Genotoxic Effects of Dental Panoramic Radiograph in Children

2010 ◽  
Vol 35 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Eman El-Ashiry ◽  
Eman Abo-Hager ◽  
Abeer Gawish

This study was completed to evaluate chromosomal damage (micronucleus) and cellular death in exfoliated buccal mucosa cells taken from healthy children following exposure to panoramic radiation during dental radiography. Method: Twenty children who underwent panoramic dental radiography for diagnostic purposes were included. Cytological preparations were stained with Feulgen stain, identified under light microscopy. Micronuclei, apoptotic nuclear alterations (condensed chromatin, karyorrhexis, pyknosis) and necrosis (karyolysis) were scored. Results showed no statistically significant differences in children's micronucleated oral mucosa cells before and after panoramic dental X-Ray exposure. On the other hand,there was a statistically significant increase in nuclear alterations closely related to genotoxicity such as condensed chromatin, karyorrhexis and pyknosis, while karyolysis of oral mucosal cells did not show significant increase after panoramic X-Ray exposure. Conclusion: Dental panoramic radiography may not be a factor that induces chromosomal damage, but is able to promote genotoxicity in children.

2012 ◽  
Vol 83 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Diego Coelho Lorenzoni ◽  
Ana Carolina Cuzzuol Fracalossi ◽  
Viviane Carlin ◽  
Daniel Araki Ribeiro ◽  
Eduardo Franzotti Sant'Anna

Abstract Objectives: To evaluate and compare mutagenicity (micronucleus) and cytotoxicity (karyorrhexis, pyknosis, and karyolysis) in exfoliated buccal mucosa cells of children following cone beam computed tomography (CBCT) or conventional radiograph exposure necessary for orthodontic planning. Materials and Methods: A total of 49 healthy children were submitted to CBCT or a conventional orthodontic radiographic protocol; they were divided into two groups based on exam: CBCT (n  =  24) and Radiographic Set (n  =  25) groups. The micronucleus test in the exfoliated buccal mucosa cells was applied. Results: There was not a statistically significant difference (P > .05) found between the number of micronucleated buccal mucosa cells (MNC) before and after exposure to radiation in either group, showing that neither group experienced a mutagenic effect. However, radiation did cause other nuclear alterations closely related to cytotoxicity, including karyorrhexis, pyknosis, and karyolysis, in both groups (P < .05). The CBCT group presented a greater increase in cell death than was noted in the Radiographic Set group (P < .044). Conclusion: According to the micronucleus test, mutagenicity was not induced by the CBCT or the conventional radiographs, but cytotoxicity was verified after these exams, especially after CBCT. That might have happened once the CBCT group received a greater radiation dose than the Radiographic Set group as a result of the protocols used in orthodontic planning for this study.


Author(s):  
T. Gulik-Krzywicki ◽  
M.J. Costello

Freeze-etching electron microscopy is currently one of the best methods for studying molecular organization of biological materials. Its application, however, is still limited by our imprecise knowledge about the perturbations of the original organization which may occur during quenching and fracturing of the samples and during the replication of fractured surfaces. Although it is well known that the preservation of the molecular organization of biological materials is critically dependent on the rate of freezing of the samples, little information is presently available concerning the nature and the extent of freezing-rate dependent perturbations of the original organizations. In order to obtain this information, we have developed a method based on the comparison of x-ray diffraction patterns of samples before and after freezing, prior to fracturing and replication.Our experimental set-up is shown in Fig. 1. The sample to be quenched is placed on its holder which is then mounted on a small metal holder (O) fixed on a glass capillary (p), whose position is controlled by a micromanipulator.


Author(s):  
R. M. Anderson

Aluminum-copper-silicon thin films have been considered as an interconnection metallurgy for integrated circuit applications. Various schemes have been proposed to incorporate small percent-ages of silicon into films that typically contain two to five percent copper. We undertook a study of the total effect of silicon on the aluminum copper film as revealed by transmission electron microscopy, scanning electron microscopy, x-ray diffraction and ion microprobe techniques as a function of the various deposition methods.X-ray investigations noted a change in solid solution concentration as a function of Si content before and after heat-treatment. The amount of solid solution in the Al increased with heat-treatment for films with ≥2% silicon and decreased for films <2% silicon.


2011 ◽  
Vol 314-316 ◽  
pp. 273-278
Author(s):  
Yu Hua Dong ◽  
Ke Ren ◽  
Qiong Zhou

Linear low density polyethylene (LLDPE) was chemically modified with grafting maleic anhydride (MAH) monomer on its backbone by melting blending. Nano-particles SiO2 was modified by cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and anionic surfactant sulfosalicylic acid (SSA) and added to PE coating respectively. Measurement of membrane potential showed that the coating containing modified SiO2 nano-particles had characteristic of ion selectivity. The properties of the different coatings were investigated according to relative industrial standards. Experimental results indicated that PE coating with ion selectivity had better performances, such as adhesion strength, cathodic disbonding and anti-corrosion, than those of coating without ion selectivity. Crystal structure of the coatings before and after alkali corrosion was characterized by Fourier transform infrared spectra (FTIR) and X-ray diffraction (XRD). Structure of the coating without ion selectivity was damaged by NaOH alkali solution, causing mechanical properties being decreased. And the structure of the ion selective coatings was not affected.


Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3588
Author(s):  
Jiayi Chen ◽  
Yansong Liu ◽  
Jiayue Zhang ◽  
Yuanlin Ren ◽  
Xiaohui Liu

Lyocell fabrics are widely applied in textiles, however, its high flammability increases the risk of fire. Therefore, to resolve the issue, a novel biomass-based flame retardant with phosphorus and nitrogen elements was designed and synthesized by the reaction of arginine with phosphoric acid and urea. It was then grafted onto the lyocell fabric by a dip-dry-cure technique to prepare durable flame-retardant lyocell fabric (FR-lyocell). X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated that the flame retardant was successfully introduced into the lyocell sample. Thermogravimetric (TG) and Raman analyses confirmed that the modified lyocell fabric featured excellent thermal stability and significantly increased char residue. Vertical combustion results indicated that FR-lyocell before and after washing formed a complete and dense char layer. Thermogravimetric Fourier-transform infrared (TG-FTIR) analysis suggested that incombustible substances (such as H2O and CO2) were produced and played a significant fire retarding role in the gas phase. The cone calorimeter test corroborated that the peak of heat release rate (PHRR) and total heat release (THR) declined by 89.4% and 56.4%, respectively. These results indicated that the flame retardancy of the lyocell fabric was observably ameliorated.


Author(s):  
F. Mostefa ◽  
Nasr Eddine Bouhamou ◽  
H.A. Mesbah ◽  
Salima Aggoun ◽  
D. Mekhatria

This work aims to study the feasibility of making a geopolymer cement based on dredged sediments, from the Fergoug dam (Algeria) and to evaluate their construction potential particularly interesting in the field of special cementitious materials. These sediments due to their mineralogical composition as aluminosilicates; are materials that can be used after heat treatment. Sedimentary clays were characterized before and after calcination by X-ray diffraction, ATG / ATD, spectroscopy (FTIR) and XRF analysis. The calcination was carried out on the raw material sieved at 80 μm for a temperature of 750 ° C, for 3.4 and 5 hours. The reactivity of the calcined products was measured using isothermal calorimetric analysis (DSC) on pastes prepared by mixing an alkaline solution of sodium hydroxide (NaOH) 8 M in an amount allowing to have a Na / Al ratio close to 1 (1: 1). Also, cubic mortar samples were prepared with a ratio L / S: 0.8, sealed and cured for 24 hours at 60 ° C and then at room temperature until the day they were submited to mechanical testing. to check the extent of geopolymerization. The results obtained allowed to optimize the calcination time of 5 hours for a better reactivity of these sediments, and a concentration of 8M of sodium hydroxide and more suitable to have the best mechanical performances.


1995 ◽  
Vol 41 (1) ◽  
pp. 33-42
Author(s):  
Keizo UMEGAKI ◽  
Saishi HIROTA ◽  
Mari AOSHIMA ◽  
Shigeshi AOKI ◽  
Takatoshi ESASHI

1998 ◽  
Vol 13 (5) ◽  
pp. 1238-1242 ◽  
Author(s):  
T. Battault ◽  
R. Legros ◽  
A. Rousset

“Aging,” defined as the drift of resistance with temperature after 1000 h, was investigated for iron manganite temperature coefficient thermistors. For these devices, aging is relatively large, about 10%. The cationic distributions before and after aging were determined by Mössbauer spectroscopy. These distributions explain all the x-ray diffraction and correlated electrical data. The origin of the aging observed on iron manganites thermistors has been identified. It is due to the migration of Fe3+ ions from tetrahedral to octahedral sites of the spinel structure in order to reach a structural equilibrium.


Sign in / Sign up

Export Citation Format

Share Document