scholarly journals Apparent ileal amino acids digestibility of diets with graded levels of corn DDGS and determination of DDGS amino acids digestibility by difference and regression methods in broilers

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 164-169 ◽  
Author(s):  
M. Foltyn ◽  
M. Lichovníková ◽  
V. Rada ◽  
A. Musilová
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Regression Methods ◽  
The Difference ◽  
Nonessential Amino Acids ◽  
Different Levels ◽  
Corn Ddgs

This study was conducted to determine apparent ileal amino acids digestibility (AIAAD) of diets with different levels of corn distiller’s dried grains with solubles (DDGS) and to determine AIAAD of corn DDGS by difference and regression methods in broiler chickens. One hundred and fifty 30-day-old male broiler chickens were used in the experiment. The corn DDGS were incorporated into basal diets at graded levels (0, 4, 8, 12, and 16%). All birds were killed at the age of 35 days and the contents of the lower half of the ileum were collected. AIAAD was calculated using chromic oxide as the indigestible marker. For AIAAD of DDGS determination, difference and regression methods were used. AIAAD of Lys (78.6%) and Met (91.3%) were the significantly highest in the diet without DDGS in comparison with other diets (P < 0.01). AIAAD of the rest of essential and nonessential amino acids were the lowest in the diet with 8% of DDGS in comparison with the diets with 0 or 16% of DDGS (P < 0.01). The apparent ileal crude protein digestibility was also the lowest in the diet with 8% (61.8%) of DDGS (P < 0.01). The highest differences in AIAAD of DDGS determined by the difference method and in the diet without DDGS (basal diet) determined by the regression methods were found in Lys and Met. There were very low differences between DDGS and basal diet in AIAAD of nonessential amino acids, except Ala and Asp. These results show that higher levels of DDGS decrease AIAAD.  

scholarly journals Comparison of methodologies to determine the apparent ileal amino acid digestibility of maize, wheat, lupins, and peas for broiler chickens

2021 ◽  
pp. 1-6
Author(s):  
C.L. Nalle ◽  
V. Ravindran
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Difference Method ◽  
Broiler Chickens ◽  
Direct Method ◽  
Basal Diet ◽  
Sole Source ◽  
Grain Legumes ◽  
Main Effect ◽  
The Difference

The influence of method (direct vs difference method) used to determine the apparent ileal digestibility coefficient (AIDC) of amino acids in two cereals (maize and wheat) and two grain legumes (Australian sweet lupins and peas) was investigated. For the direct method, the test ingredients were incorporated as the sole source of protein in assay diets. The assay diets used in the difference method were formulated by substituting the cereals and legumes for 50 and 25% (w/w), respectively, of a maize-soy basal diet. Each diet contained 3 g/kg titanium dioxide as an indigestible marker and were offered ad libitum to four replicate cages of broilers (four birds/cage) from d 28 to d 35 post-hatching. On d 35, digesta were collected from the terminal ileum and the AIDC of amino acids were calculated using marker ratios in the diet and digesta. Except for histidine, methionine, cysteine and tyrosine, the main effect of methods was found to be significant (P<0.05 to 0.001) for the AIDC of all amino acids. The main effect of the ingredients was significant (P<0.05 to 0.001) for all amino acids, except for histidine, aspartic acid and serine. Interactions (P<0.05) between ingredients and method were observed only for leucine, lysine, proline and serine. The AIDC of amino acids, determined by the difference method, was found to be distinctly higher than those determined by the direct method. This suggested that the direct method underestimated amino acid digestibility in low and medium protein ingredients.

scholarly journals PSVII-21 Determination of apparent and standardized ileal digestibility of amino acids in various hatchery by-products for broiler chickens

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 354-354
Author(s):  
Geun Hyeon Park ◽  
Hyeon Seok Choi ◽  
Jong Hyuk Kim ◽  
Gi Ppeum Han ◽  
Sang Yun Ji ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Sole Source ◽  
Low Grade ◽  
Limited Information ◽  
Nutritional Values ◽  
Ileal Digestibility ◽  
Dietary Treatments ◽  
By Products

Abstract The Korean poultry industry produces more than 4,000 tons of hatchery by-products (HBPs) every year. There is an increasing interest in the use of HBPs as a feed ingredient in The Korean feed industry. However, limited information regarding nutritional values for HBPs has been available. Thus, we conducted an experiment to measure apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AAs) in 4 different HBPs for broiler chickens. Four HBPs included infertile eggs (IFE), unhatched eggs (UHE), low grade and dead chicks (LDC), and mixture (MIX) containing 55% IFE, 10% UHE, 10% LDC, and 25% hatched eggshells. A total of three-hundred-thirty-six 21-d-old Ross 308 broiler chickens were randomly allotted to 1 of 4 dietary treatments with 7 replicates per treatment. Each diet contained individual HBP as a sole source of AAs. Additional 125 birds were used to estimate ileal endogenous losses of AAs. Results indicated that the AID and SID of 5 essential AAs (Arg, Met, Trp, His, and Val) for LDC were greatest (P &lt; 0.05), but those (Arg, Met, and Trp) for MIX were the least (P &lt; 0.05) among 4 HBPs. The AID and SID of most AAs in IFE were comparable to those in UHE. However, the AID and SID of 3 essential AAs (Lys, Leu, and Phe) were not different among 4 HBPs. The average AID and SID of both essential and nonessential AAs (18 AAs) for LDC were greatest (P &lt; 0.05), but those for MIX were the least (P &lt; 0.05) among 4 HBPs. In conclusion, HBPs used in this experiment contain high amounts of digestible AAs, which are comparable to other animal by-products. Generally, AAs in LDC are more digestible than AAs in other 3 HBPs (UHE, IFE, and MIX).

scholarly journals Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Nutritive Value ◽  
Animal Feed ◽  
Basal Diet ◽  
Rapeseed Meal ◽  
Metabolizable Energy ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Apparent Metabolizable Energy

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.

scholarly journals Determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases

2009 ◽  
Vol 390 (3) ◽  
Author(s):  
Takayuki K. Nemoto ◽  
Toshio Ono ◽  
Yu Shimoyama ◽  
Shigenobu Kimura ◽  
Yuko Ohara-Nemoto
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protease Activity ◽  
Binding Pocket ◽  
Catalytic Triad ◽  
Amino Acid Residues ◽  
Proteolytic Activities ◽  
Hydrophobic Amino Acids ◽  
The Difference

Abstract Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus warneri secrete glutamyl endopeptidases, designated GluV8, GluSE, and GluSW, respectively. The order of their protease activities is GluSE<GluSW<<GluV8. In the present study, we investigated the mechanism that causes these differences. Expression of chimeric proteins between GluV8 and GluSE revealed that the difference is primarily attributed to amino acid residues 170–195, which define the intrinsic protease activity, and additionally to residues 119–169, which affect the proteolytic sensitivity. Among nine substitutions present in residues 170–195 of the three proteases, the substitutions at positions 185, 188, and 189 were responsible for the changes in their activities, and the combination of W185, V188, and P189, which naturally occurs in GluV8, exerts the highest protease activity. W185 and P189 were indispensable for full activity, but V188 could be replaced by hydrophobic amino acids. These three amino acid residues appear to create a substrate-binding pocket together with the catalytic triad and the N-terminal V1, and therefore define the K m values of the proteases. We also describe a method to produce a chimeric form of GluSE and GluV8 that is resistant to proteolysis, and therefore possesses 4-fold higher activity than the wild-type recombinant GluV8.

scholarly journals Effect of Adding Different Levels of Portulaca oleracea L. Seeds and Leaves Powder to The Diet on Productive and Physiological Performance of Broiler Chickens (Ross 308)

2021 ◽  
Vol 34 (1) ◽  
pp. 38-48
Author(s):  
Ahmed J. Shirshaab ◽  
Jaffar M. Jassim
Keyword(s):  
Broiler Chickens ◽  
Basal Diet ◽  
Serum Glucose ◽  
Portulaca Oleracea ◽  
Control Group ◽  
Broiler Chicks ◽  
Positive Role ◽  
Randomized Design ◽  
Escherichia Coli Bacteria ◽  
Different Levels

This study was conducted in the Poultry Field of the College of Agriculture, Basrah University for the period from 20/9/2019 to 24/10/2019 to investigate the effect of adding different levels of Portulaca oleracea L. seeds and leaves powder on some physiological characteristics of broilers. A total 216 one day old unsexed Ross-308 broiler chicks were used in this study. The chicks were randomly distributed into six treatments replicated three times (12 chicks each replicate). The experiment was designed as a completely randomized design (CRD). The first treatment was control (basal diet), P. oleracea seed powder was added as 5 & 10 g.kg-1 for the 2nd and 3rd treatments. Leaves powder was added as 5 and 10 g.kg-1 to the basal diet in treatment 4th and 5th. In the 6th treatment 5 gm of seed and 5 gm of leaves powder was added to the basal diet. The results showed that there was a significant (P <0.05) decrease in serum glucose, cholesterol and triglyceride concentrations in all additive treatments compared to control. There was also a significant decrease (P <0.05) in Escherichia coli bacteria count, as well as a significant improvement (P <0.05) in the number of Lactobacilli bacteria in all addition treatments compared to the control group. The addition of P. oleracea seeds and leaves powder had no significant effect on some haematological indices and on the total protein concentration in the serum. The study confirms the importance of adding powder of seeds or leaves in the broiler diet because it has a positive role in improving some of the physiological and microbial blood characteristics of the broiler.

Quality of gluten-free cookies made with rice flour of different levels of amylose and cowpea beans

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Estefania Julia Dierings de Souza ◽  
Aline Machado Pereira ◽  
Mauro Fontana ◽  
Nathan Levien Vanier ◽  
Marcia Arocha Gularte
Keyword(s):  
Amino Acids ◽  
Protein Digestibility ◽  
Rice Flour ◽  
Essential Amino Acids ◽  
Gluten Free ◽  
Content Type ◽  
High Amylose ◽  
Different Levels

PurposeThe objective of this study was to evaluate the effects of rice flour obtained from rice grains with different levels of amylose on technological, nutritional and sensory properties of cookies made with a blend of rice and cowpea flour.Design/methodology/approachThe cookies preparation was set at a ratio of rice flour and cowpea beans 70:30. The studied formulations were: LA: low amylose rice flour; MA: medium-amylose rice flour; HA: high amylose rice flour. The quality of the obtained cookies was analyzed for proximate composition, in vitro protein digestibility, thickness, diameter, dispersion factor, texture, color, amino acid profile and sensory properties.FindingsProximate composition and in vitro protein digestibility showed no differences between the three studied formulations. The medium and low amylose rice flour cookies showed the lowest hardness values. The combination of rice and beans allowed a good balance of essential amino acids. The cookies formulated with high amylose rice flour presented lighter coloration, low hardness and greater sensory preference.Originality/valueRice and cowpea flours are an alternative source for the preparation of gluten-free bakery products, such as cookies. The high amylose content of rice flour has less negative interference in the texture characteristics of the cookies. The combination of rice and beans flour provides a balance of essential amino acids.

Methionine, cystine and choline requirements of the growing pig

1984 ◽  
Vol 1984 ◽  
pp. 58-58
Author(s):  
Lynda J. Ferrier ◽  
D.J.A. Cole ◽  
D. Lewis
Keyword(s):  
Amino Acids ◽  
Choline Chloride ◽  
Basal Diet ◽  
Blood Parameters ◽  
Initial Experiment ◽  
Methyl Donors ◽  
Growing Pig ◽  
Inclusion Level ◽  
Sulphur Amino Acids ◽  
Different Levels

In addition to acting as a source of the amino acid itself, methionine can act as a source of methyl groups. The requirement for sulphur amino acids is therefore influenced by the inclusion level of alternative methyl donors in the diet e.g. choline. An initial experiment was designed to ensure an adequate supply of choline so that in subsequent studies the need for methionine per se could be established. Blood metabolites were used to study the requirements. Three diets with different levels of methionine plus cystine (3.5, 4.5 and 5.5 g/kg) were formulated and randomly assigned to 36 pigs. To a basal diet, containing 660 mg/kg choline, choline chloride was added producing diets with choline levels of 660, 860, 1060, 1260, 1460 and 1660 mg/kg. The pigs were randomly allocated three levels of choline, each for a period of three days; this ensured that each treatment was repeated six times. Blood samples were taken by jugular venepuncture 4 hours after feeding at the end of each 3 day period and repeated at 3 liveweights (35, 55 and 75 kg). Blood parameters (urea and plasma amino acids) were used to measure the response.

scholarly journals Influence of Different Time and Frequency of Multienzyme Application on the Efficiency of Broiler Chicken Rearing and Some Selected Metabolic Indicators

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 450
Author(s):  
Youssef A. Attia ◽  
Mohammed A. Al-Harthi ◽  
Ali S. El-Shafey
Keyword(s):  
High Density Lipoprotein ◽  
Broiler Chickens ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Protein Digestibility ◽  
High Density ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Continuous Application ◽  
The Cost

This study looks at the influence of time and/or frequency of multienzymes application on productivity, carcass characteristics, metabolic profile, and red blood cell characteristics of broiler chickens. Two hundred and eighty, one-day-old Arbor Acres broiler male chicks were randomly distributed into seven treatment groups. Each group consisted of eight replicates of five unsexed birds. The same basal diet was fed in a crumble form to all experimental groups: group one was the unsupplemented control that did not receive multienzymes supplementation. Additionally, multienzymes in water were supplemented in six groups in a factorial arrangement, including three times of application (starter time only which included days 1–21 of age, grower time only which included days 22–37 of age, and starter and grower time which included days 1–37 of age) and two application frequencies (continuously or intermittently). In the continuous application, the multienzymes were added to water over 24 h in a day, while in the intermittent frequency multienzymes were added to water for one day followed by a day off according to the time of application. Regardless of time and frequency of application, enzymes supplementation significantly increased growth rate, feed intake, European Production Index (EPI), protein digestibility, serum albumin, and high-density lipoprotein (HDL). Intermittent multienzymes application during days 1–21 of age or days 22–37 of age resulted in significantly greater growth, better feed conversion rate (FCR), and higher EPI of broilers during the whole rearing period than those under continuous multienzymes during different growth periods. Besides, intermittent multienzymes addition during days 1–37 of age improved FCR of broiler chicks compared to constant application. The intermittent addition of multienzymes during days 1–21 of age or 22–37 days of age and days 1–37 of age caused a significant increase in dry matter (DM) digestibility than the continuous application. The intermittent addition of multienzymes during days 1–21 of age significantly increased the digestibility of crude protein (CP), ether extract (EE), and crude fiber (CF) compared to continuous application. A similar trend was shown in the digestibility of CP and EE due to intermittent use during days 22–37 of age. Intermittent enzymes addition significantly increased high density lipoprotein (HDL) of groups receiving enzymes during days 22–37 of age compared to continuous application of enzymes. In conclusion, the use of multienzymes intermittently during days 1–21 of age and 22–37 days of age significantly increased growth, improved FCR, and raised EPI. Intermittent use can replace continuous multienzyme applications which can save 68.6% of the cost, even though further research is need from the cost-saving edge.

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