scholarly journals Carrot seed germination and ethylene production at high temperature in response to seed osmopriming

2013 ◽  
Vol 31 (4) ◽  
pp. 554-558 ◽  
Author(s):  
Warley M Nascimento ◽  
Donald J Huber ◽  
Daniel J Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Daucus Carota ◽  
Ethylene Production ◽  
Primary Root ◽  
Seed Priming ◽  
Germination Percentage ◽  
Light Conditions ◽  
Carrot Seed

Carrot (Daucus carota) seed germination can be erratic or reduced under temperatures above 35ºC. Priming circumvents seed thermoinhibition in several crops, including carrot. The involvement of ethylene in germination at supra-optimal temperatures has been reported in other species, but its role in carrot seed germination has not been examined. The objective of this study was to determine whether priming-mediated improvement in carrot seed germination at high temperature was associated with increased ethylene production. Seeds of two tropical carrot genotypes (Alvorada and Brasília) were incubated over a range of temperatures. 'Brasilia' seeds were primed for 72 hours in aerated PEG solution at 15ºC under light conditions and germination tested at 20 and 35ºC. Seeds of a thermosensitive genotype 'Arrowhead' were primed for 0, 4, 8 and 12 days in aerated PEG 6000 solutions at 15ºC under light conditions. Seeds were incubated at 20 and 35ºC, and ethylene production was measured immediately before visible primary root protrusion. Temperatures above 30ºC decreased seed germination percentage of 'Alvorada' and 'Brasilia'. Priming increased 'Brasilia' seed germination at 35ºC. 'Arrowhead' germinated 96% at 20ºC, and 13% at 35ºC. Seed germination and ethylene production of 'Arrowhead', a thermosensitive genotype, increased in response to increased duration of priming. The results suggest that seed priming circumvents thermoinhibition of carrot seed germination by increasing ethylene production at high temperatures.

scholarly journals Carrot Seed Germination at High Temperature: Effect of Genotype and Association with Ethylene Production

HortScience ◽  
2008 ◽  
Vol 43 (5) ◽  
pp. 1538-1543 ◽  
Author(s):  
Warley M. Nascimento ◽  
Jairo V. Vieira ◽  
Giovani O. Silva ◽  
Kathleen R. Reitsma ◽  
Daniel J. Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Ethylene Production ◽  
Plant Introduction ◽  
Negative Effects ◽  
The North ◽  
Commercial Cultivars ◽  
North Central Regional ◽  
Carrot Seed

Carrot (Daucus carota L.) seed germination may be erratic or reduced under high temperatures (above 35 °C). Even in tropical genotypes (tolerant to high temperatures during crop development), the negative effects of high temperatures on carrot stand establishment have been observed, especially during summer. The objectives of this study were to characterize commercial carrot cultivars and accession lines for their ability to germinate at high temperature and determine the ethylene production during imbibition at high temperature. Seeds from 34 commercial cultivars and 125 carrot accessions from the North Central Regional Plant Introduction Station were germinated at 25 °C (optimal) and 35 ± 0.5 °C (high) in constant light. Ethylene production during seed imbibition at high temperature was evaluated in some genotypes. Many of the commercial cultivars had reduced germination at 35 °C. ‘XPC-3617’, ‘Alvorada’, ‘Brasilia’, and ‘Esplanada’ had the greatest germination at 35 °C. A greater number of accessions germinated at 35 °C than did the commercial genotypes. The accession PI 319858 germinated 95% at both temperatures and was considered thermotolerant. Six accessions (Ames 7665, Ames 7698, Ames 25031, PI 167082, PI 294637, and PI 319858) germinated above 80% at 35 °C and were also identified as potential sources of thermotolerance. Fifteen other accessions (Ames 7694, Ames 25031, Ames 25036, Ames 25049, Ames 25705, PI 167082, PI 179687, PI 180834, PI 261782, PI 269486, PI 273658, PI 277710, PI 288242, PI 294637, and PI 319858) had thermotolerance ratios of T35/T25 0.85 or greater (where T35 = germination at 35 °C and T25 = germination at 25 °C) and were identified for further testing. The identified thermotolerant genotypes might be useful for carrot seed germination mechanism studies as well as for breeding programs. Ethylene production during seed germination at high temperature was greater in thermotolerant genotypes than in thermosensitive genotypes. High correlations were observed between first germination count at 35 °C and ethylene production, total germination at 35 °C and ethylene production, and thermotolerance ratio and ethylene production.

scholarly journals Priming Leek Seed for Improved Germination and Emergence at High Temperature

HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1077-1079 ◽  
Author(s):  
Carlos A. Parera ◽  
Daniel J. Cantliffe
Keyword(s):  
Polyethylene Glycol ◽  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Germination Percentage ◽  
Controlled Environment ◽  
Greenhouse Study ◽  
Growth Chambers ◽  
Coefficient Of Velocity ◽  
Peg 8000

`Verina' leek (AIlium porrum L.) seed germination is normally reduced at temperatures > 25C. Leek seeds were primed in aerated solutions (1.5 MPa, 10 days at 15C) of d-mannitol (mannitol), polyethylene glycol-8000 (PEG), KNO, and a nonaerated solution of PEG-8000 (PEG). At high temperatures mannitol, PEG, and PEG significantly enhanced germination percentage relative to KNO, or the control. At constant 30C, the mannitol, PEG, and PEG treatments increased final germination almost 10 times and the coefficient of velocity (COV) was improved compared to KNO, and the control. 10 growth chambers with alternating day/night temperatures (38 to 28C or 32 to 22C, 10 to 14 hours, respectively), primed seeds had significantly higher emergence and a larger COV than the control. In a greenhouse study under good conditions for germination, total emergence of primed and nonprimed seeds was similar; however, mannitol, PEG, and PEG led to a significantly higher COV than the control or KNO, treatments. These controlled-environment results demonstrate that priming leek seeds via mannitol, PEG, and PEG may promote early emergence at high temperature and improve stand uniformity for container transplant production.

scholarly journals Effect of Temperature, Light, and Storage Time on the Seed Germination of Pinus bungeana Zucc. ex Endl.: The Role of Seed-Covering Layers and Abscisic Acid Changes

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 300 ◽  
Author(s):  
Congcong Guo ◽  
Yongbao Shen ◽  
Fenghou Shi
Keyword(s):  
Abscisic Acid ◽  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Germination Percentage ◽  
Effect Of Temperature ◽  
Inhibition Mechanism ◽  
Radicle Protrusion ◽  
Pinus Bungeana ◽  
And Storage

Pinus bungeana Zucc. ex Endl. is an endemic conifer tree species in China with high ornamental value. In order to investigate favorable conditions for seed germination and explore the germination inhibition mechanism of this species at high temperatures, the effects of temperature, light, and storage on the mean germination time (MGT), speed of germination (SG), and total germination percentage (TGP) are evaluated here. Seeds that have either been kept still or entered into a state of dormancy at high temperature are assessed here by a recovery experiment. Furthermore, the contribution of covering layers on thermo-inhibition is analyzed here, including the way they work. This has been realized by the structural observation and via the determination of the abscisic acid (ABA) content. The results show that seeds germinate to a high percentage (approximately 90%) at temperatures of 15 or 20 °C, with or without light, whereas higher temperatures of 25 or 30 °C impeded radicle protrusion and resulted in the germination percentage decreasing sharply (within 5%). Inhibition at high temperatures was thoroughly reversed (bringing about approximately 80% germination) by placing the ungerminated seeds in favorable temperatures and incubating them for an additional 30 days. Dry cold storage did little to reduce the temperature request for germination. Embryo coverings, especially the nucellar membrane, and ABA levels both had a dominant role in seed germination regulation in response to temperature. Under favorable temperature conditions, the levels of ABA significantly decreased. Germination occurred when the levels dropped to a threshold of 15 ng/g (FW (Fresh Weight)). Incubation at a high temperature (25 °C) greatly increased ABA levels and caused the inhibition of radicle protrusion.

scholarly journals Evaluation of Lettuce Genotypes for Seed Thermotolerance

HortScience ◽  
2013 ◽  
Vol 48 (6) ◽  
pp. 708-714 ◽  
Author(s):  
Abbas Lafta ◽  
Beiquan Mou
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Seedling Emergence ◽  
Seed Priming ◽  
High Temperature Stress ◽  
Germination Percentage ◽  
Production Costs ◽  
Land Cost ◽  
Red Giant ◽  
Germplasm Accessions

Thermoinhibition of lettuce (Lactuca sativa L.) seed germination is a common problem associated with lettuce production. Depending on lettuce cultivars, seed germination may be inhibited when temperatures exceed 28 °C. The delay or inhibition of seed germination at high temperatures may reduce seedling emergence and stand establishment of lettuce in the field, leading to a reduction in economic yield. To identify heat-tolerant lettuce genotypes, lettuce varieties and germplasm accessions were screened for the ability to germinate under high-temperature stress. Twenty-four to 26 genotypes were selected from each lettuce types (crisphead, romaine, butterhead, loose leaf, and wild species) and their seeds were placed in petri dishes to test their ability to germinate at high temperatures (29 and 34 °C) as compared with controls at 24 °C. Some lettuce genotypes showed thermotolerance to 34 °C (less than 20% reduction in germination) such as Elizabeth, PI 342533, PI 358025, Florida Buttercrisp, Kordaat, Corsair, FL 50105, PRO 425, PI 278070, Noemie, Picarde, Gaillarde, L. serriola (PI 491112, UC96US23, PI 491147), L. virosa (PI 274378 D), L. saligna (PI 491159), and primitive (PI 187238 A, PI 289063 C). The germination rates were consistent with the germination percentage at the high temperatures. Seed germination in the field was very low and positively correlated with seed germination at 29 and 34 °C. The highest field germination percentages (greater than 40%) were observed in Belluro, Mantilia, Mid Queen, Headmaster, PRO 874, PRO 425, FL 50105, Corsair, Romaine SSC 1148, Romaine Romea, Green Forest, Grenadier, FL 43007, Squadron, Xena, Noemie, Green Wave, Picarde, and Red Giant. The results of this study indicated that lettuce genotypes differ greatly in their ability to germinate at high temperatures as determined by the percentages and the rates of germination. Our research indicates that thermoinsensitive varieties could be used to expand lettuce production seasons in warm and low land cost areas and reduce the need for seed priming, lowering the production costs. The information may also be useful for growers to better choose cultivars for warm environments and for lettuce breeders to improve the crop for adaptation to global warming and climate change.

scholarly journals Enhanced Celery Germination at Stress Temperature via Solid Matrix Priming

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 20-22 ◽  
Author(s):  
Carlos A. Parera ◽  
Ping Qiao ◽  
Daniel J. Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Germination Percentage ◽  
Apium Graveolens ◽  
Solid Matrix ◽  
Calcined Clay ◽  
Celery Seed ◽  
Negative Effect ◽  
Celery Seeds

To alleviate high-temperature-induced reductions in seed germination, emergence, and seedling uniformity in celery (Apium graveolens L.), seeds were primed via solid matrix priming (SMP); 0.5 g celery seeds was incubated at 15C with 10 g calcined clay and 2 ml water. After 2 days, 1, 2, 3, or 4 ml water or 1% NaOCl solution was added and seeds were kept for 2, 4, 6, 8, 10, 12, and 14 additional days. The germination percentage and coefficient of germination velocity (COV) were calculated for each treatment at 15 and 30C. The seeds primed with NaOCl gained significantly less moisture after 8 days of priming than those treated with water, regardless of the volume added. Germination of nonprimed seeds was 83% and 2% at 15 and 30C, respectively. Final germination at 30C increased to >80% when seeds were primed with 3 or 4 ml NaOCl solution or 3 ml water for >10 days. SMP treatment significantly reduced the negative effect of high temperature on celery seed germination.

scholarly journals Two-Hour Magneto-Priming with Static Magnetic Fields Ranging from 65 ± 3 to 505 ± 8 mT Does Not Improve the Germination Percentage of Industrial Hemp Seed at a Sub-Optimal Germination Temperature

J ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 192-196
Author(s):  
Kathrin Spendier
Keyword(s):  
Seed Germination ◽  
Magnetic Fields ◽  
Cannabis Sativa ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hemp Seed ◽  
Static Magnetic Fields ◽  
Beneficial Effects ◽  
Industrial Hemp ◽  
The U.S

Industrial hemp is a non-psychoactive variety of Cannabis sativa L., i.e., it contains less than 0.3% tetrahydrocannabinols (THC). This crop is one of historical importance in the U.S. as manufacturers seek industrial hemp as a renewable and sustainable resource for a wide variety of consumer and industrial products. To help farmers succeed, agronomic research on industrial hemp is needed. In this trial, investigations were performed to determine whether magneto-priming, a form of seed priming that involves magnetic fields, effects the germination percentage of industrial hemp seed. Beneficial effects of magnetic fields on seedling growths and germination have been reported for many different plant species. Dry industrial hemp seed was exposed to static magnetic fields ranging from 65 ± 3 to 505 ± 8 mT for 2 h prior to seed germination. Germination was performed at 13.6 ± 0.7 °C, a temperature that is representative of the germination temperatures of industrial hemp in the U.S. state of Colorado. Magneto-priming of seed had no statistically significant effect on seed germination percentage.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Influence of seed priming on germination characteristics of sorghum (Sorghum bicolor L. Moench)

2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Primary Root ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Seed Vigor ◽  
Germination Characteristics ◽  
Positive Effect

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.

scholarly journals Effect of Calcium Nitrate and Potassium Nitrate Priming on Seed Germination and Seedling Vigour of Papaya (Carica papaya L.)

2019 ◽  
Vol 14 (2) ◽  
pp. 149-154
Author(s):  
Maneesha S R ◽  
Priya Devi S
Keyword(s):  
Seed Germination ◽  
Calcium Nitrate ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Shoot Length ◽  
Potassium Ions ◽  
Seedling Vigour ◽  
Fresh Biomass ◽  
Seedling Characteristics

The effect of seed priming with calcium nitrate Ca (NO3)2 and potassium nitrate (KNO3) on germination and seedling vigour were studied in papaya varieties. Open pollinated local (gauty) papaya seeds were soaked in Ca (NO3)2 and KNO3 solutions (10000 ppm, 15000 ppm and 20000 ppm) up to 24 hours and germination percentage and seedling characteristics were recorded. The least number of days taken for seed germination (4.33 days), the highest germination percentage (82.56 % ), the highest shoot length (14.31 cm) the highest fresh biomass (1.36 g) and dry biomass (0.174 g) were recorded in 10000 ppm   Ca(NO3)2 treatment. Further, seeds of papaya varieties viz., Arka Surya, Arka Prabhat and Madhu bindhu were treated with 5000 ppm, 10000 ppm and 15000 ppm Ca (NO3)2 and observed that Arka Prabhat seeds treated with 10000 ppm Ca (NO3)2 hadtaken the least number of days for germination (4.75 days) and also the highest shoot length (25.2 cm). The results of the experiment proved the significant effect of calcium ions over potassium ions on papaya seed germination and seedling vigour.

Sign in / Sign up

Export Citation Format

Share Document