Obesity and abdominal wound healing in rats

INTRODUCTION: Treatment for obesity essentially has to do with weight loss, which can be achieved through surgical procedures. Despite the considerable rise in the number of such procedures, the relationship between obesity and the healing process has not been totally clarified. PURPOSE: To investigate abdominal wound healing in obese Wistar rats on the seventh and fourteenth days following a laparotomy. METHODS: Thirty-six Wistar rats were randomly distributed into two groups, the control and experiment group. The control group were fed on either a standard diet for the species and the experiment group were put on a high calorie diet. After 116 days, all the animals were submitted to a laparotomy followed by laparorrhaphy. After euthanasia on the seventh or fourteenth day, fragments of the abdominal wound containing the scar were submitted to histopathological and tensiometric analysis. RESULTS: The average weight of the animals from the experiment group was higher than that of the control group (p<0.001). The difference in the resistance of the cutaneous scars was not significant. The aponeurotic scars were more resistant in the control group after seven days (p=0.011) and fourteen days (p=0.040). There was no difference in terms of intensity of the inflammatory reaction and the collagen density was similar in both groups. CONCLUSION: In rats, obesity lowered the resistance of the aponeurotic scars but not the skin scars. It did not interfere with the delayed inflammatory response and the collagen density.

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PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

ODONTO Dental Journal ◽

10.30659/odj.2.2.41-46 ◽

2015 ◽

Vol 2 (1) ◽

pp. 41

Author(s):

Umi Kulsum ◽

Ratnawati Hendari ◽

Siti Chumaeroh

Keyword(s):

Wound Healing ◽

Ulcer Healing ◽

Wistar Rats ◽

Povidone Iodine ◽

Healing Process ◽

Aloe Vera ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

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The Effect of Aloe Vera Extract to Extensive Lesion and Expression of Transforming Growth Factor (TGF-β) on Alkaline Chemical Trauma Cornea Model

Sriwijaya Journal of Ophthalmology ◽

10.37275/sjo.v1i2.36 ◽

2018 ◽

Vol 1 (2) ◽

pp. 1-17

Author(s):

Fahcreza ◽

Elsa Iskandar ◽

Rachmat Hidayat ◽

Petty Purwanita ◽

Anang Tribowo ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Wistar Rats ◽

Aloe Vera ◽

Control Group ◽

Treatment Groups ◽

White Rats ◽

Significant Difference ◽

The Difference ◽

In Cells

Abstract Background: Chemical trauma to the cornea is an emergency condition of the eye that requires early diagnosis and good treatment. Alkaline have ability to saponify fatty acids in cells and cell membranes which can make penetration into the stroma and destroy proteoglycans and collagen in cells. Aloe vera (AV) contains several active substances that are reported to have anti-inflammatory, immunomodulatory, and wound healing effects. AV has been reported to accelerate the healing process of corneal epithelial defects by increasing fibroblast proliferation, collagen production and growth factor production. This study aims to determine the difference between the effect of aloe vera extract with a concentration of 10%, 20%, 40% and BSS on the healing of extensive corneal lesions in white wistar rats alkaline trauma models. Method: This study was an experimental study with a pre and posttest only with control group design in vivo approach to 30 Wistar white rats which were divided into 5 treatment groups for 3 days. Comparative analysis of effectiveness using the ANNOVA test or the Kruskal Wallis test and continued by the post hoc test. Results: Based on the one way ANOVA test there was a statistically significant difference in effectiveness between the five treatment groups on the percentage of corneal wound healing area and TGF-β expression with an assessment of p = 0,000 each. The administration of alloevera (AV) concentration of 20% had a significant difference in percentage of healing of corneal lesions and TGF-β expression compared with other treatment groups with p = 0,000 each. Large differences in the area of corneal lesions in the 40% AV group were -0.45 in the BBS group, 0.146 in the 10% AV group, 0.493 in the 20% AV group. The difference in the AV group 10% was 0.30 in the BBS group, -064 in the AV group 20%, and -0.14 in the AV group 40%. However, TGFβ expression in the normal control group that did not receive treatment was 54.94 (53.21-56-12). TGFβ levels in the BSS group were 10.44, the 10% aloe vera group was 25.43, 47.99 for the 20% aloe vera group and 37.95 for the 40% aloe vera group. Conclusion: There is a difference between the effect of aloe vera extract with concentrations of 10%, 20%, 40% and BSS on the extensive healing of corneal lesions in white wistar rats with alkaline chemical trauma models.

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EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽

10.30649/denta.v12i2.170 ◽

2019 ◽

Vol 12 (2) ◽

pp. 8

Author(s):

Isidora Karsini ◽

Farhana Nur Fadhila ◽

Nafiah Nafiah

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Healing Process ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Unsaturated Fat ◽

Post Test ◽

Channa Striata ◽

The Mean

Background: Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. Purpose : To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. Material and Method : The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). Results : There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). Conclusion : The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.

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Effect of Photobiomodulation Therapy on Oxidative Stress Markers in Healing Dynamics of Diabetic Neuropathic Wounds in Wistar Rats

Cell Biochemistry and Biophysics ◽

10.1007/s12013-021-01021-9 ◽

2021 ◽

Author(s):

Gagana Karkada ◽

G. Arun Maiya ◽

Praveen Arany ◽

Mohandas Rao ◽

Shalini Adiga ◽

...

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Wistar Rats ◽

Healing Process ◽

Photobiomodulation Therapy ◽

Control Group ◽

Sciatic Nerve Crush ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Experimental Group

Abstract Background Prolonged and overlapping phases of wound healing in diabetes are mainly due to the redox imbalance resulting in the chronicity of the wound. Photobiomodulation therapy works on the principle of absorption of photon energy and its transduction into a biological response in the living tissue. It alleviates the cellular responses, thereby improving the mechanism of wound healing in diabetes. Objective To find out the effect of photobiomodulation therapy of dosage 4 J/cm2 in the healing dynamics of diabetic neuropathic wounds in Wistar rats and its relation with oxidative stress markers. Methodology Diabetes was induced using Streptozotocin of 60 mg/kg of body weight to eighteen female Wistar rats. Neuropathy was induced by the sciatic nerve crush injury followed by an excisional wound of 2 cm2 on the back of the animal. Experimental group animals were treated with dosage 4 J/cm2 of wavelength 655 and 808 nm, and control group animals were kept unirradiated. The biomechanical, histopathological, and biochemical changes were analysed in both groups. Results There was a reduction in mean wound healing time and an increased rate of wound contraction in the experimental group animals compared to its control group. The experimental group showed improved redox status, and histopathological findings revealed better proliferative cells, keratinisation, and epithelialization than un-irradiated controls. Conclusions Photobiomodulation therapy of dosage 4 J/cm2 enhanced the overall wound healing dynamics of the diabetes-induced neuropathic wound and optimised the oxidative status of the wound, thereby facilitating a faster healing process.

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The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.37026 ◽

2019 ◽

Vol 1 (1) ◽

pp. 15

Author(s):

Afryla Femilian ◽

Dewi Agustina ◽

Goeno Subagyo

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Buccal Mucosa ◽

Wistar Rats ◽

Carica Papaya ◽

Healing Process ◽

Herbal Medicines ◽

Ethanolic Extract ◽

Control Group ◽

Wound Healing Process

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

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THE EFFECT OF COMBINATION CALCIUM HYDROXIDE-PROPOLIS APPLICATION TO AMOUNT OF NEUTROFIL CELLS ON WISTAR RAT PULP PERFORATION

Conservative Dentistry Journal ◽

10.20473/cdj.v10i2.2020.66-70 ◽

2020 ◽

Vol 10 (2) ◽

pp. 66

Author(s):

Ira Widjiastuti ◽

Setyabudi Setyabudi ◽

Nia Nur Haliza

Keyword(s):

Calcium Hydroxide ◽

Wistar Rats ◽

Healing Process ◽

Wistar Rat ◽

Combination Group ◽

Control Group ◽

Pulp Capping ◽

Pulp Inflammation ◽

Significant Difference ◽

The Difference

Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold standard. Pulp capping by calcium hydroxide could causing tissue necrosis followed by inflammation. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the Nf-kB pathway and pro-inflammatory cytokines. That process will inhibit the increase of vascular permeability and neutrophil chemotaxis. Inflammatory process can accelerate by controlling the amount of neutrophil cells as a acute phase marker, so the healing process occurs more quickly. Therefore it is necessary to study the amount of neutrophil cells in the pulp perforation of Wistar rats after applicated by combination of calcium hydroxide-propolis. Purpose: To analyze the difference amount of neutrophil cells on the Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest Method: M1 tooth perforated for all groups with three types tratment: no medication (control group), calcium hydroxide-aquadest 1: 1 (P-1 group), and calcium hydroxide-propolis 1: 1.5 (P-2 group) and closed with cention. Observations were made on the 1st, and 3rd day with HE staining. Results: One Way ANOVA shown a significant difference (p <0.05) of the average amount of neutrophil cells between all treatment groups on 1st day (p=0,000) and 3rd day (p=0,000). The amount of neutrophil cells in the calcium hydroxide-propolis (P-2) combination group was lower than control group and calcium hydroxide-aquadest (P-1) group on the 1st and 3rd day. Conclusion: The amount of neutrophil cells after application of calcium hydroxide-propolis is lower than calcium hydroxide-aquadest application.

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Expression of Vascular Endothelial Growth Factor (VEGF) and New Blood Vessels Formation on Wound Incision Post Ropivacaine Administration in Animal Model

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20jul550 ◽

2020 ◽

Vol 5 (7) ◽

pp. 829-836

Author(s):

Andri Subiantoro ◽

Arie Utariani ◽

Imam Susilo

Keyword(s):

Wound Healing ◽

Endothelial Cells ◽

Blood Vessels ◽

Wistar Rats ◽

Healing Process ◽

Critical Factor ◽

Histological Evaluation ◽

Control Group ◽

Wound Healing Process ◽

Impaired Wound Healing

Surgical incision wounds causing clinical pain. Inadequate postoperative pain management resulting in impaired wound healing. A critical factor in wound healing is angiogenesis. VEGF is one of the important factors and regulators of angiogenesis. The aim of this study is to analyze the effect of infiltration ropivacaine on VEGF expression, and the formation of new blood vessels, which is very instrumental in the wound healing process. Methods: Twenty-four Wistar rats were divided into two groups, and then superficial-thickness excisional wounds were created. One was treated by ropivacaine infiltration, and the other was not given ropivacaine infiltration as control group. Each of the groups was divided into two subgroups. Each of the subgroups composed of 6 Wistar rats based on the period of termination: 3rd, 7th day after wounding. Histological evaluation was done to determine the presence of new small blood vessels and the expression of VEGF of capillary endothelial cells. The data collected is then analysed by SPSS. Results: The administration of ropivacaine infiltration on superficial-thickness excisional wounds improved wound healing characterized by neovascularization formed and changed of expression of VEGF within cytoplasm or surface of endothelial cells Conclusion: The administration of ropivacaine infiltration has a beneficial effect on the healing process of superficial Wistar rats.

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Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v49.i3.p125-132 ◽

2016 ◽

Vol 49 (3) ◽

pp. 125

Author(s):

Gunawan Oentaryo ◽

Istiati Istiati ◽

Pratiwi Soesilawati

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Healing Process ◽

Tissue Growth ◽

Control Group ◽

Wound Healing Process ◽

Lower Lip ◽

Treatment Groups ◽

Collagen Secretion ◽

Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

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Effectiveness Cream and Ointment of Spirulina platensis extract against amount of Fibroblas and Wound Area: Study on white rats whose skin is incised

Indonesian Journal of Environmental Management and Sustainability ◽

10.26554/ijems.2020.4.2.39-42 ◽

2020 ◽

Vol 4 (2) ◽

Author(s):

Bimby Irenesia ◽

Renni Yuniarti ◽

Endang Mahati

Keyword(s):

Wound Healing ◽

Spirulina Platensis ◽

Wistar Rats ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Control Group Design ◽

Wound Area ◽

White Rats

Spirulina platensishas been well-known as an abundant protein and phycocyanin. Spirulina platensis has the effect of anti-inflammatory, so it is widely used to apply cosmetics and biomedicine in the healing process of wounds. To prove that the administration of an extract ofSpirulina platensiscan increase the number of fibroblasts and accelerate the wound healing process in Wistar rats with an incision on their skin. This study used a randomizedpost-test only control group design. The skins of thirty (30) male Wistar rats were incised. This treatment was divided into 4 groups, namely the group that received the 0,1% cream (X1) of S.platensisextract, and the group that was administered with the 0,1% ointment (X2) of S.platensis extract; the negative control group was only treated by a saline solution (C1), and the positive control group was with the administration of Gentamycin 0,1% ointment (C2). The total numbers of fibroblasts were examined on day 14 and calculated using the method of tissue biopsy and hematoxylin-eosin (HE) staining. The wound was checked after 14-day Data analysis was performed with a one-wayANOVA test and was continued by Post Hoc Test LSD. The total numbers of fibroblasts on day 14 from the group of XI, X2, C1, and C2 were 15.00; 17,50; 3,83, 10,33 with p<0.001. The average size of the wounds on day 14 from the groups of X1, X2, C1, and C2 was 551,78; 435,76; 1795,95; 1193,57 with p<0.001, respectively. PostHoc Testresults on the total number of fibroblasts and extensive wound area showed significant differences between them. Administration of the 0.1% ointment of Spirulina platensisextract believed to potentially increasing the number of fibroblasts and accelerate the process of wound healing.

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Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel

Infectious Disease Reports ◽

10.4081/idr.2020.8726 ◽

2020 ◽

Author(s):

Muhammad Luthfi ◽

Wisnu Setyari Juliastuti ◽

Yuniar Aliyah Risky ◽

Elvina Hasna Wijayanti ◽

Aisyah Ekasari Rachmawati ◽

...

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Wistar Rats ◽

Tooth Extraction ◽

Healing Process ◽

Wistar Rat ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Fruit Extract

Background: Tooth extraction is a dental procedure for removing a teeth from the alveolar bone socket. The tooth extraction process causes damage to hard tissue and soft tissue, so the body will respond physiologically to heal the wound. The wound healing process is divided into several phases, one of which is the proliferation phase of fibroblasts, which is one of the most important phases in the process of wound healing. Okra fruit contains saponins, tannins, flavonoids and alkaloids that have anti-inflammatory, antibacterial, antioxidant effects, and can stimulate angiogenesis so to accelerate the process of wound healing. Objective: to prove that the administration of okra fruit extract can accelerate the process of wound healing after extraction in the teeth of Wistar rats through increased expression of fibroblast cells. Methods: 18 Wistar rats were divided into 2 groups; control group and treatment group. The treatment group received a 30% okra fruit extract. The number of fibroblasts was calculated statistically using One Way ANOVA and Tukey HSD. Results: The results showed that the expression of control group fibroblast cells on day 3 (19.00 ± 2.0), day 5 (21.67 ± 2.08), day 7 (24.00 ± 2.00), whereas in the treatment group on day 3 (24.00 ± 1.00), day 5 (29.00± 2.00), day 7 (30.00 ± 1.53). Anova test between groups showed a significant difference with P-value 0.006. Conclusion: 30% okra fruit extract can increase fibroblast expression in wound healing process after extraction of Wistar rat teeth.

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