scholarly journals Blood test using the "thick drop" method

2021 ◽  
Vol 18 (3) ◽  
pp. 50-54
Author(s):  
M. Cherokov

I hardly need to talk about that. how important is the timely and rapid recognition of infectious diseases such as relapsing fever and malaria. Since the causative agents of these diseases in a certain period of the disease are in the peripheral blood, the usual method of recognizing these diseases is a blood test.

Parasitology ◽  
2007 ◽  
Vol 135 (2) ◽  
pp. 203-215 ◽  
Author(s):  
T. R. RAFFEL ◽  
T. BOMMARITO ◽  
D. S. BARRY ◽  
S. M. WITIAK ◽  
L. A. SHACKELTON

SUMMARYGiven the worldwide decline of amphibian populations due to emerging infectious diseases, it is imperative that we identify and address the causative agents. Many of the pathogens recently implicated in amphibian mortality and morbidity have been fungal or members of a poorly understood group of fungus-like protists, the mesomycetozoans. One mesomycetozoan, Amphibiocystidium ranae, is known to infect several European amphibian species and was associated with a recent decline of frogs in Italy. Here we present the first report of an Amphibiocystidium sp. in a North American amphibian, the Eastern red-spotted newt (Notophthalmus viridescens), and characterize it as the new species A. viridescens in the order Dermocystida based on morphological, geographical and phylogenetic evidence. We also describe the widespread and seasonal distribution of this parasite in red-spotted newt populations and provide evidence of mortality due to infection.


2017 ◽  
Vol 37 (11) ◽  
pp. 1327-1330
Author(s):  
Sabrina D.E. Campos ◽  
Camila S.C. Machado ◽  
Tatiana V.T. de Souza ◽  
Renan C. Cevarolli ◽  
Nádia R.P. Almosny

ABSTRACT: Populations of green-winged saltators, Saltator similis, are decreasing especially because of illegal trade and infectious diseases. We describe natural cases of an extraintestinal isosporoid coccidian in caged S. similis, and suggest the need of preventive measures in handling these birds. Nonspecific clinical signs were seen in all of them, however, intracytoplasmic Atoxoplasma sp. was found in peripheral blood, reinforcing the idea of systemic isosporosis. Leukocytosis with high number of heterophils and monocytes suggested that atoxoplasmosis in green-winged saltators can progress as an acute disease. The birds showed clinical improvement after treatment. Handling recommendations were proposed to upgrade hygienic conditions of the facilities. We concluded that nonspecific symptoms and an acute inflammatory process can be associated with atoxoplasmosis in young S. similis. We emphasize the importance of blood smear to detect merozoites.


2021 ◽  
Vol 9 (8) ◽  
pp. 1758
Author(s):  
Ines Cordeiro Filipe ◽  
Mariana Soares Guedes ◽  
Evgeny M. Zdobnov ◽  
Caroline Tapparel

Enteroviruses (EVs) from the D species are the causative agents of a diverse range of infectious diseases in spite of comprising only five known members. This small clade has a diverse host range and tissue tropism. It contains types infecting non-human primates and/or humans, and for the latter, they preferentially infect the eye, respiratory tract, gastrointestinal tract, and nervous system. Although several Enterovirus D members, in particular EV-D68, have been associated with neurological complications, including acute myelitis, there is currently no effective treatment or vaccine against any of them. This review highlights the peculiarities of this viral species, focusing on genome organization, functional elements, receptor usage, and pathogenesis.


PEDIATRICS ◽  
1972 ◽  
Vol 50 (5) ◽  
pp. 833-833
Author(s):  
John D. Nelson

Almost two years ago a group of eminent international authorities in the field of infectious diseases gathered near Cologne, Germany, for a week of reflection and discussion concerning the changing patterns of bacterial infections in recent decades and the possible reasons for the changes. The United States was represented by Drs. M. Finland and E. H. Kass of Boston, F. Daschner of Los Angeles, and A. von Graevenitz of New Haven. Other scientists were from Germany, France, Sweden, Great Britain, Switzerland, and Denmark.


2013 ◽  
Vol 6 ◽  
pp. CGast.S11858 ◽  
Author(s):  
Asit Ranjan Ghosh

The human body is host to a number of microbes occurring in various forms of host-microbe associations, such as commensals, mutualists, pathogens and opportunistic symbionts. While this association with microbes in certain cases is beneficial to the host, in many other cases it seems to offer no evident benefit or motive. The emergence and re-emergence of newer varieties of infectious diseases with causative agents being strains that were once living in the human system makes it necessary to study the environment and the dynamics under which this host microbe relationship thrives. The present discussion examines this interaction while tracing the origins of this association, and attempts to hypothesize a possible framework of selective pressures that could have lead microbes to inhabit mammalian host systems.


Parasitology ◽  
1915 ◽  
Vol 8 (1) ◽  
pp. 128-132 ◽  
Author(s):  
H. B. Fantham ◽  
Annie Porter

While engaged in experimental researches on the introduction of insect flagellates, belonging to the genera Herpetomonas and Crithidia, into vertebrates, we have recalled to mind some former observations of ours made in 1909. When examining the blood of mice artificially infected with the spirochaetes of relapsing fever in the Quick Laboratory, Cambridge, we observed flagellates in the peripheral blood of the mice. These flagellates, which were scanty, were studied in fresh preparations by us and drawings of the organism were made with the camera lucida. Some of these drawings are now reproduced, together with others made by us subsequently. The flagellates were also observed by Professor Nuttall and his laboratory assistant, Mr B. G. Clarke, and we were unanimously agreed that the flagellates belonged to the genus Herpetomonas, or Leptomonas as some have since preferred to call it. The significance of the presence of herpetomonads in the blood of mice was very puzzling at the time, and pressure of other work prevented the subject being followed up. During 1911 and 1912, these flagellate organisms have been seen by all of us, but they were always evanescent and could not be found in fixed and stained preparations. The parasites were seen on different occasions in three mice altogether.


2014 ◽  
Vol 19 (1) ◽  
pp. 34-44
Author(s):  
O. S. Bondareva ◽  
S. S. Savchenko ◽  
G. A. Tkachenko ◽  
A. I. Abueva ◽  
Yu. O. Muratova ◽  
...  

Currently genotyping of microorganisms is widely used in the investigation of outbreaks of infectious diseases, the implementation of epidemiological surveillance of infections and phylogenetic analysis of bacterial pathogens. Development of methods for genotyping is particularly topical for pathogens of such highly dangerous infections as plague, cholera, anthrax, brucellosis, tularemia, glanders and melioidosis, due to their high pathogenicity and contagiousness. In this overview there is presented the characteristics of different genotyping methods together with an indication of their advantages and drawbacks. There has been analyzed the frequency of the use of genotyping methods on an annual basis and in terms of the type of the causative agents of especially dangerous infections.


2021 ◽  
Vol 20 (2) ◽  
pp. 64-67
Author(s):  
M. N. Kuzmina ◽  
E. G. Klimovitskaya ◽  
S. N. Eshmolov ◽  
I. G. Sitnikov ◽  
E. V. Elyakova

The new coronavirus infection COVID-1 9 in children generally proceeds favorably, but in combination with other acute infectious diseases and in persons with background pathology and impaired immune reactivity may pose a particular threat.The article presents a clinical case of chickenpox, complicated by meningoencephalitis and cerebral edema, in combination with COVID-1 9 in a 5-year-old child. Chickenpox was not quite typical: against the background of febrile temperature, there was a scanty spot-papular rash with single vesicles that appeared later, the absence of elements on the mucous membranes and the development of neurological symptoms in the first day of the disease. CОVID-1 9 was characterized by a wave-like course with fever up to 39,1 °C, tonsillitis phenomena and inflammatory changes in the blood test.


2010 ◽  
Vol 10 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Dilista Piljić ◽  
Dragan Piljić ◽  
Sead Ahmetagić ◽  
Farid Ljuca ◽  
Humera Porobić-Jahić

Urinary tract infections (UTI) cause a great number of morbidity and mortality. These infections are serious complications in pregnancy, patients with diabetes, polycystic kidneys disease, sickle cell anaemia, kidney transplant and in patients with functional or structural anomalies of the urinary tract. The aim of this investigation was to determine a dominant causative agents of UTI and some of the clinical and laboratory characteristics of acute community-acquired UTI in adult hospitalised patients. We studied 200 adult patients with acute community-acquired UTI hospitalised in the Clinic for Infectious Diseases Tuzla from January 2006 to December 2007. The patients were divided into two groups: a group of patients with E. coli UTI (147) and a group of patients with non-E. coli UTI (53). In these two groups, the symptoms and signs of illness, blood test and urine analysis results were analysed. Our results have shown that the patients with E. coli UTI frequently had fever higher than 38,5 degrees C (p<0,0001), chills (p=0,0349), headache (p=0,0499), cloudy urine (p<0,0001), proteinuria (p=0,0011) and positive nitrite-test (p=0,0002). The patients with non-E. coli UTI frequently had fever lower than 38,5 degrees C (p<0,0001) and urine specific gravity <1015 (p=0,0012). There was no significant difference in blood test results between patients with E. coli and non-E. coli UTI. These clinical and laboratory findings can lead us to early etiological diagnosis of these UTI before urine culture detection of causative agents, which takes several days. Early etiological diagnosis of the E. coli and non-E. coli UTI is necessary for an urgent administration of appropriate empirical antibiotic treatment. This is very important in prevention of irreversible kidney damage, prolonged treatment, complications, as well as recidives and chronicity of the illness.


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