Effect of Tobacco on Knee Joint Recovery of College Students After Sports

2021 ◽  
Vol 7 (4) ◽  
pp. 400-407
Author(s):  
Xiaofeng Zhang
Keyword(s):  
College Students ◽  
Knee Joint ◽  
Protective Effect ◽  
Iodoacetic Acid ◽  
Control Group ◽  
Joint Cartilage ◽  
Blank Control Group ◽  
Knee Arthritis ◽  
Osteoarthritis Cartilage ◽  
Histological Changes

Objectives: By analyzing the protective effect and mechanism of tobacco on knee joint cartilage in rats, this paper studies the effect of tobacco on knee joint recovery of college students after sports. Methods: Firstly, the main subunits of nAChRs were systematically studied by using the rat knee arthritis model α 7 and α 4 and β To clarify the correlation between nAChRs and the occurrence and development of OA. Then, the OA rat model prepared by iodoacetic acid was used as the experimental object to observe the protective effect of nicotine on knee osteoarthritis cartilage in rats. Results: The histological changes of rats in MIA group were obvious after operation. The results of light microscope score and Mankin's score at 15 and 30 days were significantly higher than those in con group. Of right knee cartilage in rats in MIA group α 7, α 4 and β The expression of 2 did not change significantly on the 15th day, but increased significantly on the 30th day compared with the blank control group. Conclusion: Nicotine has a protective effect on knee bone and joint cartilage and promotes the accelerated recovery of knee bone and joint after exercise.. Key words: nicotine, knee joint, cartilage, recovery after exercise.

scholarly journals Study on the Protective Effect of Special Electromagnetic Field Treated Water and Far Infrared Rays on LPS-Induced ARDS Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Ruiyin Huang ◽  
Yan Li ◽  
Xin Li ◽  
Qian Wang ◽  
Changyong Luo ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Protective Effect ◽  
Lung Tissue ◽  
Far Infrared ◽  
Inflammatory Factors ◽  
Control Group ◽  
Model Group ◽  
Blank Control Group ◽  
Treated Water ◽  
Infrared Rays

Objective. To explore the protective effect of special electromagnetic field treated water (SEW) and far infrared rays (IFR) on endotoxin (lipopolysaccharide, LPS) induced ARDS rats and the effect on inflammatory factors. Methods. 40 healthy male SD rats were randomly divided into 4 groups with 10 in each group. Preventive experiment: Adaptive feeding was carried out for 1 week according to animal feeding standards. Rats in SEW group drank SEW daily. Samely, rats in SEW and FIR group also drank SEW daily. Besides, rats in SEW and FIR group were also given far infrared rays for 20min/d. Rats in model group drank distilled water daily. After 7 days, rats in each group were injected with LPS (2 mg/kg) via the tail vein for making models. Rats in blank control group were given distilled water for 7 days, without modeling. All rats in the 4 groups were put to death under anesthesia 16 hours after modeling. Lung tissue and abdominal aortic blood were taken from these rats. Results. Pathological observation of lung and lung tissue indicated that rats in model group showed great pathological difference from rats in blank group. Rats in intervention group showed more symptomatic improvement in relation to alveolar and pulmonary interstitial congestion, edema, and inflammatory cell infiltration than rats in model group. The level of inflammatory factors like IL-1β and IL-6 in serum of rats in model group increased compared to blank control group (p<0.05). Comparing SEW group and SEW and FIR group with model group, levels of IL-1β and IL-6 in serum of rats both decreased remarkably (IL-Iβ: P < 0.05; IL-6: P < 0.01) while there was no obvious difference between SEW group and SEW and FIR group (p>0.05). The lung coefficient (LI) in SEW and FIR group was significantly lower than that in model group (P<0.01), which was higher than that of blank control group (P < 0.05), while there was no obvious difference between model group and SEW group (P>0.05). Compared with blank control group, lung permeability index (LPI) in model group showed no obvious difference (P>0.05). Conclusion. Special electromagnetic field treated water and far infrared rays can alleviate lung tissue damage of endotoxin-induced ARDS rats, relieving symptoms of alveolar and pulmonary interstitial congestion, edema, and inflammatory cell infiltration. The protective effect of special electromagnetic field treated water and far infrared rays on endotoxin-induced acute respiratory distress syndrome may result from their role in reducing the levels of IL-1β and IL-6 in serum and the expression level of p65 protein in lung tissue, in addition to reliving inflammatory response, lung coefficient, and lungs edema.

scholarly journals The Effect of Acupoint Application of Sinomenine for Rheumatoid Arthritis Measured by Microdialysis and UPLC-MS/MS

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Rui Wang ◽  
Jing Yang ◽  
Yuxuan Liu ◽  
Bixi Guan ◽  
Qun Feng ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Knee Joint ◽  
Treatment Effects ◽  
Prolonged Treatment ◽  
Control Group ◽  
Blank Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Effect Time ◽  
Joint Group

Objective. This study aimed to investigate the treatment effects of acupoint application of sinomenine in rheumatoid arthritis (RA). RA models were constructed using male New Zealand rabbits. Methods. The rabbits were randomly divided into a blank control group and four experimental groups as follows: ST 36 group (acupoint application of sinomenine at Zusanli); GB 34 group (acupoint application of sinomenine at Yanglingquan); knee-joint group (application directly at the site of the knee joint); and oral administration group (sinomenine administered by gavage). In all rabbits, microdialysis was applied at the knee joint to obtain samples. Pharmacokinetic (PK) and pharmacodynamic (PD) parameters were measured by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and the PK/PD models were established according to the parameters derived. Results. Sinomenine concentration was in the range of 0.832–208 ng/mL, and the peak area showed a good linear relationship with the regression equation of y = 539.64x + 953.81; r = 0.9998. Moreover, good specificity and precision were obtained for the LC-MS/MS method of sinomenine evaluation in the microdialysate samples. The PK analysis showed that the sinomenine effect time was longer in the ST 36 group (area under the time-concentration curve (AUC): 12683.81 h·ng/ml and Tmax: 6.21 h) than in the other groups. Arginine and citrulline were selected as the indices for PD, and in the analysis of parameters for PK/PD, the highest value of Emax and the lowest value of ke0 were obtained in the ST 36 group. Conclusion. Acupoint application of sinomenine at ST 36 has potential for use in patients with RA by enabling enhanced and prolonged treatment effects.

scholarly journals Coculture of allogenic DBM and BMSCs in the knee joint cavity of rabbits for cartilage tissue engineering

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Bin Xu ◽  
Rui Wang ◽  
Hao Wang ◽  
Hong-Gang Xu
Keyword(s):  
Tissue Engineering ◽  
Knee Joint ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Control Group ◽  
Blank Control Group ◽  
Joint Cavity ◽  
A Value

The present study aims to assess coculture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in vitro group, an in vivo group, and a blank control group. At the 4th, 8th, and 12th week, samples from all groups were collected for hematoxylin–eosin (HE) staining and streptavidin–peroxidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value). SP and flow cytometry demonstrated that BMSCs were induced into chondrocytes. DBM scaffold showed honeycomb-shaped porous and three-dimensional structure, while the surface pores are interlinked with the deep pores. At the 4th week, in the blank control group, DBM scaffold structure was clear, and cells analogous to chondrocytes were scattered in the interior of DBM scaffolds. At the 8th week, in the in vivo group, there were a large amount of cells, mainly mature chondrocytes, and the DBM scaffolds were partially absorbed. At the 12th week, in the in vitro group, the interior of scaffolds was filled up with chondrocytes with partial fibrosis, but arranged in disorder. In the in vivo group, the chondrocytes completely infiltrated into the interior of scaffolds and were arranged in certain stress direction. The in vivo group showed higher A value than the in vitro and blank control groups at each time point. Allogenic DBM combined BMSCs in the knee joint cavity of rabbits could provide better tissue-engineered cartilage than that cultivated in vitro.

scholarly journals Animal models of ovariectomy in rats demonstrate characteristics similar to early stage osteoarthritis

2021 ◽  
Author(s):  
Natália Aparecida Casonato ◽  
Camila Marques de Araújo ◽  
Mariane Santos Trevisan ◽  
Cristina Arrais Lima ◽  
Fernando Augusto Vasilceac
Keyword(s):  
Animal Models ◽  
Knee Joint ◽  
Wistar Rats ◽  
Early Stage ◽  
Experimental Period ◽  
Histological Evaluation ◽  
Control Group ◽  
Intermediate Region ◽  
Histological Changes ◽  
Deep Region

Abstract Purpose The aim of our study is to analyze the model of ovariectomy (OVX) in rats reproduced histological changes of osteoarthritis (OA). Methods For the development of the research, 12 Wistar rats were used, divided into 2 equal groups: Control Group - C (n = 6) and Osteoarthritis Group - OA (n = 6). After the 6-month experimental period, all rats were sacrificed and, subsequently, the entire knee joint complex was removed without disarticulation. For the histological evaluation of the tissue, the recommendations of the International Society for Research in OA (OARSI) were used. For data processing, each evaluation was statistically treated in both groups, comparing data from group C with the group OA. Results: Through the histological evaluation of OARSI, the evolution of OA in various tissues of the joint was evaluated. Although the OA group showed noticeable differences from group C, they were not as significant. Thus, only statistically significant favors were presented in the loss of the cartilaginous matrix (OA and C, p = 0.51), considering that the changes in the loss of ECM occurred only at the depth of 0% (superficial region), but at the depth of 50% (intermediate region) and 100% depth (deep region) did not exist. Conclusion Our study demonstrated that the OVX model is a good model to discuss OA, showing histological changes similar to those found in OA, the model demonstrated to have a progressive and slow characteristic since after the OARSI evaluations, prominent evidence was found in the initial manifestations of OA.

scholarly journals A Biomechanical Analysis of the Effect of Pilates Exercise on Female College Students with Knee Joint Deformity

2021 ◽  
Vol 23 (3) ◽  
pp. 20-28
Author(s):  
Okhee Song ◽  
Kook-Eun Seo ◽  
David Michael O’Sullivan ◽  
Jung-Jun Park
Keyword(s):  
College Students ◽  
Knee Joint ◽  
Internal Rotation ◽  
Weight Bearing ◽  
Exercise Group ◽  
Biomechanical Analysis ◽  
Female College Students ◽  
Control Group ◽  
Genu Varum ◽  
Female College

BACKGROUND Current research on genu varum shows the favorable influence of exercise on the biomechanics of the knee joint by reducing the static malalignment and mechanical imbalances, however the transference to a more optimum gait has not been investigated.OBJECTIVES To investigate the effects of Pilates exercise on the changes of gait and lower limb malalignment in female students with genu varum.METHODS A total of 23 female college students with verified genu varum participated in this study. The participants were randomly assigned to two groups a Pilates exercise group (n=15) and a control group (n=8). The Pilates exercise group participated in 1 hour Pilates exercise 3 times per week for a total of 10 weeks. Each of the participants had an X-ray and performed gait 5 times before and after the exercise treatment. The participants kinetic and kinematic data were gathered using an eight Vicon Motion camera system and two force platforms.RESULTS For the Pilates group gait their maximum extension and internal rotation knee moment, and maximum adduction and internal rotation hip moment significantly increased, while the maximum knee moment flexion decreased. For the control group gait their maximum hip extension and hip adduction moment significantly decreased. For the Pilates group there was a significant reduction in the distance from the anatomical axis to the weight bearing line in the left leg, but there was no significant change for the control group.CONCLUSIONS The results suggest that Pilates exercise may be beneficial for females with genu varum by helping to improve both their static alignment and helping their gait to become more balanced.

scholarly journals The employment of cynomolgus monkey as proprioceptive deficit model of the anterior cruciate ligament

2021 ◽  
Vol 29 (2) ◽  
pp. 230949902110155
Author(s):  
Lei Zhang ◽  
Ruihan Wang ◽  
Ping Zhang ◽  
Huan Liu ◽  
Guoyou Wang ◽  
...  
Keyword(s):  
Knee Joint ◽  
Latent Period ◽  
Pivot Shift ◽  
Treatment Strategies ◽  
Control Group ◽  
Model Group ◽  
Blank Control Group ◽  
Deficit Model ◽  
Significant Difference ◽  
Proprioceptive Deficit

Introduction: Clinically, a consensus of the treatment strategies of ACL grade 2 injury is not reached. Therefore, the present study established a proprioceptive deficit model of the ACL via an arthroscope to aid with further studying ACL grade 2 injury and treatment strategies. Materials and Methods: 12 cynomolgus monkeys were randomly divided into the model group and the blank control group. In the model group, 1/4 of the ACL was cut under an arthroscope, whereas no intervention was performed in the blank control group. Physiological data including the maximum degree of knee flexion, the thigh circumstance and the calf circumference were measured, and the Pivot-shift, Anterior and Lachman tests were also performed. Moreover, electrophysiological data including somatosensory evoked potentials (SEPs) and motor nerve conduction velocity (MNCV) were measured. SEPs and MNCV were assessed for the latent period and amplitude. Results: Comparing the data before and after the surgery, in the blank control group, no significant difference was observed. In the model group, significant difference was observed in the Pivot-shift, Anterior drawer and Lachman test ( p < 0.05), indicating the instability of the ACL. Moreover, the latent period of SEPs and MNCV were significantly increased ( p < 0.009), whereas the amplitude of SEPs and MNCV was significantly decreased ( p < 0.009), indicating a degenerated proprioception of the knee joint. Conclusions: Following cutting 1/4 of the ACL, the knee joint became unstable and proprioception was declined. The results indicated that the proprioceptive deficit model of the ACL was successfully established, which could promote further studying ACL injury.

Physiological Reloading Recovers Histologically Disuse Atrophy of the Articular Cartilage and Bone by Hindlimb Suspension in Rat Knee Joint

Cartilage ◽  
2021 ◽  
pp. 194760352110638
Author(s):  
Ikufumi Takahashi ◽  
Taro Matsuzaki ◽  
Hiroshi Kuroki ◽  
Masahiro Hoso
Keyword(s):  
Articular Cartilage ◽  
Knee Joint ◽  
Bone Volume ◽  
Male Rats ◽  
Hindlimb Suspension ◽  
Control Group ◽  
Disuse Atrophy ◽  
Histological Changes ◽  
Suspension Model ◽  
Immunohistochemical Analyses

Objective This study aimed to clarify physiological reloading on disuse atrophy of the articular cartilage and bone in the rat knee using the hindlimb suspension model. Design Thirty male rats were divided into 3 experimental groups: control group, hindlimb suspension group, and reloading after hindlimb suspension group. Histological changes in the articular cartilage and bone of the tibia were evaluated by histomorphometrical and immunohistochemical analyses at 2 and 4 weeks after reloading. Results The thinning and loss of matrix staining in the articular cartilage and the decrease in bone volume induced by hindlimb suspension recovered to the same level as the control group after 2 weeks of reloading. The proportion of the noncalcified and calcified layers of the articular cartilage and the thinning of subchondral bone recovered to the same level as the control group after 4 weeks of reloading. Conclusions Disuse atrophy of the articular cartilage and bone induced by hindlimb suspension in the tibia of rats was improved by physiological reloading.

scholarly journals The Effect of an 8-Week Hamstring Weight Training on H/Q ratio and H/Q FI of Male College Students

2021 ◽  
Vol 30 (3) ◽  
pp. 295-302
Author(s):  
Seung-Ri Kim ◽  
Hyun-Joo Kang
Keyword(s):  
College Students ◽  
Knee Joint ◽  
Weight Training ◽  
Statistical Significance ◽  
Peak Torque ◽  
Control Group ◽  
Significant Difference ◽  
Male College ◽  
The Difference ◽  
Male College Students

PURPOSE: This study aimed to examine the effects on the hamstring/quadriceps ratio (H/Q ratio) and H/Q ratio fatigue index (H/Q FI) of the knee joint of an 8-week weight training to improve the hamstring muscles of male college students.METHODS: Twenty male college students were divided into two groups: an exercise group (EG) and a control group (CG). The EG participated in an 8-week hamstring weight training program, working out twice a week, 60 min per session, with a 60–80% 1RM and 12–16 RPE intensity. The peak torque, H/Q ratio, and H/Q FI were measured, and a t-test was used to verify the statistical significance of the difference between the pre- and post-intervention results.RESULTS: The right flexor peak torque at 60°/sec increased by 14.10±9.60%BW in the EG and decreased by −9.00±16.55%BW in the CG, showing a significant difference between the groups (<i>p</i><0.01). The left flexor peak torque increased by 6.60±12.31%BW in the EG and decreased by −5.10±10.68%BW in the CG, showing a significant difference between the groups (<i>p</i><0.05). The left extensor peak torque decreased by −5.40±19.53%BW in the EG and by −28.40±25.70%BW in the CG, showing a significant difference between the groups (<i>p</i><0.05). The H/Q ratios tended to increase, but the difference was not significant.CONCLUSIONS: To prevent injuries and stabilize the knee joint, the central joint of the lower extremity that plays an important role in daily life and sports activities, hamstring weight training can be performed to maintain the H/Q ratio within the normal range.

Protective Effect of Boswellic Resin Against Memory Loss and Alzheimer’s Induced by Aluminum Tetrachloride and D-Galactose (Experimental study in Mice)

2020 ◽  
Author(s):  
K. Zerrouki ◽  
N. Djebli ◽  
L. Gadouche ◽  
I. Erdogan Orhan ◽  
F. SezerSenol Deniz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Protective Effect ◽  
Cell Damage ◽  
Memory Loss ◽  
Control Group ◽  
Total Extract ◽  
Swiss Mice ◽  
Octyl Acetate

Nowadays, because of the industrialization, a lot of contaminant were available ; the consequences of this availability are apparition of diseases including neurodegeneration. Neurodegenerative diseases of the human brain comprise a variety of disorders that affect an increasing percentage of the population. This study is based on the effect of the Boswellic resin, which is from a medicinal plant and known for its antioxidant effects on nerve cell damage. The objective of this work was to evaluate the in vitro and in vivo effects of the Boswellic resin on anticholinesterase activity and Alzheimer’s disease (AD) induced by D-galactose and aluminum tetrachloride in Swiss mice. Chemical composition of the resin essential oil was identified by the CG-MS analysis. The antioxidant activity was also assessed by the DMPD and metal chelation methods. In order to understand the mechanism of memory improvement, the acetylcholinesterase, AChE, and butyrylcholinesterase, BChE, inhibitory assays were performed. In vivo part of the study was achieved on Swiss mice divided into four groups: control, AD model, treated AD, and treated control group. The identification of chemical composition by CG-MS reach the 89.67% of the total extract compounds presented some very important molecules (p-Cymene, n-Octyl acetate, α-Pinene…). The present study proves that Boswellic resin improves memory and learning in treated Alzheimer’s group, modulates the oxidative stress and be involved in the protective effect against amyloid deposition and neurodegeneration, and stimulates the immune system in mice’s brain.

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