scholarly journals Effect of split ejaculation and seminal extenders on longevity of donkey semen preserved at 5° C

2000 ◽  
Vol 52 (4) ◽  
pp. 372-378 ◽  
Author(s):  
S.L.V. Mello ◽  
M. Henry ◽  
M.C. Souza ◽  
S.M.P. Oliveira
Keyword(s):  
Phase Contrast ◽  
Room Temperature ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
The Rich ◽  
Student’S T ◽  
The One ◽  
Artificial Vagina ◽  
Student’S T Test

The aim of this study was to evaluate the longevity of donkey sperm comparing the rich seminal fraction and the whole semen in two extenders, Kenney and modified Baken extenders. Semen of five donkeys were collected through an open-end artificial vagina once a week for five consecutive weeks. The two first jets (rich fraction) of semen were collected separately from the rest of the ejaculate. Whole semen samples were obtained mixing proportionally part of the rich with part of the poor seminal fractions. Seminal samples were immediately diluted 1:1 in each extender and maintained at room temperature during sperm concentration analysis. Samples were further diluted to rich 50×10(6) sperm per ml, cooled in a refrigerator at the initial rate of -0.6° C/min and preserved at 5° C. Total motility (TM), progressive motility (PM) and sperm vigor (V) were examined after final dilution and cooling, and every 24 hours up to the decrease of total motility under 10%. Sperm morphology was evaluated using a phase contrast microscope directly after dilution, on days 3, 6 and 9 post collection. It was used a 2×2 factorial design in a randomised bloc experiment, and means were compared by Student’s t test. Longevity did not vary between the rich seminal fraction and the whole semen for both extenders used. TM, PM, V and sperm morphology were better preserved in the extender with egg yolk (modified Baken extender) than in the one with skimmed milk (Kenney) in both seminal fractions.

scholarly journals 255 PRONUCLEUS FORMATION IN RAT ZONA-FREE OOCYTES CO-CULTURED WITH HOMOLOGOUS POST-THAW SPERMATOZOA

2005 ◽  
Vol 17 (2) ◽  
pp. 277
Author(s):  
Y. Seita ◽  
Y. Okuda ◽  
A. Takizawa ◽  
N. Hirahara ◽  
M. Koichi ◽  
...  
Keyword(s):  
Cooling Rate ◽  
Sperm Motility ◽  
Phase Contrast ◽  
Room Temperature ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Student’S T ◽  
Percentage Data ◽  
Student’S T Test

The aim of the present study was to develop an IVF system with frozen/thawed rat spermatozoa. We examined the effect of cooling rate to 5.0°C on post-thaw sperm motility and membrane integrity, and also investigated the ability of post-thaw spermatozoa to form pronuclei. Under room temperature, epididymal spermatozoa of Wistar rats were collected in 2.0 mL of egg yolk medium containing 8.0% (w/v) lactose monohydrate and 0.7% (v/v) Equex Stem. Samples were loaded into 0.25-mL straws and cooled to 5.0°C in the chamber of a programmed freezer. For cryopreservation, the samples were exposed to liquid nitrogen (LN) vapor for 10 min and then plunged into LN. Straws were thawed in a 37.0°C water bath for 10 s. Ovulated oocytes were collected and the zona pellucidae were removed with 0.1% pronase. One-hundred μL of thawed samples were put into a droplet of 400 μL R1ECM and pre-incubated for 1 h. R1ECM solution was added to the droplet to adjust to 0.5–1.5 × 106 sperm mL−1. The zona-free oocytes were then transferred into the droplet and co-cultured for 10 h. Oocytes were observed for pronuclei formation by means of an inverted phase contrast microscope. In Experiment I, the influence of sperm cooling rate to 5.0°C on sperm motility and membrane integrity was evaluated. Portions of samples were cooled at 54.0°C/min, 0.9°C/min, 0.5°C/min, and 0.3°C/min. The remainders were then frozen. The non-cooled samples were designated as controls. In Experiment II, we examined whether post-thaw spermatozoa have the ability to form pronuclei in vitro or not. All percentage data were arc-sine transformed and then analyzed by the Student's t-test. In Experiment I, the membrane integrity between the spermatozoa cooled at 0.5°C/min and the non-cooled spermatozoa was not different (38.1% vs. 37.2%; P > 0.05), but the integrity of these was higher than in spermatozoa cooled directly at 54.0°C/min (38.1% vs. 25.3%; P < 0.05). After culture for 1 h, the motility of spermatozoa cooled at 0.5°C/min was higher than that of those cooled at 54.0°C/min (61.3% vs. 53.3%; P < 0.05). At 2 h post-thaw the motility of spermatozoa cooled at 0.5°C/min was higher than that of spermatozoa cooled at 54.0°C/min and at 0.9°C/min (11.0% vs. 4.5%, 4.9%; P < 0.05). The membrane integrity of post-thaw spermatozoa cooled at 0.5°C/min was also higher compared to that of spermatozoa cooled at 54.0°C/min (22.5% vs. 8.4%; P < 0.01). In Experiment II, 28 (26.2%) of 107 oocytes had pronuclei when the post-thaw spermatozoa cooled at 0.5°C/min were used. The results indicated that the frozen/thawed spermatozoa cooled to 5.0°C at 0.5°C/min showed higher sperm motility and membrane integrity, and that spermatozoa can form pronuclei in homologous zona-free oocytes in vitro. Although in the rat sperm damage occurred during cooling to 5.0°C, and sperm motility and membrane integrity were also decreased by the cold shock, it is possible to decrease the damage by cooling slowly to 5.0°C at 0.5°C/min.

77 ADDITION OF CHOLESTEROL IN FRESH GOAT SPERM IMPROVES CRYOSURVIVAL RATES

2013 ◽  
Vol 25 (1) ◽  
pp. 186
Author(s):  
B. G. Silva ◽  
E. A. Moraes ◽  
C. S. Oliveira ◽  
W. D. Ferrari Junior ◽  
W. C. G. Matos ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Phase Contrast ◽  
Room Temperature ◽  
Petri Dish ◽  
Egg Yolk ◽  
Irreversible Damage ◽  
Hypoosmotic Swelling Test ◽  
Contrast Microscopy ◽  
Working Solution

Cryopreservation causes irreversible damage to goat sperm membranes, measured by a loss of motile and functional normal cells, compared with fresh sperm. The objective of this study was to determine if the addition of cholesterol-loaded cyclodextrin (CLC) to goat semen improved sperm cryosurvival. The CLC was prepared as described by Purdy and Graham (2004 Cryobiology 48, 36–45) with some modifications: 200 mg of cholesterol were dissolved in 1 mL of chloroform and 1 g of methyl-beta-cyclodextrin was dissolved in 2 mL of methanol. A 0.45-mL aliquot of the cholesterol solution was added to the cyclodextrin solution, after which the mixture was poured into a glass Petri dish and the solvents allowed to evaporate on a warm plate for 24 h. The resulting crystals were removed from the dish and stored at 22°C. A working solution of the CLC was prepared by adding 50 mg of CLC to 1 mL TALP at 37°C. Thirty ejaculates from 5 bucks were collected, diluted 1 : 1 in Tris diluent, divided into 7 equal aliquots, and centrifuged at 800g for 10 min. The sperm pellets were resuspended in Tris diluent, to which 0, 0.75, 1.5, 3.0, 4.5, 6.0, or 7.5 mg of CLC/120 million sperm were added. All treatments were incubated for 15 min at room temperature and then cooled to 4°C over 2 h. The samples were then diluted with Tris-egg-yolk diluent containing 2% glycerol, and the sperm were packaged into 0.5-mL straws, frozen in static liquid-nitrogen vapour for 20 min, and plunged into liquid nitrogen. Straws were thawed in 37°C water for 30 s, extended in Tris, and analyzed using optic microscopy. To test thermal resistance, after thawing, 0.5 mL of semen from each treatment were placed in 1.5-mL Eppendorf tubes in a water bath at 37°C for 3 h. At 0, 60, 120, and 180 min, subsamples were evaluated for sperm progressive motility. A hyposmotic test was also conducted by adding 10 µL of sperm to 2 mL of each solution and incubating them for 1 h/37°C. Sequentially, 20 µL of sperm was diluted in hypoosmotic solution (150 mOsm), and the samples were evaluated using phase-contrast microscopy. A total of 100 spermatozoa were counted in at least 5 different fields, and sperm tails were classified as either noncoiled or coiled. Data were analyzed using ANOVA, and treatment means were separated, using the SNK test at 5% probability. The sperm motility (50.4, 33.8, and 22.5%) was significantly higher for sperm treated with 0.75 mg of cholesterol after 0, 60, and 120 min of incubation after thawing, when compared with other treatments. No treatment differences in the hypoosmotic swelling test were observed. The addition of 0.75 mg of cholesterol to fresh goat semen improved sperm motility after cryopreservation for up to 3 h. Supported by FACEPE and CAPES.

scholarly journals Artifact induction by endodontic materials

2019 ◽  
Author(s):  
Fernanda Cristina Sales Salineiro ◽  
Igor Publio Talamoni ◽  
Solange Kobayashi Velasco ◽  
Fabiana Mesquita Barros ◽  
Marcelo De Gusmão Paraíso Cavalcanti
Keyword(s):  
Region Of Interest ◽  
T Test ◽  
Control Group ◽  
Distribution Analysis ◽  
Subjective Analysis ◽  
Gutta Percha ◽  
Student’S T ◽  
The One ◽  
Student’S T Test

Metallic objects, such as intracanal posts and restorations, may produce severe interference, thus diminishing the quality of CBCT imaging. Objective: The purpose of this study was to analyze the influence of conventional and bioceramic gutta-percha points on the production of artifacts in CBCT images. Methods: Extracted single- -rooted premolar teeth (n=20) were instrumented and scanned with a CBCT device to create three groups: the Control group, the Gutta-Percha group and the Bioceramic Gutta-Percha group. Two types of analysis were executed: an objective one, using the Region of Interest (ROI) to measure the pixel density of each tooth, and a subjective one, to compare the groups’ images. For the statistical analysis, Student’s t-test, descriptive statistics and the frequency distribution analysis were used for both objective and subjective analyses. Results: The agreement between the observers ranged from moderate to excellent. Similar grayscale values were obtained in both the GP and BCGP groups. These results were endorsed by the p-values obtained with Student’s t test. For the subjective analysis, the observers indicated the BCGP group as the one that developed the highest number of artifacts. Conclusions: Both materials produced artifacts in the CBCT images. However, in the subjective analysis, the BCGP group showed higher levels of artifact production than the GP group, which could result in the misdiagnosis of root fracture and in a worse prognosis for that tooth.

scholarly journals FREEZING CAPABILITY OF PASUNDAN BULL SPERM USING TRIS-EGG YOLK, TRIS-SOY, AND ANDROMED® DILUENTS

2017 ◽  
Vol 11 (1) ◽  
pp. 45-49
Author(s):  
Abdullah Baharum ◽  
R. Iis Arifiantini ◽  
Tuty Laswardi Yusuf
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Freezing Process ◽  
Sperm Abnormalities ◽  
Bull Sperm ◽  
Motility Of Sperm ◽  
Artificial Vagina

The aims of this study were to investigate the freezing capability of Pasundan bull spermatozoa in Tris-egg yolk (TEY), Tris-soy (TS), and AndroMed® as diluents. Semen were collected twice a week from four Pasundan bulls aged 3-5 years old using an artificial vagina and evaluated macro- and microscopically. Semen had ≥70% sperm motility, ≥800x106/mL sperm concentration, and less than 20% sperm abnormalities were divided into three parts and each of them diluted with TEY, TS, or AndroMed®. After an equilibration step at 5°C for four hours, diluted semen were packaged in 0.25 mL straw, frozen in liquid nitrogen for ten minutes and kept in liquid nitrogen container until examination. Motility test on fresh, diluted, equilibrated, and after-thawed semen was done using Androvision®. The results showed that after thawing motility of sperm diluted in AndroMed® (58.64±0.72%) was higher than in TEY (49.45±1.22%) and TS (39.34±6.33%). Sperm motility of Pasundan bulls diluted in these three diluents reduced around 33.27±2.45% during freezing process.

scholarly journals 289EFFECT OF FERTILIZATION TIME OF PIG OOCYTES MATURED IN-VITRO BY BOAR SPERM STORED AT 4°C

2004 ◽  
Vol 16 (2) ◽  
pp. 264
Author(s):  
Y.J. Yi ◽  
M.Y. Kim ◽  
Y.J. Chang ◽  
D.I. Jin ◽  
C.S. Park
Keyword(s):  
In Vitro Fertilization ◽  
Room Temperature ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Penicillin G ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Boar Sperm ◽  
Sperm Penetration

The use of boar sperm stored at 4°C may be a useful tool for in vitro production of pig embryos. Therefore, this study was undertaken to investigate the effect of fertilization time of pig oocytes matured in-vitro by boar sperm. The sperm-rich fraction (30–60mL) was slowly cooled to room temperature (20–23°C) by 2h after collection. Semen was transferred into 15-mL tubes, centrifuged at room temperature for 10min at 800g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5mL of the LEN (11.0g lactose hydrate, 20mL egg yolk, 0.05g N-acetyl-D-glucosamine and 100mL distilled water) diluent to provide 1.0×109 spermmL−1 at room temperature. The resuspended semen was cooled in a refrigerator to 4°C. The medium used for oocyte maturation was TCM-199 supplemented with 26.19mM sodium bicarbonate, 0.9mM sodium pyruvate, 10μgmL−1 insulin, 2μgmL−1 vitamin B12, 25mM HEPES, 10μgmL−1 bovine apotransferrin, 150μM cysteamine, 10IUmL−1 PMSG, 10IUmL−1 hCG, 10ngmL−1 EGF, 0.4% BSA, 75μgmL−1 sodium penicillin G, 50μgmL−1 streptomycin sulfate and 10% pFF. After about 22h of maturation, oocytes were cultured without cysteamine and hormones for 22h at 38.5°C, 5% CO2 in air. Oocytes were inseminated with boar sperm stored at 4°C for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9h in 500μL TBM fertilization media with 1×106mL−1 sperm concentration. Thereafter, oocytes were transferred into 500μL NCSU-23 culture medium containing 0.4% BSA for further culture of 6, 48 and 144h, fixed and stained for the evaluation of fertilization parameters and developmental ability. Data were analysed by ANOVA and Duncan’s multiple range test using the SAS program. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times of 6 and 9h than in those of 1 and 3h. The percentage of polyspermic oocytes was highest in fertilization time of 9h compared with other incubation times. The rates of cleaved oocytes were higher in the fertilization times of 6 and 9h (85.0 and 84.6%) compared with those of 1 and 3h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time of 6h (33.6%) than in that of 1, 3 and 9h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9±3.3, 27.6±2.7, 26.3±2.2 and 24.4±1.8 in the fertilization times of 6, 9, 3 and 1h, respectively. In conclusion, we found out that boar sperm stored at 4°C could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend the coincubation time of 6h in 500μL TBM fertilization medium with 1×106mL−1 sperm concentration for in vitro fertilization of pig oocytes matured in vitro.

scholarly journals Preservation of liquid semen and Artificial Insemination in native sheep

1970 ◽  
Vol 7 (2) ◽  
pp. 305-308 ◽  
Author(s):  
S Pervage ◽  
MR Hassan ◽  
M Ershaduzzaman ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Conception Rate ◽  
Abnormal Sperm ◽  
Semen Volume ◽  
Native Sheep ◽  
Artificial Vagina

An experiment was undertaken to determine the conception rate of native sheep by using Artificial Insemination with liquid ram semen. The semen was collected from ram using artificial vagina and the was stored in a refrigerator (4°C) for three days. The volume of semen was extended with egg yolk citrate diluter. A total of 63 ewes were inseminated with stored liquid semen collected from 15 rams by AV method. The total number of spermatozoa, live-dead, normal-abnormal, sperm motility and the pH was observed regularly. The average semen volume per ejaculate was 0.76-1.00ml and the sperm concentration was 2.37x109 - 4.30x109 per ejaculate. The number of normal spermatozoa and the pH was almost similar irrespective of days of storage. Number of live spermatozoa and the sperm motility were reduced with the increasing age of semen. The average conception rate (%) was obtained as 63.61, 61.90, 52.38 and 47.61 with sperm in zero, 1st, 2nd and 3rd day storage respectively. Keywords: Preservation; Liquid semen; Quality; Artificial insemination; Sheep DOI: 10.3329/jbau.v7i2.4739 J. Bangladesh Agril. Univ. 7(2): 305-308, 2009

scholarly journals Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol

2005 ◽  
Vol 57 (6) ◽  
pp. 764-771
Author(s):  
A.R. Silva ◽  
R.C.S. Cardoso ◽  
L.D.M. Silva
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Tris Buffer ◽  
Normal Sperm ◽  
Manual Stimulation

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were observed between treatments after thawing regarding sperm motility and vigor, normal sperm morphology rate or longevity. Both dilution rates can be efficiently used for canine semen freezing.

224 A COMPARATIVE STUDY BETWEEN WOOD AND PLAINS BISON

2008 ◽  
Vol 20 (1) ◽  
pp. 191
Author(s):  
C. Lessard ◽  
J. Danielson ◽  
J. Thundatil ◽  
M. Woodbury ◽  
R. McCorkell
Keyword(s):  
Egg Yolk ◽  
Reproductive Technologies ◽  
Bison Bison ◽  
Animal Origin ◽  
Accessory Glands ◽  
The Status ◽  
Reproductive Techniques ◽  
Wood Bison ◽  
Student’S T ◽  
Student’S T Test

In Canada, brucellosis and tuberculosis threaten an estimated 4500 wood bison (Bison bison athabascae), a species considered at risk by the Committee on the Status of Endangered Wildlife In Canada (COSEWIC). To help rescue this species, our Wood Bison Reproductive Research group proposes to employ advanced reproductive technologies. Unfortunately, little is known about the reproductive physiology of the wood bison, which hinders the application of these reproductive technologies. In order to modify advanced reproductive techniques developed in cattle for use in wood bison, the large amounts of semen, embryos, and oocytes from wood bison required are not available. The purpose of this study was to compare semen collected from the more abundant and closely related plains bison (Bison bison bison) with that of wood bison. Semen from 3 wood and 4 plains bison were collected by electro-ejaculation during the summer of 2007. Andrological parameters of morphology and motility were recorded on fresh semen, extended semen, and post-thawed semen samples. A Student's t-test was used to compare the results of these two groups. Semen was cryopreserved using two commercially available cryopreservation media (Andromed and Triladyl, Minitube Canada, Ingersoll, Ontario, Canada). Sperm morphology and motility were not different between electro-ejaculated samples from plains and wood bison (P > 0.05). Also, no difference was found in the survival rate of sperm from the electro-ejaculated samples between plains and wood bison after freezing and then thawing using an egg-yolk based extender (Triladyl) or an extender containing no products of animal origin (Andromed). A difference between cryopreservation media was found; post-thaw motility of Triladyl-treated sperm was higher (29%) than that of the Andromed-treated sperm (12%). Due to lack of previous success with preserving electro-ejaculated semen in media free of animal-origin products, motility assays were performed to evaluate if spermatozoa retrieved from epididymides of plains bison can be cryopreserved in Andromed. Interestingly, cyropreserved epididymal spermatozoa had a higher motility than cryopreserved electro-ejaculated sperm after freezing-thawing procedures using a medium containing no products of source animal (respectively, 30% v. 7%). This result suggests that there may be a factor secreted by the reproductive accessory glands that interferes with the post-thaw survivability of bison sperm. In conclusion, this study supports the hypothesis that semen from plains bison behaves similarly to that of wood bison semen during cryopreservation and therefore could be used to establish protocols for advanced reproductive technologies in wood bison. This project was supported by Canadian Adaptation and Rural Development in Saskatchewan.

scholarly journals Effect of Polymerization Cycles on Gloss, Roughness, Hardness and Impact Strength of Acrylic Resins

2016 ◽  
Vol 27 (2) ◽  
pp. 176-180 ◽  
Author(s):  
Rafael Leonardo Xediek Consani ◽  
Bianca L. Folli ◽  
Moises C. F. Nogueira ◽  
Americo Bortolazzo Correr ◽  
Marcelo F. Mesquita
Keyword(s):  
Surface Roughness ◽  
Impact Strength ◽  
Room Temperature ◽  
Knoop Hardness ◽  
T Test ◽  
Liquid Ratio ◽  
Acrylic Resins ◽  
Significant Difference ◽  
Student’S T ◽  
Student’S T Test

Abstract The aim of this study was to evaluate the conventional and boiled polymerization cycles on gloss, roughness, hardness and impact strength of acrylic resins. Samples were made for each Classico and QC-20 materials (n=10) in dental stone molds obtained from rectangular metallic matrices embedded in metallic flasks. The powder-liquid ratio and manipulation of the acrylic resins' were accomplished according to manufacturers' instructions and the resins were conventionally packed in metallic flasks. After polymerization by (1) conventional: 74 °C for 9 h (Classico) and (2) boiled: 20 min (QC-20) cycles, the samples were deflasked after cooling at room temperature and conventionally finished and polished. The properties were evaluated after storage in water at 37 °C for 24 h. Gloss was verified with Multi Gloss 268 meter (Konica Minolta), surface roughness was measured with Surfcorder SE 1700 rugosimeter (Kosaka), Knoop hardness number was obtained with HMV-200 microdurometer, and impact strength was measured in an Otto Wolpert-Werke device by Charpy system (40 kpcm). Data were subjected to Student's t-test (at α=0.05). The results were: Gloss: 67.7 and 62.2 for Classico and QC-20 resins, respectively; Surface roughness: 0.874 and 1.469 Ra-µm for Classico and QC-20, respectively; Knoop hardness: 27.4 and 26.9 for Classico and QC-20, respectively; and Impact strength: 37.6 and 33.6 kgf/cm2 for Classico and QC-20, respectively. No statistically significant difference (p>0.05)were found between the resins for the evaluated properties. In conclusion, conventional and boiled polymerization cycles had similar effects on gloss, roughness, hardness and impact strength of both Classico and QC-20 resins.

scholarly journals Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

2021 ◽  
Vol 16 (4) ◽  
pp. 340-346
Author(s):  
R. R. Dapawole ◽  
I. P. Sirappa
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Progressive Motility ◽  
Randomized Design ◽  
Effect Concentration ◽  
Completely Randomized Design ◽  
Artificial Vagina ◽  
Moringa Oleifera Lam

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

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