scholarly journals The Effect of Acupoint Application of Sinomenine for Rheumatoid Arthritis Measured by Microdialysis and UPLC-MS/MS

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Rui Wang ◽  
Jing Yang ◽  
Yuxuan Liu ◽  
Bixi Guan ◽  
Qun Feng ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Knee Joint ◽  
Treatment Effects ◽  
Prolonged Treatment ◽  
Control Group ◽  
Blank Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Effect Time ◽  
Joint Group

Objective. This study aimed to investigate the treatment effects of acupoint application of sinomenine in rheumatoid arthritis (RA). RA models were constructed using male New Zealand rabbits. Methods. The rabbits were randomly divided into a blank control group and four experimental groups as follows: ST 36 group (acupoint application of sinomenine at Zusanli); GB 34 group (acupoint application of sinomenine at Yanglingquan); knee-joint group (application directly at the site of the knee joint); and oral administration group (sinomenine administered by gavage). In all rabbits, microdialysis was applied at the knee joint to obtain samples. Pharmacokinetic (PK) and pharmacodynamic (PD) parameters were measured by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and the PK/PD models were established according to the parameters derived. Results. Sinomenine concentration was in the range of 0.832–208 ng/mL, and the peak area showed a good linear relationship with the regression equation of y = 539.64x + 953.81; r = 0.9998. Moreover, good specificity and precision were obtained for the LC-MS/MS method of sinomenine evaluation in the microdialysate samples. The PK analysis showed that the sinomenine effect time was longer in the ST 36 group (area under the time-concentration curve (AUC): 12683.81 h·ng/ml and Tmax: 6.21 h) than in the other groups. Arginine and citrulline were selected as the indices for PD, and in the analysis of parameters for PK/PD, the highest value of Emax and the lowest value of ke0 were obtained in the ST 36 group. Conclusion. Acupoint application of sinomenine at ST 36 has potential for use in patients with RA by enabling enhanced and prolonged treatment effects.

Determination of three unsaturated fatty acids in pressure ulcer rats using an UPLC-MS/MS method

2020 ◽  
Vol 16 ◽  
Author(s):  
Fuman Cai ◽  
Yuwei Dong ◽  
Shaosheng Lou ◽  
Zeping Ma ◽  
Ting Wu ◽  
...  
Keyword(s):  
Ammonium Acetate ◽  
Unsaturated Fatty Acids ◽  
Serum Levels ◽  
Ultra Performance Liquid Chromatography ◽  
Release Time ◽  
Control Group ◽  
Serum Concentrations ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Background: The serum levels of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (AA) under the state of pressure ulcers (PUs) are still unclear. Introduction: In order to investigate serum levels of DHA, EPA, and AA in PUs rats, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed and validated. Methods: Chromatographic separation of DHA, EPA, AA was carried out on a BEH C18 column and gradient elute consisted of 5 mM ammonium acetate-0.1% formic acid and acetonitrile. Subsequently, fifty rats were divided into five groups (n=10), four PU groups (A-D) underwent various pressure and release time protocols, with group E as the control. The concentrations of DHA, EPA, AA from five groups were determination using validated method. Results: The results showed there was good linearity for DHA (327.3/283.4), EPA (301.2/257.0), and AA (303.1/258.9) within 0.05-6.4 μg/mL. In control group, the levels of DHA, AA and EPA were 1.16±0.68, 0.59±0.19 and 0.78±0.21 μg/mL. At the end of modeling, concentrations of DHA, EPA and AA were increased after long and persistent pressure (>8 h). Especially, the level of DHA was significantly higher (P<0.01) than that of control group. Conclusion: A stable, reliable and accurate UPLC-MS/MS for determination of DHA, EPA, AA in blood was developed. Serum concentrations of DHA, EPA and AA were altered differently after long and persistent pressure (>8 h), and DHA is a remarkable one.

Effect of Tobacco on Knee Joint Recovery of College Students After Sports

2021 ◽  
Vol 7 (4) ◽  
pp. 400-407
Author(s):  
Xiaofeng Zhang
Keyword(s):  
College Students ◽  
Knee Joint ◽  
Protective Effect ◽  
Iodoacetic Acid ◽  
Control Group ◽  
Joint Cartilage ◽  
Blank Control Group ◽  
Knee Arthritis ◽  
Osteoarthritis Cartilage ◽  
Histological Changes

Objectives: By analyzing the protective effect and mechanism of tobacco on knee joint cartilage in rats, this paper studies the effect of tobacco on knee joint recovery of college students after sports. Methods: Firstly, the main subunits of nAChRs were systematically studied by using the rat knee arthritis model α 7 and α 4 and β To clarify the correlation between nAChRs and the occurrence and development of OA. Then, the OA rat model prepared by iodoacetic acid was used as the experimental object to observe the protective effect of nicotine on knee osteoarthritis cartilage in rats. Results: The histological changes of rats in MIA group were obvious after operation. The results of light microscope score and Mankin's score at 15 and 30 days were significantly higher than those in con group. Of right knee cartilage in rats in MIA group α 7, α 4 and β The expression of 2 did not change significantly on the 15th day, but increased significantly on the 30th day compared with the blank control group. Conclusion: Nicotine has a protective effect on knee bone and joint cartilage and promotes the accelerated recovery of knee bone and joint after exercise.. Key words: nicotine, knee joint, cartilage, recovery after exercise.

scholarly journals Coculture of allogenic DBM and BMSCs in the knee joint cavity of rabbits for cartilage tissue engineering

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Bin Xu ◽  
Rui Wang ◽  
Hao Wang ◽  
Hong-Gang Xu
Keyword(s):  
Tissue Engineering ◽  
Knee Joint ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Control Group ◽  
Blank Control Group ◽  
Joint Cavity ◽  
A Value

The present study aims to assess coculture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in vitro group, an in vivo group, and a blank control group. At the 4th, 8th, and 12th week, samples from all groups were collected for hematoxylin–eosin (HE) staining and streptavidin–peroxidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value). SP and flow cytometry demonstrated that BMSCs were induced into chondrocytes. DBM scaffold showed honeycomb-shaped porous and three-dimensional structure, while the surface pores are interlinked with the deep pores. At the 4th week, in the blank control group, DBM scaffold structure was clear, and cells analogous to chondrocytes were scattered in the interior of DBM scaffolds. At the 8th week, in the in vivo group, there were a large amount of cells, mainly mature chondrocytes, and the DBM scaffolds were partially absorbed. At the 12th week, in the in vitro group, the interior of scaffolds was filled up with chondrocytes with partial fibrosis, but arranged in disorder. In the in vivo group, the chondrocytes completely infiltrated into the interior of scaffolds and were arranged in certain stress direction. The in vivo group showed higher A value than the in vitro and blank control groups at each time point. Allogenic DBM combined BMSCs in the knee joint cavity of rabbits could provide better tissue-engineered cartilage than that cultivated in vitro.

Circulating free nitrotyrosine in obstructive sleep apnea

2004 ◽  
Vol 287 (2) ◽  
pp. R284-R287 ◽  
Author(s):  
Anna Svatikova ◽  
Robert Wolk ◽  
Hui H. Wang ◽  
Maria E. Otto ◽  
Kevin A. Bybee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Nitrosative Stress ◽  
Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Obstructive Sleep ◽  
Before And After ◽  
Liquid Chromatography Tandem Mass ◽  
And Oxidative Stress

Obstructive sleep apnea (OSA) has been increasingly linked to cardiovascular disease, endothelial dysfunction, and oxidative stress, generated by repetitive nocturnal hypoxemia and reperfusion. Circulating free nitrotyrosine has been reported as a novel biomarker of nitric oxide (NO)-induced oxidative/nitrosative stress. Nitrosative stress has been implicated as a possible mechanism for development of cardiovascular diseases. We tested the hypothesis that repetitive severe hypoxemia resulting from OSA would increase NO-mediated oxidative stress. We studied 10 men with newly diagnosed moderate to severe OSA who were free of other diseases, had never been treated for OSA, and were taking no medications. Nitrotyrosine measurements, performed by liquid chromatography-tandem mass spectrometry, were made before and after untreated apneic sleep. We compared free nitrotyrosine levels in these patients with those obtained at similar times in 10 healthy male control subjects without OSA, with similar age and body mass index. Evening baseline nitrotyrosine levels were similar before sleep in the control and OSA groups [0.16 ± 0.01 and 0.15 ± 0.01 ng/ml, respectively, P = not significant (NS)]. Neither normal nor disturbed apneic sleep led to significant changes of plasma nitrotyrosine (morning levels: control group 0.14 ± 0.01 ng/ml; OSA group 0.15 ± 0.01 ng/ml, P = NS). OSA was not accompanied by increased circulating free nitrotyrosine either at baseline or after sleep. This observation suggests that repetitive hypoxemia during OSA does not result in increased NO-mediated oxidative/nitrosative stress in otherwise healthy subjects with OSA.

scholarly journals Lipids and Atherogenic Indices Fluctuation in Rheumatoid Arthritis Patients on Long-Term Tocilizumab Treatment

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Fabio Cacciapaglia ◽  
Maria Grazia Anelli ◽  
Angela Rinaldi ◽  
Marco Fornaro ◽  
Giuseppe Lopalco ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Activity Index ◽  
Joint Damage ◽  
Joint Disease ◽  
Atherogenic Index ◽  
Prolonged Treatment ◽  
Control Group ◽  
Long Term Treatment

Rheumatoid arthritis (RA) patients are at high risk of cardiovascular (CV) events, and the chronic inflammatory state may generate quantitative and qualitative changes in lipoprotein fractions. The anti-IL-6 receptor tocilizumab (TCZ), even if effective in inflammation and joint damage prevention, determined significant alterations to RA patients’ lipid levels in randomized controlled trials, but real-world data are lacking. We evaluated the changes in lipid fraction levels and disease activity in a longitudinal cohort of RA patients on long-term treatment with tocilizumab (TCZ) in a community setting. We retrospectively selected 40 naïve-biologic RA patients on treatment with intravenous TCZ compared to 20 RA patients on methotrexate treatment as the control group. Total cholesterol (Tot-Chol), low-density lipoproteins (LDL), high-density lipoprotein (HDL), and triglyceride (TG) levels were measured at the baseline and at 12, 24, and 52 weeks thereafter. At the same points, 28-joint disease activity score (DAS28), clinical disease activity index (CDAI), and EULAR clinical responses were also assessed. During the first 24 weeks, we observed in TCZ-treated patients a progressive statistically significant (p<0.001) increase in Tot-Chol, LDL, HDL, and TG, which returned close to the baseline at 52 weeks. But no changes in the lipid-related CV risk indices Tot-Chol/HDL and LDL/HDL ratios and the atherogenic index (log10 TG/HDL) were detectable. Notably, we observed a statistically significant negative correlation between changes in lipid fractions and DAS28 or CDAI. The prolonged treatment with TCZ was associated to a transient increase in cholesterol’s fractions during the first 6 months of treatment, with inverse correlation to disease activity, but with no impact on surrogate lipid indices of atherogenic risk. These findings may aid clinicians in interpreting the RA patient’s lipid profile in daily clinical practice.

Altered methylarginine levels after surgery in subjects with multinodular goiter

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Suheyla Karaman ◽  
Abdullah Sivrikaya ◽  
Duygu Eryavuz Onmaz ◽  
Husnu Alptekin
Keyword(s):  
Cardiovascular Diseases ◽  
Multinodular Goiter ◽  
Common Type ◽  
Control Group ◽  
Thyroid Disorders ◽  
Diagnosis And Prognosis ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Thyroid Hormone Levels ◽  
Dimethyl Arginine

Abstract Objectives Thyroid disorders are important risk factor for cardiovascular diseases. Levels of methylarginines such as asymmetric dimethyl arginine (ADMA), L-monomethyl arginine (L-NMMA), symmetric dimethyl arginine (SDMA) are increase in cardiovascular diseases. Multinodular goiter (MNG) is the most common type of goiter in adults. To date, no study has been conducted to determine the levels of methylarginine in euthyroid MNG patients. Our aim in this study is to compare levels of methylarginines and related metabolites in the preoperative, postoperative MNG patients and controls. Methods Serum ADMA, SDMA, L-NMMA, homoarginine (hArg), arginine and citrulline concentrations were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results ADMA (p<0.001), L-NMMA (p=0.002), l-arginine (p=0.006) and citrulline (p<0.001) levels were statistically significantly higher in preop group than postop group. ADMA (p=0.003), L-NMMA (p=0.003) levels were statistically significantly higher and SDMA/ADMA (p<0.001), hArg/ADMA (p<0.001) levels were statistically significantly lower in preop group than control group. Conclusions The levels of methylarginines and related metabolites altered in the euthyroid MNG patients compared to the control group, and more importantly, there were significant differences between the preop and postop groups. Therefore, these metabolites can be useful in the diagnosis and prognosis of thyroid disorders, even if thyroid hormone levels are normal.

scholarly journals The employment of cynomolgus monkey as proprioceptive deficit model of the anterior cruciate ligament

2021 ◽  
Vol 29 (2) ◽  
pp. 230949902110155
Author(s):  
Lei Zhang ◽  
Ruihan Wang ◽  
Ping Zhang ◽  
Huan Liu ◽  
Guoyou Wang ◽  
...  
Keyword(s):  
Knee Joint ◽  
Latent Period ◽  
Pivot Shift ◽  
Treatment Strategies ◽  
Control Group ◽  
Model Group ◽  
Blank Control Group ◽  
Deficit Model ◽  
Significant Difference ◽  
Proprioceptive Deficit

Introduction: Clinically, a consensus of the treatment strategies of ACL grade 2 injury is not reached. Therefore, the present study established a proprioceptive deficit model of the ACL via an arthroscope to aid with further studying ACL grade 2 injury and treatment strategies. Materials and Methods: 12 cynomolgus monkeys were randomly divided into the model group and the blank control group. In the model group, 1/4 of the ACL was cut under an arthroscope, whereas no intervention was performed in the blank control group. Physiological data including the maximum degree of knee flexion, the thigh circumstance and the calf circumference were measured, and the Pivot-shift, Anterior and Lachman tests were also performed. Moreover, electrophysiological data including somatosensory evoked potentials (SEPs) and motor nerve conduction velocity (MNCV) were measured. SEPs and MNCV were assessed for the latent period and amplitude. Results: Comparing the data before and after the surgery, in the blank control group, no significant difference was observed. In the model group, significant difference was observed in the Pivot-shift, Anterior drawer and Lachman test ( p < 0.05), indicating the instability of the ACL. Moreover, the latent period of SEPs and MNCV were significantly increased ( p < 0.009), whereas the amplitude of SEPs and MNCV was significantly decreased ( p < 0.009), indicating a degenerated proprioception of the knee joint. Conclusions: Following cutting 1/4 of the ACL, the knee joint became unstable and proprioception was declined. The results indicated that the proprioceptive deficit model of the ACL was successfully established, which could promote further studying ACL injury.

scholarly journals Plasma Free Amino Acid and Carnitine Levels in Pregnant Women with Hydatidiform Mole: A Case-Controlled Study

2021 ◽  
Author(s):  
Hacer Uyanıkoğlu ◽  
İsmail Koyuncu ◽  
Sibel sak ◽  
adnan incebiyik ◽  
nese gul hilali ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pregnant Women ◽  
Free Amino Acid ◽  
Free Amino ◽  
Venous Blood ◽  
Hydatidiform Mole ◽  
Controlled Study ◽  
Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective: To compare plasma free amino acid (FAA) and carnitine levels in pregnant women with and without hydatidiform mole (HM). Methods: This prospective study was conducted in patients admitted to Harran University Medical Faculty Hospital Obstetrics Clinic between January and November 2018. Twenty-three pregnant women with HM, and 24 healthy pregnant women as controls were enrolled in the study. The venous blood samples were collected, and, FAA and carnitine concentrations were measured in plasma using liquid chromatography/tandem mass spectrometry (LC-MS/MS). Results: The levels of alanine, arginine, and valine from the 14 amino acids examined were significantly lower in the HM group than in the healthy group (475.46 ± 103.55 µmol/L vs 556.99 ± 105.16 µmol/L, p = 0.019; 428.87 ± 138.91 µmol/L vs 547.11 ± 140.20 µmol/L, p = 0.009; and 290.96 ± 97.10 µmol/L vs 358.61 ± 75.40 µmol/L, p = 0.03; respectively). In addition, several carnitines, C8DC, C16:1, and C18, of the 27 carnitines examined were significantly higher in the HM group than in the control group (0.07 ± 0.06 µmol/L vs 0.03 ± 0.01 µmol/L, p = 0.021; 0.18 ± 0.09 µmol/L vs 0.10 ± 0.06 µmol/L, p = 0.03; and 0.07 ± 0.02 µmol/L vs 0.03 ± 0.01 µmol/L, p = 0.021; respectively). Conclusion: This study demonstrated that a decrease in some plasma FAAs and an increase in some plasma carnitine levels might be effective in the pathogenesis of HM.

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