the animals underwent cystotomy and suture of the bladder wall 28 days after the administration of nicotine or saline solution. The measurements were performed on the fourth and seventh day in each group after cystectomy and euthanasia of the animals. A fragment of bladder was removed and sent for a tensile strength test to evaluate the tissue strength and another fragment underwent a histological analysis of inflammatory process and deposition of collagen. RESULTS: There was a decrease of neutrophils on the fourth postoperative day (p=0.079) and an increase of plasmocytes on the seventh postoperative day (p=0.053) in the animals that were given nicotine, without statistical difference in relation to the control group. In the analysis of the proliferation of fibroblasts, deposition of collagen and tensile strength test, there was no statistical difference in relation to the control group. CONCLUSION: The administration of nicotine in rabbits did not influence the healing process of vesical suture in relation to the inflammatory cells, deposit of collagen and tissue strength of the suture.

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Biological effects of amniotic membrane on diabetic foot wounds: a systematic review

Journal of Wound Care ◽

10.12968/jowc.2018.27.sup2.s19 ◽

2018 ◽

Vol 27 (Sup2) ◽

pp. S19-S25 ◽

Cited By ~ 9

Author(s):

André Oliveira Paggiaro ◽

Andriws Garcia Menezes ◽

Alexandra Donizetti Ferrassi ◽

Viviane Fernandes De Carvalho ◽

Rolf Gemperli

Keyword(s):

Systematic Review ◽

Wound Healing ◽

Amniotic Membrane ◽

Diabetic Foot ◽

Biological Effects ◽

Meta Analysis ◽

Healing Process ◽

Intervention Group ◽

Risk Of Bias ◽

Control Group

Objective: The amniotic membrane has biological properties that are beneficial to the wound healing process of diabetic foot ulcers (DFU). Our aim is to analyse the scientific evidence found in literature on the use of the amniotic membrane to stimulate DFU healing. Method: A systematic review of amniotic membrane's influence was undertaken, using the search terms ‘placenta’ ‘diabetic foot’ ‘amnion’ and biological dressing’, assessing the outcomes ‘wound healing’ and ‘wound healing time’, in DFU. Following the inclusion and exclusion criteria, randomised controlled trials (RCT) were identified, and the risk of bias was analysed according to the Cochrane risk of bias tool. We conducted a meta-analysis of the two outcomes to evaluate the level of evidence. Results: We identified six clinical trials, with a total of 331 patients. The most common risks of bias in the studies were selection, attrition, and detection biases. From the meta-analysis, the difference of the intervention group (amnion) in relation to the control group was statistically significant. We found that wound healing in the group treated with amniotic membrane occurs 2.32 times more often and is 32 days faster in comparison with the group that used conventional dressings. Conclusion: There is statistical evidence to support the effectiveness of amniotic membrane in comparison with other conventional dressings. In addition, there is a clear tendency for the use of amniotic membrane treatment to result in a larger number of DFUs healing at a quicker rate.

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