scholarly journals BIOCHEMICAL APPROACH TO STRUCTURING THE VARIETY DIVERSITY OF THE NAKED AND COVERED FORMS OF CULTURAL OATS (AVENA SATIVA L.)

2019 ◽  
Author(s):  
Igor Loskutov ◽  
Tatiana Shelenga ◽  
Aleksei Konarev ◽  
Yulia Vargach ◽  
Elizaveta Porokhovinova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Genetic Diversity ◽  
Avena Sativa ◽  
Fatty Acid Content ◽  
Principal Component ◽  
Nutritional Composition ◽  
Biologically Active ◽  
Similar Proportion ◽  
Gas Liquid Chromatography ◽  
Development Of Resistance

Background. Structuring and phenotyping genetic diversity is an important aspect of the work with breeding sources and materials. In the Introduction, the authors pointed out the role of N.I. Vavilovs scientific foresight in defining the topical trend in researching the genetic diversity of a crop, particularly the analysis of its biochemical composition. As the target of their research, the authors chose biochemical characters identifiable in the process of metabolomic analysis conducted by means of gas chromatography with mass spectrometry. Materials and methods. The object was the grain of naked and covered forms of common oat (Avena sativa L.) from the collection held by the Oat, Rye and Barley Genetic Resources Department of VIR. The analysis of oil fatty acid content and metabolomic research were performed using the method of gas-liquid chromatography with mass spectrometry on the chromatograph Agilent 6850 (USA). Results. The obtained metabolomic spectra which reflected the metabolomic status of genotypes of various ecogeographic origin were compared among themselves using statistical (principal component) analysis methods. The results of the comparison are discussed by referring to the most important groups of metabolites significant for forming the traits of resistance to stressors as well as the characters related to food qualities of grain products. Special attention has been paid to biologically active compounds determining the functional value of the products for human nutrition: the sum of phenolics in covered forms is five times higher than that in naked ones and the content of glycine amino acid in covered forms is five times higher than in naked grain, with a similar proportion in the content of organic acids, sugars, etc. Conclusion. Differences between metabolomic profiles of naked and covered forms have been detected and statistically verified. Accessions with the most optimal nutritional composition have been identified for food purposes and for the development of resistance to biotic and abiotic environmental stresses.

scholarly journals Genetic Diversity In Nutritional Composition Of Oat (Avena Sativa L.) Germplasm Reported From Pakistan

2021 ◽  
Author(s):  
Mohammad Ihsan ◽  
Mohammad Nisar ◽  
Nausheen Nazir ◽  
Muhammad Zahoor ◽  
Atif Ali Khan Khalil ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Avena Sativa ◽  
Nutritional Composition

scholarly journals Genetic Diversity in Local and Exotic Avena sativa L. (Oat) Germplasm Using Multivariate Analysis

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1713
Author(s):  
Mohammad Ihsan ◽  
Nausheen Nazir ◽  
Abdul Ghafoor ◽  
Atif Khalil ◽  
Muhammad Zahoor ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Avena Sativa ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Human Consumption ◽  
Local Conditions ◽  
Yield Production ◽  
Sds Page ◽  
Poaceae Family ◽  
Elite Germplasm

Avena sativa L., also known as Oat belongs to the Poaceae family, is one of the most significant crops that is grown for its seeds, fodder as well as for human consumption as oatmeal. In the current study, 236 genotypes of A. sativa were analysed for genetic diversity through agro-morphological and SDS-PAGE analysis. Cluster analysis based on agro-morphological characteristics grouped all the genotypes into nine clusters, whereas genotype numbers 537 and 728 were highly different from others. The seed yield production of cluster 9 genotypes was the highest per plant (38.2 ± 0.20 g), while cluster 2 genotypes produced maximum biomass per plant (122.5 ± 9.55 g) as compared to other clusters. In a principal component analysis where four variables were studied, and the observed total variations were 57.60%. Among the genotypes, a maximum grain yield of 38.2 g (each) was recorded for genotypes 22,350 and 728, followed by genotypes 737 and 22,390 (with 36.4 g and 35.6 g of seed productions, respectively). The SDS-PAGE analysis resulted in 13 bands and all the genotypes were grouped into seventeen clusters. At the extreme periphery of the dendrogram, genotype 537 and 22,332 were considered to be the most diverse genotypes. Our findings have implications for both understanding the diversity and relationships among these diverse genotypes of A. sativa and will provide a basis for obtaining the elite germplasm optimally adapted to local conditions. The selected genotypes based on agronomic performance may be potential breeding material to raise successful future cultivars.

Neutral Lipids of Oats Fruit (Avena Sativa L.)

2020 ◽  
Vol 9 (4) ◽  
pp. 40-43
Author(s):  
N. K. Yuldasheva ◽  
S. D. Gusakova ◽  
D. Kh. Nurullaeva ◽  
N. T. Farmanova ◽  
R. P. Zakirova ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Neutral Lipids ◽  
The Body ◽  
Biologically Active ◽  
Absorption Bands ◽  
Vital Functions ◽  
The Republic ◽  
Living Organisms ◽  
Main Components ◽  
Active Substances

Introduction. Lipids are a widespread group of biologically active substances in nature, making up the bulk of the organic substances of all living organisms. They accumulate in plants in seeds, as well as in fruits and perform a number of vital functions: they are the main components of cell membranes and the energy reserve for the body.Aim. Study of neutral lipids of sown oats (Avena sativa L.).Materials and methods. The objects of the study were fruits (grains) of oats of the sown variety "Tashkent 1," harvested in the Republic of Uzbekistan. Results and discussions. Neutral lipids of oat grains have been found to contain 13 fatty acids with a predominance of the sum of oleic, linolenic and linoleic acids. The total degree of unsaturation was almost 78%. Absorption bands characteristic of these substances were observed in the IR spectrum of MEGC.Conclusion. According to the results of the NL analysis, oat grains consisted of triacylglycerides and free LCDs, which were accompanied by hydrocarbons, phytosterols, triterpenoids and tocopherols.

Genetic Analysis of Chinese Cucumber Collections in the U.S. National Germplasm Collection

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534e-534 ◽  
Author(s):  
J. Staub ◽  
Felix Sequen ◽  
Tom Horejsi ◽  
Jin Feng Chen
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Allelic Variation ◽  
Germplasm Collection ◽  
Principal Component ◽  
Isozyme Loci ◽  
National Plant Germplasm System ◽  
The U.S

Genetic variation in cucumber accessions from China was assessed by examining variation at 21 polymorphic isozyme loci. Principal component analysis of allelic variation allowed for the depiction of two distinct groupings of Chinese accessions collected in 1994 and 1996 (67 accessions). Six isozyme loci (Gpi, Gr, Mdh-2, Mpi-2, Pep-gl, and Pep-la) were important in elucidating these major groups. These groupings were different from a single grouping of Chinese 146 accessions acquired before 1994. Allelic variation in Chinese accessions allowed for comparisons with other accessions in the U.S. National Plant Germplasm System (U.S. NPGS) collection grouped by continent and sub-continent. When Chinese accessions taken collectively were compared with an array of 853 C. sativus U.S. NPGS accessions examined previously, relationships differed between accessions grouped by country or subcontinent. Data indicate that acquisition of additional Chinese and Indian cucumber accessions would be strategically important for increasing genetic diversity in the U.S. NPGS cucumber collection.

Multivariate Analysis, Genetic Diversity and Phenotypic Correlation of Nineteen Exotic Groundnut Accessions

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
YPJ AMARASINGHE ◽  
G . WIJESINGHE ◽  
R W PUSHPAKUMARA
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Growth Parameters ◽  
Principal Component ◽  
Phenotypic Correlation ◽  
Clustering Method ◽  
Yield Parameters ◽  
Arachis Hypogaea L ◽  
Semi Arid ◽  
Number Of Branches

19 Groundnut ( Arachis hypogaea L. ) genotypes receiv ed from International Crops Research Institute for Semi Arid T ropics (ICRISA T) India w ere ev aluated in a non replicated trial and the characters w ere subjected to multiv ariate analysis to study the v ariability within the genotypes. The first 5 axes of the principal component analysis captured 78% of the total v ariability and identified yield parameters such as number of pods per plant, pod w eight per plant and growth parameters such as number of branches per plant, plant spread, and pod characteristics as the characters contributing most to total v ariation. Phenotypic correlation analysis rev ealed that the yield has positiv e correlation with the characters such as number of pods per plant and number of branches per plant. W ards clustering method has grouped the genotypes into 3 distinct clusters. The results can be applied in order to strengthen the breeding program

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

scholarly journals Analytical Methods for Quantification and Identification of Intact Glucosinolates in Arabidopsis Roots Using LC-QqQ(LIT)-MS/MS

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Kourosh Hooshmand ◽  
Inge S. Fomsgaard
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Crude Extract ◽  
Matrix Effects ◽  
Critical Role ◽  
Reversed Phase ◽  
Biologically Active ◽  
Natural Soil ◽  
Chromatography Method ◽  
Arabidopsis Root

Glucosinolates are biologically active secondary metabolites in Brassicaceae plants that play a critical role in positive and negative interactions between plants and root-associated microbial communities. The aim of this study was to develop a reversed-phase liquid chromatography method to quantify and identify intact glucosinolates in the root of Arabidopsis thaliana (Arabidopsis) grown in non-sterile natural soil by using liquid chromatography-hybrid triple quadruple-linear ion trap (LC-QqQ(LIT)) mass spectrometry. The Synergi Fusion C18-based column was found to be effective for sufficient retention and separation of nine intact glucosinolates without the need for time-consuming desulfation or ion-pairing steps. Method validation results showed satisfactory inter-day and intra-day precision for all glucosinolates except for 4-hydroxyglucobrassicin. Good inter-day and intra-day accuracy and recovery results were observed for glucoiberin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrassicin. However, for 4-hydroxyglucobrassicin, glucoraphanin and glucoerucin corrections to quantification results might be necessary since the recovery and accuracy results were not optimal. Matrix effects were shown to have a negligible effect on the ionization of all target analytes. The established liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was applied to quantify target intact glucosinolates in Arabidopsis root crude extract of four different wild-type accessions where differences in terms of concentration and composition of intact glucosinolates were observed. Employment of sensitive and selective precursor ion survey scan of m/z 97 in combination with the information-dependent acquisition (IDA) of the enhanced product ion (EPI) dependent scan (Prec97-IDA-EPI) using LC-QqQ(LIT) provided high confidence in structural characterization of diverse intact glucosinolate profiles in complex Arabidopsis root crude extract.

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