Evaluation of the Chemical Profiling, Total Phenolic Composition, the Antioxidant and Antimicrobial Properties of the Essential Oils of Mentha piperita L., Salvia officinalis L., and Thymus vulgaris L.

Sage (Salvia officinalis) is the best-known species of the genus Salvia, due to its medicinal and flavouring properties. This research was conducted on samples of S. officinalis collected from Potoci (SOP) in the continental part of Montenegro and from Valdanos (SOV) and Lustica (SOL) in the country?s coastal region. Extracts prepared using 96% ethanol, 50% ethanol and hot distilled water were examined for phenolic composition, as well as for their antioxidant, enzyme-inhibiting and cytotoxic activities. The HPLC-DAD method was employed for quantitative-qualitative analysis of phenolic compounds in extracts, which confirmed the presence of rosmarinic and caffeic acids and luteolin-7-O-glucoside. Total phenolic and flavonoid contents, antioxidant activity, and inhibition of acetylcholinesterase and tyrosinase were measured spectrophotometrically. In the applied antioxidant tests (DPPH, FRAP and ?-carotene/linoleic acid tests) and tests of inhibition of the enzymes acetylcholinesterase and tyrosinase, the SOP extracts showed stronger antioxidant and enzyme-inhibiting activities compared to SOV and SOL, while the most efficient solvent was 50% ethanol. The highest cytotoxic activity in the MTT test was recorded for 96% ethanol extracts, especially in the case of the SOL sample, against the A375 cell line. All of the tested bioactivities were more strongly correlated with total phenolic content than with flavonoid content. Differences in the tested bioactivities of extracts obtained from plants collected at ecologically different localities and with different extraction solvents could be explained by variations in total phenolic and flavonoid contents.

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TLC–direct bioautography as a method for evaluation of antibacterial properties of Thymus vulgaris L. and Salvia officinalis L. essential oils of different origin

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826076.2017.1298031 ◽

2017 ◽

Vol 40 (5-6) ◽

pp. 292-296 ◽

Cited By ~ 9

Author(s):

Wioleta Jesionek ◽

Barbara Majer-Dziedzic ◽

Irena M. Choma

Keyword(s):

Essential Oils ◽

Antibacterial Properties ◽

Salvia Officinalis ◽

Thymus Vulgaris ◽

Direct Bioautography ◽

Salvia Officinalis L ◽

Different Origin

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Antimicrobial effects of essential oils from Tanacetum vulgare L. and Salvia officinalis L., growing in Slovakia

Nova Biotechnologica et Chimica ◽

10.36547/nbc.1273 ◽

2021 ◽

Vol 9 (2) ◽

Author(s):

Mária Mikulášová ◽

Štefánia Vaverková

Keyword(s):

Essential Oils ◽

Antimicrobial Properties ◽

Salvia Officinalis ◽

Diffusion Method ◽

Tanacetum Vulgare ◽

Gram Positive Bacteria ◽

Microdilution Method ◽

Agar Diffusion Method ◽

Salvia Officinalis L ◽

Total Oil

Possible antimicrobial properties of essential oils isolated from Tanacetum vulgare L., and Salvia officinalis L., harvested from five different locations in Slovakia, were examined using the disc agar diffusion method and by the microdilution method. GC/MS analysis of the essential oil from Tanacetum vulgare L. resulted in the identification of 16 compounds constituting 82.1% of the total oil. Gram-positive bacteria, mainly Bacillus subtilis, were more susceptible to essential oils from both plants than were gramnegative species. Tested essential oils posses also anti-yeast activity. The shares of the constituents in the essential oils as well as their antimicrobial activity differed in dependence on the locality.

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Synergistic Antifungal, Allelopatic and Anti-Proliferative Potential of Salvia officinalis L., and Thymus vulgaris L. Essential Oils

Molecules ◽

10.3390/molecules23010185 ◽

2018 ◽

Vol 23 (1) ◽

pp. 185 ◽

Cited By ~ 14

Author(s):

Ersilia Alexa ◽

Renata Sumalan ◽

Corina Danciu ◽

Diana Obistioiu ◽

Monica Negrea ◽

...

Keyword(s):

Essential Oils ◽

Salvia Officinalis ◽

Thymus Vulgaris ◽

Proliferative Potential ◽

Salvia Officinalis L

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Chemical composition of essential oils from leaves and twigs with leaves of two new varieties of common sage (Salvia officinalis L.)

Planta Medica ◽

10.1055/s-0030-1264353 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

A Aprotosoaie ◽

E Gille ◽

A Spac ◽

M Gonceariuc ◽

M Hancianu ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Salvia Officinalis ◽

New Varieties ◽

Salvia Officinalis L

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Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

Revista de Chimie ◽

10.37358/rc.19.6.7266 ◽

2019 ◽

Vol 70 (6) ◽

pp. 2015-2020

Author(s):

Silvia Robu ◽

Aurelia Romila ◽

Olimpia Dumitriu Buzia ◽

Adrian Florin Spac ◽

Camelia Diaconu ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Plant Material ◽

Mobile Phase ◽

Chromatographic Method ◽

Salvia Officinalis ◽

Salvia Officinalis L ◽

Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

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An investigation of the effect of Zataria multiflora Boiss and Mentha piperita essential oils to improve the chemical stability of minced meat

Veterinary World ◽

10.14202/vetworld.2018.1656-1662 ◽

2018 ◽

pp. 1656-1662 ◽

Cited By ~ 1

Author(s):

Mojtaba Raeisi ◽

Mohammad Hashemi ◽

Majid Aminzare ◽

Asma Afshari ◽

Tayebeh Zeinali ◽

...

Keyword(s):

Treatment Group ◽

Essential Oils ◽

Chemical Stability ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Mentha Piperita ◽

Total Phenolic ◽

Control Group ◽

Zataria Multiflora ◽

Minced Meat

Background and Aim: Extending the shelf life of foods is an essential concept in food safety. Most of the time, foods deteriorate through the growth of microorganisms or oxidation process. Essential oils (EOs) derived from plant material have well-documented antioxidant and antibacterial activity. This study aimed to evaluate the effect of Zataria multiflora Boiss EO (ZEO) and Mentha piperita EO (MEO) on the chemical stability of minced meat during storage at 7°C. Materials and Methods: Total phenolic content, β-Carotene bleaching test, ferric reducing antioxidant potential assay, and 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity were used to determine the antioxidant potential of EOs. Five different groups including control, ZEO 0.3%, ZEO 0.5%, MEO 0.3%, and MEO 0.5% were designed to assess the chemical stability of minced meat by measuring pH, thiobarbituric acid (TBA), total volatile base nitrogen (TVBN), and peroxide value (PV). Results: pH did not have any significant change during storage. TBA values in the control group were significantly higher than the treatment groups, especially from the 5th day of storage. TVBN in the treatment group was significantly lower than the control group during storage. PV values in the treatment group were significantly lower than the control group during storage. Conclusion: Results indicate that ZEO and MEO had an excellent antioxidant activity and retarded the spoilage process in minced meat. Keywords: antioxidant, Mentha piperita, minced meat, Zataria multiflora Boiss.

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A study of anti fungal activity of a combination of essential oils from medical herbs against water molds

Baghdad Science Journal ◽

10.21123/bsj.13.4.674-680 ◽

2016 ◽

Vol 13 (4) ◽

pp. 674-680

Author(s):

Baghdad Science Journal

Keyword(s):

Essential Oils ◽

Common Carp ◽

Hplc Analysis ◽

Salvia Officinalis ◽

Active Compounds ◽

Fungal Activity ◽

Salvia Officinalis L ◽

Anti Fungal

The aim of this study is to evaluate the anti fungal activity of a combination of essential oils against water molds. HPLC analysis was done to evaluate the quantity and quality of the active compounds in this combination which extracted from three herbs( Peppermint Menthapiperita ,Thyme Thymusvulgaris, Common sage Salvia officinalis L.) and the active compounds are Camphor,Menthol,,Thujone and Thymol with different concentrations. In this study (MIC) , (MFC) were measured and (LD50) determined after 48,96 h from fingerlings treatment of common carp in aquariums .The results of (MIC) were 0.025µl/ml for Aphanomyces sp. and 0.015µl/ml for both Achlya sp. and Fusariumsolani which showed significant differences(p

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In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections

Antibiotics ◽

10.3390/antibiotics9050272 ◽

2020 ◽

Vol 9 (5) ◽

pp. 272 ◽

Cited By ~ 1

Author(s):

Ramona Iseppi ◽

Alessandro Di Cerbo ◽

Piero Aloisi ◽

Mattia Manelli ◽

Veronica Pellesi ◽

...

Keyword(s):

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Essential Oils ◽

Disk Diffusion ◽

Mentha Piperita ◽

Thymus Vulgaris ◽

Extended Spectrum ◽

Disk Diffusion Assay ◽

Diffusion Assay

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), Melaleuca alternifolia, Eucalyptus globulus, Mentha piperita, and Thymus vulgaris, in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and carbapenemase (KPC)-producing Klebsiella pneumoniae. A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing E. coli, 5 K. pneumoniae, 13 KPC-producing K. pneumoniae, and 20 MBL-producing P. aeruginosa were cultured and reconfirmed as ESBL and carbapenamase producers. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (ESBL and KPC/MBL). Antibacterial activity of the EOs was determined using the agar disk diffusion assay, and minimal inhibitory concentrations (MICs) were also evaluated. Lastly, adhesion capability and biofilm formation on polystyrene and glass surfaces were studied in 24 randomly selected strains. M. alternifolia and T. vulgaris EOs showed the best antibacterial activity against all tested strains and, as revealed by agar disk diffusion assay, M. alternifolia was the most effective, even at low concentrations. This effect was also confirmed by MICs, with values ranging from 0.5 to 16 µg/mL and from 1 to 16 µg/mL, for M. alternifolia and T. vulgaris EOs, respectively. The EOs’ antibacterial activity compared to antibiotics confirmed M. alternifolia EO as the best antibacterial agent. T. vulgaris EO also showed a good antibacterial activity with MICs lower than both reference antibiotics. Lastly, a significant anti-biofilm activity was observed for the two EOs (*P < 0.05 and **P < 0.01 for M. alternifolia and T. vulgaris EOs, respectively). A good antibacterial and anti-biofilm activity of M. alternifolia and T. vulgaris EOs against all selected strains was observed, thus demonstrating a future possible use of these EOs to treat infections caused by ESBL/carbapenemase-producing strains, even in association with antibiotics.

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