scholarly journals Antioxidant activity of Spathodea campanulata (Bignoneaceae) extracts

2012 ◽  
Vol 14 (2) ◽  
pp. 287-292 ◽  
Author(s):  
S.C Heim ◽  
F.A Guarnier ◽  
D.T Ferreira ◽  
R Braz-Filho ◽  
R Cecchini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Complex Formation ◽  
Mechanism Of Action ◽  
Iron Complex ◽  
Bark Extract ◽  
Flower Extract ◽  
Antioxidant Mechanism ◽  
Activity Loss ◽  
Ethanol Extracts ◽  
Antioxidant Mechanisms

Spathodea campanulata is used in traditional medicine in Africa as diuretic and anti-inflammatory. Although few studies have reported the mechanism of antioxidant action, this study evidenced the antioxidant activity of S. campanulata bark and flower extracts and their possible mechanism of action. Ethanol extracts of S. campanulata bark and flowers showed antioxidant activity on lipid peroxidation of liver microsome induced by Fe3+-ascorbic acid. Bark extract was 5 times more efficient than flower extract. The antioxidant activity of flower extract, previously complexed with increasing concentrations of Fe3+ (20 - 100 μM) which resulted in antioxidant activity loss, was shown to be related to iron complex formation. In contrast, the antioxidant activity of bark extract was not inhibited by the previous incubation with Fe3+, although complexation was demonstrated by spectral analysis of the solution. These results suggest an antioxidant mechanism other than Fe3+ complex formation. Therefore, the antioxidant mechanisms of S. campanulata flower and bark extracts are distinct from each other, reflecting the extract heterogeneous composition and the mechanism of action.

scholarly journals In-vitro antifungal activity of leaf, stem bark and root extracts of Irvingia gabonensis against fungi associated with its seed deterioration

2021 ◽  
Vol 23 (2) ◽  
pp. 135-146
Author(s):  
I. T. Gbadamosi ◽  
O. T. Obanubi ◽  
A. A. Sobowale
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Stem Bark ◽  
Free Radical Scavengers ◽  
Bark Extract ◽  
Irvingia Gabonensis ◽  
Model Treatment ◽  
Ethanol Extracts ◽  
Spoilage Fungi

Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill seeds are often contaminated in storage by postharvest spoilage fungi. This study investigated the nutritional contents of the seeds, phytochemical contents and antioxidant activity of different parts of the plant as well as their antifungal effects against fungi isolated from the deteriorated seeds. Proximate, mineral and phytochemical analyses were carried out using standard laboratory techniques. The antioxidant activity was against DPPH radicals. Ethanol extracts (100 and 200 mg/ml) of the various parts were screened against the isolates using pour plate method in Potato Dextrose Agar (PDA) plates. The plates were examined for growth daily for 10 days. Data were statistically analysed. The healthy seeds contained higher protein (13.73%), fat (58.73%) and fibre (2.50%), whereas, higher content of moisture (10.17%) and carbohydrate (15.47%) were observed in the spoilt seeds. The leaf had highest contents of alkaloids (1228.3 mg/100 g), flavonoids (1345.9 mg/100 g), tannins (1345.9 mg/100 g), saponins (578.3 mg/100 g) and carotenoids (2348.3 μg/100 g). The antioxidant activity of the leaf (50.30%) was the highest. Fungi isolated from the deteriorated seeds were Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Curvilaria lunata. The leaf was most effective against A. niger, A. flavus and C. lunata whereas the stem bark extract was most effective against A. fumigatus. The F-values were significant for model, treatment, concentration and number of days for the leaf and root. This study confirmed the antifungal activity of extracts of various parts of the plant against isolates implicated in the deterioration of its seeds. The extracts could be used as preservative to enhance the shelf-life and nutritional quality of the seeds. Keywords: Irvingia gabonensis, Spoilage of seeds, Antifungal activity, Free radical scavengers, Preservatives

scholarly journals Antioxidant, cytotoxic and UVB-absorbing activity of Maytenus guyanensis Klotzch. (Celastraceae) bark extracts

2006 ◽  
Vol 36 (4) ◽  
pp. 513-518 ◽  
Author(s):  
Patrícia de Almeida Telles Macari ◽  
Cíntia Nicácio Portela ◽  
Adrian Martin Pohlit
Keyword(s):  
Antioxidant Activity ◽  
Brine Shrimp ◽  
Room Temperature ◽  
Quantitative Methods ◽  
Skin Cancer Prevention ◽  
Bark Extract ◽  
Medicinal Tree ◽  
Freeze Dried ◽  
Ethanol Extracts

Maytenus guyanensis Klotzch. is an Amazonian medicinal tree species known in Brazil by the common name chichuá and in Peru and Colombia by the name chuchuhuasi. It is used in traditional medicine as stimulant, tonic, and muscle relaxant, for the relief of arthritis, rheumatism, hemorrhoids, swollen kidney, skin eruptions, and skin cancer prevention, among others. Initially, different extraction solvents and methods were applied to dried, ground bark which made possible the preparation of extracts having both significant lethality to brine shrimp larvae (Artemia franciscana Leach) as well as antioxidant activity in vitro based on tests involving reactions with 2,2,-diphenyl-1-picrylhydrazyl (DPPH). Analysis of fractions from serial extractions with solvents of increasing polarity supports the notion that antioxidant activity is associated with compounds of intermediate polarity and cytotoxicity is associated with compounds of low to intermediate polarity. Variation of extraction time and conditions revealed that hot, continuous ethanol extraction provided good yields of bark extract in several hours. Hot extraction also provided ethanol extracts having greater lethality to brine shrimp and antioxidant activity (compared to the flavonoid rutin in semi-quantitative methods based on DPPH) than extracts obtained from maceration at room temperature. Freeze-dried ethanol extracts were prepared by: 1) maceration at room temperature and 2) hot extraction (eight hours) on several hundred gram scales and the latter extract was shown to have partial screening effects on UVB light. In this work, cytotoxic, antioxidant and potential sun-screening activity are shown for the first time in M. guyanensis.

Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

2018 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Vijaya Jyothi M ◽  
Bhargav E ◽  
Pavan Kumar K ◽  
Praneeth Gowd K ◽  
Ram Pavan S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Total Phenolic ◽  
Standard Drug ◽  
Flower Extract ◽  
Reducing Ability ◽  
Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

scholarly journals Insights into the Antioxidant Mechanism of Newly Synthesized Benzoxazinic Nitrones: In Vitro and In Silico Studies with DPPH Model Radical

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1224
Author(s):  
Stefania Marano ◽  
Cristina Minnelli ◽  
Lorenzo Ripani ◽  
Massimo Marcaccio ◽  
Emiliano Laudadio ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Epr Spectroscopy ◽  
Point Of View ◽  
Uv Spectrophotometry ◽  
Design And Synthesis ◽  
Antioxidant Mechanism ◽  
Primary Mechanism ◽  
In Silico Studies ◽  
Wide Range

Synthetic nitrone spin-traps are being explored as therapeutic agents for the treatment of a wide range of oxidative stress-related pathologies, including but not limited to stroke, cancer, cardiovascular, and neurodegenerative diseases. In this context, increasing efforts are currently being made to the design and synthesis of new nitrone-based compounds with enhanced efficacy. The most researched nitrones are surely the ones related to α-phenyl-tert-butylnitrone (PBN) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) derivatives, which have shown to possess potent biological activity in many experimental animal models. However, more recently, nitrones with a benzoxazinic structure (3-aryl-2H-benzo[1,4]oxazin-N-oxides) have been demonstrated to have superior antioxidant activity compared to PBN. In this study, two new benzoxazinic nitrones bearing an electron-withdrawing methoxycarbonyl group on the benzo moiety (in para and meta positions respect to the nitronyl function) were synthesized. Their in vitro antioxidant activity was evaluated by two cellular-based assays (inhibition of AAPH-induced human erythrocyte hemolysis and cell death in human retinal pigmented epithelium (ARPE-19) cells) and a chemical approach by means of the α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging assay, using both electron paramagnetic resonance (EPR) spectroscopy and UV spectrophotometry. A computational approach was also used to investigate their potential primary mechanism of antioxidant action, as well as to rationalize the effect of functionalization on the nitrones reactivity toward DPPH, chosen as model radical in this study. Further insights were also gathered by exploring the nitrone electrochemical properties via cyclic voltammetry and by studying their kinetic behavior by means of EPR spectroscopy. Results showed that the introduction of an electron-withdrawing group in the phenyl moiety in the para position significantly increased the antioxidant capacity of benzoxazinic nitrones both in cell and cell-free systems. From the mechanistic point of view, the calculated results closely matched the experimental findings, strongly suggesting that the H-atom transfer (HAT) is likely to be the primary mechanism in the DPPH quenching.

scholarly journals Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1325
Author(s):  
Mohamed Z. M. Salem ◽  
Abeer A. Mohamed ◽  
Hayssam M. Ali ◽  
Dunia A. Al Al Farraj
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Leaf Extracts ◽  
Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

scholarly journals SIFAT FISIKOKIMIA HAND AND BODY CREAM DENGAN PEMANFAATAN EKSTRAK ETANOL BUNGA GEMITIR (Tagetes erecta L.) DAN BUNGA PACAR AIR MERAH (Impatiens balsamina L.) DARI LIMBAH CANANG

2017 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Dewa Ayu Ika Paramitha ◽  
James Sibarani ◽  
Ni Made Suaniti
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
National Standard ◽  
Tagetes Erecta ◽  
Basic Formula ◽  
Impatiens Balsamina ◽  
Ethanol Extracts ◽  
Tagetes Erecta L ◽  
Microbial Numbers

ABSTRAK: Pada penelitian ini dilakukan pengembangan formula sediaan hand and body cream dengan memanfaatkan sumber antioksidan yang dimiliki oleh dua jenis ekstrak etanol yaitu ekstrak bunga pacar air merah (Impatiens balsamina L.) dan bunga gemitir (Tagetes erecta L.) dengan taraf perlakuan sebesar 1% dari masing-masing ekstrak (FPM dan FG) dan campuran 0,5% ekstrak pacar air merah dan 0,5% ekstrak gemitir (FCPMG). Pengujian terhadap  produk dilakukan pada sifat fisikokimia  meliputi pH, viskositas, aktivitas antioksidan, angka lempeng total mikroba, dan ukuran partikel. Berdasarkan hasil pengujian produk hand and body cream yang dihasilkan memiliki antioksidan yang cukup tinggi sebagai produk perawatan tubuh dengan penambahan 1% ekstrak etanol pacar air merah, gemitir dan campuran dari kedua ekstrak etanol. Kualitas produk hand and body cream yang dihasilkan baik dan memenuhi standar mutu SNI dari aspek nilai pH, viskositas, dan nilai ALT. Warna yang dihasilkan mulai dari warna kuning kemerahan sampai dengan warna kuning, dan partikel yang dimiliki oleh produk adalah nanopartikel dengan sistem polidispersi. ABSTRACT: Formulations of hand and body creams using ethanol extracts of flowers of red Impatiens balsamina L. And Tagetes erecta L. As antioxidant sources have been developed.  One percent of  extracts (FPM and FG) and the mixture of 0.5% each extracts (FCPMG) were added to the basic formula. The analysis of physicochemical properties of the products was conducted including pH, viscosity, antioxidant activity, the size of the particles, and total microbial plate numbers. Based on the results, the quality of the hand and body creams meet the national standard of Indonesia (SNI) regarding to pH, viscosity, and the total microbial numbers. The colours of the hand and body creams resulted are reddish yellow to yellow with polydispersion nanoparticles.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals Analytical Profiling of Proanthocyanidins from Acacia mearnsii Bark and In Vitro Assessment of Antioxidant and Antidiabetic Potential

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2891 ◽  
Author(s):  
Xiao Chen ◽  
Jia Xiong ◽  
Shenlin Huang ◽  
Xun Li ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Degree Of Polymerization ◽  
Acacia Mearnsii ◽  
Oligomeric Proanthocyanidins ◽  
Normal Phase Hplc ◽  
Inhibitory Activities ◽  
Ethanol Extracts ◽  
Alternative Drugs ◽  
Tof Ms

The proanthocyanidins from ethanol extracts (80%, v/v) of Acacia mearnsii (A. mearnsii) bark on chemical-based and cellular antioxidant activity assays as well as carbolytic enzyme inhibitory activities were studied. About 77% of oligomeric proanthocyanidins in ethanol extracts of A. mearnsii bark were found by using normal-phase HPLC. In addition, HPLC-ESI-TOF/MS and MALDI-TOF/TOF MS analyses indicated that proanthocyanidins from A. mearnsii bark exhibited with a degree of polymerization ranging from 1 to 11. These results of combined antioxidant activity assays, as well as carbolytic enzyme inhibitory activities of proanthocyanidins from A. mearnsii bark, indicated an encouraging antioxidant capacity for the high polyphenol content and a potential for use as alternative drugs for lowering the glycemic response.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

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