Caries Protective Agents in Human Milk and Bovine Milk: An in vitro Study

2011 ◽  
Vol 35 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Vabitha Shetty ◽  
Amitha Hegde ◽  
Nandan S ◽  
Suchetha Shetty
Keyword(s):  
Human Milk ◽  
Whey Protein ◽  
Milk Fat ◽  
Bovine Milk ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Labor Ward ◽  
Calcium And Phosphorus ◽  
The Individual

Objectives: To estimate Calcium and Phosphorus withdrawal from hydroxyapatite in the presence of bovine milk and human milk from which the following protective fractions namely Casein, Whey protein, Lactose and Milk fat have been individually removed and to compare the above protective fractions in human and bovine milk. Method: Human milk obtained from lactating mothers in the labor ward of Kshema hospital was subjected to immediate analysis. Bovine milk was obtained from a local dairy. Equal quantities of human milk and bovine milk (1ml) were separately subjected to the systematic removal of the four milk fractions. As each fraction was removed, the remaining milk samples were subjected to testing. Powdered hydroxyapatite from human dental enamel was subjected to demineralization with the addition of the milk sample under test for 20 minutes. This mixture was then centrifuged. Aliquots of the supernatant were taken for calcium and Phosphorus analysis using photospectrometry. Ten demineralization tests were similarly carried out for every milk fraction for both human and bovine milk separately. Equal samples of whole bovine milk and whole human milk were also subjected to similar testing. Results: The calcium and phosphorus dissolution values were higher when the individual fractions were eliminated from both human milk/enamel samples and bovine milk/enamel samples as compared to the values obtained from whole human milk/whole bovine milk /enamel samples. Further, higher calcium and phosphorus dissolution values were observed when the fractions were individually and separately removed from the whole human milk/enamel samples as compared to the corresponding values obtained when these fractions were removed from bovine milk/enamel samples. Conclusion: The evaluated milk fraction in bovine milk namely casein,whey protein, lactose and milk fat were individually more caries protective when compared to the corresponding fractions in human milk.

scholarly journals Correction: Free fatty acid release from vegetable and bovine milk fat-based infant formulas and human milk during two-phase in vitro digestion

2019 ◽  
Vol 10 (5) ◽  
pp. 3018-3020
Author(s):  
Jeske H. J. Hageman ◽  
Jaap Keijer ◽  
Trine Kastrup Dalsgaard ◽  
Lara W. Zeper ◽  
Frédéric Carrière ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Human Milk ◽  
Milk Fat ◽  
Bovine Milk ◽  
Infant Formulas ◽  
Two Phase ◽  
Fatty Acid Release ◽  
Acid Release

Correction for ‘Free fatty acid release from vegetable and bovine milk fat-based infant formulas and human milk during two-phase in vitro digestion’ by Jeske H. J. Hageman et al., Food Funct., 2019, 10, 2102–2113.

scholarly journals Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 410 ◽  
Author(s):  
Kristine Bach Korsholm Knudsen ◽  
Christine Heerup ◽  
Tine Røngaard Stange Jensen ◽  
Xiaolu Geng ◽  
Nikolaj Drachmann ◽  
...  
Keyword(s):  
Milk Fat ◽  
Bovine Milk ◽  
In Vitro Digestion ◽  
Lipid Absorption ◽  
Lipid Digestion ◽  
Soy Lecithin ◽  
Neonatal Piglets ◽  
Milk Fat Globule Membranes

Efficient lipid digestion in formula-fed infants is required to ensure the availability of fatty acids for normal organ development. Previous studies suggest that the efficiency of lipid digestion may depend on whether lipids are emulsified with soy lecithin or fractions derived from bovine milk. This study, therefore, aimed to determine whether emulsification with bovine milk-derived emulsifiers or soy lecithin (SL) influenced lipid digestion in vitro and in vivo. Lipid digestibility was determined in vitro in oil-in-water emulsions using four different milk-derived emulsifiers or SL, and the ultrastructural appearance of the emulsions was assessed using electron microscopy. Subsequently, selected emulsions were added to a base diet and fed to preterm neonatal piglets. Initially, preterm pigs equipped with an ileostomy were fed experimental formulas for seven days and stoma output was collected quantitatively. Next, lipid absorption kinetics was studied in preterm pigs given pure emulsions. Finally, complete formulas with different emulsions were fed for four days, and the post-bolus plasma triglyceride level was determined. Milk-derived emulsifiers (containing protein and phospholipids from milk fat globule membranes and extracellular vesicles) showed increased effects on fat digestion compared to SL in an in vitro digestion model. Further, milk-derived emulsifiers significantly increased the digestion of triglyceride in the preterm piglet model compared with SL. Ultra-structural images indicated a more regular and smooth surface of fat droplets emulsified with milk-derived emulsifiers relative to SL. We conclude that, relative to SL, milk-derived emulsifiers lead to a different surface ultrastructure on the lipid droplets, and increase lipid digestion.

scholarly journals SEM Analysis of Enamel Abrasion after Air Polishing Treatment with Erythritol, Glycine and Sodium Bicarbonate

Coatings ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 549 ◽  
Author(s):  
Bruna Sinjari ◽  
Gianmaria D’Addazio ◽  
Martina Bozzi ◽  
Manlio Santilli ◽  
Tonino Traini ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Sodium Bicarbonate ◽  
Air Flow ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Medical Systems ◽  
Air Polishing ◽  
And Control

The aim of this in vitro study was to evaluate the enamel surface topography after treatment with three air polishing powders: Glycine (A), erythritol (B), and sodium bicarbonate (C) (Air Flow Soft, Plus and Classic powders, EMS Electro Medical Systems S.A., Nyon, Switzerland). Fifteen extracted incisors were randomly divided into three groups of five teeth each, A, B and C, respectively. The teeth were blocked in plaster bases, washed, dried and half-covered with polytetrafluoroethylene strips before treatment. In this way, each half-treated dental element became test and control of itself. Comparative statistical analysis of Rq (geometric average of the deviations occurring in roughness profile) was performed. The scanning electron microscope (SEM) analysis showed different degrees of surface roughness between the groups, decreasing after treatment. In addition, a statistically significant reduction p < 0.05 was present in group C (Rq mean non-treated 108.17 µm, 95% CI: 97.29–124.01 and Rq mean treated 86.78 µm, 95% CI: 80.63–93.70). A decrease in surface roughness post-treatment was not observed in group A and B. Therefore, it may be concluded that the air flow powders tested herein can be used on dental enamel to reduce the surface roughness due to function and the action of dental therapies.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

scholarly journals Comparison of Oxidative Status of Human Milk, Human Milk Fortifiers and Preterm Infant Formulas

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 458 ◽  
Author(s):  
Pozzo ◽  
Cirrincione ◽  
Russo ◽  
Karamać ◽  
Amarowicz ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Preterm Infant ◽  
Bovine Milk ◽  
Growth Failure ◽  
Postnatal Growth ◽  
Infant Formulas ◽  
Low Birth Weight Infants ◽  
Multivariate Statistical

Preterm and low birth weight infants require specific nutrition to overcome the accumulated growth deficit, and to prevent morbidities related to postnatal growth failure. In order to guarantee an adequate nutrient-intake, mother’s own milk, when available, or donor human milk, are usually fortified with additional nutrients, in particular proteins. Fortification with processed ingredients may result in additional intake in oxidative compounds, deriving from extensive heat treatments, that are applied during processing. The aim of the present work was to compare the in vitro antioxidant activity and oxidative compound content conveyed by different preterm infant foods and fortifiers, namely raw and pasteurized human milk, two different preterm infant formulas, three bovine milk-based fortifiers and two experimental donkey milk-based fortifiers. Univariate and multivariate statistical analyses revealed significant differences between the different products. The use of human milk minimizes the intake of dietary oxidative compound in comparison to infant formulas, irrespective of pasteurization or fortification, especially as far as malondialdehyde content is concerned. The addition of fortifiers to human milk increases its antioxidant capacity, and the choice of the protein source (hydrolysed vs. whole proteins) differently impacted the resulting total antioxidant capacity of the diet.

Influence of Homogenization and Thermal Processing on the Gastrointestinal Fate of Bovine Milk Fat: In Vitro Digestion Study

2017 ◽  
Vol 65 (50) ◽  
pp. 11109-11117 ◽  
Author(s):  
Li Liang ◽  
Ce Qi ◽  
Xingguo Wang ◽  
Qingzhe Jin ◽  
David Julian McClements
Keyword(s):  
Thermal Processing ◽  
Milk Fat ◽  
Bovine Milk ◽  
In Vitro Digestion

scholarly journals Comparison of Shear Bond Strength of MIM Technology Brackets with Conventional and Rail-Shaped Mesh Bases: An In Vitro Study

2019 ◽  
Vol 13 (1) ◽  
pp. 255-260
Author(s):  
Francisco Molina ◽  
Karina Maria Salvatore Freitas ◽  
Maria Cristina Rockenbach Binz Ordóñez ◽  
Emerson Flamarion Cruz ◽  
Rafael Pinelli Henriques ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Metal Injection Molding ◽  
Test Machine ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Mim Technology

Objective: The objective of this study was to evaluate in vitro the shear bond strength of two types of MIM (Metal Injection Molding) technology brackets, one with conventional mesh base and the other with rail-shaped mesh base. Materials and Methods: Forty human premolars received the bonding of 2 types of brackets: Group 1- 20 Synergy metal brackets (Rocky Mountain) with conventional mesh base and Group 2-20 H4 brackets (OrthoClassic) with rail-shaped mesh base. Both brackets were bonded with Resilience photopolymerizable resin (OrthoTechnology). The specimens were coupled to a Tinius Olsen universal test machine where the shear test was performed using a chisel. In addition, the amount of remaining resin in tooth crown with the ImageJ program was evaluated and the Adhesive Remnant Index (ARI). Intergroup comparison was performed by the independent t test and Chi-square test. Results: There was no statistically significant difference between the groups for any of the measures evaluated indicating that the mesh type of the brackets’ base with MIM technology did not influence the shear bond strength of the brackets (shear bond strength, p=0.191; maximum load registered, p=0.244). There was also no difference between the percentage (p=0.602) and area of remaining resin in the teeth (p=0.805) and IRA (p=0.625) between the Synergy and H4 groups. Conclusion: Shear bond strength was similar in the two types of brackets with MIM technology evaluated. In addition, the remaining resin in the dental enamel of two types of brackets were also similar.

scholarly journals The effect of feeding diets markedly differing in the proportion of grass and maize silages on bovine milk fat composition

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
E. Samková ◽  
M. Pešek ◽  
J. Špička ◽  
T. Pelikánová ◽  
O. Hanuš
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Saturated Fatty Acids ◽  
Bovine Milk ◽  
Atherogenic Index ◽  
Maize Silage ◽  
Grass Silage ◽  
Lactation Stage ◽  
Fat Composition ◽  
The Individual

Ten Czech Pied cows in the mid-lactation stage were fed diets based on grass silage and maize silage. The composition of milk fats differed. The proportions of even-chain saturated fatty acids (SFAs) up to C<sub>14:0</sub> were insignificant and the content of C<sub>16:0</sub> was significantly higher (<i>P</i> < 0.05) when feeding a diet based on maize silage, while the proportions of the individual polyunsaturated fatty acids (PUFAs) were significantly (except for C<sub>18:2</sub>) higher when feeding a diet based on grass silage. The total SFA proportions were 67.60 and 62.93% (<i>P</i> < 0.05) of maize and grass silages, respectively, while an opposite relation was observed for the sum of PUFAs (3.56 and 4.74%; <i>P</i> < 0.001). Feeding of grass silage resulted in a significantly lower proportion of hypercholesterolaemic fatty acids C<sub>12:0</sub>, C<sub>14:0</sub> and C<sub>16:0</sub> (49.38 and 44.98%, respectively; <i>P</i> < 0.05) and in lower values of the atherogenic index (3.03 and 2.44; <i>P</i> < 0.05). Thus, the results could be used for the improvement of milk fat composition.

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