scholarly journals Effect of dietarysupplementation with treated amaranth seeds on fermentation parameters in an artificial rumen

2013 ◽  
Vol 58 (No. 4) ◽  
pp. 159-166 ◽  
Author(s):  
P. Kubelková ◽  
D. Jalč ◽  
P. Homolka ◽  
B. Čermák
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Ruminal Fermentation ◽  
Total Volatile ◽  
Microbial Nitrogen ◽  
Acid Detergent Fibre ◽  
Fermentation Parameters

The rumen simulating technique (RUSITEC) was used to evaluate the effect of dietary inclusion of amaranth seeds on the rumen fermentation and the concentration of fatty acids (FA) in fermentation fluid. Four fermentation vessels of the RUSITEC were used. The control diet (C) consisted of 70% meadow hay and 30% barley meal, whereas 10% of barley meal was replaced by milled (A<sub>mill</sub>), mechanically ground (A<sub>gr</sub>), and ground after heating in a microwave amaranth seeds (A<sub>heat+gr</sub>) in other experimental vessels, respectively. All diets were isonitrogenous (11% of crude protein). With degradability of dry matter (DM) and acid-detergent fibre (ADF) not affected (P &gt; 0.05), degradability of neutral detergent fibre (NDF) was significantly decreased by the inclusion of A<sub>mill</sub> (P &lt; 0.001) and A<sub>heat+gr</sub> (P &lt; 0.05). Heating, compared to milling, decreased degradation of crude protein (CP) and addition of amaranth seeds generally increased production of microbial nitrogen. Production of fermentation gasses, methane, and total volatile fatty acids (VFA) was not affected by changes in composition of diets. Addition of amaranth seeds in a milled form (A<sub>mill</sub>) caused a significant decrease in concentrations of acetate and propionate and growth in concentrations of butyrate, iso-valerate, and caproate. No effect (P &gt; 0.05), compared to C, was found on the percentage of saturated or unsaturated FA, but changes in concentrations of some FA were observed. An absence of any detrimental effects on ruminal fermentation patterns indicated that grain amaranth seeds can partially substitute for the barley in ruminant nutrition.

The roughage requirement of the early-weaned calf

1973 ◽  
Vol 16 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Hi Shin Kang ◽  
Jane Leibholz
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Wheat Straw ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Linear Increase ◽  
Total Volatile ◽  
Dry Matter Digestibility ◽  
Acid Detergent Fibre ◽  
Ad Libitum

SUMMARYAn experiment was conducted with 50 male Friesian calves between 5 and 11 weeks of age. Milled wheat straw (0·95 cm screen) was included in a pelleted all-concentrate diet at concentrations of 0, 15, 30 or 45 %. The nitrogen content of the diets was maintained by the addition of urea. The diets were given alone or with a chaffed lucerne hay or wheat straw supplement ad libitum. All diets contained 2 % sodium bicarbonate.The feed intake and weight gains of the calves were increased significantly by the inclusion of 15 % milled wheat straw, but were reduced by 30 or 45 % straw. The supplements of chaffed wheat straw or lucerne hay ad libitum did not influence the performance of the calves. The inclusion of wheat straw in the diets reduced their dry-matter digestibility but the digestibility of acid detergent fibre was maximal with 27 % straw. The digestibility of nitrogen was significantly increased by 15% straw but reduced by further increases in straw.The inclusion of wheat straw in the diets did not influence the pH of the rumen contents or the concentration of total volatile fatty acids in the rumen. Increasing the level of straw in the diet caused a significant linear increase in the proportion of acetic acid and decreases in butyric, propionic and valeric acids.

Effects of feeding wheat middlings on production, digestibility, ruminal fermentation and carcass characteristics in beef cattle

2003 ◽  
Vol 83 (3) ◽  
pp. 551-557 ◽  
Author(s):  
D. R. ZoBell ◽  
L. A. Goonewardene ◽  
K. C. Olson ◽  
C. A. Stonecipher ◽  
R. D. Wiedmeier
Keyword(s):  
Fatty Acids ◽  
Beef Cattle ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Ruminal Fermentation ◽  
Total Volatile ◽  
Wheat Middlings ◽  
Daily Gain

Studies were conducted to compare the feed value of wheat middlings (WM) to cereal grains in rations with varying concentrate to roughage levels. In two separate studies, weaned heifers and steers were fed corn silage/alfalf a hay-based growing diets where the concentrate source was either a control diet consisting of rolled barley (heifers) or rolled corn (steers) versus WM, over an 84-d period. In a 107-d study, finishing steers were fed treatments that consisted of rolled corn (C) plus either 35% wheat middlings (WM35) or 50% wheat middlings (WM50). A single cross-over designed digestibility study was also conducted utilizing four cannulated yearling heifers fed either a corn or WM50 diet from the finishing steer study. Results from the heifer and growing and finishing steer studies showed that average daily gain (ADG), dry matter intake (DMI) and feed efficience (FE) were not affected (P > 0.05) by feeding treatment. Carcass characteristics, including hot carcass weight, ribeye area, backfat, yield, quality grade and cutability were also unaffected (P > 0.05) by feed treatment. Results from the digestibility studies indicated total volatile fatty acids (VFA) were increased (P = 0.023) in the WM diet, with acetate lower (P = 0.0003) than the C group, and pH levels of 5.81 and 5.55 for the C and WM treatments respectively (P = 0.011). Dry matter and ADF digestibilities were not affected (P > 0.05) by feed treatment. It is concluded from these studies that WM can be fed to growing beef heifers and steers as an alternative to more traditional concentrate sources such as corn or barley. Wheat middlings could replace up to 50% of the concentrate in a finishing ration. However, certain ruminal fermentation characteristics such as pH, acetate: propionate ratio and total volatile fatty acids may be altered, which could affect performance over an extended feeding period. Key words: Wheat middlings, average daily gain, feed efficiency, digestibility, ruminal degradation, beef cattle

scholarly journals Relations of Ruminal Fermentation Parameters and Microbial Matters to Odd- and Branched-Chain Fatty Acids in Rumen Fluid of Dairy Cows at Different Milk Stages

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1019 ◽  
Author(s):  
Keyuan Liu ◽  
Yang Li ◽  
Guobin Luo ◽  
Hangshu Xin ◽  
Yonggen Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Nitrogen Flow ◽  
Ruminal Fermentation ◽  
Bacterial Populations ◽  
Microbial Nitrogen ◽  
Branched Chain Fatty Acids ◽  
Branched Chain ◽  
Fermentation Parameters ◽  
Chain Fatty Acids

The purpose of this research was to evaluate whether relationships exist between odd- and branched-chain fatty acids (OBCFAs) originating from milk fat and the corresponding data of ruminal fermentation parameters, microbial populations, and base contents that were used to mark microbial protein in rumen. Nine lactating Holstein dairy cows with similar body weights and parity were selected in this study, and the samples of rumen and milk were collected at the early, middle, and late stages, respectively. The rumen and milk samples were collected over three consecutive days from each cow, and the ruminal and milk OBCFA profiles, ruminal fermentation parameters, bacterial populations, and base contents were measured. The results showed that the concentrations of OBCFAs, with the exception of C11:0 and C15:0, were significantly different between milk and rumen (p < 0.05). The concentrations of anteiso-fatty acids in milk were higher than those in rumen, and the contents of linear odd-chain fatty acids were higher than those of branched-chain fatty acids in both milk and rumen. Significant relationships that existed between the concentrations of C11:0, iso-C15:0, anteiso-C15:0, C15:0, and anteiso-C17:0 in rumen and milk (p < 0.05). The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid contents (p < 0.05). The populations of Ruminococcus albus, R. flavefacients, and Eubacterium ruminantium were significantly related to milk C13:0 contents (p < 0.05). The adenine/N ratio was negatively related to milk OBCFA content (p < 0.05) but positively associated with the iso-C15:0/iso-C17:0 ratio (p < 0.05). Milk OBCFAs were significantly correlated with ruminal fermentation parameters, ruminal bacterial populations, and base contents. Milk OBCFAs had the potential to predict microbial nitrogen flow, and the prediction equations for ruminal microbial nitrogen flow were established for OBCFAs in dairy milk.

ALFALFA SUPPLEMENTATION OF CORNCOB DIETS FOR SHEEP: COMPARISON WITH HIGHER VOLATILE FATTY ACIDS, SOYBEAN PROTEIN AND ALFALFA FIBER ON INTAKE, ENVIRONMENT AND DIGESTION IN THE RUMEN AND DIGESTA PASSAGE

1987 ◽  
Vol 67 (4) ◽  
pp. 1083-1091 ◽  
Author(s):  
L. R. NDLOVU ◽  
J. G. BUCHANAN-SMITH
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Soybean Protein ◽  
Control Diet ◽  
Basal Diet ◽  
Latin Squares ◽  
Protein Preparation ◽  
Alfalfa Hay

Nine lambs fitted with rumen cannulae were used in two 5 × 5 Latin squares to measure the effect of alfalfa hay, C4- and C5-branched- and straight-chain volatile fatty acids (C4/C5 VFAs), cell wall from alfalfa hay (ALFCW) and a soybean protein preparation on intake and rumen digestion of a basal corncob diet with urea included at 30 g kg−1. Relative to the control, alfalfa hay decreased (P < 0.05) intake of the basal diet but increased (P < 0.05) total dry matter intake. ALFCW and soybean protein did not affect intake of the basal diet and total dry matter (P > 0.05). C4/C5 VFAs increased intake of basal diet by sheep in one square (P < 0.05) but had no effect (P > 0.05) on intake with sheep in the other square. Alfalfa hay, ALFCW and soybean protein increased (P < 0.05) total VFA concentration in rumen fluid while only alfalfa hay and C4/C5 VFAs consistently increased isobutyrate, isovalerate and valerate levels in rumen fluid (P < 0.05). In comparison with the control diet, soybean protein elevated (P < 0.05) mean rumen ammonia-N levels whereas C4/C5 VFAs depressed them; however, values exceeded 17 mg dL−1 rumen fluid on all treatments. Most measures of digestion kinetics of fiber from corncobs were unaffected by supplementation (P > 0.05). Alfalfa hay and ALFCW increased (P < 0.05) rate of passage of digesta from the reticulo-rumen. It was concluded that alfalfa hay has a reduced role in improving intake of corncob diets when there is also sufficient NPN in the diet. Key words: Roughage (poor quality), volatile fatty acids, rumen ammonia levels, chromium-mordanted cell walls

Effects of urea and saccharose on intake and digestion of a Digitaria decumbens hay by black belly sheep

1999 ◽  
Vol 69 (2) ◽  
pp. 403-410 ◽  
Author(s):  
H. Archimède ◽  
G. Aumont ◽  
G. Saminadin| ◽  
E. Deprès ◽  
P. Despois ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nitrogen Availability ◽  
Latin Square ◽  
Live Weight ◽  
Ruminal Bacteria ◽  
Digitaria Decumbens ◽  
Acid Detergent Fibre ◽  
Kinetics Of

AbstractEffects of incorporation of urea and saccharose in diets, on intake and digestion by sheep of a 35-day-old pangola (Digitaria decumbens) hay, have been studied according to a 4 × 4 Latin-square design. Sixteen rams were given four diets: hay (C); hay plus urea (U, 23 g/kg hay); hay plus saccharose (S, 60 g/kg hay), hay plus urea and saccharose (SU). Acid-detergent fibre and crude protein (CP) content of the roughage were 395 and 78 g/kg dry matter (DM) respectively. Intake of hay (g DM per kg live weight0.75), supplemented with urea and sugar (42⋅9), was lower (F < 0.05) than with other diets (47.2, s.e. 1.6). The organic matter (OM) total tract and ruminal digestibility of the diet C, S, U and SU were 0.622, 0.590, 0.615 and 0.587 (s.e. 0.007); 0.361, 0.380, 0.378 and 0.345 (s.e. 0.015) respectively. Effective degradation and nylon bag kinetics of DM degradation were higher with U and SU compared with S or C. Few differences were observed between diets for ruminal concentration of volatile fatty acids. The ruminal ammonia concentrations were higher (P < 0.05) for the diets with urea than without urea (78 v. 215 mg/l). The efficiencies of the microbial synthesis (g nitrogen per kg OM fermented in the rumen) were, 23.6, 22.4, 24.9 and 29.3 (s.e. 1.7) for the diets C, U, S and SU respectively.Even though additional urea increased nitrogen availability for ruminal bacteria, urea supplementation did not affect significantly intake or digestion of the pangola hay of medium CP content used in this experiment.

Optimalisasi Penggunaan Level Sabun Kalsium Minyak Kedelai dalam Ransum Terhadap Karakteristik Fermentasi, Populasi Mikroba dan Kecernaan Nutrien Secara In Vitro Menggunakan Cairan Rumen Sapi Bali

2018 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Ali Bain ◽  
Komang G. Wiryawan ◽  
Dewi Apri Astuti ◽  
Chairrusyuhur Arman ◽  
Sri Suharti
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Microbial Population ◽  
Total Volatile ◽  
Calcium Soap ◽  
Total Bacteria ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi optimalisasi penggunaan level sabun kalsium yang berbeda dalam ransum secara in vitro terhadap karakteristik fermentasi, populasi mikroba dan kecernaan nutrien ransum, menggunakan sumber inokulum cairan rumen sapi Bali. Penelitian dilaksanakan menggunakan rancangan acak kelompok dengan 4 jenis perlakuan dalam 3 ulangan.  Ransum penelitian terdiri atas : R1, 40% rumput lapang (RL) + 60% konsentrat (K), R2 (40% RL + 60% K, mengandungn 2.5% SCa-kedelai), R3 (40%  RL + 60% K, mengandung 5% SCa-kedelai), R4 (40%  RL + 60% K, mengandung 7.5% SCa-kedelai). Variabel yang diukur terdiri atas karakteristik fermentasi in vitro (pH, N-NH3, produksi total volatile fatty acids), populasi mikroorganisme (total bakteri dan total protozoa), dan kecernaan nutrien (kecernaan bahan kering dan kecernaan bahan organik). Data dianalisis menggunakan analysis of varians  (ANOVA) dan perbedaan antara perlakuan diuji dengan Duncan’s Multiple Range Test.  Hasil penelitian menunjukkan, penambahan SCa-kedelai pada level yang berbeda dalam konsentrat tidak mempengaruhi pH, konsentrasi amonia, populasi mikroba, kecernaan bahan kering dan kecernaan bahan organik fermentasi tetapi berpengaruh sangat nyata (P<0.002) terhadap produksi total VFA. Produksi total VFA tertinggi diperoleh pada ransum perlakuan R4 (konsentrat yang mengandung 7.5% SCa-kedelai). Produksi total VFA tertinggi diperoleh pada perlakuan R4 dan produksi total VFA paling rendah terjadi pada perlakuan ransum R1 (kontrol).  Produksi total VFA  ransum R1 dan R2 dan ransum R2 dan R3 tidak berbeda nyata namun produksi total VFA ransum R3 lebih tinggi dibanding ransum kontrol. Penambahan SCa-kedelai dalam konsentrat menghasilkan produksi total VFA tetinggi namun mulai menekan populasi total bakteri. Mempertimbangkan hasil peubah fermentasi dan populasi mikroba serta biaya pembuatan produk sabun kalsium, SCa-kedelai pada level 5% merupakan level penggunaan SCa-kedelai yang terbaik dalam ransum.Kata kunci: sabun kalsium, fermentasi, in vitro, kecernaanABSTRACTThe study to evaluate the optimalization the different level of soybean oil calcium soap (CaS-soybean) in ration on in vitro fermentation using Bali cattle rumen fluid. The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. Ration treatments were R1: 40% native grass (NG) + 60% concentrate (C), R2 : 40% NG + 60% C, supplemented with 2.5% soybean oil calcium soap (CaS-soybean), and R3 (40% NG + 60% C, supplemented with 5.0% CaS-soybean and R4 (40% NG + 60% C, supplemented with 7.5% CaS-soybean). Variables measured were fermentation characteristics (pH, NH3-N, total volatile fatty acids), microbial population (total bacteria and total protozoa), and nutrient digestibility (dry matter and organic matter digestibility). Data were analyzed using analysis of variance (ANOVA) and the differences between treatments means were examined by Duncan Multiple Range Test. Results of the study  showed that the four different level of CaS-soybean in ration did not have any significant effect (P>0,05) on pH, NH3-N, total bacteria, total protozoa, dry matter and organic matter digestibility. The treatments significantly increased (P<0.05) the production of total volatile fatty acids.  The highest production of total VFA was obtained from ration R4 (concentrate containing 7.5% CaS-soybean) and the lowest was obtained from treatment R1 (control). There were no significant differences between R1 and  R2, and between R2 and R3 on the total VFA production. However, the total productions of VFA in ration R3 were higher than that of the control ration. The addition of CaS-soybean in the concentrate had increased the total production of VFA, but at the same time, it began pressing the total population of bacteria. Considering the results of the fermentation variables and microbial population as well as the cost of making calcium soap products, CaS-soybean at 5% level was selected as the best level of CaS-soybean in ration.Keywords : calcium soap, fermentation, in vitro, digestibility

Effects of dietary calcium soaps of unsaturated fatty acids on digestion, milk composition and physical properties of butter

1997 ◽  
Vol 64 (2) ◽  
pp. 181-195 ◽  
Author(s):  
FRANCIS ENJALBERT ◽  
MARIE CLAUDE NICOT ◽  
CORINE BAYOURTHE ◽  
MICHELE VERNAY ◽  
RAYMOND MONCOULON
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fat ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Control Diet ◽  
Milk Composition ◽  
Ruminal Fermentation ◽  
Maize Silage ◽  
Ruminal Biohydrogenation

Dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with Ca soaps (CaS) of palm fatty acids (FA) and rapeseed FA. Diets compared were control diet based on maize silage and concentrate, and two diets with 40 g CaS of palm oil FA or rapeseed oil FA/kg diet, replacing part of the concentrates of the control diet. Total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving CaS; the extent of ruminal biohydrogenation of total unsaturated C18 FA was significantly reduced by both CaS diets. Apparent intestinal digestibility of FA was not different among diets, although the amount of FA absorbed with the CaS diets was twice that with the control diet. No difference among diets was observed for milk production, or fat and protein contents. Giving CaS diets decreased the proportions of 4[ratio ]0 to 14[ratio ]0 FA in milk fat, and increased cis-18[ratio ]1n−9, compared with control diet. The rapeseed diet lowered the content of 16[ratio ]0, and increased the contents of 18[ratio ]0 and trans-18[ratio ]1n−7. CaS diets did not result in a marked increase of polyunsaturated FA content in milk fat. Butter from cows fed on the CaS diets contained more liquid fat at 6 and 14°C than butter from the cows fed on the control diet. Incorporating CaS, particularly those from rapeseed, in dairy cows' diets increased C18 FA in milk and improved butter spreadability.

ANATOMICAL AND PHYSIOLOGICAL CHANGES IN LAMBS DURING BACTERIAL INHIBITION

1967 ◽  
Vol 45 (3) ◽  
pp. 433-441 ◽  
Author(s):  
M. R. Karr ◽  
C. O. Little ◽  
G. E. Mitchell Jr. ◽  
H. A. Glimp ◽  
P. G. Woolfolk
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Bacterial Population ◽  
Structural Development ◽  
Physiological Changes ◽  
Maintain Body Weight ◽  
Reduced Rate

Rumen cannulas were installed in four sets of twin lambs. Increasing levels (with time) of a mixture of antibiotics and Sulfathalidine were administered via the rumen cannula twice daily to prevent the development of a bacterial population in the rumen. This procedure was successful for 12 weeks. Small concentrations of volatile fatty acids were observed after this time. Neither papillae nor the muscular part of the rumen developed normally in the treated lambs where rumen volatile fatty acids were absent. Structural development of the reticulum and omasum was also greatly reduced by the antibiotic–Sulfathalidine treatments. In vitro butyrate uptake by tissues taken from the rumen, reticulum, and omasum was similar and was low for both the control and treated lambs. Poor muscular development of the rumen wall was observed in the treated lambs; and large quantities of ingesta were present, suggesting a reduced rate of passage. This was associated with diminished feed intake and inability of the lambs to maintain body weight after weaning. The apparent digestion of dietary dry matter, crude protein, starch, and cellulose was reduced by treatment.

The fermentable metabolisable energy in grass silage

1993 ◽  
Vol 1993 ◽  
pp. 141-141
Author(s):  
A T Chamberlaina ◽  
K Seyoum ◽  
D Chapman ◽  
C Piotrowski
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Energy Content ◽  
Ether Extract ◽  
Potential Yield ◽  
Acid Detergent Fibre ◽  
Gross Energy ◽  
Metabolisable Energy ◽  
The Uk

The UK metabolisable protein system (AFRC, 1992) requires the measurement of fermentable metabolisable energy (FME) to determine the potential yield of microbial crude protein in the rumen. FME is defined as:The objective of this work was to measure the ME , ether extract and volatile fatty acid components of first cut grass silages to assess the range of FME and non-fermentable fractions and hence the range of fermentabilities (FME:ME).Eighty-four clamp grass silages, predominantly first-cut rye grass, were analysed for modified acid detergent fibre (MADF), individual volatile fatty acids, oil (ether extract) and oven dry matter. ME was predicted from the MADF after correction for oven dry matter. The gross energy of the lactic, acetic, propanoic, butyric and valeric acid were taken to be 15.2, 14.6, 20.8, 24.9, and 28.0 MJ/kg DM, and for the Ether Extract fraction of grass 38.5 MJ/kg DM. The energy content of the volatile fatty acids in the silage were summed and FME was calculated as described above. The fermentability of the silage was the expressed as the proportion of FME to ME.

scholarly journals THE EFFECT OF CONCENTRATE ON THE DIGESTION OF BROMEGRASS CONSTITUENTS

1981 ◽  
Vol 61 (4) ◽  
pp. 935-944 ◽  
Author(s):  
K. K. FRANKLIN ◽  
J. E. WINCH ◽  
G. K. MACLEOD
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Total Volatile ◽  
Acid Detergent Fiber ◽  
Concentrate Supplementation ◽  
Wall Components

A nylon bag technique was used to study the effect of concentrate feeding on the rate and extent of digestion of bromegrass constituents in eight fistulated sheep fed rations at or slightly above maintenance, at concentrate to bromegrass ratios of 3:97, 22:78, 44:56 and 66:34. The eight sheep were arranged in a double 4 × 4 latin square design with nylon bag incubation times of 3, 6, 12, 24 and 48 h. Rations containing 44 and 66% concentrate depressed rumen digestion of bromegrass dry matter at both 24- and 48-h incubation times. The extent of digestion depression varied among sheep and was related to the animal's ability to buffer increases in rumen acidity. Digestibilities of neutral detergent fiber, acid detergent fiber, cellulose and hemicellulose followed trends similar to that of dry matter. Rumen osmolality, total volatile fatty acids and ammonia levels appeared to have little relationship to bromegrass dry matter digestion. The apparent or total gut digestibility of cell wall components was not depressed. This suggested that additional digestion took place in the ileum, cecum and colon and that the proportion of concentrate supplementation may affect ruminal but not the total gut utilization of roughage at maintenance levels of intake.

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