scholarly journals Efektivitas Sukrosa sebagai Proteksi Aktif Membran Ekstraseluler Spermatozoa Sapi Bali pada Zona Pre-Freezing

2019 ◽  
Vol 19 (1) ◽  
pp. 77-84
Author(s):  
Pajri Anwar ◽  
Jiyanto Jiyanto
Keyword(s):  
Concentration Level ◽  
Sucrose Concentration ◽  
Egg Yolk ◽  
Temperature Changes ◽  
Progressive Movement ◽  
Randomized Design ◽  
Glycerol Treatment ◽  
Active Protection ◽  
Completely Randomized Design ◽  
Membrane Protection

ABSTRAK. Penelitian ini bertujuan untuk mengetahui efektivitas kinerja sukrosa dalam Tris kuning telur sebagai krioprotektan untuk perlindungan aktif ekstraseluler membran plasma utuh spermatozoa sapi Bali. Bahan yang digunakan dalam penelitian ini adalah sperma sapi Bali terpilih dan bahan pengencer semen. Penelitian menggunakan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 4 ulangan. Perlakuan A (Kontrol) = Tris 80%+20% Kuning telur + Gliserol; Perlakuan B = 0,2% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan C = 0,3% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan D = 0,4% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan E = 0,5% Sukrosa + Tris 80%+20% Kuning telur + Griserol. Parameter yang diamati dalam penelitian ini adalah pergerakan progresif aktif spermatozoa dan membran Plasma Utuh (MPU) pada tahap pre-feezing semen sapi Bali. Hasil penelitian menunjukkan bahwa tingkat konsentrasi sukrosa pada tahap pre-freezing tidak berpengaruh nyata (P 0,05%) terhadap persentase progresif aktif spermatozoa dan proteksi MPU semen Sapi Bali dan tingkat aktif progresif spermatozoa dengan penambahan sukrosa dalam kuning telur dikategorikan baik (66.75- 69.00%). Penambahan berbagai level  konsentrasi sukrosa sebagai krioprotektan ekstraseluler melapisi dan mengikat membran spermatozoa dari efek perubahan suhu yang drastis pada tahap pre-freezing dalam proses semen beku. The effectiveness of sucrose as the Active Protection of Bali Spermatozoa Extracellular Membranes Pre-Freezing ABSTRACT. This study aimed to determine the effectiveness of the performance of sucrose in tris egg yolk as a cryoprotectant for extracellular active protection of extracellular membrane of Bali bulls spermatozoa. The material used in this study is the sperm of selected Bali bulls and spermatozoa thinning agent. This study used an experimental method with a completely randomized design (CRD) consisting of 5 treatments and 4 replications. Treatment A (Control) = Tris 80% + 20% Egg Yolk + Glycerol; Treatment B = 0.2% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; Treatment C = 0.3% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; Treatment D = 0.4% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; and Treatment E = 0.5% Sucrose + Tris 80% + 20% Egg Yolk + Griserol. The parameters observed in this study were the active progressive movement and extracellular membrane of spermatozoa protection in Bali bulls pre-freezing stage. The results showed that the sucrose tris egg yolk concentration level at the pre freezing stage no significant effect (P 0.05%) on the active progressive percentage of spermatozoa and bali bulls extracellular membrane protection conditions active levels of progressive spermatozoa with the addition of sucrose in egg yolk are categorized as good (66.75- 69.00%). Addition of various levels of sucrose concentration as extracellular cryoprotectant coating and binding of spermatozoa membranes from the effects of drastic temperature changes in the pre-freezing stage in the process of frozen sperm.

scholarly journals Calla lily inflorescences postharvest: pulsing with different sucrose concentrations and storage conditions

2011 ◽  
Vol 35 (4) ◽  
pp. 657-663 ◽  
Author(s):  
Elka Fabiana Aparecida Almeida ◽  
Patrícia Duarte de Oliveira Paiva ◽  
Luiz Carlos de Oliveira Lima ◽  
Franklin Cordeiro Silva ◽  
Juliana Fonseca ◽  
...  
Keyword(s):  
Sucrose Concentration ◽  
Storage Conditions ◽  
Prior Treatment ◽  
Dry Storage ◽  
Calla Lily ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment ◽  
Cold Chamber ◽  
And Storage

Calla lily is an appreciated specie used for flower arrangements. In spite of its commercial importance, there is little information on calla lily postharvest conservation. Thus, this study aimed to determine the best sucrose concentration for pulsing and cold storage conditions to extend calla lily postharvest durability. Flower stalks were submitted to a pulsing pre-treatment using 2, 4, 8, 12 and 16% sucrose in the solution, for one hour, plus a treatment with direct storage in cold chamber (4ºC), without a prior-treatment. Dry storage or storage in solution with the commercial product Flower® was also tested. A completely randomized design was used with four replicates and three inflorescences per plot. Spathe length and width were daily measured from which the opening and wilting processes were analyzed. It was observed that pulsing with sucrose was efficient in extending calla lily inflorescences opening process and durability. Dry storage for short periods (less than six days) can also be used, but a prior-treatment with 12% sucrose pulsing for one hour or with a water supply for the same period was required. For storage in solution, a pulsing with 5% or 7.5% sucrose was recommended.

scholarly journals EFEK PENAMBAHAN ENZIM (Amylase, Protease, Xylanase) DALAM PAKAN TERHADAP BERAT TELUR DAN DIAMETER KUNING TELUR PADA ITIK CAMPURAN

2021 ◽  
Vol 11 (1) ◽  
pp. 39-47
Author(s):  
Bagus Uda Palgunadi ◽  
Ratna Widyawati ◽  
Dian Ayu Kartika Sari ◽  
Halimah Tusadiah
Keyword(s):  
Egg Yolk ◽  
Egg Weight ◽  
Experimental Animals ◽  
Anova Test ◽  
Average Value ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

The purpose of this research aims to determine the effect of adding enzymes (amylase, protease, xylanase) in feed on egg weight and egg yolk diameter in mixed ducks. The experimental animals used were 24 laying ducks. The design used was a completely randomized design (CRD) with 4 treatments and 6 replications. The four treatments were P0 (Control), P1 (6 ducks with Avizym1502®5gram / kg), P2 (6 ducks with Avizym1502® 10gram / kg), and P3 (6 ducks given Avizym1502). ® 15gram / kg). This treatment is carried out once a day for 9 weeks. Before the treatment, the ducks were adapted for a week. After that, the initial egg weight and yolk diameter were measured at week 0, week 6, and week 9. Data obtained from the results of weighing eggs found an average value of P0 (59.1667 ± 3.9200a) g, P1 5gram / kg (56.1667 ± 2.9268a) g, P2 10gram / kg (60.1667 ± 4.9966a) g, and P3 15gram / kg (67.6667 ± 2.7325ᵇ) g. The results of data from examining the diameter of the egg yolk found an average value of P0 (45.03 ± 1.2956a) mm, P1 5gram / kg (44.35 ± 0.7969a) mm, P2 10gram / kg (45.75 ± 1.5694a) mm, and P3 15gram / kg (48.83 ± 0.7394ᵇ) mm. The data obtained were then analyzed using the ANOVA test which statistically showed that there was a significant difference (P <0.05) between egg weight and yolk diameter in mixed ducks treated with enzymes (amylase, protease, xylanase) in the feed.

scholarly journals Motilitas dan viabilitas spermatozoa semen sexing kambing peranakan etawa (pe) menggunakan metode sedimentasi putih telur dengan pengencer yang berbeda

2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Significant Influence ◽  
Research Method ◽  
Egg Yolk ◽  
Egg White ◽  
Variance Analysis ◽  
Laboratory Research ◽  
Sedimentation Method ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.

scholarly journals KUALITAS MOTILITAS DAN VIABILITAS SPERMATOZOA DARI SEMEN AFKIR SAPI LIMOUSIN PADA PENGENCER SUSU SKIM KUNING TELUR SITRAT DENGAN PENAMBAHAN BERBAGAI KADAR GLUKOSA

2020 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Rudi Irvanto ◽  
Hardijanto Hardijanto ◽  
Widya Paramita ◽  
Suherni Susilowati ◽  
Tita Damayanti L ◽  
...  
Keyword(s):  
Research Design ◽  
Glucose Level ◽  
Skim Milk ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Design ◽  
Completely Randomized Design

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Semen observation was done at 0 hour, 24 hours and 48 hours. Research design used in this study was completely randomized design with faktorial pattern with 5 replicates. Highest result in motility this research was showed at 24 hours with 30% value in glucose 2,5% treatment and 48 hours with 10% value. The lowest result showed in glucose 0% treatment at 24 hours and 0% at 48 hours. Highest result in viability showed on glucose 2,5% treatment with 62,6% value at 24 hours and at 48 hours with 53,4% value. Lowest result in viability showed on glucose 0% treatment with 44,2% value at 24 hours and 31,4% value at 48 hours.

scholarly journals Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
KHAERUDDIN KHAERUDDIN ◽  
JUNAEDI JUNAEDI ◽  
HASTUTI HASTUTI
Keyword(s):  
Ethylene Glycol ◽  
Dimethyl Sulfoxide ◽  
Liquid Nitrogen ◽  
Egg Yolk ◽  
Sperm Cryopreservation ◽  
Randomized Design ◽  
Native Chicken ◽  
Ringer’S Lactate ◽  
Completely Randomized Design

Abstract. Khaeruddin, Junaedi, Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21: 5718-5722. Imported purebred chickens are becoming more popular and a regular staple in Indonesia. Therefore, it is necessary to strengthen conservation efforts to preserve Indonesian chickens, one of which is by means of sperm cryopreservation. This study aimed to determine the effects of the addition of DMSO and different concentrations of ethylene glycol to a Ringer’s lactate egg yolk (RLY)-or coconut water egg yolk (CWY)-based extender on the quality of frozen-thawed Indonesian chicken sperm. This study was used nine Indonesian native roosters about 20 months of age. The semen extenders used in this study were RLY + DMSO 7%, RLY + ethylene glycol 3%, RLY + ethylene glycol 5%, RLY + ethylene glycol 7%, CWY + DMSO 7%, CWY + ethylene glycol 3%, CWY + ethylene glycol 5% and CWY + ethylene glycol 7%. Liquid semen was packaged in 0.25 mL straw, then cooled at 5oC for 2 hours, frozen at 5 cm above liquid nitrogen for 10 minutes, following stored in a liquid nitrogen container for 24 hours. The semen straws were thawed at 37oC for 30 seconds. Statistical analysis for multiple comparisons was performed as a completely randomized design with eight treatment levels and seven replications. The results showed that there were no differences in sperm motility, recovery rate, and abnormality between extenders after the freeze-thaw process. Whereas, RLY + DMSO 7% was the highest sperm viability.

scholarly journals PEMANFAATAN TEPUNG KULIT PEPAYA (Carica papaya L) DALAM RANSUM TERHADAP KUALITAS INTERNAL TELUR

ZOOTEC ◽  
2020 ◽  
Vol 40 (2) ◽  
pp. 418
Author(s):  
Armando Paat ◽  
C.L. Sarajar ◽  
J. R. Leke ◽  
F. N. Sompie
Keyword(s):  
Carica Papaya ◽  
Laying Hens ◽  
Egg Yolk ◽  
Internal Quality ◽  
Egg Weight ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Chicken Eggs

UTILIZATION AND INTERNAL QUALITY OF EGG ON PAPAYA PEEL MEAL DIET (CARICA PAPAYA L). The aim of research was to utilization and internal quality of eggs on papaya (carica papaya L) peel meal diet. The material used in this study were one hundred laying hens (MB 402). The completely randomized design (CRD) was done in this research for analysing of statistics to five treatments and four replications, each replications was occupied by five laying hens (MB 402). The variable measured were egg weight, egg yolk, colour of egg yolk.  The result of this research showed that egg weight, egg yolk, the colour egg yolk was not significantly different (P>0.05). Conclusion, the utilization of flour skin papaya until 16% in feed will increase the quality of chicken eggs. Kata Kunci: Papaya peel meal, laying feed, the quality internal egg.

scholarly journals KULTUR ANTERA ANTHURIUM: PENGARUH SUKROSA DAN GLUKOSA TERHADAP KEBERHASILAN INDUKSI PEMBENTUKAN KALUS DAN REGENERASINYA

2009 ◽  
Vol 14 (2) ◽  
pp. 165-171
Author(s):  
Budi Winarto ◽  
N.A. Mattjik ◽  
A. Purwito ◽  
B. Marwoto
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Sucrose Concentration ◽  
Potential Growth ◽  
Research Institution ◽  
High Effect ◽  
Randomized Design ◽  
Suitable Combination ◽  
Ornamental Crops ◽  
Completely Randomized Design

Effect of sucrose and glucose on callus induction and formation, growth and its regeneration was studied in tissue culture laboratory of Indonesian Ornamental Crops Research Institution from January to November 2009. Objective of this study was to know different combination-concentration of sucrose and glucose on callus induction and formation, growth and its regeneration in anther culture of anthurium. Anthers harvested from spadix with 50% of their pistil in optimal receptive, callus derived from them, Winarto and Rachmawati medium containing 1.5 mg/l TDZ, 0.75 mg/l BAP and 0.02 mg/l NAA were used in the study. Sucrose concentrations were (1) 20 g/l, (2) 40 g/l, (3) 60 g/l dan (4) 80 g/l. While glucose concentrations were (1) 0 g/l, (2) 10 g/l, (3) 30 g/l dan (4) 60 g/l. Variation of the concentrations was applied in callus induction, growth and its regeneration studies. Factorial experiment was arranged by completely randomized design with four replications. Results of this research indicate that existence of sucrose in Winarto and Rachmawati medium gave high effect on successful in antera culture of anthurium and its callus regeneration compared to glucose. Increasing sucrose concentration from 30 g/l to 60 g/l had high impact in callus formation, growth and its regeneration. Sucrose of 60 g/l in combination with 30 g/l glucose was the most suitable combination treatment for callus induction and formation with potential growth of antera up to 92%, 67% antera regeneration and 4.0 calluses formed per replication. The sucrose concentration was also to be the best concentration callus growth and its regeneration with 332 mm3 per callus and 3.2 shoots per explant.

scholarly journals Motility, Viability, Spermatozoa Abnormality, and pH of Bali Cattle Semen in Another-Yellow Water Driller Stored in a Different Time

2021 ◽  
Vol 3 (2) ◽  
pp. 76-90
Author(s):  
Fransiskus X. Manehat ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Positive Impact ◽  
Egg Yolk ◽  
Sugarcane Juice ◽  
Spermatozoa Motility ◽  
Average Value ◽  
Cane Juice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bali Cattle

This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. This research was carried out in the stables and Laboratory of the Faculty of Agriculture, University of Timor in August-September 2020. Semen was collected from an adult male bali cattle, aged ± 4.5 years in healthy condition. The method used in this study is an experimental method using a completely randomized design consisting of 4 treatments and 5 replications. Each treatment was T1: 0.075 ml semen + 0.3 ml of egg yolk sugarcane juice diluent and stored for 24 hours, T2: semen 0.075 ml + 0.3 ml of diluent for egg yolk cane juice and stored for 48 hours, T3: semen 0.075 ml + 0.3 ml of diluent for egg yolk sugarcane juice and stored for 72 hours, T4: semen 0.075 ml + 0 ,3 ml of egg yolk sugarcane juice diluent and stored for 96 hours. The variables measured were individual motility, viability, spermatozoa abnormalities, and semen pH. The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours.    

scholarly journals Characteristics of reduced fat mayonnaise using pumpkin flour (Cucurbita moschata) as fat replacer

2022 ◽  
Vol 335 ◽  
pp. 00017
Author(s):  
Hemas Azizila Nidhal ◽  
Herly Evanuarini ◽  
Imam Thohari
Keyword(s):  
Moisture Content ◽  
Sensory Evaluation ◽  
Emulsion Stability ◽  
Egg Yolk ◽  
Fat Replacer ◽  
Reduced Fat ◽  
Randomized Design ◽  
Experimental Laboratory ◽  
Completely Randomized Design ◽  
Total Oil

Reduced fat mayonnaise has the disadvantage of destabilization of emulsion. Emulsion destabilization that occurs is the separation of oil and water. Pumpkin flour is used to increase the viscosity and stability of the emulsion. The objective of this study was to determine the best percentage of the use of pumpkin flour in reduced fat mayonnaise based on pH, emulsion stability, moisture content, and sensory evaluation. The material used in this research was mayonnaise made from egg yolk, canola flower oil, vinegar, pumpkin flour as a fat replacer carbohidrates based, and other optional ingredients. The method used was an experimental laboratory with a completely randomized design with 4 treatments and 4 replications. The treatments consisted of without the use of pumpkin as a control, the use of pumpkin flour 2%, 4%, and 6% of the total oil used. The variables measured were pH, emulsion stability, moisture content, and sensory evaluation. The results showed that the use of pumpkin flour on mayonnaise gave highly significant effect on pH, stability emulsion, moisture content and increased panelists preferences. The conclusion of the study that using 6% pumpkin flour produced the best reduced-fat mayonnaise.

scholarly journals LIVABILITY AND RECOVERY RATE OF BALI CATTLE SPERMATOZOA DURING PRESERVATION IN TRIS-BASED EGG YOLK DILUENT WITH DIFFERENT SUCROSE LEVELS

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Yendraliza Yendraliza ◽  
Tania Tania ◽  
Restu Misrianti ◽  
Zumarni Zumarni
Keyword(s):  
Plasma Membrane ◽  
Recovery Rate ◽  
Egg Yolk ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bali Cattle

This study aimed to determine the effect of sucrose addition in tris-based egg yolk diluent in maintaining and protecting spermatozoa during preservation. The design of this study was a completely randomized design (CRD) which consisted of five treatments with ten replications. The treatments were tris-based egg yolk diluent without sucrose (P0, control), tris-based egg yolk diluent with 0.2% sucrose (P1), tris-based egg yolk diluent with 0.3% sucrose (P2), tris-based egg yolk diluent with 0.4% sucrose (P3) and tris-based egg yolk diluent with 0.5% sucrose (P4). The parameters measured were motility, livability, abnormality, intact plasma membrane, and recovery rate. Semen was evaluated 2 times, freshly after being collected and post thawed. The results showed that the increase level of sucrose addition from 0.2% to 0.5% increased the motility value from 43.59% to 48.15%, the livability value from 51.24% to 55.45%, the intact plasma membrane value from 44.66% to 48.21%, the recovery rate value from 54.6% to 60.2 %, and reduce the abnormality value from 13.49%-10.24%. It can be concluded that the addition of 0.2- 0.5% sucrose in tris-based egg yolk diluent could increase motility, livability, intact plasma membrane, recovery rate, and could reduce the abnormalities of Bali cattle spermatozoa during preservation

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