scholarly journals Seed development stages of kale (Brassica oleracea var. acephala L.) genotypes in Turkey

2011 ◽  
Vol 32 (No. 4) ◽  
pp. 147-153 ◽  
Author(s):  
E. Demir ◽  
A. Balkaya

This research determined the stages of kale (Brassica oleracea var. acephala L.) seed development in Samsun province, Turkey, between 2002 and 2003. Plants of inbred kale lines (55TE07, 55TK09, 52PE09, 61ÇY01 and 67DE01) were grown. On the basis of seed and embryo colour, seed morphology (seed length, seed width and embryo length), seed development can be divided into 7 discrete but contiguous stages. The germination rate of inbred lines was at its maximum, or near to its maximum, until the seed moisture declined to 50%. Germination rates reached a maximum level at 65 days after pollination (DAP). It was concluded that kale seeds should be harvested when pods became brown in stage 7 (65 DAP). This study will be used for comparisons with seed development in other Brassica species.  

HortScience ◽  
2020 ◽  
pp. 1-8
Author(s):  
Nebahat Sari ◽  
Emily Silverman ◽  
Danny Reiland ◽  
Todd C. Wehner

Bottle gourd [Lagenaria siceraria (Molina) Standl.] is widely produced in some Asian and African countries as a fresh vegetable as well as for seed consumption. A major use of bottle gourd is for rootstocks in grafted watermelon production. There are several centers where bottle gourd genetic resources are maintained, with the U.S. Department of Agriculture (USDA) germplasm collection being one of the most important. There is little published information on the relationship between seed morphology and the early establishment of seedlings in bottle gourd. The objective of this study was to determine seed characterization, morphology, and cotyledon shape in 163 Lagenaria spp. accessions and measure any relationship between seed and cotyledon characteristics. In USDA Lagenaria germplasm, it has been determined that the common character in seeds was medium in terms of seed size (53% of accessions), intermediate in seed surface lustre (39% of accessions), brown in seedcoat color (89% of accessions), thin and uniform in seed margin (35% of accessions), and tan in seed margin color (64% of accessions). According to the research results, seed weight ranged from 0.11 g (PI 500820) to 0.36 g (PI 675112), seed length from 13.17 mm (PI 500820) to 23.68 mm (PI 675112), and seed width from 5.86 (PI 500808) to 11.21 mm (PI 491274). Cotyledon length ranged from 5.46 cm (PI 368640) to 2.47 cm (PI 381850). The widest cotyledon was 3.00 cm (PI 534552), and the narrowest was 1.50 cm (PI 381831). Interesting correlations were observed for seed weight with seed length (R2 = 0.259), and cotyledon length with cotyledon width (R2 = 0.547).


Phytotaxa ◽  
2020 ◽  
Vol 434 (1) ◽  
pp. 35-64
Author(s):  
FUNDA ÖZBEK ◽  
MEHMET ERKAN UZUNHİSARCIKLI ◽  
MURAT EKİCİ ◽  
EBRU DOĞAN GÜNER ◽  
NUR MÜNEVVER PINAR

73 taxa of the genus Scrophularia (Scrophulariaceae) from Turkey have been investigated using light stereomicroscope (LM) and scanning electron microscopy (SEM) in order to describe their seed morphological characteristics and to evaluate the diagnostic value of these features as a contribution to the genus systematics. The seeds are oblong, ellipsoid, ovoid or obovoid in shape and light or dark brown, blackish-brown to black in colour. The seed length ranges from 0.41 to 2.04 mm and seed width from 0.24 to 1.33 mm. All investigated taxa have bireticulate-alveolate ornamentation with vesicles on the corners of secondary reticulum cells except of S. zuvandica. The alveoli are oblong, orbicular and linear with a size of 7.63–143.1 × 21.62–314.2 µm. Cluster analysis and principal component analysis have been used to determine the contribution of seed morphological characters to the taxa relationships. Numerical analysis has revealed that the seed size and the secondary reticulum properties have significant importance in taxonomy of the genus for distinguishing taxa from each other.


2000 ◽  
Vol 78 (4) ◽  
pp. 481-496 ◽  
Author(s):  
E R Chang ◽  
T A Dickinson ◽  
R L Jefferies

An identification database for seeds in coastal habitats at La Pérouse Bay, Manitoba, Canada, was developed using the DELTA (DEscriptive Language for Taxonomy) format and the program INTKEY. The database should be applicable throughout the coastal lowlands of the Hudson Bay and James Bay regions as similar plant assemblages are present. The database, based upon morphological characteristics and also incorporating ecological information, was used to identify seed specimens found in soil cores and seed traps. The most useful characters for identifying seeds were seed length, seed width, and seed shape. The database is available on the World Wide Web at http://prod.library.utoronto.ca/polyclave/. The seed flora is also documented in the form of a checklist, the first one published for the La Pérouse Bay region; this checklist is also applicable to other coastal areas in the Hudson Bay lowlands.Key words: Arctic, DELTA, floristics, identification, seed morphology.


Author(s):  
A.F. Bukharov ◽  
D.N. Baleev ◽  
E.V. Kashnova ◽  
G.V. Kasaeva ◽  
M.I. Ivanova ◽  
...  

Цель исследований: изучение влияния сортового и экологического факторов на изменчивость морфометрических (длина семени, эндосперма и зародыша) параметров семян моркови в системе двухфакторного опыта. Объекты для изучения – оригинальные семена сортов Шантенэ 2461 и Боярыня. Опыты были заложены в 2015–2017 годах на Западно-Сибирской овощной опытной станции ФГБНУ ВНИИО в южной части Первомайского района Алтайского края. Преобладающие почвы – черноземы выщелоченные и обыкновенные. Повторность опыта трехкратная, в каждой повторности не менее 20 семян. Измерение длины семени и эндосперма проводили штангенциркулем (ГОСТ 166-89), длину зародыша определяли с использованием микроскопа Микромед и видеоокуляра DCM 300 MD. Семена замачивали в 14%-ном водном растворе гипохлорита натрия в течение 1 ч, после чего измеряли длину семени, эндосперма и зародыша и рассчитывали соотношение длины зародыша (E) к длине эндосперма (S). Семена двух сортов моркови, за годы исследования существенно различались по длине семян (Р < 0,001). Длина эндосперма сорта Боярыня в среднем за годы исследований была меньше (Р < 0,001) по сравнению с Шантенэ 2461. Длина зародыша также существенно отличалась (Р < 0,001) в зависимости от сорта. Экологический фактор значительно влиял на длину семени (Р=0,005) и эндосперма (Р=0,003). На длину зародыша основное влияние оказывал фактор сорта (Р < 0,001). На отношение длины зародыша к длине эндосперма также в значительной степени влиял фактор сорта (Р = 0,006). Исследования показали, что изменчивость морфометрических параметров семян обусловлена как влиянием сорта, так и влиянием условий года репродукции. Длина зародыша по сравнению с другими элементами семени варьирует сильнее и в большей степени зависит от влияния наследственного фактора. Это позволяет сделать вывод о возможности селекционного изменения линейных, а также относительных размеров морфологических элементов семени.The article is devoted to the study of the effect of varietal and environmental factors on the variability of morphometric (seed length, endosperm and germ) parameters of carrot seeds. The objects for the study were the original seeds of different varieties grown in 2015, 2016 and 2017. Seeds obtained from two varieties of carrots over the years of research differed significantly in the length of the seeds (P <0.001). The length of the endosperm varieties Boyarynya on average over the years of research was less (P <0.001) compared with Shantane 2461. The experiments were conducted in 2015-2017 at the West Siberian Vegetable Experimental Station in the southern part of the Pervomaisky district of the Altai region. The prevailing soils are leached and ordinary chernozems. Experiment replication is 3, each repeatability consists at least 20 seeds. The measurement of the length of the seed and the endosperm was carried out with Vernier caliper (GOST 166-89), the length of the embryo was determined using microscope Mikromed and video-ocular DCM 300 MD. Seeds were soaked in 14% aqueous sodium hypochlorite solution for 1 h, and then measured the length of the seed, the endosperm and the embryo, and calculated the ratio of the length of the embryo (E) to the length of the endosperm (S). Embryo length was also significantly different (P <0.001) depending on the variety. The environmental factor had a significant impact on the length of the seed (P = 0.005) and endosperm (P = 0.003). The length of the embryo was mainly affected by the factor of the variety (P <0.001). The ratio of the embryo length to the endosperm length was largely influenced by the factor of the variety (P = 0.006). Our studies have shown that the variability of the morphometric parameters of seeds is due to both the influence of the variety and the conditions of the year of reproduction. In comparison with other elements of the seed, the embryo length varies more and depends more on the influence of the hereditary factor. This allows us to conclude about the possibility of a selection change in linear as well as relative sizes of the morphological elements of the seed.


2007 ◽  
Vol 47 (6) ◽  
pp. 683 ◽  
Author(s):  
Pippa J. Michael ◽  
Kathryn J. Steadman ◽  
Julie A. Plummer

Seed development was examined in Malva parviflora. The first flower opened 51 days after germination; flowers were tagged on the day that they opened and monitored for 33 days. Seeds were collected at 12 stages during this period and used to determine moisture content, germination of fresh seeds and desiccation tolerance (seeds dried to 10% moisture content followed by germination testing). Seed moisture content decreased as seeds developed, whereas fresh (max. 296 mg) and dry weight (max. 212 mg) increased to peak at 12–15 and ~21 days after flowering (DAF), respectively. Therefore, physiological maturity occurred at 21 DAF, when seed moisture content was 16–21%. Seeds were capable of germinating early in development, reaching a maximum of 63% at 9 DAF, but germination declined as development continued, presumably due to the imposition of physiological dormancy. Physical dormancy developed at or after physiological maturity, once seed moisture content declined below 20%. Seeds were able to tolerate desiccation from 18 DAF; desiccation hastened development of physical dormancy and improved germination. These results provide important information regarding M. parviflora seed development, which will ultimately improve weed control techniques aimed at preventing seed set and further additions to the seed bank.


2020 ◽  
Vol 42 ◽  
pp. e49895
Author(s):  
Ana Paula de Oliveira Silva ◽  
Isabella Sousa Ribeiro ◽  
Tathiana Elisa Masetto ◽  
Luiz Carlos Ferreira Souza

Determination of seed-maturation indicators enables the identification of the ideal moment for harvest to achieve the best production and conservation potential. Our objective here was to evaluate some physical and physiological changes of crambe (Crambe abyssinica Hochst) seeds as possible indicators of seed maturation. Crambe flowering was monitored in Dourados, Mato Grosso do Sul, Brazil. Plants were tagged, and 13, 22, 26, and 28 days after the initiation of flowering, the seeds were collected and following physical attributes evaluated: length, diameter, total mass, dry matter and water content. Physiological quality of the seeds was assessed using the germination test, by registering the percentage of normal seedlings and dormant seeds, immediately after each harvest, and again after six months of storage. The water-absorption curves were characterized as a function the seed-development stages. All physical attributes were observed to increase because of the accumulation of reserve substances during seed development, except for water content, which gradually decreased from 72.2% at the start of development to 29.5% at maturity. At 28 days after anthesis the germination percentage of crambe seeds at physiological maturity was only 17%, indicating that they became dormant while maturing. However, seed germination rate was 89% after six months of storage, indicating that dormancy was almost fully overcome after this period.


Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 400 ◽  
Author(s):  
Intikhab Alam ◽  
Dong-Li Cui ◽  
Khadija Batool ◽  
Yan-Qing Yang ◽  
Yun-Hai Lu

The HECT-domain protein family is one of the most important classes of E3 ligases. While the roles of this family in human diseases have been intensively studied, the information for plant HECTs is limited. In the present study, we performed the identification of HECT genes in Brassica rapa and Brassica oleracea, followed by analysis of phylogeny, gene structure, additional domains, putative cis-regulatory elements, chromosomal location, synteny, and expression. Ten and 13 HECT genes were respectively identified in B. rapa and B. oleracea and then resolved into seven groups along with their Arabidopsis orthologs by phylogenetic analysis. This classification is well supported by analyses of gene structure, motif composition within the HECT domain and additional protein domains. Ka/Ks ratio analysis showed that these HECT genes primarily underwent purifying selection with varied selection pressures resulting in different rates of evolution. RNA-Seq data analysis showed that the overwhelming majority of them were constitutively expressed in all tested tissues. qRT-PCR based expression analysis of the 10 B. rapa HECT genes under salt and drought stress conditions showed that all of them were responsive to the two stress treatments, which was consistent with their promoter sequence analysis revealing the presence of an important number of phytohormone-responsive and stress-related cis-regulatory elements. Our study provides useful information and lays the foundation for further functional determination of each HECT gene across the Brassica species.


1992 ◽  
Vol 2 (2) ◽  
pp. 89-95 ◽  
Author(s):  
D. Gray ◽  
J. R. A. Steckel ◽  
L. J. Hands

AbstractThe effects of development of leek seeds at 20/10°, 25/15° and 30/20°C (day/night) and drying of seed harvested at different developmental stages on subsequent performance were examined in each of 3 years. An increase in temperature from 20/10° to 30/20°C reduced mean seed weight from 2.90 to 2.55 mg as a result of a reduction in the duration of seed growth from 80 to 55 days; seed growth rate was unaffected. Seed moisture content reached a minimum, up to 35 days after the attainment of maximum seed dry weight and 115, 90 and 70 days after anthesis at 20/10°, 25/15° and 30/20°C, respectively. The curves relating seed moisture to time for each temperature regime were mapped onto a single line accounting for >90% of the variation in moisture content, using accumulated day-degrees >6°C instead of chronological time. Seeds were capable of germinating when seed moisture contents were >60% (fresh weight basis), but maximum viability and minimum mean time to germination were not attained until seed moisture contents at harvest had fallen to 20–30%. Germination was little affected by temperature of seed development. Drying immature seeds increased percentage germination. Growing seeds at 30/20°C and drying at 35°C and 30% RH raised the upper temperature limit of germination compared with growing at 20/10°C and drying at 15°C and 30% RH.


2019 ◽  
Vol 138 (6) ◽  
pp. 733-740
Author(s):  
Huihui Gao ◽  
Yue Gu ◽  
Hongwei Jiang ◽  
Yingying Li ◽  
Dawei Xin ◽  
...  
Keyword(s):  

2011 ◽  
Vol 23 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Roman Hołubowicz ◽  
Maria Morozowska

Effect of umbel position on dill (Anethum graveolensL.) plants growing in field stands on selected seed stalk featuresResearch was carried out on the effect of the umbel position on the ‘Amat’ dill (Anethum graveolensL.) plant on selected features of the seed stalk in the years 2002-2004. Commercial seeds of the species were sown on a small production field and grown for the purpose of producing seeds. Out of the field plant stands, three hundred plants were randomly selected. The following morphological features were then measured in respect to the seed stalk architecture in these plants: the length of the seed stalk, number of branches with primary, secondary and tertiary umbels, length of branches with primary umbels, as well as the main and primary umbel diameters. In addition, seed yield per plant, the weight of 1000 seeds, germination capacity, seed length and width, seed embryo and endosperm lengths for seeds from the main and primary umbels were also calculated and measured. Additionally, the main and primary umbels were divided into three parts: external, middle and internal, and seeds from each were examined separately. The position of the umbel on the seed stalk did not affect the number of branches with primary, secondary, and tertiary umbels on the dill plant and had no effect on its seed stalk architecture. There was no significant difference within the position of the main and primary umbels on the seed stalk grown in field stands. The diameter of the main umbel was the same as the primary one. The lower its position on the seed stalk, the lower the seed yield and germination capacity. The best seeds, in terms of their 1000 seed weight, came from the external part of the main umbel. The different localisation of seeds within the three designated parts of the main umbel had no effect on seed length or width or on their endosperm and embryo lengths. The recorded seed embryo length from the internal part of the primary umbel was smaller than its values from the two other parts of that umbel.


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