scholarly journals Analisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp

2011 ◽  
Vol 11 (1) ◽  
pp. 15-21
Author(s):  
Elmy Mariana
Keyword(s):  
Restriction Enzyme ◽  
Polymorphism Analysis ◽  
Dairy Farmers ◽  
Lactating Cows ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Lactoferrin Gene ◽  
Ecori Restriction

Polymorphism analysis of lactoferrin gene on holstein-friesian cow with PCR-RFLP methodABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene in Holstein-Friesian (HF) cows. The study was conducted on 281 heads of HF lactating cows coming from dairy farmers in Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65.5%) and AB (34.5%) genotypes.

scholarly journals Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein

2011 ◽  
Vol 11 (2) ◽  
pp. 51-57
Author(s):  
Elmy Mariana
Keyword(s):  
Somatic Cells ◽  
Strong Relationship ◽  
Quality Parameters ◽  
Milk Quality ◽  
Dairy Farmers ◽  
Lactating Cows ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Lactoferrin Gene

Association of Lactoferrin Polymhorphism on Milk Yield and Milk Quality in Holstein-Friesian Dairy CowsABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene and the association of genetic variants of the lactoferrin gene on milk quality in Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF lactating cows coming from semi intensive of small dairy farmers in two villages of the KPSBU Lembang, Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65%) and AB (35%) genotypes. Both the AA and AB genotypes had inconsistency in controlling component of the milk quality. Further, the AB lactoferrin cows had a higher frequency in the classification of low somatic cells count (0.75), while the AA cows were at a higher frequency in the classification of high somatic cells count (0.60). It was concluded that there is no strong relationship between intron 6 lactoferrin gene with milk quality parameters. Further, the AB genotype of the lactoferrin gene had a consistency in producing lower somatic cells count compared to the AA genotype.

scholarly journals Genetic Variants of k-Casein and β-Lactoglobulin Genes and Their Association with Protein and Milk Components of Holstein Friesian Cows at Small Farmers in Lembang, West Java

2017 ◽  
Vol 2 (6) ◽  
pp. 86 ◽  
Author(s):  
Anggraeni Anggraeni ◽  
A. Anneke ◽  
H.S. Nury ◽  
E. Andreas ◽  
C. Sumantri
Keyword(s):  
Genetic Variants ◽  
Milk Protein ◽  
Restriction Enzymes ◽  
Dairy Farmers ◽  
West Java ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Milk Components ◽  
Milk Component ◽  
Β Lactoglobulin

Genetic variants of CSN3 and LGB genes and their effects on protein and milk components were studied in Holstein Friesian at small dairy farmers in Lembang District, West Java, Indonesia.  Allelic variants were identified by PCR-RFLP technique using restriction enzymes of Pst I for the CSN3 gene and Hae III for the LGB gene.  The CSN3 gene was dominated by AB genotype.   Milk protein was not affected by genotypes of the two genes.  Only fat content was significantly affected (P <0.05) by the CSN3 gene with AB cows having the highest fat to AA and BB cows (3.76% vs. 3.26% and 3.34%). Keywords: CSN3 gene; LGB gene; milk protein; and milk component.

scholarly journals Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk

2012 ◽  
Vol 51 (No. 1) ◽  
pp. 14-20 ◽  
Author(s):  
K. Wojdak-Maksymiec ◽  
M. Kmiec ◽  
J. Ziemak
Keyword(s):  
Gene Polymorphism ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Bovine Lactoferrin ◽  
Logarithmic Scale ◽  
Black And White ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Lactoferrin Gene

The study included 124 Polish Black-and-White dairy cows of various share of the Holstein-Friesian (HF) breed. Lactoferrin (LTF) gene polymorphism was obtained with PCR-RFLP method using EcoRI enzyme. Two alleles of LTF, A and B, were found in the studied population. Their frequencies were 67.74% and 32.56%, respectively. The alleles controlled the occurrence of three genotypes: AA, BB and AB, of frequencies equal to 37.90%, 2.42% and 59.68%, respectively. It was established that statistically significant associations exist between the somatic cell count (SCC) and LTF genotype, lactation month and parity as well as the HF gene share. No significant association was found between somatic cell count and season. The highest somatic cell count (transformed to a logarithmic scale) was found in milk of the AB genotype, whereas the lowest one was found in cows of the AA genotype.

scholarly journals Factors Determining the Susceptibility of Cows to Mastitis and Losses Incurred by Producers Due to the Disease – A Review

2015 ◽  
Vol 15 (4) ◽  
pp. 819-831 ◽  
Author(s):  
Zygmunt Litwińczuk ◽  
Jolanta Król ◽  
Aneta Brodziak
Keyword(s):  
Mammary Gland ◽  
Living Environment ◽  
Feeding System ◽  
Dry Period ◽  
Dairy Farmers ◽  
Udder Health ◽  
Holstein Friesian ◽  
Increased Risk ◽  
Lactoferrin Gene ◽  
The Cost

Abstract The aim of the paper was to discuss the factors determining the susceptibility of cows to mastitis, the most important for breeders, including losses incurred by milk producers due to the disease. Inflammations of mammary gland are common and the most awkward problem which dairy farmers face around the world. In the case of clinical udder inflammation, it is estimated that the cost of mastitis is about 200 € per cow with fluctuations from 100 even up to 1000 €. Resistance, i.e. the susceptibility of cows to mastitis, is conditioned genetically. The possibility of BoLA and lactoferrin gene polymorphism, using in the selection cows resistant to mastitis, is indicated. Bacteria inhabiting the mammary gland, and the living environment of animals are primarily the main etiological factor. Moreover, many authors indicate the differences between breeds of cows in resistance to mastitis. High-productive breeds are generally more susceptible to the udder inflammation, in comparison to the local breeds. The greatest reduction in milk yield is observed in the case of these breeds, and especially in Holstein-Friesian breed, in which the decline of daily yield at somatic cell count (SCC) amounting above 500,000 per ml of milk can reach 25%. The probability of mastitis occurrence increases with an age of cows and following lactation, and dry period is a time of increased risk. Higher incidence of inflammation is also associated with abnormalities in the construction of the udder (unevenly developed quarters, too long or too short teats). In recent years, a possible relationship between feeding system of cows with their resistance to udder infections has been reported. It is emphasized that a deficiency of selenium and vitamins A and E in the ration may contribute to the increase in the number of mastitis incidences. There are, however, unclear opinions of various authors on the effect of automatic milking system on udder health.

scholarly journals Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1447
Author(s):  
Ishaku L. Haruna ◽  
Yunhai Li ◽  
Ugonna J. Ekegbu ◽  
Hamed Amirpour-Najafabadi ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Nucleotide Sequence ◽  
Milk Fat ◽  
Linear Mixed Effect Model ◽  
Polymorphism Analysis ◽  
Mixed Effect ◽  
Myostatin Gene ◽  
Holstein Friesian ◽  
Nucleotide Sequence Variation

The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

scholarly journals Screening for Hemophilia A Carriers: Utility of PCR-RFLP—Based Polymorphism Analysis

2007 ◽  
Vol 15 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Syed Tasleem Raza ◽  
Nuzhat Husain ◽  
Ashutosh Kumar
Keyword(s):  
Hemophilia A ◽  
Polymorphism Analysis ◽  
Pcr Rflp

scholarly journals Quality Traits of Longissimus thoracis and lumborum and Gluteus medium Muscles from Cull Dairy Holstein-Friesian Cows

2015 ◽  
Vol 4 (6) ◽  
pp. 82
Author(s):  
Carlos Santos ◽  
Carlos Moniz ◽  
Cristina Roseiro ◽  
Vera Medeiros ◽  
Isabel Afonso ◽  
...  
Keyword(s):  
Aging Time ◽  
Gluteus Medius ◽  
Meat Tenderness ◽  
Mean Values ◽  
Lactating Cows ◽  
Holstein Friesian ◽  
The Mean ◽  
Sensorial Attributes ◽  
Main Effects ◽  
Friesian Cows

<em>Longissimus thoracis</em> and <em>lumborum</em> (LTL) and <em>Gluteus medius</em> (Gm) muscles of culled dairy cows, differing in production status (Ps) at slaughter and carcass weight were assessed for intramuscular fat and myoglobin, color, shear force (SF) and sensorial characteristics, after being aged for 2, 7, 14, 28 and 42 days. Meat from dried-off cows was lighter, redder and had higher yellowness and chroma (<em>P </em>&lt; 0.05) than lactating cows. Meat of finished fed cows improved in color in relation to counterparts directly slaughtered, but not significantly. Color parameters increased with aging time and the mean values of LTL and Gm significantly differed mostly among lactating cows. SF of meat was affected by aging time, muscle type, Ps and by their respective interactions (<em>P</em> &lt; 0.001), with a greater impact in LTL muscle. Meat from LTL muscle of dried-off cows had lower SF (47.30 N) than that of lactating cows (65.61 N) (<em>P</em> &lt; 0.05), but such differences were not significant for Gm muscle. Meat tenderness of finished fed cows was higher than counterparts not finished (<em>P</em> &gt; 0.05). Main effects and the results from their interactions were significant for all sensorial attributes (<em>P</em> &lt; 0.001).

scholarly journals Stability of EcoRI Restriction-Modification Enzymes In Vivo Differentiates the EcoRI Restriction-Modification System from Other Postsegregational Cell Killing Systems

2005 ◽  
Vol 187 (19) ◽  
pp. 6612-6621 ◽  
Author(s):  
Asao Ichige ◽  
Ichizo Kobayashi
Keyword(s):  
Restriction Enzyme ◽  
Cell Killing ◽  
Host Cells ◽  
Gene System ◽  
Ecori Restriction ◽  
Differential Stability ◽  
Postsegregational Killing ◽  
Restriction Modification ◽  
Modification Enzyme

ABSTRACT Certain type II restriction modification gene systems can kill host cells when these gene systems are eliminated from the host cells. Such ability to cause postsegregational killing of host cells is the feature of bacterial addiction modules, each of which consists of toxin and antitoxin genes. With these addiction modules, the differential stability of toxin and antitoxin molecules in cells plays an essential role in the execution of postsegregational killing. We here examined in vivo stability of the EcoRI restriction enzyme (toxin) and modification enzyme (antitoxin), the gene system of which has previously been shown to cause postsegregational host killing in Escherichia coli. Using two different methods, namely, quantitative Western blot analysis and pulse-chase immunoprecipitation analysis, we demonstrated that both the EcoRI restriction enzyme and modification enzyme are as stable as bulk cellular proteins and that there is no marked difference in their stability. The numbers of EcoRI restriction and modification enzyme molecules present in a host cell during the steady-state growth were estimated. We monitored changes in cellular levels of the EcoRI restriction and modification enzymes during the postsegregational killing. Results from these analyses together suggest that the EcoRI gene system does not rely on differential stability between the toxin and the antitoxin molecules for execution of postsegregational cell killing. Our results provide insights into the mechanism of postsegregational killing by restriction-modification systems, which seems to be distinct from mechanisms of postsegregational killing by other bacterial addiction modules.

scholarly journals Genetic Polymorphism of SCD1 Gene of Holstein-Friesian Cows in Indonesia

2019 ◽  
Vol 24 (2) ◽  
pp. 56
Author(s):  
Ari Sulistyo Wulandari ◽  
HD Rahayu ◽  
SD Volkandari ◽  
N Herlina ◽  
S Anwar ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Genetic Polymorphism ◽  
Breeding Strategy ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Molecular Selection ◽  
Friesian Cattle ◽  
Selection Of ◽  
Friesian Cows

<p class="abstrak2">Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk. </p><p class="abstrak2"><span><br /></span></p>

scholarly journals Identification of restriction enzyme in the FSHR gene of indonesian local cattle

2021 ◽  
Vol 888 (1) ◽  
pp. 012024
Author(s):  
P W Prihandini ◽  
A Primasari ◽  
M Luthfi ◽  
D Pamungkas ◽  
A P Z N L Sari ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Restriction Site ◽  
Follicle Stimulating Hormone ◽  
Restriction Enzymes ◽  
Future Studies ◽  
Fshr Gene ◽  
Pcr Rflp ◽  
Mapping Analysis ◽  
Pcr Products ◽  
Enzyme Mapping

Abstract The restriction enzyme is important for genotyping using the PCR-RFLP technique. Therefore, this study aims to identify the restriction enzyme mapping in the partial sequence of the follicle-stimulating hormone receptor (FSHR) gene in Indonesian local cattle. A total of 29 samples sized 306 bp, were aligned with Genbank sequence acc no. NC_032660, resulting three polymorphic sites, namely g.193G>C, g.227T>C, and g.275A>C. Furthermore, the restriction mapping analysis using the NEBcutter program V2.0 showed that no enzyme recognized the SNP g.275A>C, while the SNP g.193G>C and g.227T>C were identified by the AluI and MscI enzymes, respectively. The AluI enzyme cuts at two positions (193 bp and 243 bp) in the G allele sample producing three fragments namely 50 bp, 63 bp, and 193 bp, meanwhile, in the C allele, the AluI cuts only in position 243 bp, hence, the fragment products are 63 bp and 243 bp. In contrast, the MscI enzyme was only recognized in the T allele, producing fragments sized 77 bp and 229 bp but failed to identify the restriction site along with the PCR products in the C allele. Based on the results, the SNPs (g.193G>C and g.227T>C) and restriction enzymes (AluI and MscI) are applicable for genotyping local Indonesian cattle using the PCR-RFLP technique in future studies.

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