scholarly journals Priming Leek Seed for Improved Germination and Emergence at High Temperature

HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1077-1079 ◽  
Author(s):  
Carlos A. Parera ◽  
Daniel J. Cantliffe
Keyword(s):  
Polyethylene Glycol ◽  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Germination Percentage ◽  
Controlled Environment ◽  
Greenhouse Study ◽  
Growth Chambers ◽  
Coefficient Of Velocity ◽  
Peg 8000

`Verina' leek (AIlium porrum L.) seed germination is normally reduced at temperatures > 25C. Leek seeds were primed in aerated solutions (1.5 MPa, 10 days at 15C) of d-mannitol (mannitol), polyethylene glycol-8000 (PEG), KNO, and a nonaerated solution of PEG-8000 (PEG). At high temperatures mannitol, PEG, and PEG significantly enhanced germination percentage relative to KNO, or the control. At constant 30C, the mannitol, PEG, and PEG treatments increased final germination almost 10 times and the coefficient of velocity (COV) was improved compared to KNO, and the control. 10 growth chambers with alternating day/night temperatures (38 to 28C or 32 to 22C, 10 to 14 hours, respectively), primed seeds had significantly higher emergence and a larger COV than the control. In a greenhouse study under good conditions for germination, total emergence of primed and nonprimed seeds was similar; however, mannitol, PEG, and PEG led to a significantly higher COV than the control or KNO, treatments. These controlled-environment results demonstrate that priming leek seeds via mannitol, PEG, and PEG may promote early emergence at high temperature and improve stand uniformity for container transplant production.

scholarly journals Priming Improves High-temperature Germination of Pansy Seed

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 541-544 ◽  
Author(s):  
William J. Carpenter ◽  
Joseph F. Boucher
Keyword(s):  
Polyethylene Glycol ◽  
High Temperature ◽  
Plant Growth ◽  
Laboratory Studies ◽  
Optimum Conditions ◽  
Growing Medium ◽  
Growth Chambers ◽  
Seed Handling ◽  
Peg 8000

The optimum conditions for priming pansy (Viola × wittrockiana) seeds were in aerated osmotic solutions of polyethylene glycol 8000 (PEG 8000) at – 1.0 MPa for 7 days at 15C. Priming at – 0.8 MPa and 15C caused 8% to 26% of seeds to germinate during 4 to 13 days of priming. Increasing numbers of abnormal seedlings developed when priming was extended beyond 7 days. Final germination percentages were better in laboratory studies at 35C for primed (51%) than nonprimed (10%) seeds. Removal of the mucilage released by the seed with 240 g KOH or 170 g NaOH/liter for 15 or 30 seconds during priming did not affect total germination percentages, but did improve seed handling. Primed seed had higher, faster, and more uniform germination than nonprimed seeds after sowing in growing medium in plant growth chambers or greenhouses.

scholarly journals Effect of Temperature, Light, and Storage Time on the Seed Germination of Pinus bungeana Zucc. ex Endl.: The Role of Seed-Covering Layers and Abscisic Acid Changes

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 300 ◽  
Author(s):  
Congcong Guo ◽  
Yongbao Shen ◽  
Fenghou Shi
Keyword(s):  
Abscisic Acid ◽  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Germination Percentage ◽  
Effect Of Temperature ◽  
Inhibition Mechanism ◽  
Radicle Protrusion ◽  
Pinus Bungeana ◽  
And Storage

Pinus bungeana Zucc. ex Endl. is an endemic conifer tree species in China with high ornamental value. In order to investigate favorable conditions for seed germination and explore the germination inhibition mechanism of this species at high temperatures, the effects of temperature, light, and storage on the mean germination time (MGT), speed of germination (SG), and total germination percentage (TGP) are evaluated here. Seeds that have either been kept still or entered into a state of dormancy at high temperature are assessed here by a recovery experiment. Furthermore, the contribution of covering layers on thermo-inhibition is analyzed here, including the way they work. This has been realized by the structural observation and via the determination of the abscisic acid (ABA) content. The results show that seeds germinate to a high percentage (approximately 90%) at temperatures of 15 or 20 °C, with or without light, whereas higher temperatures of 25 or 30 °C impeded radicle protrusion and resulted in the germination percentage decreasing sharply (within 5%). Inhibition at high temperatures was thoroughly reversed (bringing about approximately 80% germination) by placing the ungerminated seeds in favorable temperatures and incubating them for an additional 30 days. Dry cold storage did little to reduce the temperature request for germination. Embryo coverings, especially the nucellar membrane, and ABA levels both had a dominant role in seed germination regulation in response to temperature. Under favorable temperature conditions, the levels of ABA significantly decreased. Germination occurred when the levels dropped to a threshold of 15 ng/g (FW (Fresh Weight)). Incubation at a high temperature (25 °C) greatly increased ABA levels and caused the inhibition of radicle protrusion.

scholarly journals Carrot seed germination and ethylene production at high temperature in response to seed osmopriming

2013 ◽  
Vol 31 (4) ◽  
pp. 554-558 ◽  
Author(s):  
Warley M Nascimento ◽  
Donald J Huber ◽  
Daniel J Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
High Temperatures ◽  
Daucus Carota ◽  
Ethylene Production ◽  
Primary Root ◽  
Seed Priming ◽  
Germination Percentage ◽  
Light Conditions ◽  
Carrot Seed

Carrot (Daucus carota) seed germination can be erratic or reduced under temperatures above 35ºC. Priming circumvents seed thermoinhibition in several crops, including carrot. The involvement of ethylene in germination at supra-optimal temperatures has been reported in other species, but its role in carrot seed germination has not been examined. The objective of this study was to determine whether priming-mediated improvement in carrot seed germination at high temperature was associated with increased ethylene production. Seeds of two tropical carrot genotypes (Alvorada and Brasília) were incubated over a range of temperatures. 'Brasilia' seeds were primed for 72 hours in aerated PEG solution at 15ºC under light conditions and germination tested at 20 and 35ºC. Seeds of a thermosensitive genotype 'Arrowhead' were primed for 0, 4, 8 and 12 days in aerated PEG 6000 solutions at 15ºC under light conditions. Seeds were incubated at 20 and 35ºC, and ethylene production was measured immediately before visible primary root protrusion. Temperatures above 30ºC decreased seed germination percentage of 'Alvorada' and 'Brasilia'. Priming increased 'Brasilia' seed germination at 35ºC. 'Arrowhead' germinated 96% at 20ºC, and 13% at 35ºC. Seed germination and ethylene production of 'Arrowhead', a thermosensitive genotype, increased in response to increased duration of priming. The results suggest that seed priming circumvents thermoinhibition of carrot seed germination by increasing ethylene production at high temperatures.

scholarly journals Effects of Matric and Osmotic Priming Treatments on Broccoli Seed Germination

1996 ◽  
Vol 121 (3) ◽  
pp. 423-429 ◽  
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum ◽  
Ronald D. Morse
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Root Growth ◽  
Growth Rates ◽  
Germination Rate ◽  
Membrane Integrity ◽  
Seed Vigor ◽  
Radicle Emergence ◽  
The Mean ◽  
Peg 8000

Priming, a controlled-hydration treatment followed by redrying, improves the germination and emergence of seeds from many species. We compared osmotic and matric priming to determine which was the most effective treatment for improving broccoli seed germination and to gain a greater understanding of how seed vigor is enhanced by priming. Broccoli (Brassica oleracea L. var. italica) seeds were osmotically primed in polyethylene glycol (PEG 8000) at -1.1 MPa or matrically primed in a ratio of 1.0 g seed:0.8 g synthetic calcium silicate (Micro-Cel E):1.8 ml water at -1.2 MPa. In the laboratory, germination rates and root lengths were recorded from 5 to 42C and 10 to 35C, respectively. Broccoli seeds germinated poorly at >35C. Root growth after germination was more sensitive to temperatures >30C and <15C than radicle emergence. Matric and osmotic priming increased germination rate in the laboratory, greenhouse, and field. However, matric priming had a greater effect on germination and root growth rates from 15 to 30C. Neither priming treatment affected minimum or maximum germination or root growth temperatures. Both priming treatments decreased the mean thermal time for germination by >35%. The greater germination performance of matrically primed seeds was most likely the result of increased oxygen availability during priming, increased seed Ca content, or improved membrane integrity.

scholarly journals Enhanced Celery Germination at Stress Temperature via Solid Matrix Priming

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 20-22 ◽  
Author(s):  
Carlos A. Parera ◽  
Ping Qiao ◽  
Daniel J. Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Germination Percentage ◽  
Apium Graveolens ◽  
Solid Matrix ◽  
Calcined Clay ◽  
Celery Seed ◽  
Negative Effect ◽  
Celery Seeds

To alleviate high-temperature-induced reductions in seed germination, emergence, and seedling uniformity in celery (Apium graveolens L.), seeds were primed via solid matrix priming (SMP); 0.5 g celery seeds was incubated at 15C with 10 g calcined clay and 2 ml water. After 2 days, 1, 2, 3, or 4 ml water or 1% NaOCl solution was added and seeds were kept for 2, 4, 6, 8, 10, 12, and 14 additional days. The germination percentage and coefficient of germination velocity (COV) were calculated for each treatment at 15 and 30C. The seeds primed with NaOCl gained significantly less moisture after 8 days of priming than those treated with water, regardless of the volume added. Germination of nonprimed seeds was 83% and 2% at 15 and 30C, respectively. Final germination at 30C increased to >80% when seeds were primed with 3 or 4 ml NaOCl solution or 3 ml water for >10 days. SMP treatment significantly reduced the negative effect of high temperature on celery seed germination.

scholarly journals Osmotic Priming or Chilling Stratification Improves Seed Germination of Purple Coneflower

HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1445-1448 ◽  
Author(s):  
N. Wartidiningsih ◽  
R.L. Geneve ◽  
S.T. Kester
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Osmotic Potential ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Polyethylene Glycol 4000 ◽  
Purple Coneflower ◽  
High Germination

High germination seed lots of purple coneflower [Echinacea purpurea (L.) Moench] were evaluated for laboratory germination following osmotic priming or chilling stratification. Compared to nontreated seeds, osmotic priming at 25C in salts (KNO3 + K3PO4; 1:1, w/w) or polyethylene glycol 4000 (PEG) increased early (3-day) germination percentage at 27C of all seed lots, and improved total (10-day) germination percentage of low-germination seed lots. Total germination percentage was unaffected or increased by priming for 4 days compared to 8 days, and by priming at –1.0 MPa compared to –0.5 MPa (except for one low-germination seed lot). Chilling stratification in water at 5 or 10C increased early and total germination of all seed lots, except for that same lot, compared to nontreated seeds. Total germination percentage was unaffected or increased by stratification at 10C rather than at 5C. Neither extending stratification ≥20 days nor lowering osmotic potential with PEG during stratification improved total germination percentage.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Seed Germination and Initial Growth of Pepper Seedlings is Influenced by Environment Temperature and Priming Method

2020 ◽  
Vol 8 (4) ◽  
pp. 540
Author(s):  
Glenda Sallaku ◽  
Gjok Vuksani ◽  
Astrit Balliu
Keyword(s):  
Polyethylene Glycol ◽  
Germination Percentage ◽  
Early Growth ◽  
Distilled Water ◽  
Initial Growth ◽  
Environment Temperature ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Peg 8000 ◽  
Germination Parameters

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.

scholarly journals GERMINATION OF VERBENA SEED IN RESPONSE TO PLANT GROWTH REGULATORS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1156c-1156
Author(s):  
Gamil A. Kabbabe ◽  
William J. Carpenter
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Traditional Method ◽  
Germination Percentage ◽  
Dry Storage ◽  
Gradual Loss ◽  
Acetone Treatment ◽  
Percent Germination ◽  
Peg 8000

Verbena seed when harvested, has a natural dormancy that gradually dissipates during a 5 to 8 month period of dry storage. In this study, the gradual loss of the dormancy causing factor was correlated with germination percentage. Acetone treatment of verbena seeds was found to cause a slight, but non-significant, reduction in total germination. However, the infusion of gibberellic acid (GA4/7) and kinetin (KIN) with the acetone at various concentrations improved germination. The traditional method of seed osmoconditioning using Polyethylene Glycol (PEG 8000) at -1.0 MPa caused a non-significant reduction in percent germination, similar to that with acetone. When growth regulators were mixed with the osmoconditioning solution, at the concentrations used with acetone, a definite and significant improvement in terms of rate and percent of germination was observed.

scholarly journals Priming with Salt Solutions Improves Germination of Pansy Seed at High Temperatures

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 248-250 ◽  
Author(s):  
BeYoung-Han Yoon ◽  
Harvey J. Lang ◽  
B. Greg Cobb
Keyword(s):  
Polyethylene Glycol ◽  
High Temperature ◽  
High Temperatures ◽  
Seedling Emergence ◽  
Salt Solutions ◽  
Percent Germination ◽  
Seed Respiration

Priming regimes were evaluated on pansy seeds (Viola ×wittrockiana Gam. `Crystal Bowl Sky Blue') in high-temperature germination tests. Priming regimes evaluated included CaCl2, MgCl2, K2HPO4, Na2HPO4, NaCl, KCl, KNO3, Na2SO4, and polyethylene glycol 15,000 (PEG15,000) at –1.0 and –2.0 MPa, for 3, 6, or 9 d at 23 °C. Primed and nonprimed control seeds were then germinated at 25, 30, or 35 °C. Total percent germination of nonprimed control seeds was significantly less at 35 °C than at 25 °C. Seeds primed with CaCl2 at –1.0 MPa for 3 d at 23 °C had significantly higher germination at 35 °C than all other priming regimes tested, including aerated PEG8000 at –1.0 MPa for 7 d at 15 °C. Seed respiration, measured by O2 uptake, during germination of seeds primed with CaCl2 was higher than for control seeds or those primed with PEG8000. Priming pansy seed with CaCl2 at –1.0 MPa for 3 d at 23 °C was effective in increasing seedling emergence and for reducing the time of emergence in summer greenhouse studies.

Sign in / Sign up

Export Citation Format

Share Document