Antioxidant ability of polyphenols from black rice, buckwheat and oats: In vitro and in vivo

Grains (black rice, buckwheat and oats) contain polyphenols and have stronger antioxidant capacity than staple foods. Their polyphenols were identified and investigated for their antioxidant capacity. The black rice and buckwheat polyphenols were mainly flavonoids; those in oats were phenolic acids. In vitro, their radical-scavenging capacities were determined as black rice > buckwheat > oats. Similarly, in vivo, the increase in total antioxidant capacities and decline in malondialdehyde indicated the enhancement of radical-scavenging and repair abilities of all polyphenol extracts. Differences in superoxide dismutase, catalase activities, glutathione peroxidase activities and oxidase activities suggested that polyphenols from black rice and buckwheat have higher antioxidant activities, indicating that their antioxidant ability is related to polyphenol composition which depends on a polyphenol source. Thus, a combination of diets will make a complementary mixture of polyphenols that can enhance absorption in the intestinal tract and defence ability against oxidative stress.

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EFFECT OF TEMPERATURES EXTRACTION ON IN VITRO ANTIOXIDANT ACTIVITIES OF POLYSACCHARIDES FROM OPHIOCORDYCEPS SOBOLIFERA

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v128i1d.5273 ◽

2019 ◽

Vol 128 (1D) ◽

Cited By ~ 1

Author(s):

Khoa Van Tran ◽

Hieu Trung Le ◽

Son Lam Le ◽

Vu Xuan Anh Ho ◽

Kiet Tam Trinh ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Extraction Temperature ◽

Total Antioxidant ◽

Value Range ◽

Ophiocordyceps Sobolifera ◽

Dpph Radical Scavenging

The objective of the work reported in this paper was to evaluated effects of temperatures extraction on in vitro antioxidant activities of polysaccharides from the Ophiocordyceps sobolifera. The antioxidant capacity of Ophiocordyceps sobolifera polysaccharide was evaluated to use 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and total antioxidant activity methods. From this study, it could be concluded that the highest antioxidant activity of the polysaccharides could be obtained from extraction temperature 80 oC. Total antioxidant capacity of polysaccharides from Ophiocordyceps sobolifera contained from 0.1183 ± 0.0011 to 0.1454 ± 0.0021 mg GA/g or from 0.1105 ± 0.0004 to 0.1215 ± 0.0006 μmol AS/g. Polysaccharides has the most potential antioxidant activity with the lowest half maximal inhibitory concentration (IC50) value range 0.97 mg/mL to 1.06 mg/mL.

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Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/3043720 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Maria Lúcia da Silva Cordeiro ◽

Ana Raquel Carneiro Ribeiro ◽

Luciana Fentanes Moura de Melo ◽

Lucas Felipe da Silva ◽

Gabriel Pereira Fidelis ◽

...

Keyword(s):

Liquid Chromatography ◽

Antioxidant Capacity ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

In Vitro Assays ◽

Leaf Extracts ◽

In Vivo Assays

Commiphora leptophloeos is widely used in folk medicine without any scientific basis. Considering this, the aim of this study was to evaluate the chemical profile and the antioxidant activity of C. leptophloeos leaf extracts using in vitro and in vivo assays. Six extracts were obtained from fresh leaves using a serial extraction (nonpolar to polar solvents). These extracts were first evaluated with the presence of phytochemical compounds using the methods thin layer chromatography (TLC), ultrahigh performance liquid chromatography (UHPLC-DAD), and high performance liquid chromatography, both with diode array detection (HPLC-DAD). Based on the compounds identified, it was used some bioinformatics tools in order to identify possible pathway and gene targets. After that, the antioxidant capacity from these extracts was analysed by in vitro assays and in vivo assays using Caenorhabditis elegans model. Phytochemical analyses showed the presence of polyphenols, such as rutin, vitexin, and quercetin diglycosides in all extracts, especially in ethanol extract (EE) and methanol extract (EM). Bioinformatics analysis showed these polyphenols linked to antioxidant pathways. Furthermore, EE and EM displayed a high antioxidant capacity in DPPH and superoxide radical scavenging assays. They also had no effect on cell viability for 3T3 nontumour cell. However, for B16-F10 tumour cell lines, these extracts had toxicity effect. In vivo assays using C. elegans N2 showed that EE was not toxic, and it did not affect its viability nor its development. Besides, EE increased worm survival under oxidative stress and reduced intracellular reactive oxygen species (ROS) levels by 50%. Thus, C. leptophloeos EE displayed an important in vitro and in vivo antioxidant capacity. The EE extract has polyphenols, suggesting that these compounds may be responsible for a myriad of biological activities having this potential to be used in various biotechnological applications.

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In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽

10.3390/antiox8080309 ◽

2019 ◽

Vol 8 (8) ◽

pp. 309

Author(s):

Olukayode O. Aremu ◽

Adebola O. Oyedeji ◽

Opeoluwa O. Oyedeji ◽

Benedicta N. Nkeh-Chungag ◽

Constance R. Sewani Rusike

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Methyl Ester ◽

Leaf Extract ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Hypertensive Rats ◽

Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

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Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2015-0049 ◽

2016 ◽

Vol 27 (2) ◽

Cited By ~ 16

Author(s):

Ganiyu Oboh ◽

Veronica O. Odubanjo ◽

Fatai Bello ◽

Ayokunle O. Ademosun ◽

Sunday I. Oyeleye ◽

...

Keyword(s):

Leaf Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Seed Extract ◽

Persea Americana ◽

Dependent Manner ◽

Natural Treatment

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.

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Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem098 ◽

2009 ◽

Vol 6 (2) ◽

pp. 227-231 ◽

Cited By ~ 28

Author(s):

S. A. Adesegun ◽

A. Fajana ◽

C. I. Orabueze ◽

H. A. B. Coker

Keyword(s):

Ascorbic Acid ◽

Leaf Extract ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

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2,5-disubstituted-4-thiazolidinones: Synthesis, anti-inflammatory, free radical scavenging potentials and structural insights through molecular docking

Letters in Organic Chemistry ◽

10.2174/1570178618666210706111055 ◽

2021 ◽

Vol 18 ◽

Author(s):

Jagseer Singh ◽

Pooja A Chawla ◽

Rohit Bhatia ◽

Shamsher Singh

Keyword(s):

Free Radicals ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Assay Method ◽

Acidic Group ◽

Active Compounds ◽

Cox 2 ◽

Anti Inflammatory

: The present work reports synthesis and screening of fifteen 2,5-disubstituted-4-thiazolidinones with different substitutions of varied arylidene groups at imino. The structures of the compounds were confirmed by spectral characterization. The compounds were subjected to in vivo anti-inflammatory and in vitro antioxidant activities. The derivatives possessed remarkable activities quite close to standard drugs used. Unlike conventional non-selective NSAIDs, the synthesized compounds did not contain any acidic group, thereby ensuring a complete cure from ulcers. To further substantiate the claim for safer derivatives, the active compounds were docked against the cyclooxygenase (COX)-2 enzyme. It was found that 4-fluorophenylimino substituent at 2- position and 3-nitro moiety on a 5-benzylidene nucleus of the 4-thiazolidinone derivative fitted in the COX-2 binding pocket. The compounds exhibited remarkable activity in scavenging free radicals, as depicted by the DPPH assay method. The structure-activity relationship was also established in the present work with respect to the nature and position of the substituents. The active compounds were evaluated for drug-like nature under Lipinski’s rule of five, and the toxicity behaviour of active compounds was predicted using ADMETlab software. The compounds have the potential to target degenerative disorders associated with inflammation and the generation of free radicals.

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Antioxidant Activities and Reduced Amyloid-β Toxicity of 7-Hydroxycalamenene Isolated from the Essential Oil of Zelkova serrata Heartwood

Natural Product Communications ◽

10.1177/1934578x1601100943 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1934578X1601100

Author(s):

Pei-Ling Yen ◽

Sen-Sung Cheng ◽

Chia-Cheng Wei ◽

Huan-You Lin ◽

Vivian Hsiu-Chuan Liao ◽

...

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Amyloid Β ◽

Total Phenolic ◽

C Elegans ◽

In Vivo Assays ◽

Zelkova Serrata

The in vitro and in vivo antioxidant activities and its potential to protect against amyloid-β toxicity of essential oils from Zelkova serrata (Thunb.) Makino were investigated in the model organism Caenorhabditis elegans. The results revealed that the essential oil of Z. serrata heartwood exhibited great radical scavenging activities and high total phenolic content. In vivo assays showed significant inhibition of oxidative damage in wild-type C. elegans under juglone-induced oxidative stress and heat shock. Based on results from both in vitro and in vivo assays, the major compound in essential oil of heartwood, (-)-(1 S, 4 S)-7-hydroxycalamenene (1 S, 4 S-7HC), may contribute significantly to the observed antioxidant activity. Further evidence showed that 1 S, 4 S-7HC significantly delayed the paralysis phenotype in amyloid beta-expressing transgenic C. elegans. These findings suggest that 1 S, 4 S-7HC from the essential oil of Z. serrata heartwood has potential as a source for antioxidant or Alzheimer's disease treatment.

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Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

Advances in Polymer Technology ◽

10.1155/2019/8191385 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Ranran Hou ◽

Qiu Li ◽

Jie Liu ◽

Yuanliang Hu

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antioxidant Effect ◽

Ftir Spectrometry ◽

Mda Content ◽

Selenylation Modification ◽

Atractylodes Macrocephala

The Atractylodes macrocephala polysaccharide (AMP) was extracted by water extracting-alcohol precipitating method and further purified by DEAE column. After that, the polysaccharides were modified by nitric acid-sodium selenite method, and nine kinds of selenizing AMPs (sAMPs) were obtained, namely, from sAMP1 to sAMP9. AMP and sAMP were characterized using FTIR spectrometry. Then their antioxidant activities in vitro were measured by free radical-scavenging test. Among these, sAMP6 presented the strongest antioxidant effect. For the test in vivo, the chickens at day 14 vaccinated with ND vaccine were repeatedly vaccinated at day 28. The chickens in sAMP and AMP were injected respectively with 1 mg of sAMP6 and AMP and, in vaccination control (VC) and BC groups, injected with equal volume of normal saline. Respectively, after the first vaccine, on days 7, 14, 21, and 28, the serum GSH-Px and SOD activities and MDA content were determined. The results suggested that sAMP6 could significantly promote GSH-Px and SOD activities and decrease MDA content. All these results indicated that selenylation modification could significantly enhance the antioxidant activity of AMP.

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Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

International Journal of Scientific World ◽

10.14419/ijsw.v3i2.4741 ◽

2015 ◽

Vol 3 (2) ◽

pp. 192 ◽

Cited By ~ 1

Author(s):

Hatil EL-Kamali ◽

Sana E. M. Hamed

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Punica Granatum ◽

In Vitro Models ◽

Water Extracts ◽

Total Antioxidant ◽

Sudanese Medicinal Plants

In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of Punica granatum showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of Commiphora myrrha 58±0.01 and Azadirachta indica 57±0.20 respectively.

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A Review of Recent Studies on the Antioxidant Activities of a Third-Millennium Food: Amaranthus spp.

Antioxidants ◽

10.3390/antiox9121236 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1236

Author(s):

Seon-Joo Park ◽

Anshul Sharma ◽

Hae-Jeung Lee

Keyword(s):

Antioxidant Activity ◽

Bioactive Peptides ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Sustainable Food ◽

Amaranth Species ◽

Amaranthus Spp

Amaranth (Amaranthus spp.) plant commonly refers to the sustainable food crop for the 21st century. The crop has witnessed significant attention in recent years due to its high nutritional value and agronomic advantages. It is a relatively well-balanced cosmopolitan food that is a protector against chronic diseases. Usually, the antioxidant activities of amaranth are held responsible for its defensive behavior. Antioxidant activity of plants, generally, is attributed to their phytochemical compounds. The current interest, however, lies in hydrolysates and bioactive peptides because of their numerous biological functions, including antioxidant effect. While the importance of bioactive peptides has been progressively recognized, an integrated review of recent studies on the antioxidant ability of amaranth species, especially their hydrolysates and peptides has not been generated. Hence, in this review, we summarize studies focused on the antioxidant capacity of amaranth renewal over the period 2015–2020. It starts with a background and overall image of the amaranth-related published reviews. The current research focusing on in vitro, in vivo, and chemical assays-based antioxidant activity of different amaranth species are addressed. Finally, the last segment includes the latest studies concerning free radical scavenging activity and metal chelation capacity of amaranth protein hydrolysates and bioactive peptides.

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