Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

ABSTRACT In 2016, several reports emerged of fresh-market staked tomato plants with severe symptoms of pith necrosis and premature death in commercial cultivation areas in the states of Paraná and Minas Gerais, Brazil, which are similar to those caused by Pseudomonas corrugata. Four bacterial strains were isolated from infected tissue samples and characterized as Gram-negative, aerobic, and fluorescent on King’s B Medium. LOPAT tests were performed and the isolate UFU H120 was classified into group Vb (+ + − + −) and the isolates UFU H3, UFU H6, and UFU H21 into group Va (− + − + −). The pathogenicity of the isolates was confirmed by artificial inoculation on tomato plants of Santa Cruz type, cv. Kada Gigante, and Koch’s postulates were accomplished successfully. The isolates sequences of their 16S rRNA gene region were compared with those deposited in GenBank, the isolate UFU H120 aligning with Pseudomonas fluorescens (99,45% similarity) and the isolates UFU H3, UFU H6, and UFU H21 with Pseudomonas putida (99.2, 99.53 and 99.64% similarity, respectively). Notably, P. fluorescens and P. putida are most known as saprophytic bacteria normally present in the soil, although both of these species have already been reported to infect tomato plants in Italy. However, so far, there have not been any reports of such bacteria being phytopathogenic on tomato or any crop in Brazil. Thus, to our knowledge, this is the first report of pith necrosis of such occurrence in Brazil.

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First Report of Tomato (Lycopersicon esculentum) Pith Necrosis Caused by Pseudomonas fluorescens and P. corrugata in the Kingdom of Saudi Arabia

Plant Disease ◽

10.1094/pd-91-0110b ◽

2007 ◽

Vol 91 (1) ◽

pp. 110-110 ◽

Cited By ~ 5

Author(s):

Y. Molan ◽

Y. Ibrahim

Keyword(s):

Saudi Arabia ◽

Lycopersicon Esculentum ◽

Pseudomonas Fluorescens ◽

Nutrient Agar ◽

Bacterial Suspension ◽

Distilled Water ◽

Tomato Plants ◽

Arginine Dihydrolase ◽

Biolog Analysis ◽

Pith Necrosis

From 2002 to 2004, tomato (Lycopersicon esculentum) plants with external stem lesions, adventitious roots, and necrotic pith that was hollowed or chambered were received by the Clinical Lab of the Plant Protection Department from eight greenhouses in the Riyadh, Abha, and El-Kharj regions of Saudi Arabia. Bacteria were isolated on nutrient agar or King's medium B (KMB) from the stems of tomato plants cv. Red Gold, the cultivar most commonly grown in greenhouses. Gram-negative, rod-shaped bacteria were consistently isolated from stems with symptoms of pith necrosis. They were identified as Pseudomonas fluorescens (biotype I) and P. corrugata on the basis of morphological, physiological, and biochemical tests (2). Isolates of P. fluorescens isolated from Abha and El-Kharj were fluorescent on KMB, aerobic, and positive for oxidase, arginine dihydrolase, and gelatin liquefaction. Furthermore, all isolates produced levan-type colonies on sucrose nutrient agar and utilized glucose, 2-ketogluconate, sucrose, and sorbitol. They were negative for tobacco hypersensitivity and nitrate reduction. The strains of P. corrugata isolated from Riyadh were nonfluorescent, aerobic, and positive for oxidase, nitrate reductase, arginine dihydrolase, and utilization of malonate, alanine, trehalose, arginine, mannitol, and m-inositol. They were negative for levan, pectinase, tobacco hypersensitivity, and utilization of cellobiose and sorbitol. The identity of bacterial species was confirmed by Biolog analysis (carbon source utilization at 37°C), with a similarity index of 0.75 for P. corrugata and 0.71 for P. fluorescens. Four-week-old tomato plants (cv. Red Gold) were inoculated by injecting 50 μl of a bacterial suspension into the axils of the first true leaves. The bacterial suspension was prepared from 24-h-old cultures with sterile distilled water. Sterile distilled water was used as the negative control. After inoculation, plants were covered with polyethylene bags for 24 h to maintain high humidity at 25°C (1). Necrotic lesions surrounding injection points were observed 14 days after inoculation. At 4 weeks after inoculation, all inoculated plants showed symptoms of necrotic pith similar to those observed on the samples received. Control plants injected with water remained healthy throughout the experiments. Isolates of P. fluorescens (biotype I) and P. corrugata were reisolated from inoculated plants and were identical to the original strains on the basis of Biolog analysis. To our knowledge, this is the first report of tomato pith necrosis in Saudi Arabia. References: (1) G. Demir. J. Turk. Phytopathol. 19:63, 1990. (2) R. A. Lelliott and D. E. Stead. Methods for the Diagnosis of Bacterial Diseases of Plants. Blackwell Scientific Publications. Oxford, UK, 1987.

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Pseudomonas corrugata Causing Pith Necrosis on Tomato Plants in Baja California Sur, México

Plant Disease ◽

10.1094/pdis.2002.86.5.563b ◽

2002 ◽

Vol 86 (5) ◽

pp. 563-563

Author(s):

G. Rodríguez-Alvarado ◽

J. Holguín-Peña ◽

N. Ochoa-Álvarez ◽

S. P. Fernández-Pavía ◽

J. A. Geraldo-Verdugo

Keyword(s):

Baja California ◽

Tap Water ◽

Baja California Peninsula ◽

Bacterial Suspension ◽

Petroleum Jelly ◽

Baja California Sur ◽

Fresh Market ◽

Pseudomonas Corrugata ◽

Tomato Plants ◽

Pith Necrosis

Fresh market tomato (Lycopersicon esculentum Mill.) cultivars are grown in field and greenhouse areas in Baja California Sur from October to June for international markets. During March and April 2001, field-grown tomato plants showing external necrotic stem lesions and hollowed necrotic pith were observed in a 50-ha field 30 km south of La Paz. The average disease incidence in the field was 3%. Most infected plants presented necrotic lesions in the main stem 20 to 30 cm above the soil line. A few plants also presented necrotic lesions in lateral branches. Transversally cut sections in the necrotic stem area showed rotting of the vascular system with hollow cavities, typical symptom of pith necrosis. To isolate the pathogen, 5-cm-long transverse portions of diseased stems were excised, washed with soap and brushed, and rinsed with tap water. The stem portions were soaked in 10% bleach for 2 min, blotted dry on sterile paper, and 1-cm2 sections were cut to include the margins of the necrotic pith. These sections were plated on nutrient agar and incubated at 28 to 30°C. Gram-negative, rod-shaped bacteria were consistently isolated from stems with pith necrosis. They were identified as Pseudomonas corrugata using Biolog analysis (carbon source utilization at 37°C), with a similarity index of 1.0. To confirm pathogenicity, experiments were conducted twice in a screenhouse on a total of 24 2-month-old tomato cv. Rutgers plants (50 to 60 cm tall). Bacteria were injected with a syringe into the stems above the point of lateral branching at two different sites, using 0.25 to 0.5 ml of a bacterial suspension (105 CFU/ml). Injection points were sealed after inoculation with a small amount of petroleum jelly. Necrotic lesions surrounding the point of injection were observed 10 days after inoculation. Four weeks after inoculation, plants showed necrotic pith symptoms similar to those observed on field-grown plants, and P. corrugata was recovered from the margins of areas with necrotic pith. Control plants, which were injected with water, remained healthy throughout the experiments. P. corrugata has been reported to cause pith necrosis in tomato plants in California (3), Florida (2), and the northern part of the Baja California peninsula (1). This report confirms the presence of P. corrugata in the Baja California peninsula, and to our knowledge, this is the first report of P. corrugata causing pith necrosis in tomato plants in the southern state of Baja California Sur, Mexico. References: (1) N. B. Carroll et al. N.C. Agric. Res. Serv. Tech. Bull. No. 300, 1992. (2) J. B. Jones et al. Plant Dis. 67:425, 1983. (3) M. Lai et al. Plant Dis. 67:110, 1983.

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Occurrence of pith necrosis caused by Pseudomonas fluorescens on tomato plants in Turkey

Plant Pathology ◽

10.1111/j.1365-3059.2004.01092.x ◽

2004 ◽

Vol 53 (6) ◽

pp. 803-803 ◽

Cited By ~ 6

Author(s):

H. Saygili ◽

Y. Aysan ◽

F. Sahin ◽

N. Ustun ◽

M. Mirik

Keyword(s):

Pseudomonas Fluorescens ◽

Tomato Plants ◽

Pith Necrosis

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STUDIES ON SOME BACTERIAL DISEASES IN OREOCHROMIS NILOTICUS WITH SPECIAL REFERENCES TO TREATMENT

Mansoura Veterinary Medical Journal ◽

10.35943//mvmj.2018.19.1414 ◽

2018 ◽

pp. 39-47

Author(s):

Viola Zaki ◽

Ahmed EL-gamal ◽

Yasmin Reyad

Keyword(s):

Oreochromis Niloticus ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

Histopathological Examination ◽

Klebsiella Oxytoca ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Tissue Samples ◽

Hydropic Degeneration ◽

Total Prevalence

he present research carried out to study the common bacterial infections in Oreochromis niloticus (Nile tilapia) in Manzala area at Dakahlia governorate and possible antimicrobial agents used for treatment. A total number of 400 fish were randomly collected from Manzala private farms at Dakahlia governorate and subjected to the clinical, bacteriological and histopathological examination. The highest prevalence of bacterial isolates during the whole period of examination of naturally infected O.niloticus was recorded for A.hydrophila (22.66%), followed by V.alginolyticus (19.01%), V.parahemolyticus (13.80%), Streptococcus spp. (12.24%), A.caviae (11.72%), V.cholera (10.16%), A.salmonicida (7.55%), while the lowest prevalence was recorded for Klebsiella oxytoca (2.86%). The seasonal highest total prevalence of bacterial isolates from examined naturally infected O. niloticus was recorded in spring (30.21%), followed by autumn (28.39%), then summer (22.40%) and the lowest prevalence was recorded in winter (19.01%). Histopathological findings of the tissue samples which collected from different organs of naturally infected O.niloticus revealed that spleen show marked hemosiderosis and sever hemorrhage, gills showsever congestion of lamellar capillaries with marked aneurysm, necrosis and hemorrhage of lamellar epithelium and liver show sever hydropic degeneration and necrosis of hepatocytes, Ciprofloxacin was the most effective antibiotic against all isolated bacterial strains

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Campylobacter insulaenigrae bacteremia with meningitis: a case report

BMC Infectious Diseases ◽

10.1186/s12879-021-06353-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Moe Kyotani ◽

Tsuneaki Kenzaka ◽

Hozuka Akita ◽

Soichi Arakawa

Keyword(s):

Blood Culture ◽

Marine Mammals ◽

Chronic Subdural Hematoma ◽

16S Rrna Gene Sequencing ◽

Enhancement Effect ◽

Rrna Gene ◽

Clinical Report ◽

Bacterial Strains ◽

Fisher Syndrome ◽

First Case

Abstract Background The bacterium Campylobacter insulaenigrae was first isolated from marine mammals of Scotland in 2004. Only one case of C. insulaenigrae infection in humans has been previously reported. Case presentation An 89-year-old Japanese man without dementia was admitted to our hospital, because he presented with a fever of 38 °C and weakness in right leg since 5 days. He had organized chronic subdural hematoma (CSH), and no history of pre-infection. At the time of admission, he had paralysis of the extraocular muscle, ataxia, and low manual muscle test score of the right side. He was suspected to have Miller Fisher syndrome; however, these symptoms improved without any treatment. On day 22 in the hospital, the patient presented a fever of 38.8 °C, left cranial nerve disorder, and hemiplegia. On day 25, the patient presented with signs of meningeal irritation; cerebrospinal fluid examination indicated an increase in the number of apocytes and a low glucose level. A contrast magnetic resonance imaging (MRI) scan of the patient’s head indicated a contrast enhancement effect in his right meninges. The blood culture showed presence of spirillums; 16S rRNA gene sequencing confirmed that the spirillums in the blood culture were Campylobacter insulaenigrae (C. insulaenigrae). We started treatment with meropenem for bacteremia and meningitis. When the symptoms improved, meropenem was replaced with ampicillin, based on the result of the drug sensitivity test. The treatment continued for 4 weeks. Conclusions We report the first case of meningitis caused by C. insulaenigrae bacteremia in humans, and the second clinical report of C. insulaenigrae infection in humans. The bacterial strains isolated from humans and marine mammals had different genotypes. This suggests that different genotypes could be responsible for differences in the hosts. Further case studies are needed to establish the reasons behind the difference in the manifestations of C. insulaenigrae infections reported so far.

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Microbiome of Odontogenic Abscesses

Microorganisms ◽

10.3390/microorganisms9061307 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1307

Author(s):

Sebastian Böttger ◽

Silke Zechel-Gran ◽

Daniel Schmermund ◽

Philipp Streckbein ◽

Jan-Falco Wilbrand ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Oral Microbiome ◽

Minor Role ◽

Rrna Gene ◽

Sequencing Analysis ◽

Bacterial Strains ◽

16S Rrna Gene Analysis ◽

Odontogenic Infections ◽

Odontogenic Abscess

Severe odontogenic abscesses are regularly caused by bacteria of the physiological oral microbiome. However, the culture of these bacteria is often prone to errors and sometimes does not result in any bacterial growth. Furthermore, various authors found completely different bacterial spectra in odontogenic abscesses. Experimental 16S rRNA gene next-generation sequencing analysis was used to identify the microbiome of the saliva and the pus in patients with a severe odontogenic infection. The microbiome of the saliva and the pus was determined for 50 patients with a severe odontogenic abscess. Perimandibular and submandibular abscesses were the most commonly observed diseases at 15 (30%) patients each. Polymicrobial infections were observed in 48 (96%) cases, while the picture of a mono-infection only occurred twice (4%). On average, 31.44 (±12.09) bacterial genera were detected in the pus and 41.32 (±9.00) in the saliva. In most cases, a predominantly anaerobic bacterial spectrum was found in the pus, while saliva showed a similar oral microbiome to healthy individuals. In the majority of cases, odontogenic infections are polymicrobial. Our results indicate that these are mainly caused by anaerobic bacterial strains and that aerobic and facultative anaerobe bacteria seem to play a more minor role than previously described by other authors. The 16S rRNA gene analysis detects significantly more bacteria than conventional methods and molecular methods should therefore become a part of routine diagnostics in medical microbiology.

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Identification and Genomic Characterization of Pathogenic Bacillus altitudinis from Common Pear Trees in Morocco

Agronomy ◽

10.3390/agronomy11071344 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1344

Author(s):

Naima Lemjiber ◽

Khalid Naamani ◽

Annabelle Merieau ◽

Abdelhi Dihazi ◽

Nawal Zhar ◽

...

Keyword(s):

16S Rrna Gene Sequencing ◽

Genomic Islands ◽

Rrna Gene ◽

In Planta ◽

Bacterial Strains ◽

Bacillus Altitudinis ◽

Genes Encoding ◽

Bacillus Spp ◽

Pear Trees ◽

Rrna Gene Sequencing

Bacterial burn is one of the major diseases affecting pear trees worldwide, with serious impacts on producers and economy. In Morocco, several pear trees (Pyrus communis) have shown leaf burns since 2015. To characterize the causal agent of this disease, we isolated fourteen bacterial strains from different parts of symptomatic pear trees (leaves, shoots, fruits and flowers) that were tested in planta for their pathogenicity on Louise bonne and Williams cultivars. The results showed necrotic lesions with a significant severity range from 47.63 to 57.77% on leaves of the Louise bonne cultivar inoculated with isolate B10, while the other bacterial isolates did not induce any disease symptom. 16S rRNA gene sequencing did not allow robust taxonomic discrimination of the incriminated isolate. Thus, we conducted whole-genome sequencing (WGS) and phylogenetic analyzes based on gyrA, gyrB and cdaA gene sequences, indicating that this isolate belongs to the Bacillus altitudinis species. This taxonomic classification was further confirmed by the Average Nucleotide Identity (ANI) and the in silico DNA-DNA hybridization (isDDH) analyzes compared to sixty-five Bacillus spp. type strains. The genome was mined for genes encoding carbohydrate-active enzymes (CAZymes) known to play a role in the vegetal tissue degradation. 177 candidates with functions that may support the in planta phytopathogenicity results were identified. To the best of our knowledge, this is the first data reporting B. altitudinis as agent of leaf burn in P. communis in Morocco. Our dataset will improve our knowledge on spread and pathogenicity of B. altitudinis genotypes that appears as emergent phytopathogenic agent, unveiling virulence factors and their genomic location (i.e., within genomic islands or the accessory genome) to induce trees disease.

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Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.010728-0 ◽

2010 ◽

Vol 60 (3) ◽

pp. 603-609 ◽

Cited By ~ 12

Author(s):

Lyudmila A. Romanenko ◽

Naoto Tanaka ◽

Galina M. Frolova

Keyword(s):

Sea Of Japan ◽

Sequence Similarity ◽

Fatty Acid Analysis ◽

Phenotypic Characterization ◽

The Sea Of Japan ◽

Rrna Gene ◽

Phylogenetic Distance ◽

The Novel ◽

Bacterial Strains ◽

Separate Branch

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

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Rhizobium oryzicola sp. nov., potential plant-growth-promoting endophytic bacteria isolated from rice roots

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.000358 ◽

2015 ◽

Vol 65 (Pt_9) ◽

pp. 2931-2936 ◽

Cited By ~ 21

Author(s):

Xiao-Xia Zhang ◽

Ju-Sheng Gao ◽

Yan-Hua Cao ◽

Rizwan Ali Sheirdil ◽

Xiu-Cheng Wang ◽

...

Keyword(s):

Type Strain ◽

Housekeeping Genes ◽

Rrna Gene ◽

Bacterial Strains ◽

Astragalus Sinicus ◽

Dna Relatedness ◽

Rice Roots ◽

Long Term Experiment ◽

Plant Growth Promoting

Bacterial strains ZYY136T and ZYY9 were isolated from surface-sterilized rice roots from a long-term experiment of rice–rice–Astragalus sinicus rotation. The 16S rRNA gene sequences of strains ZYY136T and ZYY9 showed the highest similarity, of 97.0 %, to Rhizobium tarimense PL-41T. Sequence analysis of the housekeeping genes recA, thrC and atpD clearly differentiated the isolates from currently described species of the genus Rhizobium. The DNA–DNA relatedness value between ZYY136T and ZYY9 was 82.3 %, and ZYY136T showed 34.0 % DNA–DNA relatedness with the most closely related type strain, R. tarimense PL-41T. The DNA G+C content of strain ZYY136T was 58.1 mol%. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C16 : 0 3-OH. Strains ZYY136T and ZYY9 could be differentiated from the previously defined species of the genus Rhizobium by several phenotypic characteristics. Therefore, we conclude that strains ZYY136T and ZYY9 represent a novel species of the genus Rhizobium, for which the name Rhizobium oryzicola sp. nov. is proposed (type strain ZYY136T = ACCC 05753T = KCTC 32088T).

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Chitinophaga oryzae sp. nov., an epiphytic bacterium isolated from rice root surfaces

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.004926 ◽

2021 ◽

Vol 71 (7) ◽

Author(s):

Nantawan Niemhom ◽

Chanwit Suriyachadkun ◽

Chokchai Kittiwongwattana

Keyword(s):

Type Species ◽

Rrna Gene ◽

Major Polar Lipid ◽

Bacterial Strains ◽

Respiratory Quinone ◽

Content Type ◽

Link Type ◽

Major Respiratory Quinone ◽

Phylogenomic Analyses ◽

Close Relatives

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains were isolated from the surfaces of rice roots. They were designated as strains 1303T and 1310. Their colonies were circular, entire, opaque, convex and yellow. They were chitinase- and catalase-positive, reduced nitrate and grew at 16–37 °C (optimum, 30 °C), pH 5.0–10.0 (optimum, pH 7.0) and 0–2.0% NaCl (optimum, 1.0 %). Based on the 16S rRNA gene sequence analysis, they were classified as members of the genus Chitinophaga . Results of phylogenetic and phylogenomic analyses indicated that they formed a cluster with Chitinophaga eiseniae YC6729T, Chitinophaga qingshengii JN246T, Chitinophaga varians 10-7 W-9003T and Chitinophaga fulva G-6-1-13T. When the genomic sequences of strains 1303T and 1310 were compared with their close relatives, the average nucleotide identity and digital DNA–DNA hybridization values were below the cut-off levels. Phosphatidylethanolamine was the major polar lipid. MK-7 was the major respiratory quinone. iso-C15 : 0, C16 : 1 ω5c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c) were the predominant fatty acids. Differential characteristics between both strains and their close relatives were also observed. Based on the distinctions in genotypic, phenotypic and chemotypic features, strains 1303T and 1310 represent members of a novel species of the genus Chitinophaga , for which the name Chitinophaga oryzae sp. nov. is proposed. The type strain is 1303T (=KACC 22075T=TBRC 12926T).

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